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Klapoetke S.,KBI Biopharma | Xie M.H.,China Medicine Corporation
Journal of Pharmaceutical and Biomedical Analysis | Year: 2017

Protein-level partial reduction was investigated as a novel sample preparation technique to characterize proteins with cystine knots or complex disulfide linkages. Human Factor Xa containing twelve disulfide bonds was selected as a model protein to demonstrate this methodology. Five in twelve disulfide linkages were characterized through conventional non-reduced samples while the other seven disulfide linkages containing cystine knots were successfully characterized though partially reduced samples. Each disulfide linkage was confirmed through product ions generated by an UPLC-ESI QTOF MS system equipped with data independent collision-induced dissociation (CID) acquisition. Free cysteines in the sample were also determined in this study. © 2016 Elsevier B.V.


Wu J.,Peking Union Medical College | Wu J.,China Medicine Corporation | Zhao R.,China Medicine Corporation | Chen B.,China Medicine Corporation | Yang M.,Peking Union Medical College
Food Chemistry | Year: 2011

Zearalenone (ZEA) is one of the most widely distributed mycotoxins produced by Fusarium species, responsible for important diseases of barley. The present work shows the results of a rapid and accurate method to quantify ZEA in barley. The method uses the Quick Easy Cheap Effective Rugged and Safe method (QuEChERS) for purification and high-performance liquid chromatography coupled with an evaporative laser scattering detector (HPLC-ELSD) for detection and quantification of the toxin. Barley samples were extracted with methanol and used acetonitrile-water-methanol (46:46:8, v/v) as mobile phase. Average recoveries of ZEA from barley spiked at levels of 0.1-10 μg/g ranged from 83.0% to 91.5%, with relative standard deviations of <6%. The detection limit was 1.56 ng/g based on a signal-to-noise ratio of 3:1. Using this method, we have analysed a total of 175 samples of 35 functional food marketed in China. The results showed that the overall level of ZEA contamination was relatively low (5.14%) and the presence of ZEA was found in nine samples at concentrations ranging from 11.09 to 26.54 ng/g. © 2010 Elsevier Ltd. All rights reserved.


Trademark
China Medicine Corporation | Date: 2012-03-06

Dietary and nutritional supplements; Dietary and nutritional supplements used for weight loss; Dietary supplement for eliminating toxins from the intestinal tract; Food supplements; Herbal supplements; Herbal teas for medicinal purposes; Medicinal drinks; Medicinal herb extracts; Medicinal herbal extracts for medical purposes; Medicinal herbs; Medicinal tea; Natural herbal supplements.


Trademark
China Medicine Corporation | Date: 2012-03-06

Dietary and nutritional supplements; Dietary and nutritional supplements used for weight loss; Dietary supplement for eliminating toxins from the intestinal tract; Food supplements; Herb teas for medicinal purposes; Herbal supplements; Herbal teas for medicinal purposes; Medicinal drinks; Medicinal herb extracts; Medicinal herbal extracts for medical purposes; Medicinal tea; Natural herbal supplements.


Trademark
China Medicine Corporation | Date: 2012-03-06

Dietary and nutritional supplements; Dietary and nutritional supplements used for weight loss; Dietary supplement for eliminating toxins from the intestinal tract; Food supplements; Herb teas for medicinal purposes; Herbal supplements; Herbal teas for medicinal purposes; Medicinal drinks; Medicinal herb extracts; Medicinal herbal extracts for medical purposes; Medicinal tea; Natural herbal supplements.


Trademark
China Medicine Corporation | Date: 2012-03-06

Dietary and nutritional supplements; Dietary and nutritional supplements used for weight loss; Food supplements.


Trademark
China Medicine Corporation | Date: 2011-12-27

Herb teas for medicinal purposes; Herbal supplements; Herbal teas for medicinal purposes; Natural herbal supplements.


Trademark
China Medicine Corporation | Date: 2012-01-10

Herb teas for medicinal purposes; Herbal supplements; Herbal teas for medicinal purposes; Natural herbal supplements.


Trademark
China Medicine Corporation | Date: 2012-01-10

Herb teas for medicinal purposes; Herbal supplements; Herbal teas for medicinal purposes; Natural herbal supplements.


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