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Kong H.,China Tobacco Guangdong Industrial Ltd Corporation | Chen C.,China Tobacco Guangdong Industrial Ltd Corporation | Peng L.,Sun Yat Sen University | Gan F.,Sun Yat Sen University
Journal of Chromatography A | Year: 2011

Stepwise key spectrum selection (SKSS) was introduced to resolve batch overlapping peaks from gas chromatography-mass spectrometry (GC-MS) analysis of ten batch tobacco flavoring samples in different storage times. Resolution was implemented on a software platform that embedded the SKSS method. The data from GC-MS analysis of the samples were saved and prepared in ASCII files and then were inputted into the software platform for visual inspections. The data segment with overlapping peaks was precut for subsequent analysis. Spectral background in the data was removed using a linear fitting of the baseline. Four components in the overlapping peaks were automatically detected by the SKSS method. The resolution of the concentration profiles and spectra of the four components was conducted by setting only one parameter, the negative area ratio, as 0.01. The fixed size moving window evolving factor analysis and evolving factor analysis were applied to validate the resolved concentration profiles. The resolved mass spectra were validated by the searched standard through library search at the pure component regions revealed by the resolved concentration profiles. The results showed that the SKSS method could be a simple but powerful tool in resolving batch chromatographic overlapping peaks. © 2011 Elsevier B.V.


Zhang Y.,CAS Zhengzhou Research Institute | Yang X.,CAS Zhengzhou Research Institute | Zhang S.,China Tobacco Guangdong Industrial Corporation | Tian Y.,CAS Zhengzhou Research Institute | And 2 more authors.
Journal of Soil Science and Plant Nutrition | Year: 2013

Four types of soil (red, yellow, cinnamon, and paddy soil) from China were used to conduct experiments in pots to study the influence of humic acid (humic acid (HA) and fulvic acid (FA)) on the accumulation of lead (Pb) and cadmium (Cd) in tobacco leaves. In non-polluted soils, the tobacco leaf uptake of Cd was dramatically influenced by the total soil metal concentration and soil properties, with no relationship observed for Pb. However, in soils that were polluted with 500 mg kg-1 of Pb or10 mg kg-1 of Cd, negative relationships were observed between the soil pH and the metal concentration in the tobacco leaves. When the soil was polluted with Pb, the application of 4 g kg-1 humic acids (HA or FA) decreased the leaf Pb concentration by 17.78%-48.32% in the acidic red and paddy soils but increased the concentration by 11.69%-37.54% in the alkaline cinnamon soil. A 8.74%-32.84% decrease in the leaf Cd concentration occurred in the acidic soils due to 4 g kg-1 HA, while there was a 4.45%-8.13% decrease and a 14.20%-46.37% increase in leaf Cd concentration in the yellow and cinnamon soils when HA and FA were applied, respectively. This finding suggests that the effects of humic acids on metal availability to plants in soil were pH-dependent, with inhibitory and stimulatory effects on acidic and alkaline soils, respectively. Therefore, humic acids (HA and FA) could be used to reduce Pb and Cd accumulation in plants growing in polluted acidic soil but not in alkaline soils.


Chen S.-L.,China Tobacco Guangdong Industrial Corporation | Shen G.-L.,China Tobacco Guangdong Industrial Corporation | Zhuo H.-L.,China Tobacco Guangdong Industrial Corporation | Lu J.-H.,South China University of Technology | And 2 more authors.
Modern Food Science and Technology | Year: 2013

Polysaccharides from different algae species (Laminaria japonica, Porphyra, Sargassum fusiform, Eucheuma) were obtained by citric acid extraction. The best algae specie was determined by analysis of the extraction yield of polysaccharides and cigarette quality. The results showed that polysaccharides from Laminaria japonica could obviously improve the cigarette quality, especially sample I and IV. By the sensory evaluation of the cigarette, the best dosage of polysaccharides was determined as 0.1%. Moreover, sample Iand IV had some promotion effect on the smoothness, irritation and finish of smoking, but showed little effect on miscellaneous gases.

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