China Education Ministrys Key Laboratory In Agricultural Animal Genetics

Wuhan, China

China Education Ministrys Key Laboratory In Agricultural Animal Genetics

Wuhan, China
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Sun L.,China Education Ministrys Key Laboratory in Agricultural Animal Genetics | Sun L.,Huazhong Agricultural University | Song Y.,Huazhong Agricultural University | Riaz H.,China Education Ministrys Key Laboratory in Agricultural Animal Genetics | And 8 more authors.
Veterinary Immunology and Immunopathology | Year: 2012

Toll-like receptors (TLRs) are a class of pattern recognition receptors that play a pivotal role in the innate and adaptive immune systems. Studies have shown that TLR variants play roles in various human infectious diseases. The aim of the present study was to investigate whether the functional genetic variations at positions C632T, G1409A, A1475C, G1550A and G1596A in TLR1 and at A2700G and A3156G in TLR9 confer susceptibility or resistance to bovine tuberculosis (bTB). Genotyping of the TLR1 and TLR9 gene polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-strand conformation polymorphism (PCR-SSCP) in 586 Chinese Holstein cows (154 infected with bTB, 432 non-infected). The frequencies of the GH and HH genotypes at TLR1-G1596A differed significantly between bTB-infected and non-infected animals [p= 0.001 for both GH and HH; GH odds ratio (OR) = 2.43 95% confidence interval (CI) (1.47-4.03); and HH OR = 1.49 95% CI (0.85-2.62)]. There was a trend toward an increased relative risk of tuberculosis (TB) incidence in the CD genotype at the TLR1-A1475C locus [p= 0.056, OR = 1.59 95% CI (0.98-2.58)]. The present study suggests that variants in the TLR1 gene are associated with susceptibility to bTB, whereas no significant association can be inferred from the polymorphisms in the TLR9 gene. © 2012 Elsevier B.V.


Sun L.P.,China Education Ministrys Key Laboratory In Agricultural Animal Genetics | Sun L.P.,Zhengzhou University of Light Industry | Song Y.P.,Huazhong Agricultural University | Liu J.J.,China Education Ministrys Key Laboratory In Agricultural Animal Genetics | And 3 more authors.
Genetics and Molecular Research | Year: 2015

Spermatogenesis, is a complex process of precisely regulated intracellular events, where it is affected by many factors. Long-distance transport of animals is one of the stressors that may influence spermatogenesis and sperm quality. The present study chose luteinizing hormone receptor (LHR), androgen receptor (AR), and heat-shock protein 70 (HSP70) as our target genes to investigate their mRNA and protein expression in the testes of long-distance transported (about 1000 km) mice. Histological analysis showed that there was a reduction in the thickness of the seminiferous epithelium in the transported mice, and a significant decrease in body weight and sperm count in the epididymis was also observed. mRNA expression was determined by QPCR in the testis of transported and control mice. The levels for AR decreased significantly in transported mice. LHR and HSP70 expression in the testes of the transported mice was slightly higher than that of control mice but did not reach a significant level. A similar tendency of protein expression was also observed by Western blot analysis. The levels of LHR and HSP70 increased slightly after transportation. However, none of the changes were statistically significant compared with the control mice. In conclusion, long-distance transport has an adverse effect on reproductive organs and spermatozoa in adult mice. © FUNPEC-RP.


Song Y.,Huazhong Agricultural University | Sun L.,Zhengzhou University of Light Industry | Sun L.,China Education Ministrys Key Laboratory in Agricultural Animal Genetics | Guo A.,Huazhong Agricultural University | Yang L.,China Education Ministrys Key Laboratory in Agricultural Animal Genetics
Acta Histochemica | Year: 2014

Our present study aimed to investigate the effect of four SNPs (G1793A, C1859A, A1980G, G1934A) in toll-like receptor 6 (TLR6) on bovine tuberculosis (bTB) resistance in a case-control study. A total of 603 Chinese Holstein cattle (264 from a dairy farm of Henan province, 339 from Hubei province) were selected to analyze the genotype of TLR6 gene by PCR-RFLP. Genotype frequencies of C1859A and A1980G site differed significantly between bTB-infected and non-infected cows (χ2=6.062, P=0.048 and χ2=6.749, P=0.034, respectively). Relative risk of tuberculosis incidence result showed that genotypes of AA or CA had greater relative risk (OR=2.730, 95%CI=0.869-8.573; OR=1.547, 95CI%=0.803-2.982, respectively) than those with genotype CC at C1859A site between bTB-infected and non-infected animals. Genotypes of GG or GA had greater relative risk (OR=2.986, 95%CI=1.245-7.165; OR=1.582, 95%CI=0.734-3.409, respectively) than those with genotype AA at A1980G site. No significant association can be inferred from G1793A and G1934A polymorphism site. The present study suggests that variants in the TLR6 gene are associated with susceptibility to bTB and the TLR6 gene may be considered as a candidate gene for bTB resistance. © 2014 Elsevier GmbH.


Yapan S.,Huazhong Agricultural University | Liping S.,Zhengzhou University of Light Industry | Liping S.,China Education Ministrys Key Laboratory in Agricultural Animal Genetics | Aizhen G.,Huazhong Agricultural University | Liguo Y.,China Education Ministrys Key Laboratory in Agricultural Animal Genetics
Livestock Science | Year: 2014

Toll-like receptor 2 (TLR2) is an important pattern recognition receptor that play a pivotal role in the innate and adaptive immune systems. TLR2 has been identified as susceptibility loci for various infectious diseases. The aim of the present study was to investigate whether the functional genetic variations in TLR2 gene (T385G, G398A and C1828T) confer susceptibility or resistance to bovine brucellosis. The variants of TLR2 gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 511 Chinese Holstein cows (143 infected with brucellosis, 368 non-infected). For SNP T385G, genotypic frequency of GT and GG were significantly difference between brucella-infected and non-infected animals [. p=0.0401 for both GT and GG; GT Odds Ratio (OR)=1.410 95% confidence interval (CI) (0.923-2.155); and GG OR=2.084 95% CI (1.116-3.893)]. Similarly, G allele showed significantly higher relative risk of brucellosis incidence than T allele [. p=0.0064, OR=1.519 95% CI (1.123-2.053)]. The present study indicates that TLR2 gene could be a potential genetic marker for bovine brucellosis susceptibility. © 2014 Elsevier B.V.


PubMed | Huazhong Agricultural University, China Education Ministrys Key Laboratory in Agricultural Animal Genetics and Zhengzhou University of Light Industry
Type: Journal Article | Journal: Acta histochemica | Year: 2014

Our present study aimed to investigate the effect of four SNPs (G1793A, C1859A, A1980G, G1934A) in toll-like receptor 6 (TLR6) on bovine tuberculosis (bTB) resistance in a case-control study. A total of 603 Chinese Holstein cattle (264 from a dairy farm of Henan province, 339 from Hubei province) were selected to analyze the genotype of TLR6 gene by PCR-RFLP. Genotype frequencies of C1859A and A1980G site differed significantly between bTB-infected and non-infected cows ((2)=6.062, P=0.048 and (2)=6.749, P=0.034, respectively). Relative risk of tuberculosis incidence result showed that genotypes of AA or CA had greater relative risk (OR=2.730, 95%CI=0.869-8.573; OR=1.547, 95CI%=0.803-2.982, respectively) than those with genotype CC at C1859A site between bTB-infected and non-infected animals. Genotypes of GG or GA had greater relative risk (OR=2.986, 95%CI=1.245-7.165; OR=1.582, 95%CI=0.734-3.409, respectively) than those with genotype AA at A1980G site. No significant association can be inferred from G1793A and G1934A polymorphism site. The present study suggests that variants in the TLR6 gene are associated with susceptibility to bTB and the TLR6 gene may be considered as a candidate gene for bTB resistance.


PubMed | China Education Ministrys Key Laboratory in Agricultural Animal Genetics
Type: Journal Article | Journal: Veterinary immunology and immunopathology | Year: 2012

Toll-like receptors (TLRs) are a class of pattern recognition receptors that play a pivotal role in the innate and adaptive immune systems. Studies have shown that TLR variants play roles in various human infectious diseases. The aim of the present study was to investigate whether the functional genetic variations at positions C632T, G1409A, A1475C, G1550A and G1596A in TLR1 and at A2700G and A3156G in TLR9 confer susceptibility or resistance to bovine tuberculosis (bTB). Genotyping of the TLR1 and TLR9 gene polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-strand conformation polymorphism (PCR-SSCP) in 586 Chinese Holstein cows (154 infected with bTB, 432 non-infected). The frequencies of the GH and HH genotypes at TLR1-G1596A differed significantly between bTB-infected and non-infected animals [p=0.001 for both GH and HH; GH odds ratio (OR)=2.43 95% confidence interval (CI) (1.47-4.03); and HH OR=1.49 95% CI (0.85-2.62)]. There was a trend toward an increased relative risk of tuberculosis (TB) incidence in the CD genotype at the TLR1-A1475C locus [p=0.056, OR=1.59 95% CI (0.98-2.58)]. The present study suggests that variants in the TLR1 gene are associated with susceptibility to bTB, whereas no significant association can be inferred from the polymorphisms in the TLR9 gene.


PubMed | China Education Ministrys Key Laboratory in Agricultural Animal Genetics and Huazhong Agricultural University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Spermatogenesis, is a complex process of precisely regulated intracellular events, where it is affected by many factors. Long-distance transport of animals is one of the stressors that may influence spermatogenesis and sperm quality. The present study chose luteinizing hormone receptor (LHR), androgen receptor (AR), and heat-shock protein 70 (HSP70) as our target genes to investigate their mRNA and protein expression in the testes of long-distance transported (about 1000 km) mice. Histological analysis showed that there was a reduction in the thickness of the seminiferous epithelium in the transported mice, and a significant decrease in body weight and sperm count in the epididymis was also observed. mRNA expression was determined by QPCR in the testis of transported and control mice. The levels for AR decreased significantly in transported mice. LHR and HSP70 expression in the testes of the transported mice was slightly higher than that of control mice but did not reach a significant level. A similar tendency of protein expression was also observed by Western blot analysis. The levels of LHR and HSP70 increased slightly after transportation. However, none of the changes were statistically significant compared with the control mice. In conclusion, long-distance transport has an adverse effect on reproductive organs and spermatozoa in adult mice.

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