China National Hybrid Rice Research and Development Center

Changsha, China

China National Hybrid Rice Research and Development Center

Changsha, China

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Xie F.,International Rice Research Institute | Guo L.,China National Rice Research Institute | Ren G.,Sichuan Academy of Agricultural science | Hu P.,China National Rice Research Institute | And 5 more authors.
Plant Genetic Resources: Characterisation and Utilisation | Year: 2012

Investigation of genetic diversity and the relationships among varieties and breeding lines is of great importance to facilitate parental selection in the development of inbred and hybrid rice varieties and in the construction of heterotic groups. The technology of single nucleotide polymorphism (SNP) is being advanced for the assessment of population diversity and genetic structures. We characterized 215 widely cultivated indica rice varieties developed in southern China and at the International Rice Research Institute (IRRI) using IRRI-developed SNP oligonucleotide pooled assay (OPA) to provide grouping information of rice mega-varieties for further heterotic pool study. The results revealed that the Chinese varieties were more divergent than the IRRI varieties. Two major subpopulations were clustered for the varieties using a model-based grouping method. The IRRI varieties were closely grouped and separated clearly from the majority of the Chinese varieties. The Chinese varieties were subclustered into three subgroups, but there was no clear evidence to separate the Chinese varieties into subgroups geographically, indicating a great degree of genetic integration of alleles and shared ancestries among those high-yielding modern varieties. © NIAB 2012.


Ma G.-H.,China National Hybrid Rice Research and Development Center | Yuan L.-P.,China National Hybrid Rice Research and Development Center
Journal of Integrative Agriculture | Year: 2015

This article reviews the history and progress of hybrid rice development. Hybrid rice research was initiated back in 1964, and commercialized in 1976. Three-line and two-line system hybrid rice were developed in 1974 and 1995, respectively. Research on super hybrid rice, which was first launched by Ministry of Agriculture, China in 1996, is discussed, and the great progress of super hybrid rice had been achieved with a new yield record by 15.4 t ha-1 in the 6.84 ha demonstration location in Xupu, Hunan Province, China in 2014. And the mechanism of heterosis, the techniques of hybrid seed production and the modern field managements in hybrid rice over the past decades are also discussed. Additionally, this article dealt with the intellectual property protection (IPR) and development of hybrid rice seed industry in China. Major factors that constrain hybrid rice development are analyzed and possible solutions to this problems are proposed. Finally, the authors present methods to further increase production yield, and propose an improvement for breeding super high-yielding hybrid rice based on these methods. © 2015 Chinese Academy of Agricultural Sciences.


Wang H.,Chinese University of Hong Kong | Chen J.,Chinese University of Hong Kong | Lin H.,Chinese University of Hong Kong | Yuan D.,China National Hybrid Rice Research and Development Center
Remote Sensing Letters | Year: 2010

The objective of this study is to validate the effectiveness of hyperspectral data and select hyperspectral variables to indicate differences between the non-transgenic parent and the progenies of transgenic rice regardless of gene expression. Statistically significant differences were observed in the range 510-735 nm. More differences could be observed from first-order derivative than from the original reflectance spectra. Spectral position- and index-based variables were calculated to find out the potential indicators. Both the maximum value of first-order derivative and the sum of first-order derivative in the ranges 490-530 and 560-650 nm, photochemical reflectance index (PRI) and modified chlorophyll absorption ratio index (MCARI) were different between parent group and progeny groups of transgenic rice.


Li L.,Hunan Agricultural University | Li L.,Hubei University of Education | Lu K.,Huazhong Agricultural University | Chen Z.,Hubei University of Education | And 3 more authors.
Molecular Genetics and Genomics | Year: 2010

To understand the gene activities controlling nine important agronomic quantitative traits in rice, we applied a North Carolina design 3 (NC III design) analysis to recombinant inbred lines (RILs) in highly heterotic inter- (IJ) and intra-subspecific (II) hybrids by performing the following tasks: (1) investigating the relative contribution of additive, dominant, and epistatic effects for performance traits by generation means analysis and variance component estimates; (2) detecting the number, genomic positions, and genetic effects of QTL for phenotypic traits; and (3) characterizing their mode of gene action. Under an F∞-metric, generation means analysis and variance components estimates revealed that epistatic effects prevailed for the majority of traits in the two hybrids. QTL analysis identified 48 and 66 main-effect QTL (M-QTL) for nine traits in IJ and II hybrids, respectively. In IJ hybrids, 20 QTL (41.7%) showed an additive effect of gene actions, 20 (41.7%) showed partial-to-complete dominance, and 8 (16.7%) showed overdominance. In II hybrids, 34 QTL (51.5%) exhibited additive effects, 14 (21.2%) partial-to-complete dominance, and 18 (27.3%) overdominance. There were 153 digenic interactions (E-QTL) in the IJ hybrid and 252 in the II hybrid. These results suggest that additive effects, dominance, overdominance, and particularly epistasis attribute to the genetic basis of the expression of traits in the two hybrids. Additionally, we determined that the genetic causes of phenotypic traits and their heterosis are different. In the plants we studied, the phenotypic traits investigated and their heterosis were conditioned by different M-QTL and E-QTL, respectively, and were mainly due to non-allelic interactions (epistasis). © 2010 Springer-Verlag.


Hu B.,CAS Institute of Genetics and Developmental Biology | Wang W.,CAS Institute of Genetics and Developmental Biology | Wang W.,University of Chinese Academy of Sciences | Ou S.,CAS Institute of Genetics and Developmental Biology | And 21 more authors.
Nature Genetics | Year: 2015

Asian cultivated rice (Oryza sativa L.) consists of two main subspecies, indica and japonica. Indica has higher nitrate-absorption activity than japonica, but the molecular mechanisms underlying that activity remain elusive. Here we show that variation in a nitrate-transporter gene, NRT1.1B (OsNPF6.5), may contribute to this divergence in nitrate use. Phylogenetic analysis revealed that NRT1.1B diverges between indica and japonica. NRT1.1B-indica variation was associated with enhanced nitrate uptake and root-to-shoot transport and upregulated expression of nitrate-responsive genes. The selection signature of NRT1.1B-indica suggests that nitrate-use divergence occurred during rice domestication. Notably, field tests with near-isogenic and transgenic lines confirmed that the japonica variety carrying the NRT1.1B-indica allele had significantly improved grain yield and nitrogen-use efficiency (NUE) compared to the variety without that allele. Our results show that variation in NRT1.1B largely explains nitrate-use divergence between indica and japonica and that NRT1.1B-indica can potentially improve the NUE of japonica. © 2015 Nature America, Inc. All rights reserved.


Zou H.,Yangtze University | Zou H.,Beijing Academy of Agriculture and Forestry Sciences | Song Z.,Beijing Academy of Agriculture and Forestry Sciences | Wu Z.,Beijing Academy of Agriculture and Forestry Sciences | And 4 more authors.
Plant OMICS | Year: 2011

Pto has been reported to play important roles in plant disease responses. The cloning and characterization of the Pto-like gene named as ZmPto from maize was reported in this research. The open reading frame sequence of ZmPto was obtained by using RT-PCR. Sequence analysis showed that ZmPto encodes a polypeptide of 405 amino acids with predicted molecular mass of 45.0 kDa and pI of 6.01. Yeast two-hybrid analysis showed the interaction of ZmPto with ZmPti1. RT-PCR analysis indicated that the ZmPto expression is induced by salicylic acid (SA), abscisic acid (ABA), mannitol and salt suggesting that ZmPto may play important roles in both biotic and abiotic signal transduction pathway. Additionally, the ZmPto displays different expression patterns in different tissues indicating its multiple roles in plant.


Zou H.,Yangtze University | Zou H.,Chinese Academy of Agricultural Sciences | Li C.,Yangtze University | Liu H.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Australian Journal of Crop Science | Year: 2011

SnRK2s play a key role in the plant stress signaling transduction pathway. In previous study, ZmSPK1, a SnRK2 member has been cloned from maize. Expression pattern analyses showed that ZmSPK1 is induced by mannitol, salt and ABA. To investigate its role under stresses, in this study, ZmSPK1 was subcloned into the plant expression vector pGreen0029 under control of the cauliflower mosaic virus 35S promoter and transferred into Arabidopsis by Agrobacterium tumefaciens. When treated with salt, compared to wild type, transgenic Arabidopsis grow better, have higher seedling fresh weight (FW) and dry weight (DW), proline content and superoxide dismutase (SOD) activity; whereas, malondialdehyde (MDA) content and the relative electric conductivity of transgenic plants are kept to a relative lower level. Our results suggest that ZmSPK1 may play an important role in salt resistance.


Zou H.-W.,Yangtze University | Tian X.-H.,Yangtze University | Ma G.-H.,China National Hybrid Rice Research and Development Center | Li Z.-X.,Yangtze University
International Journal of Molecular Sciences | Year: 2013

Plant lipid transfer proteins (LTPs) are encoded by multigene families and play important roles in plant physiology. One full-length cDNA encoding an Arabidopsis LTP3 homologue was isolated from maize by RT-PCR and named as ZmLTP3. RT-PCR analysis indicated that the ZmLTP3 expression is induced by salicylic acid (SA), mannitol and salt. Furthermore, in different tissues the ZmLTP3 displayed different expression patterns, indicating that ZmLTP3 may play multiple roles in stress resistance. Over-expression of ZmLTP3 in wild-type Arabidopsis resulted in the increased salt tolerance. Under salt stress condition, compared to wild-type (WT) plants, transgenic Arabidopsis grew better, had higher seedling fresh (FW), dry weight (DW), seed yields, proline content and lower MDA content and relative electric conductivity level. Our results suggest that maize ZmLTP3 might encode a member of LTPs family and play roles in salt resistance. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


Tang L.,ShenYang Agricultural University | Ma D.R.,ShenYang Agricultural University | Xu Z.J.,ShenYang Agricultural University | Deng H.F.,China National Hybrid Rice Research and Development Center | And 2 more authors.
Plant Science | Year: 2011

Two representative weedy rice lines, three typical japonica varieties and three typical indica varieties were used for 6 pairs of reciprocal crosses. The morphological traits of twelve F1 hybrid lines, their parents and four elite cultivars were investigated for heterosis over mid-parent (HM), over parent (HP) and competitive heterosis (CH) analysis. Traits detected in weedy rice lines seemed larger than those in cultivars and excellent heterosis was produced in weedy rice crossing with japonica rice. Although weedy rice kept closer relationships with japonica rice compared to indica rice. But the heterosis of reciprocal crosses between weedy rice and japonica was closed to those of crosses between indica rice and japonica rice. In six of one hundred and eighteen weedy rice lines, the fertility restore gene for BT type cytoplasmic male sterility (BT-CMS) were detected. Weedy rice was very valuable germplasm resources with the abundant polymorphism. Meanwhile, the disadvantage, lodging, shattering and incompact plant type, should be modified by hybridization, backcross and multiple cross with japonica rice. Although it is difficult to use weedy rice directly, weedy rice may be available to breed both male sterile line and restorer line through improvement, developing japonica hybrid rice. © 2011 Elsevier Ireland Ltd.


PubMed | Chinese Academy of Sciences, Shanghai Academy of Agricultural science, Henan University of Science and Technology, CAS Institute of Genetics and Developmental Biology and 2 more.
Type: Journal Article | Journal: Nature genetics | Year: 2015

Asian cultivated rice (Oryza sativa L.) consists of two main subspecies, indica and japonica. Indica has higher nitrate-absorption activity than japonica, but the molecular mechanisms underlying that activity remain elusive. Here we show that variation in a nitrate-transporter gene, NRT1.1B (OsNPF6.5), may contribute to this divergence in nitrate use. Phylogenetic analysis revealed that NRT1.1B diverges between indica and japonica. NRT1.1B-indica variation was associated with enhanced nitrate uptake and root-to-shoot transport and upregulated expression of nitrate-responsive genes. The selection signature of NRT1.1B-indica suggests that nitrate-use divergence occurred during rice domestication. Notably, field tests with near-isogenic and transgenic lines confirmed that the japonica variety carrying the NRT1.1B-indica allele had significantly improved grain yield and nitrogen-use efficiency (NUE) compared to the variety without that allele. Our results show that variation in NRT1.1B largely explains nitrate-use divergence between indica and japonica and that NRT1.1B-indica can potentially improve the NUE of japonica.

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