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PubMed | Chiatai QingChunbao Pharmaceutical Co., Zhejiang Academy of Medical science and Beijing Space Odyssey Biotechnology Co.
Type: | Journal: Regulatory toxicology and pharmacology : RTP | Year: 2016

SHENMAI injection (SMI), derived from famous Shen Mai San, is a herbal injection widely used in China. Ginsenosides are the major components of SMI. To monitor the exposure level of SMI during long-term treatment, a 6-month toxicokinetic experiment was performed. Twenty-four beagle dogs were dived into four groups (n=6 in each group): a control group (0.9% NaCl solution) and three SMI groups (2, 6 or 3mg/kg). The dogs were i.v. infused with vehicle or SMI daily for 180d. Blood samples for analysis were collected at specific time points as follows: pre-dose (0h); at 10, 30, and 60min during infusion; and at 10, 30, 60, 90, 120, 240, and 300min post-administration. Concentrations of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1 in the plasma were determined simultaneously by liquid chromatography-tandem mass spectrometry. Non-compartmental parameters were further calculated and analyzed. Significant differences were found between the kinetic behavior of 20(S)-protopanaxadiol-type (PPD-type) and 20(S)-protopanaxatriol-type (PPT-type) ginsenosides. Increasing in the exposure level of PPD-type ginsenosides was observed in dogs during the experiment. Therefore, PPD-type ginsenosides are closely related to the immunity modulation effect of SMI. Increased PPD-type ginsenoside exposure level may present potential toxicity and induce drug-drug interaction risks during SMI administration. As such, PPD-type ginsenoside accumulation must be carefully monitored in future SMI research.


Chu C.,Zhejiang University of Technology | Xu S.,Zhejiang University of Technology | Li X.,Zhejiang University of Technology | Yan J.,Zhejiang University of Technology | Liu L.,Chiatai Qingchunbao Pharmaceutical Co.
Journal of Food Science | Year: 2013

Ginseng is a well-known herbal medicine that has been gaining increasingly popularity as a potential chemopreventive agent. In traditional Chinese medicine practice, white ginseng (WG), red ginseng (RG), and dali ginseng (DG) are 3 different ginseng-processed products used for different purposes. Although the morphological appearance and some constituents contained in these ginseng products are similar, their pharmacological activities are significantly different due to the varied types and quantity of ginsenosides in each product. In the present study, a practical method based on rapid liquid chromatography coupled with quadrupole time of flight mass spectrometry (LC-Q-TOF/MS) was developed to identify the chemical profiles of ginsenosides in these 3 ginseng products. The results demonstrated that a total of 55, 53, and 43 compounds were unambiguously assigned or tentatively identified in DG, WG, and RG samples, respectively. The featured compounds are mainly malonyl ginsenosides in WG, and decarboxyl products of mal-ginsenosides and the dehydrated compounds from polar ginsenosides were characteristic in RG, while DG contain some characteristic components present both in WG and RG. We presume that heating processing is the major factor affecting the chemical profile of ginseng products. The difference of chemical information revealed by LC-Q-TOF/MS could be used to discriminate the WG, RG, and DG samples. © 2013 Institute of Food Technologists®.


PubMed | Chiatai QingChunbao Pharmaceutical Co Ltd, Nanjing University and China Pharmaceutical University
Type: | Journal: Acta pharmacologica Sinica | Year: 2016

Shenmai injection (SMI) is a Chinese patent-protected injection, which was mainly made of Red Ginseng and Radix Ophiopogonis and widely used for treating coronary heart disease and tumors by boosting Qi and nourishing Yin. In this study we examined whether SMI could produce direct synergetic effects on the cytoxicity of adriamycin (ADR) and paclitaxel (PTX) in colorectal cancers in vivo and in vitro, and explored the underlying pharmacokinetic mechanisms. BALB/c nude mice with LoVo colon cancer xenografts were intraperitoneally injected with ADR (2 mgkg


Xiong H.,Zhejiang University | Fu Y.,Chiatai Qingchunbao Pharmaceutical Co. | Nie J.,Chiatai Qingchunbao Pharmaceutical Co. | Qu H.,Zhejiang University
Zhongguo Zhongyao Zazhi | Year: 2012

Objective: To establish a method for monitoring the quality of intermediates generated in each working procedure during the production process of traditional Chinese medicine (TCM), in order to ensure the batch-to-batch quality consistency of TCM products. Method: The multistage multivariate statistic quality control (MMSQC) was proposed to monitor production quality of TCMs based on multivariate data analysis technique. Hotelling T2 and SPE were adopted for monitoring the quality of intermediates generated in each working procedure. Danshen injection was taken as the example to introduce the application method of MMSQC. Result: MMSQC can monitor the quality of intermediates generated in multiple working procedures, which is simpler and more accurate compared with single-indicator monitoring method. Conclusion: MMSQC can be popularized to monitor quality of multistage production of TCMs.


Yu X.,Zhejiang University | Mo B.-Q.,Chiatai Qingchunbao Pharmaceutical Co. | Wang Z.,Chiatai Qingchunbao Pharmaceutical Co. | Qu H.-B.,Zhejiang University
Chinese Traditional and Herbal Drugs | Year: 2010

Objective: To develop recovery prediction models for danshensu, salvianolic acid B, and salvianolic acid D in danshen ethanol precipitation. Methods: Recorded the operation parameters of 15 normal batches of danshen ethanol precipitation in one Chinese medicine plant and measured five active component contents in danshen concentrated liquid and danshen ethanol precipitated liquid, the Stepwise-MLR recovery prediction models of dashensu, salvianolic acid B, and salvianolic acid D were developed based on an approach which combined the process parameters and the active component contents in danshen concentrated liquid. The importance of variables in the prediction models was analyzed later. Results: The coefficient correlations of dashensu, salvianolic acid B, and salvianolic acid D recovery prediction models were all above 0.95. Conclusion: The models developed for active component recovery prediction show a good predictability. Models can be used to help manufacturers to understand the ethanol precipitation process and improve the quality control ability of danshen ethanol precipitation.


Wu L.,Zhejiang University | Wang Y.,Zhejiang University | Nie J.,Chiatai Qingchunbao Pharmaceutical Co. | Fan X.,Zhejiang University | Cheng Y.,Zhejiang University
Evidence-based Complementary and Alternative Medicine | Year: 2013

The research of multicomponent drugs, such as in Chinese Medicine, on both mechanism dissection and drug discovery is challenging, especially the approaches to systematically evaluating the efficacy at a molecular level. Here, we presented a network pharmacology-based approach to evaluating the efficacy of multicomponent drugs by genome-wide transcriptional expression data and applied it to Shenmai injection (SHENMAI), a widely used Chinese Medicine composed of red ginseng (RG) and Radix Ophiopogonis (RO) in clinically treating myocardial ischemia (MI) diseases. The disease network, MI network in this case, was constructed by combining the protein-protein interactions (PPI) involved in the MI enriched pathways. The therapeutic efficacy of SHENMAI, RG, and RO was therefore evaluated by a network parameter, namely, network recovery index (NRI), which quantitatively evaluates the overall recovery rate in MI network. The NRI of SHENMAI, RG, and RO were 0.876, 0.494, and 0.269 respectively, which indicated SHENMAI exerts protective effects and the synergistic effect of RG and RO on treating myocardial ischemia disease. The successful application of SHENMAI implied that the proposed network pharmacology-based approach could help researchers to better evaluate a multicomponent drug on a systematic and molecular level. © 2013 Leihong Wu et al.


PubMed | Chiatai Qingchunbao Pharmaceutical Co. and Zhejiang University
Type: | Journal: Journal of pharmaceutical and biomedical analysis | Year: 2016

Zhemaidong (ZMD) and Chuanmaidong (CMD) are the two genuine cultivation areas of Ophiopogonis Radix which has been widely used as a traditional Chinese medicine in China for treating cardiovascular and pulmonary diseases. Differences between ZMD and CMD in chemical constituents and pharmacological effects have been reported, however, the details remain largely unknown. The aim of this study was to comprehensively characterize the chemical composition of Ophiopogonis Radix from the two producing areas and compare their immunomodulatory activities. An approach of HPLC-MS coupled with multivariate statistical analysis was established to reveal the characteristic constituents of ZMD and CMD. Furthermore, the effects of ZMD and CMD on the macrophage phagocytosis and gastrointestinal peristalsis were also determined in zebrafish models for assessing the immunomodulatory activities of these two strains. The results revealed that the chemical constituents of ZMD and CMD were much different from each other, and 19 constituents could be served as chemical markers to distinguish these two strains. Moreover, ZMD showed higher promoting rates in macrophage phagocytosis and gastrointestinal motility than those of CMD, suggesting ZMD might possess better immunomodulatory activities. Taken together, the results generated from this study indicated that the herbs from different producing areas should be evaluated and considered in preparing TCM prescriptions.


Yu J.,Zhejiang Academy of Medical science | Xin Y.-F.,Zhejiang Academy of Medical science | Gu L.-Q.,Zhejiang Academy of Medical science | Gao H.-Y.,Zhejiang Academy of Medical science | And 6 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance 'SHENMAI' injection (SMI) has been widely used in cardioprotection and modulation of the immune system because of its great efficacy. SMI primarily comprises the saponins from Panax ginseng and Ophiopogon japonicas. The profiles of saponins in SMI during long-term toxicokinetics remain unclear. MiR-146a possesses excellent sensitivity as a bio-marker in the innate immunity modification effect of SMI. Aim of the study Is to monitor the exposure level of SMI during a one-month toxicokinetic experiment, an analytical method involving ESI-LC-MS/MS technology was developed to determine 20 (S)-protopanaxadiol-type ginsenoside (Rb1, Rb2, Rc, Rd), 20 (S)-protopanaxatriol-type ginsenoside (Rg1, Re, Rf), oleanolic acid-type ginsenoside (Ro), and ophiopogonin D in rats. The levels of AST, CK, ALT, SOD, GSH-pX, MDA, miR-146a, and ECG were measured to explore the effects of SMI in cardiologic function and immune activity. Results Results show that the levels of AST, CK, and MDA decreased upon the administration of SMI. The level of miR-146a increased upon the administration of SMI dosage. During the administration of SMI, increasing exposure levels of 20 (S)-protopanaxadiol-type ginsenosides were also observed. Conclusion The 20 (S)-protopanaxadiol-type ginsenosides were considered potential PK/TK markers because of their high exposure levels that continuously increased. Oxidative stress was slightly alleviated during the toxicokinetic study. Based on the level of miR-146a, negatively regulated innate immunity was observed. The regulation became more serious with increasing exposure levels of 20 (S)-protopanaxadiol-type ginsenosides. Negatively regulated innate immunity could be induced by long-term administration of SMI (>0.4 g/kg). © 2014 Elsevier Ireland Ltd. All rights reserved.


PubMed | Zhejiang Academy of Medical science and Chiatai QingChunbao Pharmaceutical Co.
Type: Journal Article | Journal: Journal of ethnopharmacology | Year: 2014

SHENMAI injection (SMI) has been widely used in cardioprotection and modulation of the immune system because of its great efficacy. SMI primarily comprises the saponins from Panax ginseng and Ophiopogon japonicas. The profiles of saponins in SMI during long-term toxicokinetics remain unclear. MiR-146a possesses excellent sensitivity as a bio-marker in the innate immunity modification effect of SMI.Is to monitor the exposure level of SMI during a one-month toxicokinetic experiment, an analytical method involving ESI-LC-MS/MS technology was developed to determine 20 (S)-protopanaxadiol-type ginsenoside (Rb1, Rb2, Rc, Rd), 20 (S)-protopanaxatriol-type ginsenoside (Rg1, Re, Rf), oleanolic acid-type ginsenoside (Ro), and ophiopogonin D in rats. The levels of AST, CK, ALT, SOD, GSH-pX, MDA, miR-146a, and ECG were measured to explore the effects of SMI in cardiologic function and immune activity.Results show that the levels of AST, CK, and MDA decreased upon the administration of SMI. The level of miR-146a increased upon the administration of SMI dosage. During the administration of SMI, increasing exposure levels of 20 (S)-protopanaxadiol-type ginsenosides were also observed.The 20 (S)-protopanaxadiol-type ginsenosides were considered potential PK/TK markers because of their high exposure levels that continuously increased. Oxidative stress was slightly alleviated during the toxicokinetic study. Based on the level of miR-146a, negatively regulated innate immunity was observed. The regulation became more serious with increasing exposure levels of 20 (S)-protopanaxadiol-type ginsenosides. Negatively regulated innate immunity could be induced by long-term administration of SMI (>0.4g/kg).


Trademark
CHIATAI QINGCHUNBAO PHARMACEUTICAL Co. | Date: 2012-05-15

Chinese herbal teas for medicinal purposes, Chinese herbal-based pharmaceutical tablets and liquids for promoting general well being and anti aging; medicinal tonics for nourishing Yin and tonify Qi, tranquillizing mind and pacify heart, which helps to reduce mental fatigue and weakness in body, caused by deficiency in Yin and Qi; Medicinal tonics for nourishing Yin and tonify Qi, tranquillizing mind and pacify heart, which helps avoid palpitation and short breath, dizziness and vertigo, daily recurring fever and excessive sweating, tinnitus and amnesia, fidgetiness and insomnia caused by deficiency in Yin and Qi.

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