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Xiao X.,China Military Institute of Chinese Meteria Medica | Wang J.,China Military Institute of Chinese Meteria Medica | Zhao Y.,China Military Institute of Chinese Meteria Medica | Wang Y.,Chinese Academy of Sciences | Xiao P.,Chinese Academy of Sciences
Zhongguo Zhongyao Zazhi | Year: 2010

Medicinal nature theory of Chinese medicine is the difficult and hot issue in the basic research of Chinese medicine (CM), but has not yet obtained some important breakthrough until now. The Cold and Heat syndromes is considered as the capital differentiation of CM in clinic; Cold and Hot is the primary medicinal nature of CM. Treating the Cold with Heat, the Heat with Cold'is the main therapeutic principle of CM. But, whether the Cold and Hot of medicinal nature objectively exists? Whether/how to establish a set of objectives and feasible appraisal methodologies? How to apply the theoratical and research findings of Medicinal nature in clinical practice? In recent years, a new road for ourselves to carry out a series of explorations and researches on the Cold and Hot nature of CM from the thermodynamic view has been opened, and the doctrine of "Thermodynamic outlook of Chinese medicinal nature" has been proposed firstly by our research group. Consequently, we have established the research model, "coming from clinic, verifying in experiment and returning to the clinic", on the medicinal nature of CM, and developed a set of appraisal methodologies of the Cold and Hot nature of Chinese medicine based on biothermokinetics, such as the Cold/Hot plate differentiating system, microcalorimetry, evidence-based medical analysis for medicinal nature of CM. Based on these methods, a systematical investigation has been done focusing the energy transfer and thermal change in the metabolism progress of organism and the intervention effects of different Chinese medicines on this progress from the experiment to the clinic, in vitro and in vivo. Our studies have essentially elucidated the objectivity of the differences between the Cold and Hot nature of Chinese medicine as well as the scientific connotation of "treating the Cold with Heat, the Heat with Cold", provided a novel and perspective approach for investigating the medicinal nature theory of Chinese medicine, further supplied some new technological supports for the modernization of CM.

Zang Q.-C.,China Military Institute of Chinese Meteria Medica | Zang Q.-C.,Jiangxi University of Traditional Chinese Medicine | Wang J.-B.,China Military Institute of Chinese Meteria Medica | Kong W.-J.,China Military Institute of Chinese Meteria Medica | And 9 more authors.
Journal of Separation Science | Year: 2011

The fingerprints of artificial Calculus bovis extracts from different solvents were established by ultra-performance liquid chromatography (UPLC) and the anti-bacterial activities of artificial C. bovis extracts on Staphylococcus aureus (S. aureus) growth were studied by microcalorimetry. The UPLC fingerprints were evaluated using hierarchical clustering analysis. Some quantitative parameters obtained from the thermogenic curves of S. aureus growth affected by artificial C. bovis extracts were analyzed using principal component analysis. The spectrum-effect relationships between UPLC fingerprints and anti-bacterial activities were investigated using multi-linear regression analysis. The results showed that peak 1 (taurocholate sodium), peak 3 (unknown compound), peak 4 (cholic acid), and peak 6 (chenodeoxycholic acid) are more significant than the other peaks with the standard parameter estimate 0.453, -0.166, 0.749, 0.025, respectively. So, compounds cholic acid, taurocholate sodium, and chenodeoxycholic acid might be the major anti-bacterial components in artificial C. bovis. Altogether, this work provides a general model of the combination of UPLC chromatography and anti-bacterial effect to study the spectrum-effect relationships of artificial C. bovis extracts, which can be used to discover the main anti-bacterial components in artificial C. bovis or other Chinese herbal medicines with anti-bacterial effects. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Ye T.,China Military Institute of Chinese Meteria Medica | Ye T.,Nanchang University | Jin C.,China Military Institute of Chinese Meteria Medica | Zhou J.,China Military Institute of Chinese Meteria Medica | And 8 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2011

Musk is a precious and wide applied material in traditional Chinese medicine, also, an important material for the perfume industry all over the world. To establish a rapid, cost-effective and relatively objective assessment for the quality of musk, different musk samples, including authentic, fake and adulterate, were collected. A oxide sensor based electronic nose (E-nose) was employed to measure the musk samples, the E-nose generated data were analyzed by principal component analysis (PCA), the responses of 18 sensors of E-nose were evaluated by loading analysis. Results showed that a rapid evaluation of complex response of the samples could be obtained, in combination with PCA and the perception level of the E-nose was given better results in the recognition values of the musk aroma. The authentic, fake and adulterate musk could be distinguished by E-nose coupled with PCA, sensor 2, 3, 5, 12, 15 and 17 were found to be able to better discriminate between musk samples, confirming the potential application of an electronic instrument coupled with chemometrics for a rapid and on-line quality control for the traditional medicines. © 2011 Elsevier B.V.

Qiu L.-L.,China Military Institute of Chinese Meteria Medica | Qiu L.-L.,Jiangxi University of Traditional Chinese Medicine | Chen L.-H.,China Military Institute of Chinese Meteria Medica | Chen L.-H.,Chengdu University of Traditional Chinese Medicine | And 6 more authors.
Yaoxue Xuebao | Year: 2012

This study aimed to establish a novel method to screen out the combined components of multifractions traditional Chinese medicine (TCM), so that the internal relationship between multi-ingredients could be objectively assessed and the proportioning ratio could be optimized. Taking antiviral effect on neuraminidase activity of influenza virus as the evaluating indicator and using Box-Behnken response surface methodology, the main effective ingredients of Shuanghuanglian injection (SHL) were screened. Meanwhile, the relationship between active ingredients was discussed. Taking SHL as a comparison, the optimum proportioning ratio was predicted. The results indicated that chlorogenic acid, cryptochlorogenic acid, caffeic acid and baicalin have comparatively strong antiviral activity against influenza virus. Moreover, antagonistic action existed between chlorogenic acid and cryptochlorogenic acid, whereas synergistic action between caffeic acid and other components. The optimum proportioning ratio resulted from fitted model is: chlorogenic acid, cryptochlorogenic acid, caffeic acid and baicalin (107 μg·mL -1: 279 μg·mL-1 : 7.99 μg·mL -1 : 92 μg·mL-1). The antiviral activity of the recombined components is stronger than that of SHL, which was consistent with the experiment results (P < 0.05). Box-Behnken response surface methodology has the advantages of general-screening, high-performance and accurate-prediction etc, which is appropriate for screening the combined components of multi-fractions TCM and the optimization of the proportioning ratio. The proposed method can serve as a technological support for the development of modern multi-fractions TCM.

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