China National Research Institute of Food & Fermentation Industries

Beijing, China

China National Research Institute of Food & Fermentation Industries

Beijing, China
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Zhao R.-J.,Beijing Forestry University | Zhao R.-J.,China National Research Institute of Food & Fermentation Industries | Huo C.-Y.,Beijing Forestry University | Qian Y.,Beijing Forestry University | And 2 more authors.
Food Chemistry | Year: 2017

This study was to find an effective process to extract bioactive peptides from mushroom foot and determine their effects on activation of alcohol metabolic enzymes in vitro. The optimum extraction assisted by ultra-high-pressure processing of mushroom foot peptides was obtained with a pressure of 400 MPa and a processing time of 10 min. After ultrafiltration, peptides with molecular weight of 0–3 kDa had the highest activity to activate alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) by 70.79% and 71.35%, respectively. Following dextran gel chromatography, two peaks (p-I and p-II) appeared and the activation activities on ADH and ALDH of p-I were 72.00% and 73.43%, both higher than p-II. Nine peptides were found in p-I as determined by LC–MS/MS, and two of them (IPLH and IPIVLL) were synthesized. IPLH activated ADH and ALDH by 42.7% and 29.2% respectively, which were higher than IPIVLL. © 2017 Elsevier Ltd


Wang C.,China Agricultural University | Wang C.,China National Research Institute of Food & Fermentation Industries | Liu X.,China Agricultural University | Sun X.,China Agricultural University | And 3 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2016

Melatonin is a widely existed small active molecule in human, animal and plant with very important physiological functions. Melatonin and its isomers were found in many food styles with high level in the last several years, and it has become a new research hotspot recently. This review summarized the finding and detection methods of melatonin and its isomers, the concentration of melatonin and its isomers in different food styles and the interaction between melatonin and its isomers with microbes. Some tendencies of melatonin and its isomers in food research were presented anddiscussed, aiming to supply reference to the further research in this paper. © 2016, Editorial Office of Journal of CIFST. All right reserved.


Wang O.,China Agricultural University | Wei Y.,China National Research Institute of Food & Fermentation Industries | Tang M.,China Agricultural University | Zhou F.,China Agricultural University | Ji B.,China Agricultural University
Journal of Chinese Institute of Food Science and Technology | Year: 2015

The aim of the present study was to analyzethe chemical components of the Schisandra chinensis fruit and to compares the juice deacidification effect by calcium carbonate and anion exchange resin respectively. The results showed that Schisandra chinensis fruit was rich in anthocyanins, polyphenols, and organic acids. Chemical deacidification by calcium carbonate could remain most of the polyphenols, but calcium precipitation was easy to appear during storage. As for the physical deacidification method, five kinds of anion exchange resin were chosen for the static deacidication study. Resin D330 showed the lowest exchange rate, resin XFTS-1300 and resin Lx-500b absorbed most of the juice polyphenols, and resin Amberlite IRA 67 and resin Lewait MP 62-ENG showed the best static deacidfication effect. On this basis, the dynamic deacidficaiton effect by under resin Amberlite IRA 67 and resin Lewait MP 62-ENG were carried out at different flow velocities. Results showed that resin Lewait MP 62-ENG at 35 BV/h was the most suitable method to lower the Schisandra chinensis juice acidity. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.


PubMed | China National Research Institute of Food & Fermentation Industries and China Agricultural University
Type: Journal Article | Journal: PloS one | Year: 2015

The high morbidity of metabolic dysfunction diseases has heightened interest in seeking natural and safe compounds to maintain optimal health. -Oryzanol (OZ), the ferulic acid (FA) ester with phytosterols, mainly present in rice bran has been shown to improve markers of metabolic syndrome. This study investigates the effects of FA and OZ on alleviating high-fat and high-fructose diet (HFFD)-induced metabolic syndrome parameters.Male SD rats were fed with a regular rodent diet, HFFD, or HFFD supplemented with 0.05% FA or 0.16% OZ (equimolar concentrations) for 13 weeks. Food intake, organ indices, serum lipid profiles, glucose metabolism, insulin resistance (IR) index and cytokine levels were analyzed. The mechanisms were further investigated in oleic acid-stimulated HepG2 cells by analyzing triglyceride (TG) content and lipogenesis-related gene expressions.In the in vivo study, FA and OZ exhibited similar effects in alleviating HFFD-induced obesity, hyperlipidemia, hyperglycemia, and IR. However, only OZ treatment significantly decreased liver index and hepatic TG content, lowered serum levels of C-reactive protein and IL-6, and increased serum concentration of adiponectin. In the in vitro assay, only OZ administration significantly inhibited intracellular TG accumulation and down-regulated expression of stearoyl coenzyme-A desaturase-1, which might facilitate OZ to enhance its hepatoprotective effect.OZ is more effective than FA in inhibiting hepatic fat accumulation and inflammation. Thus, FA and OZ could be used as dietary supplements to alleviate the deleterious effects of HFFD.


PubMed | China National Research Institute of Food & Fermentation Industries, Academy of State Administration of Grain, COFCO and Jiangnan University
Type: | Journal: International journal of biological macromolecules | Year: 2014

The effect of cassava cross-linked octenyl succinic maltodextrin (CCOMD) on diabetic mice was investigated in this study. For CCOMD-L (low dose) and CCOMD-H (high dose) groups, the body weights were recovered by 14.9% and 18.5%, respectively, which were significantly higher than that of model control group. It was also found that the blood glucose and insulin levels were ameliorated in the diabetic mice by the CCOMD diet. Moreover, the CCOMD diet decreased the plasma total cholesterol level (8.1-9.1%) and LDL cholesterol level (28.9-39.4%), and improved the plasma HDL cholesterol level (13.8-15.3%) and intestine short chain fatty acid content. The results indicated that CCOMD administration may be helpful for treating and preventing hyperlipidemia and hyperglycemia in diabetes.


PubMed | China National Research Institute of Food & Fermentation Industries and Nanjing Agricultural University
Type: Comparative Study | Journal: PloS one | Year: 2014

The use of non-destructive methods to detect egg hatching properties could increase efficiency in commercial hatcheries by saving space, reducing costs, and ensuring hatching quality. For this purpose, a hyperspectral imaging system was built to detect embryo development and vitality using spectral and morphological information of hatching eggs. A total of 150 green shell eggs were used, and hyperspectral images were collected for every egg on day 0, 1, 2, 3 and 4 of incubation. After imaging, two analysis methods were developed to extract egg hatching characteristic. Firstly, hyperspectral images of samples were evaluated using Principal Component Analysis (PCA) and only one optimal band with 822 nm was selected for extracting spectral characteristics of hatching egg. Secondly, an image segmentation algorithm was applied to isolate the image morphologic characteristics of hatching egg. To investigate the applicability of spectral and image morphological analysis for detecting egg early hatching properties, Learning Vector Quantization neural network (LVQNN) was employed. The experimental results demonstrated that model using image morphological characteristics could achieve better accuracy and generalization than using spectral characteristic parameters, and the discrimination accuracy for eggs with embryo development were 97% at day 3, 100% at day 4. In addition, the recognition results for eggs with weak embryo development reached 81% at day 3, and 92% at day 4. This study suggested that image morphological analysis was a novel application of hyperspectral imaging technology to detect egg early hatching properties.


She X.,Beijing Forestry University | She X.,China National Research Institute of Food & Fermentation Industries | Wang F.,Beijing Forestry University | Ma J.,Beijing Forestry University | And 3 more authors.
Food and Agricultural Immunology | Year: 2016

To assess the effect of corn peptides (CPs) as an antioxidant on ethanol (EtOH)-induced oxidative stress in HepG2 cells, the cells were preincubated with CPs for 4 h before treated with EtOH. Methyl thiazolyl tetrazolium and flow cytometry assay were performed to detect the viability of CP-treated HepG2 cells and control cells. The activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by corresponding kits, respectively. The results showed that treatment with 300 mmol/L EtOH for 24 h resulted in a death of around 50% of the HepG2 cells. Pretreatments of appropriate concentrations of CPs (more than 5 mg/mL, particularly at 20 mg/mL) significantly prevented EtOH-induced cytotoxicity and reduced the increase of ALT, AST, LDH and MDA contents, as well as inhibited the reduction of SOD contents in the HepG2 cells, indicating that CPs protected the cells from oxidative damage induced by EtOH metabolism, and thus had a great application potential in hepatoprotective nutraceuticals. © 2015 Taylor & Francis.


Luan C.,China National Research Institute of Food & Fermentation Industries | Miao H.,Chinese National Human Genome Center | Miao H.,SinoGenoMax Co. | Zhu B.,CAS Institute of Microbiology
Gut Microbes | Year: 2015

Fungal microbiota (mycobiota) is potentially involved in the intestinal illness. The characterization of the mycobiota in patients with adenomas is essential for understanding the etiology of the pre-cancerous lesion since the mycobiota is potentially associated with the presents of adenomas. The recovery of the mycobiome may also help to identify the potential biomarkers which may closely relate to different stages of adenoma © 2015, Taylor & Francis Group, LLC.


Cheng Z.,Beijing Forestry University | Cheng Z.,China National Research Institute of Food & Fermentation Industries | Yang Y.,Beijing Forestry University | Kuang Q.,Beijing Forestry University | And 3 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2015

The research aimed to optimize the extraction method and detect the alcohol metabolism activity in vitro of Lentinus edodes peptides, further to explore its nutritional function. Peptide was obtained from different parts of Lentinus edodes (umbrella, foot, and whole body) with four proteases (Papain, Alcalase, Parenzyme, and Protamex), respectively. The degree of hydrolysis (DH) and thel, 1-diphenyl-2-picrylhydrazyl (DPPH·) scavenging activity of Lentinus edodes peptide was investigated to determine the optimum enzymatic hydrolysis procedure, which was proved to be ideal combination of Protamex and Lentinus edodes foot powders. The obtained hydrolysates were further separated by ultrafiltration to investigate the antioxidant activity and the effects on activating alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in vitro. The results showed that the root powder hydrolysates had a relatively lower antioxidant activity than that whole body powder, while the root hydrolysates by Protamex possessed the highest DPPH radical scavenging capability. The optimum enzymatic hydrolysis procedure of Lentinus edodes foots was as follows: pH value 6.0, hydrolyzing time 3 h, temperature 50℃, and the ratio of enzyme to substrate 0.2%. The amino content of the obtained Lentinus edodes peptides was up to 29.23% in this procedure. The study revealed that the peptides 5-10 ku obtained from Lentinus edodes foot had activities of facilitating alcohol metabolism activity in vitro, with activities of activating ADH (94.58%±0.74% with at 30 mg/mL) and ALDH (70.69%±1.25% at 180 mg/mL), as well as inhibiting ·OH (with an IC50=0.190 mg/mL) and scavenging DPPHo (with an IC50=0.152 mg/mL). Our study suggested that Lentinus edodes peptides would be of great potential in the application of facilitating alcohol metabolism. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.


Wang F.,Beijing Forestry University | Wang F.,China National Research Institute of Food & Fermentation Industries | Miao M.,Beijing Forestry University | Chen B.,Beijing Forestry University | And 4 more authors.
Food and Agricultural Immunology | Year: 2015

Spirulina platensis (SP) is well recognised for its abundant unsaturated fatty acids, especially γ-linolenic acid (GLA). In this study, GLA was extracted from SP. Antineoplastic and antithrombotic activities of GLA were evaluated by measuring the concentrations of reactive oxygen species (ROS) and malondialdehyde, as well as inducing apoptosis in HepG2 human hepatoma cells and diphosphatase-induced platelet aggregation inhibition assay, respectively. The study suggests that the GLA extraction from SP using ethanol achieved a higher extraction yield of 8.3 g kg−1 ± 0.17 g kg−1 (GLA/dry biomass) and inhibited cell proliferation in a dose- and time-dependent manner. GLA extract (250 µM) strongly induced ROS generation and lipid peroxidation as well as apoptosis. The extract also caused a concentration-dependent antithrombotic effect on platelet aggregation in vitro. Thus, the GLA extract from SP can be applied in health care and functional foods. © 2013, Taylor & Francis.

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