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Tan X.,The Key Laboratory of Non Wood Forest Product of Forestry Ministry | Tan X.,Central South University of forestry and Technology | Tan X.,China The National Biotechnology Laboratory oflCamellia oleifera Science Center | Chen H.,The Key Laboratory of Non Wood Forest Product of Forestry Ministry | And 9 more authors.
Advanced Science Letters | Year: 2012

In the process of monolignol biosynthesis,phenylalanine ammonialyase (PAL) is thought to be the first step towards monolignol biosynthesis and other phenolic secondary metabolisms derived from primary metabolism, and plays multiple important roles involved many biosynthesis processes of flavone, lignin and alkaloid to defense diseases and stress. Tea-oil, extracted from seeds of Camellia oleifera which only grows in South of China, has been famous all over the world for its nutrient constituents including oleic acid, linoleic acid and other unsaturated fatty acids. However, the fatty acid biosynthesis could be achieved successfully in Camellia oleifera seeds though the trees grow in the environment of diseases and insect damage, high temperature and drought with the contribution of lax on leaf surface and saponin in the seed out shell. The analysis of the components of the out shell of the seeds suggested that the content of saponin was up to 34.9%. In order to reveal the relationship between fatty acid and saponin biosynthesis stimulated by the stress of outside growth environment, a novel PAL gene was obtained and named as Co-PAL in the research. Bioinformatics analysis showed that the full-length of Co-PAL cDNA was 2426 bp in length, including a 2118 bp ORF encoding 706 amino acids, a 159 bp 5′ UTR and 174 bp 3′ UTR with a polyA tail. Multiple sequences alignment displayed that the deduced protein shared 8% similarity with defined PAL from Camellia sinensis And the protein characters performed by Antheprot 5.0 showed the pI was 6.115, it could be dissolved in water for its hydrophilicity, the molecular weight was about 77.07 kD, a potential cleavage of signal peptide was found at Ala 281, and three transmembrane regions distributed at N-terminal. In addition, many sites essential for PAL activities conserved indicated Co-PAL was a new member of PAL family which was also found in different plant species. © 2012 American Scientific Publishers. All rights reserved.

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