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Zhang H.,Childrens Hospital of Xuzhou
Chinese Journal of Cardiology | Year: 2014

Objective: To explore [he effects of astragalus (AST), total flavone of astragalus (TFA), total saponins of astragalus (TSA) and astragalus polysaccharides (APS) on ischemia/reperfusion (40 min/60 min) injury in isolated guinea-pig heart.Methods: Isolated guinea-pig hearts underwent ischemia, then followed by K-H perfusion (I/R group), AST (60 mg/L), AST (60 mg/L),TFA (60 mg/L),TSA (60 mg/L) and APS (60 mg/L) perfusion (n =6 each). Isolated hearts without ischemia serve as control group (n = 6). Activity of lactate dehydrogenas (LDH) and creatine kinase (CK) in effluent were measured. Infarct size, myocardial superoxide dismutase (SOD) activity and malondiadehyde (MDA) contents were also determined.Results: Compared to control hearts,heart rate,coronary flow and myocardial superoxide dismutase (SOD) activity were significantly reduced,while LDH and CK in effluent as well as myocardial MDA were significantly increased in the 1/R hearts during reperfusion (all P < 0. 05), these changes could be partly reversed by AST and TFA perfusion. Infarct size was also significantly reduced in AST (1 1. 9 ±2. 03)% and TFA (13. 31 ±1. 17)% treated hearts compared to that in I/R group(18. 9 ± 2. 27) % (all P < 0. 01).Conclusions: The findings indicate that AST and TFA could attenuate I/R injury in isolated guinea-pig heart possibly through enhancing the activity of SOD and reducing lipid peroxidation. Source


Zhang H.,Childrens Hospital of Xuzhou | Min D.,Childrens Hospital of Xuzhou | Fu M.,Childrens Hospital of Xuzhou | Tian J.,Childrens Hospital of Xuzhou | And 2 more authors.
Zhonghua xin xue guan bing za zhi | Year: 2014

OBJECTIVE: To explore the effects of astragalus (AST) , total flavone of astragalus (TFA), total saponins of astragalus (TSA) and astragalus polysaccharides (APS) on ischemia/reperfusion (40 min/60 min) injury in isolated guinea-pig heart.METHODS: Isolated guinea-pig hearts underwent ischemia, then followed by K-H perfusion (I/R group), AST (60 mg/L),AST (60 mg/L), TFA (60 mg/L), TSA (60 mg/L) and APS (60 mg/L) perfusion (n = 6 each).Isolated hearts without ischemia serve as control group (n = 6). Activity of lactate dehydrogenas (LDH) and creatine kinase (CK) in effluent were measured.Infarct size, myocardial superoxide dismutase (SOD) activity and malondiadehyde (MDA) contents were also determined.RESULTS: Compared to control hearts, heart rate, coronary flow and myocardial superoxide dismutase (SOD) activity were significantly reduced, while LDH and CK in effluent as well as myocardial MDA were significantly increased in the I/R hearts during reperfusion (all P < 0.05), these changes could be partly reversed by AST and TFA perfusion.Infarct size was also significantly reduced in AST (11.9 ± 2.03) % and TFA (13.31 ± 1.17) % treated hearts compared to that in I/R group (18.9 ± 2.27) % (all P < 0.01).CONCLUSIONS: The findings indicate that AST and TFA could attenuate I/R injury in isolated guinea-pig heart possibly through enhancing the activity of SOD and reducing lipid peroxidation. Source


Zhuo B.,Childrens Hospital of Xuzhou | Li Y.,Childrens Hospital of Xuzhou | Li Z.,Childrens Hospital of Xuzhou | Qin H.,Childrens Hospital of Xuzhou | And 5 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

Accumulating evidence has shown that PI3K/Akt pathway is frequently hyperactivated in osteosarcoma (OS) and contributes to tumor initiation and progression. Altered phenotype of glucose metabolism is a key hallmark of cancer cells including OS. However, the relationship between PI3K/Akt pathway and glucose metabolism in OS remains largely unexplored. In this study, we showed that elevated Hexokinase-2 (HK2) expression, which catalyzes the first essential step of glucose metabolism by conversion of glucose into glucose-6-phosphate, was induced by activated PI3K/Akt signaling. Immunohistochemical analysis showed that HK2 was overexpressed in 83.3% (25/30) specimens detected and was closely correlated with Ki67, a cell proliferation index. Silencing of endogenous HK2 resulted in decreased aerobic glycolysis as demonstrated by reduced glucose consumption and lactate production. Inhibition of PI3K/Akt signaling also suppressed aerobic glycolysis and this effect can be reversed by reintroduction of HK2. Furthermore, knockdown of HK2 led to increased cell apoptosis and reduced ability of colony formation; meanwhile, these effects were blocked by 2-Deoxy-d-glucose (2-DG), a glycolysis inhibitor through its actions on hexokinase, indicating that HK2 functions in cell apoptosis and growth were mediated by altered aerobic glycolysis. Taken together, our study reveals a novel relationship between PI3K/Akt signaling and aerobic glycolysis and indicates that PI3K/Akt/HK2 might be potential therapeutic approaches for OS. © 2015 Elsevier Inc. All rights reserved. Source


Zhuo B.-B.,Childrens Hospital of Xuzhou | Zhang H.-W.,Childrens Hospital of Xuzhou | Liu F.-L.,Childrens Hospital of Xuzhou | Ma T.-S.,Childrens Hospital of Xuzhou | And 2 more authors.
National Medical Journal of China | Year: 2012

Objective: To explore the efficacies of treating infants with congenital anorectal malformation by drawing from rectal muscle sheath of blind bag out of previous sagittal approach (modified Mollard procedure). Methods: Retrospective analyses of postoperative anus control and bowel movements were conducted for 172 patients with high anorectal malformation. The procedures included modified Mollard (n=68, modified group), Pena (n=64, Pena group) and abdominal perineal anus forming (n=40, abdominoperineal group). The tensions of external sphincter and puborectalis were gauged by digital rectal examination and the perianal degree of fecal pollution was assessed by defecography. Results: Among them, 28 boys and 18 girls had a good postoperative control of defecation in the modified group (P=0.004). The ratios of postoperative external anal sphincter was strong were 73.5% (50/68) and 85.9% (55/64) respectively in the modified and Pena groups and they were higher than that of abdominal perineal group at 55.0% (22/40) (both P < 0.05). The difference in the former two groups was not statistically significant (P=0.196). The incidence of constipation in the modified group was less than that in the Pena group (13.2% (9/68) vs 31.3% (20/64), P=0.012). Conclusion: Modified Mollard procedure may avoid repeated operations, offer a better control of bowel function, ease patient suffering and improve their postoperative quality-of-life. Source

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