Moon J.Y.,Jeju National University |
Nguyen L.T.T.,Jeju National University |
Hyun H.B.,Jeju National University |
Osman A.,Jeju National University |
And 4 more authors.
Journal of Applied Biological Chemistry | Year: 2015
The anticancer activity of a methanolic extract from lemon leaves (MLL) was assessed in MCF-7-SC human breast cancer stem cells. MLL induced apoptosis in MCF-7-SC, as evidenced by increased apoptotic body formation, sub-G1 cell population, annexin V-positive cells, Bax/Bcl-2 ratio, as well as proteolytic activation of caspase-9 and caspase-3, and degradation of poly (ADP-ribose) polymerase (PARP) protein. Concomitantly, MLL induced the formation of acidic vesicular organelles, increased LC3-II accumulation, and reduced the activation of Akt, mTOR, and p70S6K, suggesting that MLL initiates an autophagic progression in MCF-7-SC via the Akt/mTOR pathway. Epithelialmesenchymal transition (EMT), a critical step in the acquisition of the metastatic state, is an attractive target for therapeutic interventions directed against tumor metastasis. At low concentrations, MLL induced anti-metastatic effects on MCF-7-SC by inhibiting the EMT process. Exposure to MLL also led to an increase in the epithelial marker E-cadherin, but decreased protein levels of the mesenchymal markers Snail and Slug. Collectively, this study provides evidence that lemon leaves possess cytotoxicity and antimetastatic properties. Therefore, MLL may prove to be beneficial as a medicinal plant for alternative novel anticancer drugs and nutraceutical products. © The Korean Society for Applied Biological Chemistry 2015.
Son C.S.,Keimyung University |
Kim Y.N.,Keimyung University |
Park K.R.,CheongShim International Academy |
Park H.-J.,Keimyung University
IEICE Transactions on Information and Systems | Year: 2010
A scheme for designing a hierarchical fuzzy classification system with a different number of fuzzy partitions based on statistical characteristics of the data is proposed. To minimize the number of misclassified patterns in intermediate layers, a method of fuzzy partitioning from the defuzzified outputs of previous layers is also presented. The effectiveness of the proposed scheme is demonstrated by comparing the results from five datasets in the UCI Machine Learning Repository. Copyright © 2010 The Institute of Electronics, Information and Communication Engineers.
Kim J.Y.,CheongShim International Academy |
Yoon S.M.,Kookmin University |
Kim Y.-S.,Kookmin University
Journal of the Korean Wood Science and Technology | Year: 2015
Cellulase is the key enzyme in the use of cellulose-based biomaterials. Because of its structure, cellulose is difficult to be degraded by enzymes. In order to utilize cellulose-based biomaterials efficiently, evolutionary wisdom of how to use enzymes accurately and harmoniously in a biological system is needed, such as the cellulose digestive system in animals. In this study, the symbiotic bacteria from snails, Achatina fulica, were identified and their cellulase activity was evaluated. The 16S rRNA sequence analysis of 100 aerobic bacteria showed that they belonged to 9 genus and almost half of the bacteria were Lactococcus spp. Among 100 identified strains, only two Aeromonas sp. strains showed cellulase activity. Aeromonas sp. KMBS020 had both endo-β-glucanase and β-glucosidase activities but Aeromonas sp. KMBS018 had β-glucosidase activity only. None of the 100 bacterial colonies had any cellobiohydrolase activity.
Jeong Y.-J.,Cheongshim International Academy |
Kim C.-W.,Dong - Eui University |
Jeong S.-C.,Dong - Eui University
ICIC Express Letters, Part B: Applications | Year: 2015
The main function of U-City is providing intelligent city management and U-Services through U-City integrated control center. U-Services are important functions to enhance the satisfaction of the user, citizen. Therefore, there is a need to construct the service environment of the U-Service based on analyzing the characteristics and needs of the citizen in the stage of planning. This study presents the user-oriented U-Services implementation strategy of ubiquitous service for successful U-City project by analyzing 370 Busan citizens’ acceptance factors based on UTAUT (Unified Theory of Acceptance and Use of Technology) model. © 2015 ICIC International.
Kim E.J.,Yonsei University |
Pai A.J.,Cheongshim International Academy |
Kang N.-E.,Eulji University |
Kim W.K.,Dankook University |
And 3 more authors.
Nutrition Research and Practice | Year: 2012
The hand hygiene behavior of 400 middle school students (grades 1-3) in Seoul and Gyeonggi-Do was studied to determine how stages of change were affected by food safety education, focusing on hand hygiene and general food safety. Subjects were 51.3% male and 44.3% of study subjects were first graders of middle school. Approximately 40% of subjects were at the stage of action, 42.7% were at the stage of contemplation, and 16.4% were at pre-contemplation. The most important factor that influenced proper hand washing was self efficacy (P < 0.001). Proper hand washing was also correlated significantly with positive belief (P < 0.01) and stages of change (P < 0.01). After food safety education by high-school mentors, middle-school students who were in the stages of pre-contemplation (11.1%) and contemplation (88.9%) showed significant progression toward the action stage (P < 0.001). Proper hand washing (P < 0.01) and food safety knowledge (P < 0.05) were also significantly increased after educational intervention. © 2012 The Korean Nutrition Society and the Korean Society of Community Nutrition.
PubMed | Pai Chai University and Cheongshim International Academy
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2016
Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene