China National Engineering Research Center for Floriculture

Beijing, China

China National Engineering Research Center for Floriculture

Beijing, China
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Liu X.,Beijing Forestry University | Liu X.,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding | Liu X.,China National Engineering Research Center for Floriculture | Wang Q.,Beijing Forestry University | And 8 more authors.
Molecular Biology Reports | Year: 2014

Oriental hybrid lily ‘Sorbonne’ was used to investigate molecular changes during the storage at 4 °C for dormancy-release besides physiology metabolic activity observations. In physiological mechanism, endogenous abscisic acid (ABA) concentration level of lily bulbs decreased as the cold preservation time increased, and it kept at a stable level after being preserved for 35 days. The level of soluble sugars concentrations also changed during the cold preservation time, and it increased as the cold preservation time raised to 49 days then decreased afterward. On molecule level, a new transcriptome providing comprehensive sequence profiling data of variation during dormancy-release in lily was constructed. 34,367 unigenes expressed differentially between the control and the treatment was analyzed. 14 genes including 8 MADS-box family genes, 4 genes related to plant hormone, and 2 DNA methylation genes were selected to identify the levels of their expression by qRT-PCR. Our results show that the decrease of ABA level during cold storage, as well as changes in plant hormone genes was correlated with dormancy-release; MADS-box family genes VRN2,FLC, FT, SOC1 a, as well as LFY, MIKC and ARF, MYB transcription factor were included in lily floral induction and DNA methylation was correlated to lily vernalization under low temperature. According to the results of the present studies, we predicted that plant hormone pathway, energy metabolic pathway, vernalization pathway, and DNA methylation played important roles during vernalization; these data provided the foundation for future studies of vernalization to induce flowering of lily. © 2014, Springer Science+Business Media Dordrecht.


Wang J.,Beijing Forestry University | Wang J.,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding | Wang J.,China National Engineering Research Center for Floriculture | Wang Q.,Beijing Forestry University | And 18 more authors.
Molecular Biology Reports | Year: 2014

Plants have continually confrontation with different abiotic stresses, including salt, low temperature, drought or hormone stress. The plants acclimate to the environmental stresses relating with the falls of the molecular mesh including the stress signal receiver, signal transcriptional regulation and the expression of functional and structure genes. Using the RNA-seq, we carried out a transcriptional analysis under cold treatment for investigating a profound comprehension of the signal network and molecular metabolisms reaction included in abiotic stress reaction for Lilium lancifolium. Our study identified 18,722 unigenes had demonstrated the resemblance to the known exact proteins in the Swiss-Prot protein database and classified them by Gene ontology into three primary kinds: cellular component, biological process, and molecular function, and then 15,898 unigenes aligned to existing sequences in the KEGG databases. Based on the transcriptome results of cold stress, more stress-related genes were identified and analyzed of their expressions in other abiotic stress treatments as 37 °C, ABA, JA and Na. Meanwhile, bioinformatics qRT-PCR analyses of stress genes as LlDREB1, LlAP2, LlNAC1, LlHOT, LlR2R3-MYB and LlCDPK revealed that novel candidate genes encoding ethylene responsive transporters and serine/threonine receptor-like kinases, which contributed to speculate the signal regulation pathway during the abiotic stresses; engineering genes could also boost the tolerance to stress, as protected and maintained the function and structure of cellular components. Our research conjectured the abiotic stress signal transduction pathway and identified the expected key ingredients regulating the stress tolerance in Lilium lancifolium, which would enable the in-depth molecular exploration of stress-tolerance mechanisms in lily. © 2014, Springer Science+Business Media Dordrecht.


Liu X.,Beijing Forestry University | Liu X.,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding | Liu X.,China National Engineering Research Center for Floriculture | Gu J.,Beijing Forestry University | And 8 more authors.
Molecular Biology Reports | Year: 2014

In our review, we highlighted the progresses made in molecular breeding of lily’s flowering, including the ABCDE models, the gene cloning, the establishment of regeneration system, the gene transformation methods, the transgene technology application in lily. Meanwhile, questions that were met at present in molecular breeding in flowering of lily were underlined, and we provide viable solutions. Although many researches on lily literature had been published in the world, in our review, we provided a valuable and unique resource and spring-board from which to understand or further study the molecular breeding in flowering of lily. © 2014, Springer Science+Business Media Dordrecht.


Cai M.,Beijing Forestry University | Tian M.,China Aerospace Science and Technology Corporation | Wang M.,Hangzhou Bureau of Garden and Cultural Relics | Pan H.-T.,Beijing Forestry University | And 3 more authors.
Acta Horticulturae | Year: 2012

In order to analysis of genetic diversity and relationship of Lagerstroemia indica cultivars, Amplified Fragment Length Polymorphisms (AFLP) and morphological markers were used to assess 37 L. indica cultivars, as well as L. subcostata and L. limii. Results showed that totally 278 bands were obtained from 5 AFLP primer pairs, 228 bands (82%) were polymorphic. Genetic similarity coefficient was varying from 0.64 to 0.93. According to 38 phenotype values of morphological characters, the genetic similarity coefficient was varying from 0.33 to 0.89. Cluster analysis by unweighted pair-group method with arithmetic mean (UPGMA) showed that both AFLP and morphological markers could well distinguish the L. indica cultivars and separated them into 3 distinct groups, which is generally in accordance with the known pedigree information. Moreover, the number of petal colours could divide cultivars with L. indica pedigree into two subgroups. Based on the two methods, the results were similar in the evaluations of genetic diversity and relationship among L. indica cultivars.


Huang J.,Beijing Forestry University | Huang J.,China National Engineering Research Center for Floriculture | Liu X.,Beijing Forestry University | Liu X.,China National Engineering Research Center for Floriculture | And 4 more authors.
Molecular Biology Reports | Year: 2014

The new transcriptomes provided comprehensive sequence profiling data of transcriptomic variation during vernalization in Lily Asiatic Hybrids 'Tiny ghost'. A number of 52,277,184 sequencing raw reads totaling 5.11 Gbp of the chilling treatment (4°C) sample and 39,466,176 sequencing raw reads totaling 3.85 Gbp of room temperature control (25°C) sample were assembled de novo into 68,718 unigenes with a mean length of 984 bp, and a total of 33,208 (45.6 %) unigenes were annotated by using public protein databases with a cut-off E value about 10-5. There are 6,153 unigenes of which were assigned to specific metabolic pathways by the Kyoto encyclopedia of genes and genomes. Gene Ontology analysis of the annotated unigenes revealed that the majority of sequenced genes were associated with signal transduction mechanisms, posttranslational modification, protein turnover and chaperones. In addition, the genes expression levels were compared just after vernalization completion between the cold treatment and room temperature control. There are 68,116 unigenes were differentially expressed, and hierarchical clustering analysis arranged 7,301 significantly differentially-expressed unigenes into 56 groups. Six genes related to the vernalization were selected to confirm their expression levels by using quantitative real-time polymerase chain reaction. Furthermore, typical vernalization unigenes VRN1 and VRN2 were identified, and also some vernalization-associated unigenes, such as CBF, SOC, TaAGL, AP2, LEA, LIM et al. were also annotated in the present study. As for VRN1 and VRN2, their expressions were consistent with some previous related studies. Also, this was the first time the vernalization genes VRN1 and VRN2 were founded in lily. According to the results of the present studies, we predicted that they would play an important role during vernalization in Lily Asiatic Hybrids; these data provided the foundation for the future studies of metabolism during vernalization of Asiatic lily. © 2014 Springer Science+Business Media.


Lu M.,Beijing Forestry University | Sun M.,Beijing Forestry University | Sun M.,China National Engineering Research Center for Floriculture | Zhang Q.-X.,Beijing Forestry University | Zhang Q.-X.,China National Engineering Research Center for Floriculture
Acta Horticulturae | Year: 2012

Bred and selected through iterations of inter-species hybridization, seedling selection and mutation selection, ground-cover chrysanthemum (Chrysanthemum × morifolium Ramat.) is more resistant than normal chrysanthemum under cold, dry, shady or polluted conditions. With the growing scarcity of water resources, it is becoming of great importance to breed and apply drought resistant varieties. Based on this requirement, we undertook research on drought resistance indices of ground-cover chrysanthemum, with the aim to determine the relative drought resistance of eight important varieties. Drought-stress investigations have been conducted on eight different specimens. Physiological indices (proline content, conductivity, chlorophyll content) and photosynthetic characteristics indices (photosynthetic rate, transpiration rate, stomatal conductance) were measured. Combined with morphological indices they were used to evaluate drought resistance of different varieties. Results suggested that drought resistance in the eight varieties investigated was found to be, from strong to weak: 'Pudijin', 'Beilinhong', 'Guoqingzi', 'Liujinsuiyue', 'Chongbanwanhuang', 'Luojinqian', 'Xinhuang' and 'Jindinghongxin'. It is also concluded that to combine morphological characteristics, photosynthetic and physiological indices is an effective method in evaluation of drought-resistance abilities. The study also sought to find out a method to evaluate overall drought resistance of chrysanthemum varieties cultivated in dry and alkaline soils of northern China.


Wu G.-S.,Beijing Forestry University | Wang J.,Beijing Forestry University | Wang J.,China National Engineering Research Center for Floriculture | Sun M.,Beijing Forestry University | And 5 more authors.
Acta Horticulturae | Year: 2012

Mei flower (Prunus mume Sieb. et Zucc.), as a representative of stanch national characteristics, has a long history of cultivation in China. Chinese Mei flower culture has a rich connotation. The development of the Mei flower industry has lead to Mei flower culture-themed tourism based on the exploration of Mei Flower culture and the design of its tourism products. In this study, the SWOT method was used to analyze the conditions required to develop Mei flower culture themed tourism in She County. Based on the analysis, the goals of themed tourism were clearly positioned. A series of the tourism products were designed and arranged with spatial and temporal distribution. Four itineraries, in the county town-Xu cun-Shang feng, county town-Fu ye, county town-Bei an zhen-Chang xi, and county town-Xiong cun, were designed to attract tourists all year round.


Song P.,Beijing Forestry University | Wang X.-F.,Beijing Forestry University | Cai M.,Beijing Forestry University | He D.,Beijing Forestry University | And 5 more authors.
Acta Horticulturae | Year: 2012

Flow cytometry refers to the technique where the measurement of the physical and chemical characteristics of particles including chromosomes, nuclei and cells is made as the particles pass in a fluid stream through a measuring point surrounded by an array of detectors. Compared with chromosome counting using microscopy methods, it is a reliable method that is accurate, rapid and reproducible. To prepare nucleus suspensions, leaves were pretreated, and five different isolation buffers (Arumuganathan's buffer, Otto buffers, Tris-MgCl2 buffer, Galbraith's buffer and Lysis buffer (Jin, 2007)) were used to prepare the nucleus suspensions. To reduce the impact that phenolic compounds had on the samples, PVP was added to the isolation buffer. The comparisons showed that pretreatment of the leaves enhanced the isolation efficiency, the sample prepared by Lysis buffer showed the best resolution in Lagerstroemia indica, with the smallest variation coefficient (CV) 4.73. The sample prepared by PVP-added-Otto buffers showed the best resolution in Lagerstroemia subcostata, with the smallest variation coefficient (CV) 4.66. Flow cytometry is an ideal method for the identification of polyploids in Lagerstroemia indica and Lagerstroemia subcostata, as the method readily distinguishes between chimeras and pure tetraploids.


Cai M.,Beijing Forestry University | Meng R.,Beijing Forestry University | Pan H.-T.,Beijing Forestry University | Pan H.-T.,China National Engineering Research Center for Floriculture | And 8 more authors.
Conservation Genetics Resources | Year: 2010

We developed 17 microsatellite loci from Lagerstroemia caudata to analyze the genetic diversity and population structure of this endangered species. These loci provided markers with polymorphisms of 2-11 alleles per locus within 48 individuals from Hubei, China. The observed and expected heterozygosities ranged from 0.06 to 0.77 and 0.31 to 0.83, respectively. Most cross-species amplification was successful among five Lagerstroemia species and one Lawsonia species. These SSR loci were expected to be useful tools for population studies both in L. caudata and related species and genera. © Springer Science+Business Media B.V. 2010.


He D.,Beijing Forestry University | He D.,Henan Agricultural University | Liu Y.,Beijing Forestry University | Cai M.,Beijing Forestry University | And 5 more authors.
Plant Breeding | Year: 2014

Lagerstroemia (crape myrtle) are famous ornamental plants with large pyramidal racemes, long flower duration and diverse colours. Genetic maps provide an important genomic resource of basic and applied significance. A genetic linkage map was developed by genotyping 192 F1 progeny from a cross between L. caudata (female) and L. indica ('Xiang Xue Yun') (male) with a combination of amplification fragment length polymorphisms (AFLP) and simple sequence repeats (SSR) markers in a double pseudo-testcross mapping strategy. A total of 330 polymorphic loci consisting of 284 AFLPs and 46 SSRs showing Mendelian segregation were generated from 383 AFLP primer combinations and 150 SSR primers. The data were analysed using JoinMap 4.0 (evaluation version) to construct the linkage map. The map consisted of 20 linkage groups of 173 loci (160 AFLPs and 13 SSRs) covering 1162.1 cM with a mean distance of 10.69 cM between adjacent markers. The 20 linkage groups contained 2-49 loci and ranged in length from 7.38 to 163.57 cM. This map will serve as a framework for mapping QTLs and provide reference information for future molecular breeding work. © 2013 Blackwell Verlag GmbH.

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