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Luan C.,China National Research Institute of Food & Fermentation Industries | Miao H.,Chinese National Human Genome Center | Zhu B.,CAS Institute of Microbiology
Gut Microbes | Year: 2015

Fungal microbiota (mycobiota) is potentially involved in the intestinal illness. The characterization of the mycobiota in patients with adenomas is essential for understanding the etiology of the pre-cancerous lesion since the mycobiota is potentially associated with the presents of adenomas. The recovery of the mycobiome may also help to identify the potential biomarkers which may closely relate to different stages of adenoma © 2015, Taylor & Francis Group, LLC.

Wang O.,China Agricultural University | Wei Y.,China National Research Institute of Food & Fermentation Industries | Tang M.,China Agricultural University | Zhou F.,China Agricultural University | Ji B.,China Agricultural University
Journal of Chinese Institute of Food Science and Technology | Year: 2015

The aim of the present study was to analyzethe chemical components of the Schisandra chinensis fruit and to compares the juice deacidification effect by calcium carbonate and anion exchange resin respectively. The results showed that Schisandra chinensis fruit was rich in anthocyanins, polyphenols, and organic acids. Chemical deacidification by calcium carbonate could remain most of the polyphenols, but calcium precipitation was easy to appear during storage. As for the physical deacidification method, five kinds of anion exchange resin were chosen for the static deacidication study. Resin D330 showed the lowest exchange rate, resin XFTS-1300 and resin Lx-500b absorbed most of the juice polyphenols, and resin Amberlite IRA 67 and resin Lewait MP 62-ENG showed the best static deacidfication effect. On this basis, the dynamic deacidficaiton effect by under resin Amberlite IRA 67 and resin Lewait MP 62-ENG were carried out at different flow velocities. Results showed that resin Lewait MP 62-ENG at 35 BV/h was the most suitable method to lower the Schisandra chinensis juice acidity. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.

She X.,Beijing Forestry University | She X.,China National Research Institute of Food & Fermentation Industries | Wang F.,Beijing Forestry University | Ma J.,Beijing Forestry University | And 3 more authors.
Food and Agricultural Immunology | Year: 2016

To assess the effect of corn peptides (CPs) as an antioxidant on ethanol (EtOH)-induced oxidative stress in HepG2 cells, the cells were preincubated with CPs for 4 h before treated with EtOH. Methyl thiazolyl tetrazolium and flow cytometry assay were performed to detect the viability of CP-treated HepG2 cells and control cells. The activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by corresponding kits, respectively. The results showed that treatment with 300 mmol/L EtOH for 24 h resulted in a death of around 50% of the HepG2 cells. Pretreatments of appropriate concentrations of CPs (more than 5 mg/mL, particularly at 20 mg/mL) significantly prevented EtOH-induced cytotoxicity and reduced the increase of ALT, AST, LDH and MDA contents, as well as inhibited the reduction of SOD contents in the HepG2 cells, indicating that CPs protected the cells from oxidative damage induced by EtOH metabolism, and thus had a great application potential in hepatoprotective nutraceuticals. © 2015 Taylor & Francis.

Cheng Z.,Beijing Forestry University | Cheng Z.,China National Research Institute of Food & Fermentation Industries | Yang Y.,Beijing Forestry University | Kuang Q.,Beijing Forestry University | And 3 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2015

The research aimed to optimize the extraction method and detect the alcohol metabolism activity in vitro of Lentinus edodes peptides, further to explore its nutritional function. Peptide was obtained from different parts of Lentinus edodes (umbrella, foot, and whole body) with four proteases (Papain, Alcalase, Parenzyme, and Protamex), respectively. The degree of hydrolysis (DH) and thel, 1-diphenyl-2-picrylhydrazyl (DPPH·) scavenging activity of Lentinus edodes peptide was investigated to determine the optimum enzymatic hydrolysis procedure, which was proved to be ideal combination of Protamex and Lentinus edodes foot powders. The obtained hydrolysates were further separated by ultrafiltration to investigate the antioxidant activity and the effects on activating alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in vitro. The results showed that the root powder hydrolysates had a relatively lower antioxidant activity than that whole body powder, while the root hydrolysates by Protamex possessed the highest DPPH radical scavenging capability. The optimum enzymatic hydrolysis procedure of Lentinus edodes foots was as follows: pH value 6.0, hydrolyzing time 3 h, temperature 50℃, and the ratio of enzyme to substrate 0.2%. The amino content of the obtained Lentinus edodes peptides was up to 29.23% in this procedure. The study revealed that the peptides 5-10 ku obtained from Lentinus edodes foot had activities of facilitating alcohol metabolism activity in vitro, with activities of activating ADH (94.58%±0.74% with at 30 mg/mL) and ALDH (70.69%±1.25% at 180 mg/mL), as well as inhibiting ·OH (with an IC50=0.190 mg/mL) and scavenging DPPHo (with an IC50=0.152 mg/mL). Our study suggested that Lentinus edodes peptides would be of great potential in the application of facilitating alcohol metabolism. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.

Wang F.,Beijing Forestry University | Wang F.,China National Research Institute of Food & Fermentation Industries | Miao M.,Beijing Forestry University | Chen B.,Beijing Forestry University | And 4 more authors.
Food and Agricultural Immunology | Year: 2015

Spirulina platensis (SP) is well recognised for its abundant unsaturated fatty acids, especially γ-linolenic acid (GLA). In this study, GLA was extracted from SP. Antineoplastic and antithrombotic activities of GLA were evaluated by measuring the concentrations of reactive oxygen species (ROS) and malondialdehyde, as well as inducing apoptosis in HepG2 human hepatoma cells and diphosphatase-induced platelet aggregation inhibition assay, respectively. The study suggests that the GLA extraction from SP using ethanol achieved a higher extraction yield of 8.3 g kg−1 ± 0.17 g kg−1 (GLA/dry biomass) and inhibited cell proliferation in a dose- and time-dependent manner. GLA extract (250 µM) strongly induced ROS generation and lipid peroxidation as well as apoptosis. The extract also caused a concentration-dependent antithrombotic effect on platelet aggregation in vitro. Thus, the GLA extract from SP can be applied in health care and functional foods. © 2013, Taylor & Francis.

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