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Bi X.,Liaoning University | Liu W.,Liaoning University | Xia X.,Liaoning University | Chen L.,Liaoning University | And 4 more authors.
Food and Function

The aim of this study was to investigate the effects of Callistephus chinensis flower (CCF) polyphones on symptoms of metabolic syndrome in a newly developed high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) mouse model. C57BL/6J mice were fed a high fat diet (HFD; 50% energy as fat) with normal drinking fluid or HFD with CCF polyphones (50 mg L-1 or 100 mg L-1) in drinking fluid for 12 weeks. As a comparison, mice fed a normal-fat (NFD; 10% energy as fat) and with normal drinking fluid were also included. The HFD group developed more severe symptoms of metabolic syndrome than the NFD group. CCF polyphones treatment significantly reduced fecal lipids compared to the HFD group, suggesting a strong indication of improved lipid metabolism. Liver damage and liver triglyceride levels were also decreased by CCF polyphones treatment. Moreover, both morphologic and histological detections indicated that CCF polyphones significantly reversed HFD-induced hepatic steatosis and liver injury. Furthermore, CCF polyphones significantly ameliorated both HFD-induced metabolic disorders, such as insulin resistance, and inflammatory cytokines, including interlukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Moreover, hepatic peroxisome proliferator-activated receptor (PPARα) and the gene involved in PPARα, Peroxisomal acyl-CoA oxidase (ACOX), were markedly up-regulated at protein levels by CCF polyphones. Our results demonstrate that the HFD produces metabolic syndrome of NAFLD, and CCF polyphones treatment can alleviate these symptoms. The beneficial effects of CCF polyphones are associated with improved lipid metabolism and reduced levels of inflammatory cytokines. This journal is © 2014 The Royal Society of Chemistry. Source

Zhang X.,Shenyang Pharmaceutical University | Liu Z.,Chifeng MinYi Natural Anthocyanins Company Ltd | Bi X.,Liaoning University | Liu J.,Chifeng MinYi Natural Anthocyanins Company Ltd | And 2 more authors.
EXCLI Journal

Inhibitors of carbohydrate-hydrolysing enzymes play an important role for the treatment of diabetes. One of the therapeutic methods for decreasing of postprandial hyperglycemia is to retard absorption of glucose by the inhibition of carbohydrate- hydrolysing enzymes, such as α-glucosidase, in the digestive organs. To investigate the therapeutic potential of compounds from natural sources, Callistephus chinensis flowers (CCF) were tested for inhibition of α- glucosidase, and acarboes was used as the positive control. The 70 % ethanol extract of CCF exhibited significant α-glucosidase inhibitory activities with IC50 value of 8.14 μg/ml. The stepwise polarity fractions of CCF were tested further for in vitro inhibition of α-glucosidase. The ethyl acetate (EtOAc) fraction exhibited the most significant inhibitory activity. Eight pure compounds, apigenin, apigenin-7-O-β-D- glucoside, kaempferol, hyperin, naringenin, quercetin, luteolin, and kaempferol-7-O-β-D- glucoside, were isolated (using enzyme assayguide fractionation method) from the EtOAc fraction. Among these, quercetin was the most active one (IC50 values 2.04 μg/ml), and it appears that the inhibiting percentages are close to acarbose (IC50 values 2.24 μg/ml), the positive control, on α-glucosidase inhibition. HPLC/UV analysis indicated that the major components of CCF are kaempferol, hyperin and quercetin. The presented results revealed that CCF containing these eight flavonoids could be a useful natural source in the development of a novel α-glucosidase inhibitory agent against diabetic complications. Source

Zhang X.-S.,Shenyang Pharmaceutical University | Cao J.-Q.,Shenyang Pharmaceutical University | Liu Z.-T.,Chifeng MinYi Natural Anthocyanins Company Ltd | Yuan L.,Shenyang Pharmaceutical University | And 4 more authors.
Phytochemistry Letters

Two new compounds callistephus A (1) and callistephus B (2) together with 4,6-dihydroxyphenyl-1-butanone-2-β-d-O-glucopyranoside (3), were isolated and purified from the Callistephus chinensis flower. Their structures were identified by the interpretation of spectroscopic data. X-ray crystallographic and analysis of quantum chemical ECD calculation were applied to determine the absolute configuration. Of them, callistephus A represents a rare sesquiterpene with new carbon skeleton and also its possible biogenesis was proposed. © 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved. Source

Zhang X.-S.,Shenyang Pharmaceutical University | Cao J.-Q.,Shenyang Pharmaceutical University | Shi G.-H.,Jiangxi University of Traditional Chinese Medicine | Liu Z.-T.,Chifeng MinYi Natural Anthocyanins Company Ltd | And 2 more authors.
Natural Product Research

Two new isoaurones derivatives were obtained from Callistephus chinensis flower. Their structures were elucidated on the basis of extensive spectroscopic analyses. Both of the new compounds were evaluated cytotoxic activity. Phytochemical investigation of Callistephus chinensis flower led to the isolation of two new isoaurones derivatives (Z)-4′,4,10-trihydroxy-siamaurone (1) and (E)-4′,4,10-trihydroxy-siamaurone (2). The structures of these new compounds were identified by the interpretation of spectroscopic data (mainly 1D and 2D NMR) and by comparison with data reported in the literature. Both of the new compounds were evaluated for their cytotoxicity. © 2015 Taylor & Francis Source

Shi G.-H.,Jiangxi University of Traditional Chinese Medicine | Shi G.-H.,Shenyang Pharmaceutical University | Yang S.-L.,Jiangxi University of Traditional Chinese Medicine | Zhang X.-S.,Shenyang Pharmaceutical University | And 3 more authors.
Chinese Traditional and Herbal Drugs

Objective: To establish an HPLC method for the simultaneous determination of five flavonnid components in different parts of Callistephus chinensis. Methods: The chromatographic separation was performed on an XUnion C18 column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile and water, at a flow rate of 1 mL/min, and detected at 210 nm. Results: The calibration curve was linear within 2.5-200 mg/L for hyperoside, apigenin-7-O-β-D-glucopyranoside, luteolin, apigenin, and kaempferol, respectively, with the correlation r > 0.999 0. The extraction recoveries varied from 95% to 105%. Conclusion: The method is accurate and selective, and can be used for the quality control of different parts of C. chinensis. ©, 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved. Source

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