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Han X.,Shandong University of Technology | Zhang B.,China Aeronautical Project and Design Institute | Li S.,Beihang University | Zhang D.,Beihang University
Nongye Jixie Xuebao/Transactions of the Chinese Society of Agricultural Machinery | Year: 2011

Focusing on the problems existing in the biochip microarray manufacturing methods such as low maintenance in bioactivity, microjet head blockage and high consumption in biological reagent and so on, the ultrasonic transducer and spherical acoustic lens based on ultrasonic focusing microjet head as well as the downward isolated jetting mechanism was designed. Based on this, no nozzle, non blockage, non contact, low consumption and high accuracy was gained in the microarray manufacture. To verify the biocompatibility of the microjet system, the F0F1-ATP molecular-motor assembled liposome microarrays with regular shape and accurate location was prepared. Furthermore, the ATP hydrolysis activity and the ATP synthesis activity of the liposome microarrays were tested respectively. Experimental results indicated that the liposome microarrays fabricated this way showed favorable ATP hydrolysis activity and synthesis activity, which confirmed that the ultrasonic focusing microjet method has favorable biocompatibility in manufacturing biological microarrays. The investigation elaborated here explored a new manufacturing approach for biological microarrays. Source

Cui Y.,Beijing University of Technology | Ding J.,Beijing University of Technology | Li J.,China Aeronautical Project and Design Institute | Chen Y.,Beijing University of Technology | Su H.,Beijing University of Technology
Yingyong Jichu yu Gongcheng Kexue Xuebao/Journal of Basic Science and Engineering | Year: 2012

Halophilic activated sludge was developed by seeding with sediment of the estuary to the sea. During 120d operation, stable short-cut nitrification was achieved in SBRs treating saline domestic wastewater containing 30±2g/L salts. Based on the short-cut nitrification theory of fresh activated sludge, this study evaluated some key process factors possibly contributing to nitrite accumulation such as pH, free ammonium(FA), temperature, dissolved oxygen(DO)and aeration time. The results showed the optimal pH and salinity of halophilic activated sludge was 7. 5-9g/L and 10-61g/L, respectively. Aeration time and FA was not the reasons inducing high nitrite accumulation. Salinity and temperature have impact on both the ammonium oxidizing bacteria(AOB)and the nitrite oxidizing bacteria(NOB). However, the activity of halophilic AOB remained higher than halophilic NOB during the experiment. Fluorescence in situ hybridization(FISH)for visualizing nitrification community of the sediments and the halophilic activated sludge showed that AOB was the dominant nitrifiers in the both biological systems. The high nitrite accumulation in halophilic activated sludge may be owed to two reasons, one is that halophilic AOB has higher substrate degrading rate than that of halophilic NOB, the other is that the dominant bacterial population is halophilic AOB other than halophilic NOB. Source

Cui Y.,Beijing University of Technology | Ding J.,Beijing University of Technology | Li J.,China Aeronautical Project and Design Institute | Su H.,Beijing University of Technology | Chen Y.,Beijing University of Technology
Huagong Xuebao/CIESC Journal | Year: 2011

Due to effects of high osmotic pressure and biological toxicity of inorganic salts, eubolism of activated sludge developing in fresh wastewater can not be carried out in highly saline wastewater. This difficulty could be solved by collecting, studying and culturing halophilic activated sludge from the estuary deposit at the mouth of the river. In this paper the effect of start-up, ammonium-nitrogen loading and salinity on nitrification performance of the halophilic nitrification culture in treating real saline domestic wastewater was studied. The results show that after 9 day adjustment stage, nitrification of ammonium-nitrogen is started. This halophilic system is sensitive to ammonium-nitrogen loading and its fluctuation. Salt tolerance of 10-60 g·L -1 as well as optimum salt of about 40 g·L -1 was established. Comparing with fresh activated sludge and salt-acclimation activated sludge, this system is of advantages of high specific ammonium removal rate and tolerance of salt shocks, because the dominant population is composed by moderately halophilic and marine bacteria. A noteworthy is that there is unusually high nitrite accumulation rate during experimental operation although all operation conditions are very preferable to nitrite oxidation. The research can provide an alternative way for application of the halophilic mixed culture in biological treatment of highly saline wastewater. © All Rights Reserved. Source

Hao G.-K.,Northwest University, China | He W.-P.,Northwest University, China | Yan H.,China Aeronautical Project and Design Institute | Zhao F.,Xian University of Architecture and Technology
Jisuanji Jicheng Zhizao Xitong/Computer Integrated Manufacturing Systems, CIMS | Year: 2010

To deal with low efficiency and difficult management of the reconfiguration of component-based manufacturing execution system (MES), a model-driven engineering method was proposed. The reconfiguration was realized from life-cycle perspective and a higher abstraction level. The concept of reconfigurable asset was introduced and its meta-model was designed. A domain modeling language oriented to MES was established to describe requirements and decisions of the reconfiguration. Model transformation and storage technology of the reconfiguration process were also introduced. Finally the method was validated in an aerospace enterprise. Source

Sun G.,Nanjing Southeast University | Li A.,Nanjing Southeast University | Huang Z.,Nanjing Southeast University | Li Z.,Nanjing Southeast University | And 5 more authors.
Zhendong Ceshi Yu Zhenduan/Journal of Vibration, Measurement and Diagnosis | Year: 2010

The dynamic characteristic of the AMECO-A380 maintenance hangar in Beijing capital airport was field-tested excited by environmental random loads. The test data were analyzed by using the spectrum analysis and the natural frequency and the mode of structure were obtained. Based on a finite element model of the hangar structure, the parameters of structural dynamic characteristic were calculated and compared with the measured values. The analysis shows that the test result of fundamental natural frequency agrees well with the finite element calculation result as well as the first mode shape respectively. Source

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