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Nie Z.,Hebei Medical University | Wu H.,Chest Hospital of Hebei Province | Zhang N.,Hebei Medical University | Zhen X.,Hebei Medical University | And 3 more authors.
Chinese Journal of Andrology | Year: 2015

Objective Explore the most suitable cytoplasmic sperm injection (ICSI) timing for achieving a high fertilization rate, good quality embryo rate and good outcome of pregnancy. Methods According to the oocyte preincubation time (t) defined form the end of the time of egg retrieved to the beginning time for ICSI (T), the patients were divided into three groups including group A (2 h≤t <4 h, n = 174), group B (4 h≤t <6 h, n = 147), and group C (≥6 h, n = 82). A retrospective analysis was carried out about the effcets f the oocytes incubation time on ICSI patients fertilization rate, cleavage rate, high-quality embryo rate and clinical outcome. Results (1) There were no significant differences in fertilization rate (81.5% vs 83.5%), cleavage rate (98.9% vs97.5%), high-quality embryo rate (46.9% vs45.5%), implantation rate (36.7% vs32.8 %), pregnancy rate (55.2% vs551.7%) and abortion rates (12.5% v13.2%) between Group A and group B (P>0.05). (2) Fertilization rate in Group C (88.3%) was significantly higher than that in group A and group B(P<0.05). High quality embryo rate (41.6%), implantation rate (18.7%), pregnancy rate (30.7%) in Group C were all lower obviously than those in group A and group B (P<0.05). Cleavage rate (98.9 percent), abortion rate (12.0%) in group C had no significant differences as compared with that of the other two groups (P>0.05). Conclusion Prolonging preincubation time (≥6 h) may have a high fertility rate, and lower quality embryos, which may tamper clinical outcome of ICSI patients. © 2015, Shanghai Jiaotong University School of Medicine. All rights reserved.


Zhaoyan N.,Hebei Medical University | Haifeng W.,Chest Hospital of Hebei Province | Xiuli Z.,Hebei Medical University | Na Z.,Hebei Medical University | And 3 more authors.
Chinese Journal of Andrology | Year: 2014

Objective To evaluate clinical application of short-period fertilization combined with early rescue intracytoplasmic sperm injection (ICSI) technique in fertilization of severe abnormal sperm. Methods Total 235 patients with severe teratozoospermia (1%≤Normal sperm morphology rate<5%)for in vitro fertilization-embryo transfer (IVF-ET) cycles for egg were randomly divided into two groups. 101 cases of direct implementation of the ICSI as group A, 134 cases for short-period fertilization combined with early rescue ICSI as group B. Among group B, cumulus cells were removed after fertilization 4~5h, second polar body of oocytes was observed, in which 109 cases more than 30% visible bipolar body as B1 group, 25 cases of early judgment no less than 30% of bipolar body performed early remedial ICSI surgery as a group B2.The fertilization rate, cleavage rate, high-quality embryo rate, implantation rate and clinical outcome were comparatively analyzed among different groups. Results Egg maturation rates of three groups were 83.93%, 87.03% and 90.48% respectively, normal fertilization rates were 78.76%, 72.49% and 75.61%, abnormal fertilization rates were 6.11%, 14.88% and 9.76%, cleavage rates were 83.93%, 85.89% and 84.96%, high-quality embryos were 49.45%, 50.49% and 53.23%, implantation rates were 28.10%, 26.67% and 32.00%, clinical pregnancy rates were 43.66%, 43.04% and 47.62%, miscarriage rates were 6.45%, 8.82% and 10.00%. there were no significant difference in egg maturation rate, normal fertilization rate, cleavage rate, high-quality embryo rate, implantation rate, clinical pregnancy, miscarriage rates among three groups different (P >0.05). Abnormal fertilization rate in group B had an increasing trend, but the difference was not statistically significant (P >0.05). Conclusion Short-period fertilization combined with early rescue Intracytoplasmic sperm injection (ICSI) show great value for fertilization of patients with severe abnormal sperm. A good clinical outcome can be obtained if minimizing the ICSI. ©, 2015. Shanghai Jiaotong University School of Medicine. All rights reserved.


Dong Y.-K.,Chest Hospital of Hebei Province | Li A.-Z.,Chest Hospital of Hebei Province | Chi Y.-P.,Chest Hospital of Hebei Province | Wang Y.-H.,Chest Hospital of Hebei Province | And 3 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To explore the value of adenosine deaminase (ADA) activity, interferon-gamma (IFN-γ) and IFN-γ induced protein of 10kD (IP-10) levels in pleural effusion for the diagnosis of tuberculous pleuritis. Methods ADA activity, IFN-γ and IP-10 levels in pleural effusion were determined in sixty-three patients with tuberculous pleuritis and 50 patients with malignant pleural effusion. Results The mean levels of ADA, IFN-γ and IP-10 in the tuberculous pleural effusion were significantly higher than those in malignant pleural effusion (P<0.01). When 45U/L was regarded as cut off value for ADA, the sensitivity, specificity and diagnostic odds ratio in the diagnosis of tuberculous pleurisy were 71.4%, 94.0% and 39.17 respectively. When 138.5pg/ml was regarded as cut off value for IFN-γ in tuberculous pleural effusion, the sensitivity, specificity and diagnostic odds ratio were 93.7%, 82.0% and 67.19 respectively. When 9.21μg/ml was regarded as cut off value for IP-10 in tuberculous pleural effusion, the sensitivity, specificity and diagnostic odds ratio were 85.7%, 90.0% and 54.00 respectively. The combined determination of the three markers for the diagnosis of tuberculous pleurisy had a sensitivity of 95.2%, specificity of 96.0% and diagnostic odds ratio of 72.16. Conclusion The accuracy of diagnosis for tuberculous pleurisy can be improved by combined determination of ADA, IFN-γ and IP-10. © 2015, People’s Military Medical Press. All rights reserved.


Li D.,Chest Hospital of Hebei Province | Yang Y.,Chest Hospital of Hebei Province | Zhu G.,Chest Hospital of Hebei Province | Liu X.,Chest Hospital of Hebei Province | And 3 more authors.
FEBS Letters | Year: 2015

miR-410 acts as an oncogene or tumor suppressor gene in some malignancies. However, its role in NSCLC is still unknown. In this study, we showed that the expression of miR-410 was up-regulated in both human NSCLC tissues and cells. Overexpression of miR-410 promoted cell proliferation, migration, and invasion of NSCLC. In addition, bromodomain-containing protein 7 (BRD7) was a direct target of miR-410. MiR-410-mediated downregulation of BRD7 led to increase Akt phosphorylation. Inhibition of Akt phosphorylation can rescue the effect of miR-410 on NSCLC cell. The expression of BRD7 was downregulated in NSCLC and was inversely expressed with miR-410 in NSCLC. Our data provided new knowledge regarding the role of miR-410 in the lung cancer progression. © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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