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Liu Y.,Tangshan College | Zhu M.,China Meat Research Center | Yang Y.,Beijing Municipal Institute of Labour Protection | Kang A.,Hebei Chemical and Phamaceutical Vacational Technology College
Advanced Materials Research | Year: 2011

Taking high concentration livestock wastewater as research object, The results showed that the constructed rapid infiltration system operated at the largest hydraulic loading of 0.26m/d, and during the period of the flooding for ond day and drying for two days, the main efficiency of pollutants occurred in filter height of 0.65m, with the maximum removal efficiency of COD and ammonia nitrogen as 80%; TF and MLVSS also reached maximum. © (2011) Trans Tech Publications, Switzerland. Source

Zhang J.,China Meat Research Center | Zhao J.,Beijing Academy of Food science | Zhao Y.,Beijing Academy of Food science | Yang C.,Beijing Academy of Food science | Wang W.,Beijing Academy of Food science
Resources, Environment and Engineering - 2nd Technical Congress on Resources, Environment and Engineering, CREE 2015 | Year: 2016

To reveal the biochemical characteristics, proteins in Soy Sauce Residues (SSR) was prepared by centrifugation in combination with lyophilization. The proteins in SSR were identified by SDS-PAGE and MALDI-TOF-MS. Results showed that acidic polypeptides (A1a, A2, A1b, A4) and basic polypeptides (B2, B1a, B1b, B3, B4) of glycinin from soybean proteins were the predominant proteins in SSR. In order to make better use of the residual protein in SSR, the optimal conditions of the cellulase and alcalase were analyzed. The optimal conditions for two-step enzymatic hydrolysis of soy sauce residue were as follows: pre-treatment by 0.01% Celluclast 1.5 L at pH 5.0, 55°C for 1 h, together with treatment by 0.05% Alacase 2.4 L at pH 8.0, 65°C for 2 h. The hydrolysis yield of protein (Soluble nitrogen index) could reach 62.45%. The analysis of the crude protein dissolution rate indicated that enzyme combinations could hydrolyze the proteins of SSR effectively. © 2016 Taylor & Francis Group, London. Source

Wei M.,Water Resources University | Yuan F.,H+ Technology | Huang G.,China Meat Research Center | Chen H.,Water Resources University | Liu F.,Water Resources University
Environmental Science and Pollution Research | Year: 2016

Lab-scale parallel continuous-flow column experiments were performed to assess the long-term effect of nitrate (NO3 −) on hexavalent chromium (Cr(VI)) removal by scrap iron (Fe0). The first column (L1) was fed with the Cr(VI) solution and the second column (L2) was loaded with the Cr(VI) + NO3 − solution. Raman spectroscopy and scanning electron microscopy energy-dispersive X-ray analyses (SEM-EDS) were conducted to investigate the changes of the iron oxides on Fe0. The results showed that the process of Cr(VI) removal by Fe0 was divided into three different stages in the presence of NO3 −: inhibition period (<198 pore volumes (PVs)); promotion period (198∼1025 PVs); and complete passivation period (1025∼1300 PVs). During the 462∼1025 PVs, Cr(VI) removal capacity in L2 was about 2.5 times higher than that in L1, and the longevity of L2 than L1 was 275PVs longer. NO3 − exhibited the most dominant effect on the Cr(VI) removal by Fe0 in the last two stages. New magnetite (Fe3O4) produced by the redox reaction of NO3 − and Fe0 was discovered on the surface of the Fe0 obtained from L2. The new generated Fe3O4 could directly reduce the Cr(VI) and could also act as an inhibitor for the formation of passive film on the Fe0 surface as well as an electron mediator that facilitated electron transport from Fe0 to adsorbed Cr(VI). © 2016 Springer-Verlag Berlin Heidelberg Source

Kong X.,Institute of Hydrogeology and Environmental Geology | Liu F.,Water Resources University | Huang G.,China Meat Research Center
Chinese Journal of Environmental Engineering | Year: 2014

This paper described a multi-material permeable reactive barriers for the ammonium-contaminated water remediation, using abiotic ion exchange and microbial degradation processes (nitrification and denitrification). A sequential setup combining different reactive materials and removal processes was designed in the laboratory-scale column test. The results showed that ammonium and its products (nitrite & nitrate) were removed to levels below the regulatory discharge limits, under the conditions of the flow velocity of 0.5 m/d and influent ammonium concentration of 10 mg/L. Oxygen releasing materials could supply enough oxygen for microbial nitrification. The concentration of dissolved oxygen in groundwater increased from 2 mg/L to 10 mg/L. Ammonium was efficiently removed in the first microbial nitrification column filled with bio-ceramsite and zeolite, in which 50% of the ammonium was removed through the biological process. The subsequent microbial denitrification columns filled with sponge iron and pine bark were used to remove nitrate formed in microbial nitrification compartment. Sponge iron could provide anaerobic environment, pine bark dissolved slowly in water and supplied enough carbon for the microbial growth. The ammonium concentration decreased from 10 mg/L to 5 mg/L after flowing through the anaerobic column, realizing the effective removal of nitrate formed in the first nitration compartment and avoiding the groundwater secondary pollution. Source

Xie N.,China Agricultural University | Xie N.,Key Laboratory of Functional Dairy | Zhou T.,China Agricultural University | Zhou T.,China Meat Research Center | And 2 more authors.
Food Research International | Year: 2012

This study investigated pure Lactobacillus paracasei H9 tolerance to simulated gastrointestinal juices and adhesion to intestinal mucosa cells without yeasts, with viable yeasts (VY) and with different pretreated yeasts. Three models including gastric secretion tolerance (GST), intestinal juice tolerance (IJT) and sequential gastrointestinal tolerance (SGT) were respectively employed to assay the tolerance of L. paracasei H9, whilst Caco-2 cell line was used to investigate the bacterial adhesion. Particularly, the co-aggregation ability of the two strains at pH values of 2.0, 8.0 and 7.2 was originally carried out to study relations to the bacterial probiotic potentials. Results showed that yeast counts in the range from 3.0 to 5.0 log CFUmL -1 could gradually increase the viability of L. paracasei H9 in SGT. The bacterial viability in the three tolerance models and the adherent number to Caco-2 cells were significantly improved with addition of VY (P<0.05). The L. paracasei H9 with VY in gastric juice at pH 2.0 and intestinal juice at pH 8.0, respectively, exhibited higher aggregation percentage compared with that of single L. paracasei H9 at 37°C (P<0.05). The aggregation ability of L. paracasei H9 with VY at pH 7.2, which might contribute to increase the adhesion of the bacteria, also excelled that of L. paracasei H9 (P<0.05). It is deduced that proteins of the bacterial cell surface and polysaccharides in yeast cell walls play important roles in co-aggregation of the two strains and the microbial adhesion specificity to Caco-2 cells. The co-aggregation of the two strains also contributes to enhancing probiotic potentials of L. paracasei H9. © 2011 Elsevier Ltd. Source

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