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Li R.,China National Leaf Tobacco Corporation
Tobacco Science and Technology | Year: 2013

For solving the problems existing in loose leaf purchasing, such as high investment, short of professional graders, and lower grading skills, the experiments on a new loose leaf purchasing system involving harvest and curing by stalk position and grading by curing barn were carried out. The results showed that through introducing a harvesting license system, specialized curing and household pre-inspection and other measures, the new purchasing system not only retained the advantages of the original loose leaf grading and purchasing system, but also realized scientific harvest by stalk position, sewing green leaves after classification, cured leaves sorting (removing greenish and variegated leaves), marking and bundling leaves from the same stalk position and curing barn, and purchasing by whole barn. The results of application indicated that the new system improved grading quality, increased qualified rates, and expedited the process of leaf grading and purchasing. Source

Shang H.,CAS Zhengzhou Research Institute | Chen J.,China National Leaf Tobacco Corporation | Guo Y.,CAS Zhengzhou Research Institute | Cheng G.,Xuchang Tobacco Monopoly Bureau | And 4 more authors.
Tobacco Science and Technology | Year: 2014

In order to explore the genotype difference mechanism of Cd vacuole interception in tobacco root systems, hydroponic seedling experiments with different Cd treatments were carried out to study the differences of root system Cd chelating ability and related genes expression between two tobacco genotypes (K326 and N. rustica). The results showed that: 1) After Cd treatments, GSH decreased, while phytochelatins (PCs) increased. Comparing with the control, the contents of total non-protein thiol (NPT) and PCs in the roots of K326 and N. rustica in the treatment of 1 μmol/L Cd increased by 45.89%, 57.75% and 55.58%, 84.00%, while GSH content decreased by 10.70% and 62.09%, respectively. The change of GSH and PCs caused by Cd treatment was more significant in N.rustica than in K326. 2) Cd treatment promoted Cd in roots transforming from exchangeable state into chelated state, and promoted PCs transforming into high-molecular-weight bound state (HMW). Notably in N. rustica. 3) In the control, HMW and LMW each accounted roughly for half of the root system chelates in K326 or N. rustica. However, induced by the treatment of 1 μmol/L Cd, HMW accounted for 77.43% of root chelates in K326, and almost all Cd was in the form of HMW state in N.rustica. Under Cd inducing conditions, the transition ability of Cd to chelated state and the binding ability were significantly higher in N. rustica than in K326, it indicated that the difference of roots'Cd chelating ability was one of the reasons causing the difference of Cd accumulation between K326 and N. rustica. 4) PCS1 expression in the roots of N. rustica was highly sensitive to Cd, and increased by 257.33% in the treatment of 1 μmol/L Cd; while PCS1 expression in K326 was not affected significantly by Cd treatment. ©, 2014, Editorial Office of Tobacco Science and Technology. All right reserved. Source

Huang Z.,China Tobacco Hunan Industrial Co. | Zhang Q.,China Tobacco Hunan Industrial Co. | Zhang Q.,Xiangxi Hesheng Tobacco Development Co. | Cui Y.,China National Leaf Tobacco Corporation | And 6 more authors.
Tobacco Science and Technology | Year: 2014

In order to detect the moisture content in tobacco bales quickly, a microwave moisture meter (MW-T) and its relevant detection system were introduced, the performance of MW-T was evaluated with method, and the calibration model of MW-T was established. The results showed that: R2 of the developed model was 0.845. The values of Cg and Cgk of MW-T were 1.76 and 1.55, respectively. The way of bale arrangement and bale density had no effect on the moisture content measurement. The average absolute difference between results of MW-T and standard oven method was 0.38% , and the absolute difference of 96.25% of all samples were within ±0.75%. Source

Zhou X.,Zhengzhou University | Zhou X.,CAS Zhengzhou Research Institute | Wei C.,CAS Zhengzhou Research Institute | Huang J.,Zhengzhou University | And 10 more authors.
Tobacco Science and Technology | Year: 2014

The miRNA398 in model plants could repress the expression of Cu/Zn-SOD gene. In order to reveal the biological function of miRNA398, fluorescent quantitative PCR (qPCR) technology was employed to analyze the expression levels of miRNA398 and Cu/Zn-SOD gene in cv. CB-1 and B37 under drought stress. Meanwhile, the locus where miRNA398 combining with Cu/Zn-SOD gene was also predicted. The results showed that miRNA398 was complementary to a 21 bp sequence in 5' untranslated region of Cu/Zn-SOD gene, thereby to repress the expression of Cu/Zn-SOD gene. Under drought stress, the expression level of miRNA398 was up-regulated in CB-1 and down-regulated in B37, while that of Cu/Zn-SOD gene was on the contrary; which indicated that miRNA398 repressed the expression of Cu/Zn-SOD gene and responsed to drought stress in two different ways in the two varieties of different resistance to drought. Source

Bao Q.,China National Leaf Tobacco Corporation | Zhang Y.,CAS Zhengzhou Research Institute | Wang A.,CAS Zhengzhou Research Institute | Guo W.,CAS Zhengzhou Research Institute
Tobacco Science and Technology | Year: 2015

In order to improve the industrial usability of flue-cured tobacco leaves, the variation tendencies of main chemical components (contents of nicotine, total N, reduced sugar, K, Cl, starch) in middle leaf samples (C3F) from main tobacco growing areas in China during 2002-2013 were analyzed. The results showed that: 1) The content ranges of nicotine, total N, reduced sugar, K, Cl, and starch in middle leaves were 1.38%-3.77%, 1.35%-2.96%, 16.96%-32.76%, 1.09%-3.07%, 0.11%-0.79%, and 2.00%-8.07%, respectively. 2) The contents of starch, K and Cl in middle leaves were relatively stable over the years, however, reduced sugar content increased from 21.79%) in 2002 to 26.54% in 2013, and increased obviously from 2005 to 2008. 3) The average nicotine content decreased from 2.72% in 2002 to 2.29% in 2013 and obviously decreased from 2003 to 2007, and total N content changed in a way similar to that of nicotine. 4) The changes of both tobacco planting areas and cultivars did not closely correlated to the changes of chemical components. The raising of reduced sugar content was mainly attributed to its increase in the areas other than north tobacco growing areas, while the declining of the contents of nicotine and total N were mainly relevant to their decrease in the upper and middle reaches of the Yangtze River, southwest and southeast tobacco growing areas. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved. Source

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