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Jia B.G.,Central South University of forestry and Technology | Lin Q.,Central South University of forestry and Technology | Feng Y.Z.,China Paulownia Research Center | Hu X.Y.,Anhui Agricultural University | And 3 more authors.
Genetics and Molecular Research | Year: 2015

Camellia oleifera is an important edible oil woody plant in China. Lack of useful molecular markers hinders current genetic research on this tree species. Transcriptome sequencing of developing C. oleifera seeds generated 69,798 unigenes. A total of 6949 putative microsatellites were discovered among 6042 SSR-containing unigenes. Then, 150 simple sequence repeats (SSRs) were evaluated in 20 varieties of C. oleifera. Of these, 52 SSRs revealed polymorphism, with the number of alleles per locus ranging from 2 to 15 and expected heterozygosity values from 0.269 to 0.888. The polymorphic information content varied from 0.32 to 0.897. Cross-species transferability rates in Camellia chekangoleosa and Camellia japonica were 90.4 and 78.8%, respectively. The 52 polymorphic unigene-derived SSR markers serve to enrich existing microsatellite marker resources for C. oleifera and offer potential for applications in genetic diversity evaluation, molecular fingerprinting, and genetic mapping in C. oleifera, C. chekangoleosa, and C. japonica. ©FUNPEC-RP.

Zhang L.,Central South University of forestry and Technology | Lin Q.,Central South University of forestry and Technology | Feng Y.,China Paulownia Research Center | Fan X.,Central South University of forestry and Technology | And 5 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

The Chinese chestnut (Castanea mollissima) seed provides a rich source of carbohydrates as food and feed. However, little is known about starch biosynthesis in the seeds. The objectives of this study were to determine seed composition profiles and identify genes involved in starch and sucrose metabolism. Metabolite analysis showed that starch was the major component and rapidly accumulated during seed endosperm development. Amylopectin was approximately 3-fold of amylose content in chestnut starch. Illumina platform-based transcriptome sequencing generated 56671 unigenes in two cDNA libraries from seed endosperms collected at 45 and 75 days after fl owering (DAF). A total of 1537 unigenes showed expression differences ≥2-fold in the two stages of seeds including 570 up-regulated and 967 down-regulated unigenes. One hundred and fifty-two unigenes were identified as involved in starch and sucrose metabolism, including 1 for glycogenin glucosyltransferase, 4 for adenylate transporter (brittle1-type), 3 for ADP-glucose pyrophosphorylase (AGP, not brittle2- or shrunken2-type), 3 for starch synthase (SS), 2 for starch branching enzyme, 5 for starch debranching enzyme, 11 for sucrose synthase, and 3 for sucrose-phosphate synthase. Among them, 58 unigenes showed a ≥2-fold expression difference between the 45 and 75 DAF seeds including 11 up- and 47 down-regulated unigenes. The expression of 21 unigenes putatively coding for major enzymes in starch and sucrose metabolism was validated by qPCR using RNA from five seed stages. Expression profiles and correlation analysis indicated that the mRNA levels of AGP (large and small subunits), granule-bound SS2, and soluble SS1 and SS4 were well-correlated with starch accumulation in the seeds. This study suggests that the starch biosynthesis pathway in Chinese chestnut is similar to that of potato tuber/Arabidopsis leaf and differs from that of maize endosperm. The information provides valuable metabolite and genetic resources for future research in starch and sucrose metabolism in Chinese chestnut tree. © 2014 American Chemical Society.

Zhu J.-L.,China Paulownia Research Center | Zhu J.-L.,The Eucommia Engineering Research Center | Li F.-D.,China Paulownia Research Center | Li F.-D.,The Eucommia Engineering Research Center | And 2 more authors.
Forest Research | Year: 2014

In order to reveal the formation reasons for different colors of ornamental Eucommia leaf, the leaves of Eucommia ulmoides 'Hongye', E. ulmoides 'Miye' andE. ulmoides 'Xiaoye' were studied by using freehand section combined with photosynthetic pigments and anthocyanin content measurement. The results showed that forE. ulmoides 'Hongye', the front of the blade was dark red while the back was green, the pigment causing redness concentrates on the leaf palisade tissue; among the three varieties, Ca was 1.100 3~1.314 8 mg·g-1, Cb was 0.435 4~0.467 0 mg·g-1, CCar was 0.384 7~0.445 5 mg·g-1, CT was 1.462 5~1.781 8 mg·g-1, CF was 0.140 8~0.304 2 mg·g-1, CCar and CF of E. ulmoides 'Hongye' were significantly higher than that of E. ulmoides 'Miye' and E. ulmoides 'Xiaoye', while CT/CCar was significantly lower than E. ulmoides 'Miye' and E. ulmoides 'Xiaoye', moreover, the Ca, Cb, Ca/Cb and CT showed no significant difference between E. ulmoides.'Hongye' and E. ulmoides 'Miye'. The conclusion in this study stated that the anthocyanin and carotenoids contents were higher, which was the main reason for forming red color of E. ulmoides 'Hongye'

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