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Wang L.,Tobacco Research Institute of CAAS | Cao J.,Tobacco Research Institute of CAAS | Yu W.,Tobacco Research Institute of CAAS | Wang Y.,Tobacco Research Institute of CAAS | And 3 more authors.
Tobacco Science and Technology

A method for determining the nitrite in flue-cured tobacco with ultraviolet-visible spectrophotometry was established. The factors in extraction process, including extraction decolorization, extraction effect, cleansing, and injection rate in determination were tested. Meantime, the stability, precision and accuracy of this method were investigated, and the nitrite contents in different flue-cured tobacco samples were determined by this method. The results showed that this method was simple in operation, featured good stability, high precision and wide applicability with the recovery from 84.8% to 98.2%, and was suitable for the determination of nitrite in flue-cured tobacco. Source

Wang L.,China Tobacco Machinery Group Co. | Shi Y.,Xuchang Tobacco Machinery Co. | Niu B.,China Pingmei Shenma Group Yangguang Property Ltd Company | Zhou H.,China Tobacco Guizhou Industrial Co.
Tobacco Science and Technology

To prevent cigarettes from dropping and wrinkling in the elevator in YF17 cigarette storing and conveying system, the flow rate matching and relative movement of cigarettes in elevating passage were analyzed, which indicated that the effective width at the turning section varied while cigarettes travelling through, and the flow rate of cigarettes could not be well matched by a fixed section passage. Therefore, the elevator was improved as following: adopting a swing-link flexible conveying device at the inlet turning section of elevator, controlling vertical elevating section and inlet turning section separately, and adopting resilient conveying device at the outlet turning section. The results of application showed that the cigarette flow rate was precisely controlled and flexibly conveyed, cigarette dropping was completely avoided, the wrinkled cigarettes reduced from 3-4 to 0.5-1.0 cig./minute, and the appearance quality of cigarette was effectively improved. Source

Peng Q.,Guizhou University | Peng Q.,China Tobacco Guizhou Industrial Co. | Dong R.,Guizhou University | Yang M.,Guizhou University | Geng P.,Guizhou University
Flavour and Fragrance Journal

Volatile phenols have been considered as a class of highly flavour-active compounds in non-alcoholic and alcoholic beverages. However, the information on volatile phenols in Chinese liquors remains limited. This article presents a high-performance liquid chromatography with a fluorescence detector (HPLC-FLD) method for the determination of 10 volatile phenols in Chinese liquors. The method was based on the fluorescent nature of volatile phenols, the separation of the isomers p-cresol and m-cresol with β-cyclodextrin as the resolving agent, and the use of a protective barrier layer for the capture of volatile phenols during the concentration process of the extract solution. The effects of several experimental parameters on recoveries of target volatile phenols were investigated. The correlation coefficients (R2) for calibration curves of the 10 phenols studies were in the range 0.9991-0.9998 when the linearity range was from 0.05 to 4μg/ml. The proposed RP-HPLC method was successfully applied to the analysis of Chinese liquors and the recoveries of the phenols were in the range 91.5-100.4% with RSD 0.9-2.2%, and the limits of detection were 0.0072-0.0234μg/ml. Volatile phenols of different types and in differing amounts were found in several Chinese liquors. The method could be of use for routine evaluation of the quantity of volatile phenols during the production and ageing process of Chinese liquors. © 2013 John Wiley & Sons, Ltd. Source

Cao B.,CAS Zhengzhou Research Institute | Zhang Q.,CAS Zhengzhou Research Institute | Ji H.,China Tobacco Guizhou Industrial Co. | Liu J.,CAS Zhengzhou Research Institute | And 3 more authors.
Analytical and Bioanalytical Chemistry

A hydrophilic-interaction liquid chromatography-tandem mass spectrometry (HILIC-MS-MS) method was developed for the determination of 4- (methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and its metabolites in mouse liver and lung. The limits of detection of all analytes were in the range 0.017-0.057 ng mL-1, and recovery ranged from 88.4-119.8 % with intra and inter-day precision in the range 0.89-6.03 % and 1.01-6.97 %, respectively. This simple and accurate method was used to evaluate the effect of chronic alcohol consumption on NNK bioactivation in mouse tissue. Time-course curves for NNK and its metabolites were generated, and the areas under the curves (AUCs) were compared. It was found that target tissues of NNK carcinogenesis in C57BL/6 mice contained high levels of α-hydroxylation metabolites of NNK and its carbonyl reduction metabolite, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL). The most pronounced effect of alcohol was to enhance α-hydroxylation of NNK in mouse lung and liver, which suggests that chronic alcohol consumption may increase the risk of carcinogenicity associated with NNK in mice. © 2014 Springer-Verlag Berlin Heidelberg. Source

Ma Y.,China Tobacco Guizhou Industrial Co. | Yang B.,Jiangsu Hengsen Tobacco Mechanical Co. | Hang J.,Nanjing Tuzhi Automation Technology Co.
Tobacco Science and Technology

Traditional belt tobacco sorting device cannot reject heavy foreign matter effectively, a sorting system incorporating vertical pneumatic separation and spectral sorting technology was designed. The system was mainly composed of a vibrating conveyor, a pneumatic separating chamber, vertical pneumatic conveying duct, spectral detecting and rejecting unit, material-air separation device. Visible light and near infrared multispectral image processing was adopted to identify foreign matter and greenish strips. An open type detection channel with balanced airflow was designed to improve image clarity. Via the classification model developed from the spectral characteristics of strips together with the rejection protection algorithm based on token bucket, the classification and rejection of heavy and light foreign matters were achieved. A total of 150 foreign matters, light or heavy, were uniformly mixed into the blended strips of brand "Huangguoshu (soft 02)" to validate the separation performance and rejection rate of the system. The results showed that: 1) The moisture, fines and dust contents in strips and the percentage of large and middle size strips were not affected by pneumatic conveying basically. 2) With the total foreign matter rejection rate of 89%, the rejection rates of the system for heavy and light foreign matters were more than 96% and 85%, respectively. 3) The designed open detection channel demanded less cleaning work. The system combined pneumatic separation with spectral sorting, it promoted the rejection rate for foreign matters. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved. Source

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