Chhattishgarh Kamdhenu Vishwavidyalaya

Durg, India

Chhattishgarh Kamdhenu Vishwavidyalaya

Durg, India

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Sonar B.P.,Chhattishgarh Kamdhenu Vishwavidyalaya | Tiwari R.P.,Chhattishgarh Kamdhenu Vishwavidyalaya | Poyam M.R.,Chhattishgarh Kamdhenu Vishwavidyalaya | Mishra G.K.,Chhattishgarh Kamdhenu Vishwavidyalaya | And 3 more authors.
Indian Journal of Animal Sciences | Year: 2016

The present research was undertaken to study the characteristics of fresh and cryo-preserved semen of elite pure breed Gir (Bos indicus) bulls. The mean values of fresh seminal parameters in neat semen viz. seminal volume (ml), sperm concentration (millions/ml), progressive sperm motility (%), live sperm (%), intact acrosome (%), total morphological sperm abnormalities (%), hypo osmotic swelling (HOS %) and sperm penetration distance (SPD-mm) were 4.99 ± 0.26, 895.33 ± 82.68, 69.10 ± 0.75, 72.16 ± 0.64, 84.42 ± 0.77, 15.96 ± 0.44, 60.12 ± 1.19 and 31.32 ± 0.70, respectively. Sperm concentration, individual motility, live sperm, total sperm abnormalities and sperm penetration distance differed significantly between bulls. The semen was extended, filled and sealed in 0.25 ml straws maintaining 20 million spermatozoa/straw and cryo-preserved using programmable bio freezer (IMV). Cryo-preserved semen was assessed 24 h after freezing and immediately after thawing. Freezing significantly lowered progressive sperm motility (69.10 ± 0.75 vs 53.81 ± 0.61), intact acrosome (84.42 ± 0.77 vs 75.69 ± 1.10), HOST (60.12 ± 1.19 vs 55.71 ±1.33) and CMPT (31.32 ± 0.70 vs 27.97 ±0.72). Whereas, significantly higher percentages of sperm abnormalities (15.96 ± 0.44 vs 16.92 ± 0.57) were observed after freezing.


Kedia N.,Chhattishgarh Kamdhenu Vishwavidyalaya | Tiwari R.P.,Chhattishgarh Kamdhenu Vishwavidyalaya | Mishra G.K.,Chhattishgarh Kamdhenu Vishwavidyalaya | Awasthi M.K.,Chhattishgarh Kamdhenu Vishwavidyalaya | And 3 more authors.
Indian Journal of Animal Sciences | Year: 2013

The experiment was conducted to study the effects of different levels of liquid nitrogen on various aspects of postthaw seminal characteristics. Semen ejaculates (48) from Tharparkar bulls (6) of 4 cosy ears of age were collected. Post-thaw progressive motility was assessed 24 h freezing. HOST PMT and CPMT of spream decreased on progressive decline in the level of N2, and it was observed that level of N2, should not fall below 1/2 of the level of show while storage of semen of Tharparkar bull.


Sonar B.P.,Chhattishgarh Kamdhenu Vishwavidyalaya | Tiwari R.P.,Chhattishgarh Kamdhenu Vishwavidyalaya | Poyam M.R.,Chhattishgarh Kamdhenu Vishwavidyalaya | Mishra G.K.,Chhattishgarh Kamdhenu Vishwavidyalaya | Sahu S.B.,Chhattishgarh Kamdhenu Vishwavidyalaya
Indian Journal of Animal Sciences | Year: 2014

Incubation test assesses the livability of spermatozoa in female reproductive tract and thereby the longevity of the sperms. Indirectly, it assesses the freezing injuries occurred to sperms during the deep freezing process. The period spent after thawing and before insemination may affect the quality and fertility of post-thaw semen. The frozen semen was thawed at 37-C for 30 sec and taken into a small sugar tubeand incubated in the water-bath at 37-C for 0 h, 15 min, 30min, 45 min and 60 h and per cent post-thaw motility(PTM%), hypo-osmotic swelling test (HOST%) and cervical mucus penetration test (CMPT - mm) were carried out. The PTM declined from 54.33-1.18% at 0 h to 53.00-1.17% -15 min, 47.33-1.08% - 30 min, 43.33-1.05% - 45 min and 35.67-1.27% at 60 min. HOST reduced from 52.53-1.53%at 0 h to 51.07-1.45%- 15 min, 42.67-1.82%- 30 min,33.80-2.36%- 45 min and 23.60-1.10% at 60 min. Similarly, CMPT reduced from 28.00-1.23 mm at 0 h to 8.27-0.56 mm at 60 min. The overall post-thaw motility, HOST and CMPT at 0 h and 15 min were significantly higher than 30 min, 45 min and 60 min in Gir bulls. Post-thaw motility, hypo-osmotic swelling test and cervical mucus penetration test remain unaffected up to 15 min post-thaw incubation while there is significant decline in at 30, 45 and 60 min, which indicated that increasing post-thaw incubation duration affects post-thaw motility, cell membrane permeability and cervical mucus penetration ability of spermatozoa which may affect fertility.

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