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Chicago Ridge, IL, United States

Pauli G.F.,University of Illinois at Chicago | Pro S.M.,Cherry Instruments | Chadwick L.R.,Cherry Instruments | Burdick T.,Cherry Instruments | And 7 more authors.
Analytical Chemistry | Year: 2015

Countercurrent separation (CCS) utilizes the differential partitioning behavior of analytes between two immiscible liquid phases. We introduce the first platform ("CherryOne") capable of real-time monitoring, metering, and control of the dynamic liquid-liquid CCS process. Automated phase monitoring and volumetrics are made possible with an array of sensors, including the new permittivity-based phase metering apparatus (PMA). Volumetric data for each liquid phase are converted into a dynamic real-time display of stationary phase retention (Sf) and eluent partition coefficients (K), which represent critical parameters of CCS reproducibility. When coupled with the elution-extrusion operational mode (EECCC), automated Sf and K determination empowers untargeted and targeted applications ranging from metabolomic analysis to preparative purifications. © 2015 American Chemical Society. Source

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