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Cao J.,Chongqing Medical University | Dai D.-L.,Childrens Hospital of Shenzhen | Yao L.,Central Hospital of Jiangjin | Yu H.-H.,Chongqing Medical University | And 6 more authors.
Molecular and Cellular Biochemistry | Year: 2012

Accumulation of saturated fatty acids in the liver can cause nonalcoholic fatty liver disease (NAFLD). This study investigated saturated fatty acid induction of endoplasmic reticulum (ER) stress and apoptosis in human liver cells and the underlying causal mechanism. Human liver L02 and HepG2 cell lines were exposed to the saturated fatty acid sodium palmitate. MTT assay was used for cell viability, flow cytometry and Hoechst 33258 staining for apoptosis, RT-PCR for mRNA expression, and Western blot for protein expression. Silence of PRK-like ER kinase (PERK) expression in liver cells was through transient transfection of PERK shRNA. Treatment of L02 and HepG2 cells with sodium palmitate reduced cell viability through induction of apoptosis. Sodium palmitate also induced ER stress in the cells, indicated by upregulation of PERK phosphorylation and expression of BiP, ATF4, and CHOP proteins. Sodium palmitate had little effect on activating XBP-1, a common target of the other two canonical sensors of ER stress, ATF6, and IRE1. Knockdown of PERK gene expression suppressed the PERK/ATF4/CHOP signaling pathway during sodium palmitateinduced ER stress and significantly inhibited sodium palmitate-induced apoptosis in L02 and HepG2 cells. Saturated fatty acid-induced ER stress and apoptosis in these human liver cells were enacted through the PERK/ATF4/CHOP signaling pathway. Future study is warranted to investigate the role of these proteins in mediating saturated fatty acid-induced NAFLD in animal models. © Springer Science+Business Media, LLC. 2011.


Tong Y.,University of Science and Technology of China | Sun H.,University of Science and Technology of China | Luo Q.,University of Science and Technology of China | Feng J.,Childrens Hospital of Shenzhen | And 7 more authors.
Biological Trace Element Research | Year: 2011

In order to study the daily Pb absorption in fetus and to monitor the main Pb sources in prenatal fetus, we have investigated several cases of Pb distribution along the longitudinal axis of fetal hair. The changes of Pb levels in the pregnancy period, even the daily changes of Pb levels can be detected in the hair. Therefore, by analyzing the Pb distribution curves in the fetal hair and the living habits of their mothers, the main sources of Pb in the prenatal fetus can be evaluated. In our study, the main sources of Pb in the two cases of prenatal fetus studied here should be from the polluted aquatics. © The Author(s) 2010.


Tong Y.,Shenzhen University | Hao X.,Shenzhen University | Sun H.,Shenzhen University | Feng J.,Childrens Hospital of Shenzhen | And 2 more authors.
Shenzhen Daxue Xuebao (Ligong Ban)/Journal of Shenzhen University Science and Engineering | Year: 2014

The concentration of 50 cases of coastal city fetal umbilical cord blood lead (Pb) level was analyzed by inductively coupled plasma mass spectrometry (ICP-MS). The results showed that about 8% of them exceeded the current blood lead standard (> 100 μg/L). Meanwhile, the 50 cases were divided into three groups as such: regular consumption of seafood (group A), occasional consumption of seafood (group B), and rare consumption of seafood (group C) for analysis, respectively. The mass fraction of lead from fetal hair was analyzed by synchrotron radiation micro-XRF analysis. The results showed that lead content was significantly different in the groups and the trend of baby hair Pb content was as A > B > C group. The corresponding cord blood Pb levels also have the same trend. Bromine (Br) content was also measured and the result showed that the group with high level of Pb also had a high level of Br. As Br content is much higher in seafood than in freshwater products, the high Pb content with high Br content may be associated with the consumption of seafood. At the same time, blood Pb levels of 393 cases of children (from 2 to 11 years old) in the same coastal city were statistically analyzed, and the result showed that 8.9% of them exceeded the current blood lead standard (> 100 μg/L). The above tests indicate that Pb pollution still threaten the health of children. Finally, through the comparison of common main sources of lead in the environment (PM2.5, air, paint, seafood, etc.) as well as in combination with human intake conditions, it is concluded that lead-contaminated seafood consumption is the main reason of coastal city of fetal lead excess.

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