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Peng H.,Southwest University | Zhao C.,Southwest University | Zhao X.,Southwest University | Chen D.,China National Tobacco Corporation | Sun X.,Southwest University
Journal of Phytopathology | Year: 2015

The virus in naturally infected, stunted Chinese mallow plants and mosaic leaves was identified as Cucumber mosaic virus (CMV). Six symptomatic plants and one symptomless plant were collected in Chongqing, China. DAS-ELISA suggested CMV was likely associated with the diseased Chinese mallow. Double-stranded RNA was extracted from the samples, analysed by RT-PCR, and the coding sequences of their coat proteins (CPs) were sequenced. The results further confirmed CMV was the pathogen causing Chinese mallow stunted, mosaic disease. The isolate was named CMV-DXC. The full sequence of CMV-DXC CP was determined, and it had the highest nucleotide identity (99.4%) of those of CMV-lily, CMV-WSJ and CMV-Hnt, respectively. Phylogenetic analysis shows that CMV-DXC belongs to CMV subgroup II. To our knowledge, this is the first report of CMV infecting Chinese mallow in China. © 2015 Blackwell Verlag GmbH.

Huang J.,Chongqing University | Wu J.,Chongqing University | Li C.,China National Tobacco Corporation | Xiao C.,Southwest University | Wang G.,Chongqing University
Journal of Phytopathology | Year: 2010

Phytophthora nicotianae is one of the most important soil-borne plant pathogens. A rapid, specific and sensitive real-time polymerase chain reaction (PCR) detection method for P. nicotianae was established, which used primers targeting the internal transcribed spacer (ITS) regions of rDNA genes of Phytophthora spp. Based on the nucleotide sequences of ITS2 of 15 different species of Phytophthora, the primers and probe were designed specifically to amplify DNA from P. nicotianae. With a series of 10-fold DNA dilutions extracted from P. nicotianae pure cultures, the detection limit was 10 pg/μl in conventional PCR, whereas in SYBR Green I PCR the detection limit was 0.12 fg/μl and in TaqMan PCR 1.2 fg/μl, and real-time PCR was 10 4-105 times more sensitive than conventional PCR. The simple and rapid procedures maximized the yield and quality of recovered DNA from soil and allowed the processing of many samples in a short time. The direct DNA extractions from soil were utilized to yield DNA suitable for PCR. By combining this protocol with the real-time PCR procedure it has been possible to specifically detect P. nicotianae in soil, and the degree of sensitivity was 1.0 pg/μl. The system was applied to survey soil samples from tobacco field sites in China for the presence of P. nicotianae and the analyses of naturally infested soil showed the reliability of the real-time PCR method. © 2009 Chongqing University.

Yao Z.,China Tobacco Henan Industrial Co. | Fan W.,China Tobacco Henan Industrial Co. | Xiao Y.,China Tobacco Henan Industrial Co. | Zhang S.,China Tobacco Henan Industrial Co. | Wang D.,China National Tobacco Corporation
Tobacco Science and Technology | Year: 2015

The original temperature control system in sink-type stem conditioner was affected by the fluctuation of water level greatly, which resulted in the fluctuation of water temperature and further the temperature of and moisture content in output stem. By analyzing the reasons causing the fluctuation of water temperature, the water supply, drain and heating pipelines were improved, instead of limit control, PID closed loop control was used for controlling water level and temperature. The results showed that the periodic fluctuation of water level was avoided and the water temperature became stabler. A comparative test of water temperature of 60℃ indicated that the temperature difference reduced from ±5.52℃ to ±1.43℃ and the standard deviation from 1.02℃ to 0.62℃. The precise control of water temperature ensures the stability of temperature and moisture content of output stem and fulfills the technical requirements of cut stem production. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

Pang S.,Sichuan Agricultural University | Pang S.,China National Tobacco Corporation | Li T.-X.,Sichuan Agricultural University | Zhang X.-F.,Peking University | And 2 more authors.
Geoderma | Year: 2011

Spatial distribution of soil lead (Pb) in cropland is an important reference for regional planning and eco-environmental protection, but related information was not available in an individual county with potential contamination in the high-speed developing Chengdu Plain. In this investigation, 623 topsoil samples, randomly taken from the Shuangliu county, Sichuan province, China, were analyzed for the spatial variability of soil Pb and associated influencing factors by using multiple methods. The results showed that soil Pb presented a low spatial correlation in the isotropic scope, whereas relatively strong spatial correlation was observed in the directions of N65°E and N80°E. High risk areas with great probability of the soil Pb concentrations exceeding the baseline values (50 mg/kg and 80 mg/kg) were estimated to dispersely distribute across the study area. Shortest significant range (SSR) tests indicated that the soil Pb concentrations in plain regions and hilly regions were significantly higher than those in the mountainous regions. Furthermore, soils developed from the gray alluvium and old alluvium demonstrated significantly higher Pb concentrations than those from the purplish rock saprolith. Soil Pb concentrations in the buffer zones (0-800 m) of the four main highways firstly increased and then decreased with the increase in distance from these highways. The application rate of fertilizers, pesticides, and animal husbandry income was found to be responsible for the spatial variation of soil Pb. These results will be of reference values in environmental management and decision making in the agricultural sector. © 2011 Elsevier B.V.

Zhan Z.,Wuhan University | Liu X.,Wuhan University | Li H.,Wuhan University | Fan M.,China National Tobacco Corporation | Huang C.,Wuhan University
Materials Letters | Year: 2016

V2O3 nanoparticles, with belt-like morphology, have been successfully synthesized by a facile hydrothermal route and subsequent calcination. It was found that the ratio of H2C2O4 plays a duel role in the formation of the precursor VO2(B) through the hydrothermal process and in the morphology of the annealed V2O3 nanoparticles. Furthermore, the phase transition properties of V2O3 nanobelts were studied by the differential scanning calorimetry (DSC) curve, which shows that the phase transition temperature (Tc) of the samples is higher and it could be changed accurately by the doping of Cr, indicating that the as-obtained V2O3 nanobelts had potential applications in electrochemical catalysts and conductive composite polymeric materials. © 2015 Elsevier B.V. All rights reserved.

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