Shijiazhuang, China
Shijiazhuang, China

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Xiaoguang Z.,CPLA Bethune International Peace Hospital | Meirong L.,Hebei Medical University | Jingjing Z.,Chest Hospital of Hebei Province | Ruishen Z.,First Engineering Company | And 2 more authors.
Oncology Research | Year: 2017

The long noncoding CPS1 intronic transcript 1 (lncRNA CPS1-IT1) is a recently identified tumor suppressor in the lncRNA family of proteins. Whether this lncRNA plays any functional role in solid tumors remains largely unknown. The present study aimed to investigate the role of lncRNA CPS1-IT1 in human lung cancer. Expression of lncRNA CPS1-IT1 was initially assessed in human lung cancer and in a series of lung cancer cell lines. The effects of CPS1-IT1 overexpression on cell proliferation, migration, and invasion were examined in lung cancer cell lines A549 and 95D. It was found that lncRNA CPS1-IT1 was significantly lower in cancerous tissues than in noncancerous tissues. lncRNA CPS1-IT1 was differentially expressed in lung cancer cell lines and expressed the least in two highly invasive cell lines, A549 and 95D. Overexpression of CPS1-IT1 slowed down cell proliferation by 35.7% in A549 cells and 30.8% in 95D cells on the fifth day. Cell migration was inhibited by 59% in A549 cells and 48% in 95D cells, and cell invasion was suppressed by 60% in both cell lines after overexpression of CPS1-IT1. While cell apoptosis was induced, CPS1-IT1 overexpression promoted the activities of caspase 3 and caspase 9 without affecting that of caspase 8. These observations were suggestive of the tumor-suppressive role of lncRNA CPS1-IT1 in lung cancer. Our data suggest that CPS1-IT1 may be used as a biomarker for early diagnosis and therapeutic targets against lncRNA and may be promising in the treatment of lung cancer. Copyright © 2017 Cognizant, LLC.


Sun J.,Hebei Medical University | Bao J.,Chest Hospital of Hebei Province | Zhang B.,Hebei Medical University | Yuan L.,Peoples Hospital Of Liaocheng | And 5 more authors.
International Journal of COPD | Year: 2017

Background: Proteases may play an important role in the development of chronic obstructive pulmonary disease and emphysema in response to cigarette smoke exposure (CSE). The current study was designed to investigate the expression of matrix metalloproteinase (MMP)-8, MMP-9, MMP-12, tissue inhibitor of MMP (TIMP)-1, and TIMP-4 in rat lung tissues in response to CSE, and assessed the effect of simvastatin in regulating expression of MMPs and TIMPs. Methods: Thirty normal Sprague Dawley (SD) rats were divided into control (n=10), CSE (n=10), and CSE plus simvastatin (n=10) groups. Animals were whole-body exposed to the cigarette smoke in the box for 1 hour each time, twice a day, 5 days a week for 16 weeks. Animals of CSE + simvastatin group were intra-gastrically administered simvastatin at a dose of 5 mg/kg/day followed by CSE. Bronchoalveolar lavage fluid was harvested for inflammatory cell count and lung tissues were stained for morphologic examination. Expression of mRNA and protein level of MMP-8, MMP-9, MMP-12, TIMP-1, and TIMP-4 was assessed by real-time reverse transcription polymerase chain reaction and immunohistochemistry, respectively. Results: CSE resulted in a significant increase of mean linear intercept (MLI: 34.6±2.0 µm) and bronchial wall thickness and diameter (BWT/D, 0.250±0.062) compared to control (MLI: 24.0±1.7 µm, BWT/D: 0.160±0.034, P<0.01). In contrast, mean alveolar number was significantly decreased in the CSE group than that in the control group (13.5±2.0 of CSE vs 21.5±2.0 N/µm2 of control, P>0.01). Simvastatin slightly but not significantly prevented alteration of MLI, BWT/D, and mean alveolar number (MLI: 33.4±1.4 µm; BWT/D: 0.220±0.052; mean alveolar number: 15.5±2.5 N/µm2, P>0.05). Total white blood cell was significantly increased in the bronchoalveolar lavage fluid of smoking group (3.3±2.5×109 cells/L vs 1.1±1.3×109 cells/L of control, P<0.01), and it was significantly reduced by simvastatin (2.3±2.1×109 cells/L, P<0.01). CSE resulted in significantly increased accumulation of neutrophils and macrophages (neutrophils: 14.5%±1.3% of CSE group vs 9.1%±1.5% of control; macrophage: 91%±3% of CSE group vs 87%±2% of control, P<0.05), and simvastatin significantly reduced neutrophils (12.9%±2.0%, P<0.05) in the bronchoalveolar lavage fluid, but had no effect on macrophage (89%±1.6%, P>0.05). In response to CSE, MMP-8, MMP-9, and MMP-12 mRNA were upregulated more than sevenfold, while TIMP-1 and TIMP-4 increased two- to fivefold. Simvastatin significantly blocked upregulation of MMP-8 and -9 (P<0.01), but had no effect on MMP-12, TIMP-1 and TIMP-4 mRNA (P>0.05). In addition, simvastatin significantly blocked cigarette smoke-induced MMP-8 and -9 protein synthesis, while it had no significant effect on TIMP-1 and -4 protein synthesis even in the presence of cigarette smoke. Conclusion: CSE resulted in imbalance of MMPs and TIMPs, and by which mechanism, cigarette smoke may lead to insufficient lung tissue repair. Simvastatin partially blocked airway inflammation and MMP production and, thus, statins may modulate composition of the lung extracellular matrix. © 2017 Sun et al.


Nie Z.,Hebei Medical University | Wu H.,Chest Hospital of Hebei Province | Zhang N.,Hebei Medical University | Zhen X.,Hebei Medical University | And 3 more authors.
Chinese Journal of Andrology | Year: 2015

Objective Explore the most suitable cytoplasmic sperm injection (ICSI) timing for achieving a high fertilization rate, good quality embryo rate and good outcome of pregnancy. Methods According to the oocyte preincubation time (t) defined form the end of the time of egg retrieved to the beginning time for ICSI (T), the patients were divided into three groups including group A (2 h≤t <4 h, n = 174), group B (4 h≤t <6 h, n = 147), and group C (≥6 h, n = 82). A retrospective analysis was carried out about the effcets f the oocytes incubation time on ICSI patients fertilization rate, cleavage rate, high-quality embryo rate and clinical outcome. Results (1) There were no significant differences in fertilization rate (81.5% vs 83.5%), cleavage rate (98.9% vs97.5%), high-quality embryo rate (46.9% vs45.5%), implantation rate (36.7% vs32.8 %), pregnancy rate (55.2% vs551.7%) and abortion rates (12.5% v13.2%) between Group A and group B (P>0.05). (2) Fertilization rate in Group C (88.3%) was significantly higher than that in group A and group B(P<0.05). High quality embryo rate (41.6%), implantation rate (18.7%), pregnancy rate (30.7%) in Group C were all lower obviously than those in group A and group B (P<0.05). Cleavage rate (98.9 percent), abortion rate (12.0%) in group C had no significant differences as compared with that of the other two groups (P>0.05). Conclusion Prolonging preincubation time (≥6 h) may have a high fertility rate, and lower quality embryos, which may tamper clinical outcome of ICSI patients. © 2015, Shanghai Jiaotong University School of Medicine. All rights reserved.


Zhaoyan N.,Hebei Medical University | Haifeng W.,Chest Hospital of Hebei Province | Xiuli Z.,Hebei Medical University | Na Z.,Hebei Medical University | And 3 more authors.
Chinese Journal of Andrology | Year: 2014

Objective To evaluate clinical application of short-period fertilization combined with early rescue intracytoplasmic sperm injection (ICSI) technique in fertilization of severe abnormal sperm. Methods Total 235 patients with severe teratozoospermia (1%≤Normal sperm morphology rate<5%)for in vitro fertilization-embryo transfer (IVF-ET) cycles for egg were randomly divided into two groups. 101 cases of direct implementation of the ICSI as group A, 134 cases for short-period fertilization combined with early rescue ICSI as group B. Among group B, cumulus cells were removed after fertilization 4~5h, second polar body of oocytes was observed, in which 109 cases more than 30% visible bipolar body as B1 group, 25 cases of early judgment no less than 30% of bipolar body performed early remedial ICSI surgery as a group B2.The fertilization rate, cleavage rate, high-quality embryo rate, implantation rate and clinical outcome were comparatively analyzed among different groups. Results Egg maturation rates of three groups were 83.93%, 87.03% and 90.48% respectively, normal fertilization rates were 78.76%, 72.49% and 75.61%, abnormal fertilization rates were 6.11%, 14.88% and 9.76%, cleavage rates were 83.93%, 85.89% and 84.96%, high-quality embryos were 49.45%, 50.49% and 53.23%, implantation rates were 28.10%, 26.67% and 32.00%, clinical pregnancy rates were 43.66%, 43.04% and 47.62%, miscarriage rates were 6.45%, 8.82% and 10.00%. there were no significant difference in egg maturation rate, normal fertilization rate, cleavage rate, high-quality embryo rate, implantation rate, clinical pregnancy, miscarriage rates among three groups different (P >0.05). Abnormal fertilization rate in group B had an increasing trend, but the difference was not statistically significant (P >0.05). Conclusion Short-period fertilization combined with early rescue Intracytoplasmic sperm injection (ICSI) show great value for fertilization of patients with severe abnormal sperm. A good clinical outcome can be obtained if minimizing the ICSI. ©, 2015. Shanghai Jiaotong University School of Medicine. All rights reserved.


Li D.,Chest Hospital of Hebei Province | Yang Y.,Chest Hospital of Hebei Province | Zhu G.,Chest Hospital of Hebei Province | Liu X.,Chest Hospital of Hebei Province | And 3 more authors.
FEBS Letters | Year: 2015

miR-410 acts as an oncogene or tumor suppressor gene in some malignancies. However, its role in NSCLC is still unknown. In this study, we showed that the expression of miR-410 was up-regulated in both human NSCLC tissues and cells. Overexpression of miR-410 promoted cell proliferation, migration, and invasion of NSCLC. In addition, bromodomain-containing protein 7 (BRD7) was a direct target of miR-410. MiR-410-mediated downregulation of BRD7 led to increase Akt phosphorylation. Inhibition of Akt phosphorylation can rescue the effect of miR-410 on NSCLC cell. The expression of BRD7 was downregulated in NSCLC and was inversely expressed with miR-410 in NSCLC. Our data provided new knowledge regarding the role of miR-410 in the lung cancer progression. © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.


Dong Y.-K.,Chest Hospital of Hebei Province | Li A.-Z.,Chest Hospital of Hebei Province | Zheng L.-H.,Chest Hospital of Hebei Province | Chi Y.-P.,Chest Hospital of Hebei Province | And 4 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To explore the value of adenosine deaminase (ADA) activity, interferon-gamma (IFN-γ) and IFN-γ induced protein of 10kD (IP-10) levels in pleural effusion for the diagnosis of tuberculous pleuritis. Methods ADA activity, IFN-γ and IP-10 levels in pleural effusion were determined in sixty-three patients with tuberculous pleuritis and 50 patients with malignant pleural effusion. Results The mean levels of ADA, IFN-γ and IP-10 in the tuberculous pleural effusion were significantly higher than those in malignant pleural effusion (P<0.01). When 45U/L was regarded as cut off value for ADA, the sensitivity, specificity and diagnostic odds ratio in the diagnosis of tuberculous pleurisy were 71.4%, 94.0% and 39.17 respectively. When 138.5pg/ml was regarded as cut off value for IFN-γ in tuberculous pleural effusion, the sensitivity, specificity and diagnostic odds ratio were 93.7%, 82.0% and 67.19 respectively. When 9.21μg/ml was regarded as cut off value for IP-10 in tuberculous pleural effusion, the sensitivity, specificity and diagnostic odds ratio were 85.7%, 90.0% and 54.00 respectively. The combined determination of the three markers for the diagnosis of tuberculous pleurisy had a sensitivity of 95.2%, specificity of 96.0% and diagnostic odds ratio of 72.16. Conclusion The accuracy of diagnosis for tuberculous pleurisy can be improved by combined determination of ADA, IFN-γ and IP-10. © 2015, People’s Military Medical Press. All rights reserved.


PubMed | Chest Hospital of Hebei Province
Type: Journal Article | Journal: Oncotarget | Year: 2016

MiRNAs have been focused for their wide range of biological regulatory functions. Previous studies have suggested that individual miRNAs could influence tumorigenesis through their regulation of specific proto-oncogenes and tumor suppressor genes. This study was implemented to investigate the associations between SNPs in mature microRNAs (miRNAs) and development of lung cancer in a two-stage, case-control study, followed by some functional validations. First, 11 SNPs were analyzed in a case-control study of lung cancer, and the significant results were validated in an additional population. Our results showed that rs3746444 in mir-499 (allele C vs T: OR = 1.33; 95% CI = 1.15-1.54; P = 1.2 10-4) and rs4919510 in mir-608 (allele G vs C: OR = 1.27; 95% CI= 1.13-1.43; P = 5.1 10-5) were significantly associated with increased risk of lung cancer. Rs3746444 in mir-499 was also significantly associated with poor survival of lung cancer (HR, 1.35; 95% CI, 1.15-1.58; P = 0.0002). The expression levels of mir-499 and mir-608 were significantly lower than those of adjacent normal tissues (P < 0.0005), and the carriers of minor alleles have lower expression levels of mir-499 and mir-608 than those of major alleles (P < 0.001). These findings indicated that rs3746444 in mir-499 and rs4919510 in mir-608 might play a substantial role in the susceptibility to lung cancer.

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