Tao L.,State Key Laboratory of Ceramic |
Xiang H.,State Key Laboratory of Ceramic |
Xu H.,State Key Laboratory of Ceramic |
Sun J.,State Key Laboratory of Ceramic |
And 3 more authors.
Accreditation and Quality Assurance
The proficiency-testing scheme (CNCA-07-06) concerning lead and cadmium determination extracted from glazed ceramic surfaces involved 43 Chinese laboratories as participants, and has been well implemented. All the test materials passed the statistical homogeneity tests, and were deemed suitable for distribution. Reference values were determined from the obtained results and the performance of participants was based on z-scores. As a result, the majority of participants obtained satisfactory z-scores and laboratories that need to revise their procedures were singled out. Overall, about 8.5% of all results submitted in this PT round were unsatisfactory in terms of z-scores (22 out of 258 total data sets submitted), while the percentage of doubtful z-scores was 4.6% (12 out of 258 total data sets). Besides, the factors that might be associated with unsatisfactory results were also evaluated and suggestions were made to help improve the performance of laboratories in this PT. © Springer-Verlag 2009. Source
Wang L.-B.,Shanghai Institute for Food and Drug Control |
Yang M.-C.,Shanghai Institute for Food and Drug Control |
Chen Z.-K.,Shanghai Institute for Food and Drug Control |
Lu M.,Shanghai Institute for Food and Drug Control |
And 3 more authors.
This proficiency testing program is established to evaluate the pharmaceutical preparation analysis capacity of laboratories recommended by 18 countries and economies. It was authorized by Asia Pacific Laboratory Accreditation Cooperation (APLAC), and organized by Shanghai Institute for Food and Drug Control (SIFDC) and China National Accreditation Service for Conformity Assessment (CNAS). The 0.3σ test is used to evaluate the homogeneity and stability of the proficiency testing sample. The results of the laboratories were assessed by Z-score. The robust average and the robust standard deviation of the participants' results were calculated as assigned value and standard deviation for performance assessment of hydrochlorothiazide and captopril using robust statistics. Thirty-three of 38 laboratories recommended by 18 countries and economies sent their results back. Twenty-four laboratories' results were observed as satisfactory. Five laboratories were identified as having reported at least one questionable result. Four laboratories were identified as having reported at least one unsatisfactory result. Source
Chen S.,Xiamen Entry Exit Inspection and Quarantine Technology Center |
Zhang Y.,Xinglin Entry Exit Inspection and Quarantine Bureau |
Li H.,China National Accreditation Service for Conformity Assessment |
Wang J.,Xiamen Entry Exit Inspection and Quarantine Technology Center |
And 3 more authors.
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and lab-on-a-chip system were used to identify 62 commercial fish species in Taiwan Strait. The fish species include 10 groupers, 12 bream species, 9 Sciaenidae species, 5 puffer species and 26 other fish species. A fragment of 464bp length of mitochondrial cytoehrome b gene was amplified by PCR and the products were digested with restriction enzymes DdeI, HaeIII and NlaIII, individually. The fragments generated after digestion were further resolved on the DNA chip. The results demonstrated that PCR-RFLP analysis and lab-on-a-chip system provided a fast, easy, automated and reliable analysis approach and it will be useful for the control of the adulteration of food with fish tissue content in Taiwan Strait. © 2014 Elsevier Ltd. Source
Zhang J.,Beijing Entry Exit Inspection and Quarantine Bureau |
Xu M.,Linyi Entry Exit Inspection and Quarantine Bureau |
Wang X.,Chinese Academy of Sciences |
Wang Y.,China National Accreditation Service for Conformity Assessment |
And 6 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
To develop a specific, rapid and convenient method based on molecular motor biosensor to detect food-borne rotavirus. A specific probe was encompassed the conservative region of rotavirus's VP7 segment, and a molecular motor detect device was constructed by connecting probes to F0F1-ATPase molecular motor through biotin-streptavidin system. This biosensor's sensitivity was 0.005 ng/mL for rotavirus RNA. Extracted virus RNA was conjugated with the biosensor separately, at the same time ATP was synthesized. By comparing fluorescence intensity, we can detect rotavirus RNA in samples. This method possessed specificity for rotavirus, without any cross-reaction with Hepatitis A virus and noroviris, and it could be accomplished within 1 h. We detected 15 samples using this method and the results were compared with RT-PCR results. This method is sensitive and specific for rotavirus, and it can be used to detect food-borne rotavirus. © 2013 by the Institute of Microbiology, the Chinese Academy of Sciences and the Chinese Society for Microbiology. Source
Wang Z.,Sichuan University |
Zhang J.,Sichuan University |
Tang D.-Z.,China National Accreditation Service for Conformity Assessment |
Wang Y.-B.,China National Accreditation Service for Conformity Assessment |
Hou Y.-P.,Sichuan University
Journal of Forensic Medicine
MicroRNA (miRNA) belongs to a class of small, non-coding RNA molecules that contains 18-25 nucleotides and regulates gene expression at post-transcriptional level. Many miRNAs are highly conserved and display timing- and tissue-specific expression. With the advance of the miRNA detection technologies, miRNA has been introduced to forensic science as a potentially novel set of genetic markers of forensic body fluid identification, species identification and PMI estimation. In this article, the detection methodologies of miRNA are reviewed, and their potential applications in forensic practice and research future are also discussed. Source