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Patent
China Tobacco Guangdong Industrial Co. | Date: 2011-12-12

Disclosed is a method for determining pectin content in a plant sample, comprising the following steps: 1) adding an acidic alcohol solution to the plant sample, then heating the resulting mixture to reflux in a water bath, followed by a first filtration; 2) soaking the filtered residue obtained from the first filtration with an acidic solution, then heating the resulting mixture to reflux in a water bath, followed by a second filtration, then bringing to volume after cooling, obtaining filtrate for later use; 3) adding an acetic acid/sodium acetate buffer solution to treat the filtered residue obtained from the second filtration, then adding a pectinase solution and heating the resulting mixture under vibration in a water bath, followed by a third filtration to obtain a filtrate for later use; 4) sequentially adding an acetic acid/sodium acetate buffer solution and a pectinase solution to the filtrate obtained in step 2) and heating the resulting mixture under vibration in a water bath to obtain an enzymatic hydrolysate, then adding the filtrate obtained in step 3) to the enzymatic hydrolysate followed by bringing to volume, obtaining a test solution; and 5) drawing the test solution into a continuous flow analyzer to perform analysis. The method disclosed has the advantage of providing accurate analysis results.


Trademark
China Tobacco Guangdong Industrial Co. | Date: 2016-06-14

Cigarettes; Cigarettes containing tobacco substitutes not for medical purposes; Tobacco.


Huang Y.,China Tobacco Guangdong Industrial Co. | Hu J.,China Tobacco Guangdong Industrial Co.
Chinese Journal of Chromatography (Se Pu) | Year: 2010

A method for simultaneous analysis of 20 free amino acids in tobacco was developed using liquid chromatography-electrospray ionization/ion trap tandem mass spectrometry (LC-ESI-IT-MS/MS). Tobacco samples were extracted and filtered, and analyzed without derivatization or solid phase extraction. The LC separation was carried on a reversed-phase HyPURITY C l8 column (200 mm × 2. 1 mm, 5 μm) with the mobile phase of 1% (v/v) acetonitrile/water solution containing 0. 1% nonafluoropentanoic acid and 90% acetonitrile/water solution containing 0. 1% nonafluoropentanoic acid in gradient mode. The results showed that the limits of detection (LODS) of 20 amino acids were in the range of 0. 01-0. 05 μmol/L (S/N = 3), the linear correlation coefficients were above 0. 997 7 in all cases, and the relative standard deviations (RSDs) of peak areas of the extracted ion chromatograms were in the range of 0. 78%-4. 93%. The developed method is of good efficieney, sensitivity and selectivity, and has been success-fully applied to determine the free amino acids in varieties of tobacco samples. Source


Gao W.,South China University of Technology | Chen K.,South China University of Technology | Xiang Z.,North Carolina State University | Yang F.,South China University of Technology | And 5 more authors.
Industrial Crops and Products | Year: 2013

Tobacco is a very abundant bio-based resource in nature, and large amounts of tobacco residues are produced from the cigarette industry, which can be exploited and utilized for energy. In this work, the pyrolysis of tobacco residues including stems and leaves was analyzed by a Thermogravimetric Analysis (TGA) instrument and the corresponding derivative thermogravimetric analysis (DTG). The TGA curves were obtained at the heating rate of 10. °C/min and with temperature range from 25. °C to 700. °C. The results indicated that the pyrolysis of tobacco stems and tobacco leaves included three stages: dehydration, main devolatilization, and continuous devolatilization. More importantly, between the temperature range of 200. °C and 350. °C, by using the Coats-Redfern equation, correlation coefficients of different reaction models describing the pyrolysis of tobacco stems and leaves were predicted, and the best-fit models for different stages of pyrolysis were found. The activation energies as well as frequency factors (A) from the best-fit models were calculated. © 2012 Elsevier B.V.. Source


The present invention discloses a method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco by ion chromatography The retention behavior of inorganic anions and organic acids on the anion exchange column was investigated using potassium hydroxide produced by EGC-II KOH eluent autogenerator as eluent. The optimized gradient elution condition was obtained. The samples were prepared through extraction, filtration and dilution before analysis. The separation was performed on an anion exchange column. The time of the gradient elution program was 50 mins. Under the optimized conditions, the calibration of peak area for all the analytes were linear in the ranges of 10

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