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Li G.T.,China Meitan General Hospital | Li X.F.,Peking University | Li J.,Beijing Fengtai Hospital | Liu Y.J.,China Meitan General Hospital | Xu H.M.,Beijing Fengtai Hospital
Gynecologic and Obstetric Investigation | Year: 2015

Background/Aims: Postpartum hemorrhage (PPH) is a life-threatening condition with a worldwide occurrence. The purpose of this study is to evaluate the efficacy and safety of a reflexed compression suture in controlling severe atonic PPH with placenta accreta. Methods: Eleven women with severe PPH due to uterine inertia or placenta accreta were administered the reflexed compression suture. The procedure was to reflex the fundus onto the anterior wall of the uterus for compressing hemostasis and to form a 'belt-like' binding suture to reinforce the effectiveness of pressing the myometrium. Results: Ten of the 11 women (90.9%) were successfully treated with the suture, and the uterus was preserved. None of these patients developed complications related to this method. Only in 1 patient with placenta increta could the bleeding not be stanched, and a peripartum hysterectomy was performed. Two women had pregnancies after the suture. Conclusion: The reflexed compression suture is a simple, swift, safe and effective technique of controlling uterine atonic bleeding, particularly in patients with an abnormally adherent placenta. The advantage of not having to conduct a hysterotomy also lies in reducing the duration of anesthesia and blood loss. © 2015 S. Karger AG, Basel.

Li G.T.,China Meitan General Hospital | Li X.F.,Peking University | Liu Y.J.,China Meitan General Hospital | Li W.,China Meitan General Hospital | Xu H.M.,Beijing Fengtai Hospital
Archives of Gynecology and Obstetrics | Year: 2015

Objective: To evaluate the efficacy and safety of a symbol ‘‘&’’ compression suture technique in controlling severe atonic postpartum hemorrhage with placenta previa accreta during cesarean delivery. Methods: Nine women with heavy postpartum bleeding from uterine inertia and placenta previa percret, which did not react to conventional initial management protocols, were underwent the suture in the shape of symbol ‘‘&’’ in China Meitan General Hospital. The suture procedure was to staple the anterior and posterior walls (of the lower uterine segment as well as corpus uterus) together using number 1 chromic catgut, with a cross at about 2 cm above the upper boundary of lower uterine segment. Results: Symbol ‘‘&’’ compression suture was capable of stanching the postpartum hemorrhage immediately in all nine women. None of these patients developed complications related to this method. Subsequent pregnancies after the suture were occurred in two women and delivered with repeat cesarean section. Conclusion: Symbol ‘‘&’’ compression suture is a simple, safe and highly effective technique to control the treatment- resistant uterine atonic bleeding, particularly in previous cesarean scar at lower segment and placenta previa accreta. © Springer-Verlag Berlin Heidelberg 2014.

Liu J.,Peking University | Zhao L.,Peking University | Xue Y.,Peking University | Shi J.,Peking University | And 13 more authors.
Biological Psychiatry | Year: 2014

Background: Conditioned fear memories can be updated by extinction during reconsolidation, and this effect is specific to the reactivated conditioned stimulus (CS). However, a traumatic event can be associated with several cues, and each cue can potentially trigger recollection of the event. We introduced a technique to target all diverse cues associated with an aversive event that causes fear.Methods: In human experiments, 161 subjects underwent modified fear conditioning, in which they were exposed to an unconditioned stimulus (US) or unreinforced CS to reactivate the memory and then underwent extinction, spontaneous recovery, and reinstatement. In animal experiments, 343 rats underwent contextual fear conditioning under a similar protocol as that used in the human experiments. We also explored the molecular alterations after US reactivation in rats.Results: Presentation of a lower intensity US before extinction disrupted the associations between the different CS and reactivated US in both humans and rats. This effect persisted for at least 6 months in humans and was selective to the reactivated US. This procedure was also effective for remote memories in both humans and rats. Compared with the CS, the US induced stronger endocytosis of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid glutamate receptors 1 and 2 and stronger activation of protein kinase A, p70S6 kinase, and cyclic adenosine monophosphate response element binding protein in the dorsal hippocampus in rats.Conclusions These findings demonstrate that a modified US retrieval extinction strategy may have a potential impact on therapeutic approaches to prevent the return of fear. © 2014 Society of Biological Psychiatry.

Song L.,Peking Union Medical College | Su M.,Peking Union Medical College | Wang S.,Peking Union Medical College | Zou Y.,Peking Union Medical College | And 6 more authors.
Journal of Cellular and Molecular Medicine | Year: 2014

The molecular mechanisms that drive the development of cardiac hypertrophy in hypertrophic cardiomyopathy (HCM) remain elusive. Accumulated evidence suggests that microRNAs are essential regulators of cardiac remodelling. We have been suggested that microRNAs could play a role in the process of HCM. To uncover which microRNAs were changed in their expression, microRNA microarrays were performed on heart tissue from HCM patients (n = 7) and from healthy donors (n = 5). Among the 13 microRNAs that were differentially expressed in HCM, miR-451 was the most down-regulated. Ectopic overexpression of miR-451 in neonatal rat cardiomyocytes (NRCM) decreased the cell size, whereas knockdown of endogenous miR-451 increased the cell surface area. Luciferase reporter assay analyses demonstrated that tuberous sclerosis complex 1 (TSC1) was a direct target of miR-451. Overexpression of miR-451 in both HeLa cells and NRCM suppressed the expression of TSC1. Furthermore, TSC1 was significantly up-regulated in HCM myocardia, which correlated with the decreased levels of miR-451. As TSC1 is a known positive regulator of autophagy, we examined the role of miR-451 in the regulation of autophagy. Overexpression of miR-451 in vitro inhibited the formation of the autophagosome. Conversely, miR-451 knockdown accelerated autophagosome formation. Consistently, an increased number of autophagosomes was observed in HCM myocardia, accompanied by up-regulated autophagy markers, and the lipidated form of LC3 and Beclin-1. Taken together, our findings indicate that miR-451 regulates cardiac hypertrophy and cardiac autophagy by targeting TSC1. The down-regulation of miR-451 may contribute to the development of HCM and may be a potential therapeutic target for this disease. © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Zhang M.,China Meitan General Hospital
Chinese Journal of Cardiology | Year: 2016

Objective: This study is designed to observe the effect of ischemic postconditioning in rats underwent acute myocardial ischemia/reperfusion injury and to investigate the related mechanism. Methods: A total of 30 SD rats were randomly divided into sham operation group ( control group, n = 10) , ischemia/reperfusion group ( IR group, n = 10) and ischemic postconditioning group (PC group, n= 10) based on random number table. Rats in IR group underwent 30 minutes myocardial ischemia by occlusion of the proximal portion of left anterior descending (LAD) coronary artery followed by 2 hours reperfusion. In control group, there was no IR intervention. In PC group, at the start of reperfusion, three cycles of 30 seconds reperfusion and 30 seconds LAD reocclusion preceded the 2 hours of reperfusion. The hemodynamic values were measured via a cannula inserted into the right common carotid artery. The area at risk was assessed by Evans blue staining and the infarct size as measured by TTC staining. Western blot and Real time PCR were respectively used to assess the expression of predicted target gene Bim and microRNA-2l4( miR-214) in the area at risk at the end of 2 hours reperfusion. Results: ( 1 ) The hemodynamic monitoring in different groups: the left ventricular systolic pressure ( LVSP) , ± dp/dtmax, and heart rate of IR group and PC group were lower than those of control group, but left ventricular end-diastolic pressure ( LVEDP) was higher than that of control group (all P < 0.05 ). The LVSP and ± dp/dtmax of PC group were higher than those of IR group, and LVEDP was lower than that of IR group ( all P < 0.05 ). (2) Myocardial ischemia area and infarction range in different groups; there was no statistically difference in the proportion of area at risk ( AAR) in left ventricle ( LV) ( AAR/LV) between PC group and IP group ( (27.00 + 7.55) % vs. (26.67 + 11.68 ) % , P > 0.05 ). The proportion of infarct size in the area at risk( IS/AAR) of PC group was lower than that of IR group ( ( 30.67 ±3.5l)% vs. (48.67 ±4.62)%, P < 0.05 ).(3) The expression of Bim protein in rats ischemic myocardial tissue in different groups; the expression of Bim protein in ischemic myocardial tissue of IR group was higher than that of control group (2.34 ±0.15 vs. 0.75 ± 0.05 , P < 0.05 ) , and that of PC group was lower than IR group ( 1.25 ± 0.14 vs. 2.34 ± 0.15 , P < 0.05). ( 4 ) The expression of of miR-214 in rats ischemic myocardial tissue in different groups; the expression of miR-214 of IR group was lower than that of control group(0.20 ±0.04 vs. 1.00,P < 0.01) , and that of PC group was higer than that of IR group ( 0.85 ± 0.20 vs. 0.20 ± 0.04, P < 0.01). There was no statistically difference between PC group and control group ( P > 0.05 ). Conclusions: Postconditioning could significantly decrease the ischemia/reperfusion injury by reducing the infarct size and improve cardiac function in this in vivo rat model. The expression of Bim in postconditioning group is significantly depressed, which may play an important role in the protection process of postconditioning, and the downregulation of Bim might be mediated with the increase of miR-214 expression. Copyright © 2016 by the Chinese Medical Association.

Liu L.,China Meitan General Hospital
Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery | Year: 2011

To investigate the application and mechanism of tissue-engineered skin with mouse embryonic fibroblasts (MEFs) for the full-thickness skin defects on mice. The MEFs and fibroblasts were cultured and seeded in scaffold made of rat tail collage. ELISA method was used for detection of secretory function. The full-thickness skin defects were created on mice and covered by MEFs-scaffold complex (experimental group), or FBs-scaffold complex (control group 1), or scaffold only (control group 2). The process of wound healing was evaluated by observation of the re-epithelization rate. Microvessel density (MVD) and vimentin within the wound sites were also detected with immunohistochemistry staining technique to describe the characteristics of wound healing. Hoechst 33342 staining was performed to trace MEFs'fate. MEFs scaffold group had higher level secretion of IL-6 and lower of TGF-beta1 than FBs scaffold group (P<0.05). Compared with wounds in control groups, the wounds in MEFs group healed markedly fast (P<0.05) and the MVD was significantly higher (P <0.05). The fibroblasts in the wounds of MEFs group were arranged regularly and the MEFs decreased during the healing process. The MEFs-scaffold complex can promote wound healing with less scar formation. MEFs may have an inducing effect on the wound healing.

Zhangtao Z.,China Meitan General Hospital
Safety Science | Year: 2010

Introduction: To study the epidemiological characteristics on fatal accidents of China. Method: Adopt descriptive research approach to describe accident report data during January 1st, 2001 to December 31st, 2008 that caused more than nine deaths each, which were published by the State Administration of Work Safety subordinated by State Council. Result: Nine hundred and sixty two occupational-related fatal accidents (caused more than nine deaths) are found in 31 provinces, causing 17,112 deaths, 8222 injuries. Discussion: Safety accidents frequently happened these years in China. Safety accidents frequently happened these years in China. The occurrence of safety accidents can be associated with risk factors from multiple perspectives such as workers, occupational environment, social environment, natural environment, regulations, and injury objects. Strength of supervision and adjustment of public health policy are needed in China to decrease the occurrence rate of fatal accidents. © 2010 Elsevier Ltd.

Zhou Y.,Capital Medical University | Li Y.,Beijing Military General Hospital | Mu T.,China Meitan General Hospital
Biological and Pharmaceutical Bulletin | Year: 2015

High-mobility group box 1 (HMGB1) and its natural receptor, Toll-like receptor-4 (TLR4), are involved in various infectious or noninfectious diseases including hemorrhagic shock. HMGB1 neutralizing antibody (anti-HMGB1 monoclonal antibody (mAb)) treatment was shown to alleviate ischemia-reperfusion injury effectively. The aim of this study was to explore whether and by what mechanisms anti-HMGB1 mAb attenuates hemorrhagic shock and resuscitation (HS/R)-induced cardiac injury. Employing rat HS/R models, we found that anti-HMGB1 mAb treatment improved HS/R-induced cardiac function deterioration, attenuated cardiac enzyme elevation, improved ATP loss, and protected cardiac tissue. Anti-HMGB1 mAb also inhibited the production of inflammatory factors interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α). Moreover, anti-HMGB1 mAb reduced apoptotic responses by suppressing activated caspase-3 and reversing apoptotic gene expression of capase-3, Bax, and Bcl-2 in rat cardiac tissue. Moreover, anti-HMGB1 mAb decreased HS/R-induced HMGB1 and TLR4 expression elevation. We further confirmed that anti-HMGB1 mAb inhibited lipopolysaccharide-activated HGMB1 and TLR4 expression and decreased inflammatory factors IL-1β, IL-6, and TNF-α at the cellular level. It was concluded that anti-HMGB1 mAb treatment protects rats from cardiac injury induced by HS/R, and the beneficial effects may be related to its inhibitory effects on the HMGB1-TLR4 axis. © 2015 The Pharmaceutical Society of Japan.

Liu Y.L.,China Meitan General Hospital
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2012

To observe the impacts of electroacupuncture (EA) at Sanyinjiao (SP 6) on labor and assess its safety. The random and single-blind method was adopted to divide 111 cases of the puerperas of natural delivery into an EA group (38 cases), a sham-acupuncture group (37 cases) and a blank control group (36 cases). In EA group, Sanyinjiao (SP 6) was selected and stimulated with Han's acupoint nerve stimulator (HANS). In the sham-acupuncture group, the puerperas were stimulated only with the needle tube, needle removed in advance and they felt subjectively to be needled. Afterward, the filiform needle was attached to Sanyinjiao (SP 6) with the needle tip wrapped with the adhesive plaster in advance, then HANS which one conducting wire was cut off in was connected. In the blank control group, no any treatment was applied, but the same indices were recorded at corresponding time points. The blood pressure and the heart rate of the puerperas as well as the heart rate of fetus were measured in 20 min of EA and 30 min after needle removal in three groups separately. The bleeding 24 h after labor and 1 min Apger score of the newborns were observed. In EA group, the active phase of the 1st labor stage ((4.38 +/- 1.76)h) was shorter than that ((5.28 +/- 2.41)h) in the blank control group and that ((5.38 +/- 2.36)h) in the sham-acupuncture group, presenting statistically significant differences (all P < 0.05). In the latent phase of the 1st labor stage and the 2nd and 3rd stages, in the comparison of the blood pressure and the heart rate of the puerperas as well as the heart rate of fetus after EA, the bleeding 24 h after labor and 1 min Apger score of the newborns among three groups, there were no statistically significant differences (all P > 0.05). EA at Sanyinjiao (SP 6) can shorten the duration of the active phase of the 1st labor stage. It is safe for either the puerpera or the fetus and can assist the parturition quality in clinic.

Sun Y.,China MeiTan General Hospital | Zhang T.,Chinese Academy of Sciences | Li L.,China MeiTan General Hospital | Wang J.,China MeiTan General Hospital
Kidney and Blood Pressure Research | Year: 2015

Background/Aims: Endoplasmic reticulum (ER) stress is one of the intrinsic apoptosis pathways, and cardiac apoptosis can occur in cardiovascular diseases, such as hypertension. However, the mechanisms by which ER stress leads to apoptosis remain enigmatic, particularly in the progression from cardiac hypertrophy to diastolic heart failure due to hypertension. Methods: We used spontaneously hypertensive rats (SHRs) to investigate possible signalling pathways for ER stress. Results: We found that cardiac protein and mRNA levels of glucose-regulated protein 78 were up-regulated. In addition, the CHOP-And caspase-12-dependent pathways, but not that of JNK, were activated in the SHR rats. Conclusions: These results suggest that ER stress can contribute to myocardial apoptosis during hypertensive disease. © 2015 S. Karger AG, Basel.

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