Liu Y.,Sichuan University |
Liu Y.,China National And Local Joint Engineering Labo Of Energy Plants Bio Fuel Oil Prep And Util |
Zhang X.,Sichuan University |
Zhang X.,China National And Local Joint Engineering Labo Of Energy Plants Bio Fuel Oil Prep And Util |
And 8 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2014
This study aimed to optimize the degradation rate of phorbol ester of Jaropha curcas seed cake through solid-state fermentation of Klebsiella variicola. Firstly, by the single factor experiments, the effects of fermentation temperature, inoculum amount, initial amount of water and fermentation time were determined. Then Design Expert8.0 was used to analyze and optimize the model of phorbol esters degradation rate by three factors (fermentation temperature, inoculum amount and initial amount of water) through response surface methodology. A model was then set up, its validity verified, and the degradation rate of curcin measured. The model was significant (P < 0.001), fitting well. In confirming experiments, under the condition of fermentation temperature 37 °C, inoculation (V/m) 33%, the initial water content 105% (V/m), after fermentation for 48 hours, the phorbol ester degradation rate was 84.30% and curcin degradation rate 63.21%. The results suggested that with optimization of the response surface methodology, K. variicola can degrade phorbol ester efficiently and at the same time degrade curcin.