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Lu X.,Henan Agricultural University | Song Z.,Henan Agricultural University | Huang Y.,Henan Agricultural University | Huang Y.,Henan Provincial Tobacco Company | And 6 more authors.
Tobacco Science and Technology | Year: 2016

In order to reduce the cost of tobacco flue-curing, an 8-tunnel 30 m continuous flue-curing barn was developed on the basis of stepping flue-curing barn. The developed barn was composed of tobacco loading, preparing, flue-curing, cooling and tobacco unloading sections. The flue-curing section was divided into 6 sub-sections dependent on curing requirements, and the temperature and relative humidity in each sub-section were separately set and monitored during curing. The curing results were compared with conventional bulk barn. The results showed that the running time of continuous flue-curing barn was in the range of 134-146 h, and the variations of temperature, relative humidity and air velocity in the barn met the curing requirements. Comparing with bulk curing barn, the fresh/dry tobacco weight ratio decreased slightly, the proportion of orange leaves significantly increased; the average prices of middle leaves and upper leaves raised by 1.36 RMB yuan/kg (6.04%) and 1.13 RMB yuan/kg (5.08%), the curing efficiencies promoted by 23.1 and 25.8 kg/day per worker, the energy consumption reduced by 0.18 and 0.07 RMB yuan/kg dry leaves, respectively. Continuous flue-curing barn achieves efficient heat utilization and satisfies the requirements in each curing stage. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Huang Z.,China Tobacco Hunan Industrial Co. | Zhang Q.,China Tobacco Hunan Industrial Co. | Zhang Q.,Xiangxi Hesheng Tobacco Development Co. | Cui Y.,China National Leaf Tobacco Corporation | And 6 more authors.
Tobacco Science and Technology | Year: 2014

In order to detect the moisture content in tobacco bales quickly, a microwave moisture meter (MW-T) and its relevant detection system were introduced, the performance of MW-T was evaluated with method, and the calibration model of MW-T was established. The results showed that: R2 of the developed model was 0.845. The values of Cg and Cgk of MW-T were 1.76 and 1.55, respectively. The way of bale arrangement and bale density had no effect on the moisture content measurement. The average absolute difference between results of MW-T and standard oven method was 0.38% , and the absolute difference of 96.25% of all samples were within ±0.75%.


Lai R.,Fujian Agriculture and forestry University | Lai R.,Longyan Branch of Fujian Provincial Tobacco Company | Lai R.,Longyan Substation of Fujian Science and Research Institute of Tobacco Farming | Liao L.,Fujian Agriculture and forestry University | And 5 more authors.
Tobacco Science and Technology | Year: 2015

In order to know the spatial distribution of Alternaria alternata (A. alternata) and the damage it caused to flue-cured tobacco of different varieties, field investigation trials were carried out systematically, and its niche characteristics in Longyan tobacco growing areas were analyzed from the points of host niche and spatial niche. The results showed that the host niche breadth of A. alternata was quite large to the trial tobacco varieties with the incidence and disease index niche breadths of 0.918 7 and 0.931 4, respectively. The varieties in the order of resistance against A. alternata were Minyan 35>CB-1>C2>Honghuadajinyuan, which indicated that the germs basically infected all the trial varieties. Moreover, its spatial niche breadth was also large. The varieties in the order of the spatial niche breadth of A. alternata incidence were Minyan 35>C2>Honghuadajinyuan>CB-1, while in the order of the spatial niche breadth of disease index were Minyan 35>CB-1>Honghuadajinyuan>C2. It suggested that the germs could fully occupy the upper, middle and lower parts of tobacco plant and cause damage to whole plant. Knowing the distribution of A. alternata and the resistance of different varieties would be helpful to its effective control. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Li R.,China National Leaf Tobacco Corporation
Tobacco Science and Technology | Year: 2013

For solving the problems existing in loose leaf purchasing, such as high investment, short of professional graders, and lower grading skills, the experiments on a new loose leaf purchasing system involving harvest and curing by stalk position and grading by curing barn were carried out. The results showed that through introducing a harvesting license system, specialized curing and household pre-inspection and other measures, the new purchasing system not only retained the advantages of the original loose leaf grading and purchasing system, but also realized scientific harvest by stalk position, sewing green leaves after classification, cured leaves sorting (removing greenish and variegated leaves), marking and bundling leaves from the same stalk position and curing barn, and purchasing by whole barn. The results of application indicated that the new system improved grading quality, increased qualified rates, and expedited the process of leaf grading and purchasing.


Shang H.,CAS Zhengzhou Research Institute | Chen J.,China National Leaf Tobacco Corporation | Zhang Y.,CAS Zhengzhou Research Institute | Dai H.,CAS Zhengzhou Research Institute | Zhang S.,CAS Zhengzhou Research Institute
Tobacco Science and Technology | Year: 2014

Soil pot and water culture experiments of two tobacco genotypes of different Cd accumulating patter, N. tabacum (K326) and N. rustica, were carried out to study their difference in total and individual contents of low molecular weight organic acids (LMWOA) in the exudates of root system. The results showed that: 1) In soil pot experiment, tobacco seedling growth was promoted by Cd treatment at the rate of 1 mg/kg, while it was seriously inhibited at Cd rate of 10 mg/kg. N. rustica was more tolerant to Cd than was K326. 2) In soil pot and water culture experiments, LMWOAs secretion was promoted at low Cd level, it was inhibited at high Cd level. Total amount of LMWOAs exudates by N. rustica was higher than that by K326, which indicated that higher LMWOAs exudation might be one of the major factors leading to higher tolerance of N. rustica to Cd. 3) The contents of oxalic acid, tartaric acid, methanoic acid and lactic acid were higher in N. rustica than in K326, however, the proportions of the four organic acids in total organic acids were not significantly different between the two genotypes; the different tolerance to Cd of the two genotypes probably related to the amounts of the four organic acids, rather than the proportion of each organic acid.


Shang H.,CAS Zhengzhou Research Institute | Chen J.,China National Leaf Tobacco Corporation | Guo Y.,CAS Zhengzhou Research Institute | Cheng G.,Xuchang Tobacco Monopoly Bureau | And 4 more authors.
Tobacco Science and Technology | Year: 2014

In order to explore the genotype difference mechanism of Cd vacuole interception in tobacco root systems, hydroponic seedling experiments with different Cd treatments were carried out to study the differences of root system Cd chelating ability and related genes expression between two tobacco genotypes (K326 and N. rustica). The results showed that: 1) After Cd treatments, GSH decreased, while phytochelatins (PCs) increased. Comparing with the control, the contents of total non-protein thiol (NPT) and PCs in the roots of K326 and N. rustica in the treatment of 1 μmol/L Cd increased by 45.89%, 57.75% and 55.58%, 84.00%, while GSH content decreased by 10.70% and 62.09%, respectively. The change of GSH and PCs caused by Cd treatment was more significant in N.rustica than in K326. 2) Cd treatment promoted Cd in roots transforming from exchangeable state into chelated state, and promoted PCs transforming into high-molecular-weight bound state (HMW). Notably in N. rustica. 3) In the control, HMW and LMW each accounted roughly for half of the root system chelates in K326 or N. rustica. However, induced by the treatment of 1 μmol/L Cd, HMW accounted for 77.43% of root chelates in K326, and almost all Cd was in the form of HMW state in N.rustica. Under Cd inducing conditions, the transition ability of Cd to chelated state and the binding ability were significantly higher in N. rustica than in K326, it indicated that the difference of roots'Cd chelating ability was one of the reasons causing the difference of Cd accumulation between K326 and N. rustica. 4) PCS1 expression in the roots of N. rustica was highly sensitive to Cd, and increased by 257.33% in the treatment of 1 μmol/L Cd; while PCS1 expression in K326 was not affected significantly by Cd treatment. ©, 2014, Editorial Office of Tobacco Science and Technology. All right reserved.


Zhou X.,Zhengzhou University | Zhou X.,CAS Zhengzhou Research Institute | Wei C.,CAS Zhengzhou Research Institute | Huang J.,Zhengzhou University | And 10 more authors.
Tobacco Science and Technology | Year: 2014

The miRNA398 in model plants could repress the expression of Cu/Zn-SOD gene. In order to reveal the biological function of miRNA398, fluorescent quantitative PCR (qPCR) technology was employed to analyze the expression levels of miRNA398 and Cu/Zn-SOD gene in cv. CB-1 and B37 under drought stress. Meanwhile, the locus where miRNA398 combining with Cu/Zn-SOD gene was also predicted. The results showed that miRNA398 was complementary to a 21 bp sequence in 5' untranslated region of Cu/Zn-SOD gene, thereby to repress the expression of Cu/Zn-SOD gene. Under drought stress, the expression level of miRNA398 was up-regulated in CB-1 and down-regulated in B37, while that of Cu/Zn-SOD gene was on the contrary; which indicated that miRNA398 repressed the expression of Cu/Zn-SOD gene and responsed to drought stress in two different ways in the two varieties of different resistance to drought.


Bao Q.,China National Leaf Tobacco Corporation | Zhang Y.,CAS Zhengzhou Research Institute | Wang A.,CAS Zhengzhou Research Institute | Guo W.,CAS Zhengzhou Research Institute
Tobacco Science and Technology | Year: 2015

In order to improve the industrial usability of flue-cured tobacco leaves, the variation tendencies of main chemical components (contents of nicotine, total N, reduced sugar, K, Cl, starch) in middle leaf samples (C3F) from main tobacco growing areas in China during 2002-2013 were analyzed. The results showed that: 1) The content ranges of nicotine, total N, reduced sugar, K, Cl, and starch in middle leaves were 1.38%-3.77%, 1.35%-2.96%, 16.96%-32.76%, 1.09%-3.07%, 0.11%-0.79%, and 2.00%-8.07%, respectively. 2) The contents of starch, K and Cl in middle leaves were relatively stable over the years, however, reduced sugar content increased from 21.79%) in 2002 to 26.54% in 2013, and increased obviously from 2005 to 2008. 3) The average nicotine content decreased from 2.72% in 2002 to 2.29% in 2013 and obviously decreased from 2003 to 2007, and total N content changed in a way similar to that of nicotine. 4) The changes of both tobacco planting areas and cultivars did not closely correlated to the changes of chemical components. The raising of reduced sugar content was mainly attributed to its increase in the areas other than north tobacco growing areas, while the declining of the contents of nicotine and total N were mainly relevant to their decrease in the upper and middle reaches of the Yangtze River, southwest and southeast tobacco growing areas. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

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