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PubMed | Hospital Universitario San Cecilio, University of Michigan, Chenzhou No1 Peoples Hospital, Unidade Of Imunologia Clinica Centro Hospitalar Do Porto and 7 more.
Type: Journal Article | Journal: Annals of the rheumatic diseases | Year: 2016

Systemic lupus erythematosus (SLE) is a clinically heterogeneous disease with limited reliable diagnostic biomarkers. We investigated whether gene methylation could meet sensitivity and specificity criteria for a robust biomarker.IFI44L promoter methylation was examined using DNA samples from a discovery set including 377 patients with SLE, 358 healthy controls (HCs) and 353 patients with rheumatoid arthritis (RA). Two independent sets including 1144 patients with SLE, 1350 HCs, 429 patients with RA and 199 patients with primary Sjgrens syndrome (pSS) were used for validation.Significant hypomethylation of two CpG sites within IFI44L promoter, Site1 (Chr1: 79085222) and Site2 (Chr1: 79085250; cg06872964), was identified in patients with SLE compared with HCs, patients with RA and patients with pSS. In a comparison between patients with SLE and HCs included in the first validation cohort, Site1 methylation had a sensitivity of 93.6% and a specificity of 96.8% at a cut-off methylation level of 75.5% and Site2 methylation had a sensitivity of 94.1% and a specificity of 98.2% at a cut-off methylation level of 25.5%. The IFI44L promoter methylation marker was also validated in an European-derived cohort. In addition, the methylation levels of Site1 and Site2 within IFI44L promoter were significantly lower in patients with SLE with renal damage than those without renal damage. Patients with SLE showed significantly increased methylation levels of Site1 and Site2 during remission compared with active stage.The methylation level of IFI44L promoter can distinguish patients with SLE from healthy persons and other autoimmune diseases, and is a highly sensitive and specific diagnostic marker for SLE.


PubMed | BGI Shenzhen, Chenzhou No1 Peoples Hospital and Changsha Maternal and Child Health Hospital
Type: Journal Article | Journal: Oncotarget | Year: 2016

Phelan-McDermid syndrome is a neurodevelopmental disorder caused by the terminal deletion of chromosome 22 (22q13) followed by the loss of function of the SHANK3 gene. Various terminal deletions of chromosome 22q13 are associated with Phelan-McDermid with a spectrum of phenotypic severity. Here, we have done a clinical molecular study of a Chinese proband with Phelan-McDermid syndrome. Both the proband and her younger brother are associated with this syndrome while their parents are phenotypically normal. We used a karyotype in order to detect the genotype of the proband and her younger brother. We have also used whole genome low-coverage paired-end next generation sequencing to determine whether the parent is the carrier of translocation with terminal 22q13 deletions. We found that both proband and her younger brother are comprises of a novel deletion of 22q13.31q13.33, harboring genes were associated with several clinical phenotype such as severity of speech delay, neonatal hypotonia, delayed in age of walking, male genital anomalies, dysplastic toenails, large and fleshy hands, macrocephaly, short stature, facial asymmetry, and atypical reflexes. Probands and her younger brother inherited this translocation from their mother whereas their father is genotypically normal. In conclusion, our present study expands the deletion spectrum and report a novel deletion associated with Phelan-McDermid syndrome.


Deng F.-Z.,The Fourth Peoples Hospital of Chenzhou | Kuang G.-P.,Chenzhou No1 Peoples Hospital
International Eye Science | Year: 2015

AIM: To observe the situations of different surgical methods on dry eyes in patients with pterygium excision combined transplantation. METHODS: Seventy-eight cases ofpterygium patients (81 eyes) were randomly divided into three groups. Group A underwent pterygium excision combined large autologous conjunctival flap transplantation; group B underwent pterygium excision combined with small conjunctival flap; group C underwent pterygium excision combined with small conjunctival flap with autologous limbal stem cell. Repair of postoperative corneal epithelium, 1, 3mo preoperation and postoperation tear film break up time (BUT) and questionnaire of ocular surface disease index (OSDI) were observed among three groups, which caused the situation of dry eyes by pterygium and pterygium excision were evaluated. RESULTS: BUT: Group A was shorter than that in groups B and C at 15d postoperation (P<0.05); at 1mo postoperation, group A was no statistical difference with groups B and C (P>0.05). Postoperative dry eye ratio of group A was higher than that in groups B and C at 1mo postoperation (P<0.05). There was no statistical difference between group B and group C (P>0.05), but group C showed lower postoperative dry eye ratio. Corneal epithelium recover time of group A was longer than that in groups B and C (P<0.05). There was no statistical difference between group B and group C (P>0.05), but group C showed a tendency to be shorter recover time. CONCLUSION: Pterygium excision combined with small conjunctival flap and autologous limbal stem cell shows guickly corneal epithelium recover and low dry eye ratio and deserve to recommended. Copyright 2015 by the IJO Press.


Kuang Z.-H.,Chenzhou No1 Peoples Hospital | Li H.-P.,Chenzhou No1 Peoples Hospital
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2015

This paper analyzed clinical data of 6 patients with central pontine myelinolysis (CPM). Among them 4 cases were male, 2 cases were female, with age 24 to 55 years old and average age 36 years old. Serious somatic diseases occured in all 6 cases. The main clinical manifestations included disturbance of consciousness, pseudobulbar paralysis and flaccid paralysis. Cranial MRI showed all of the patients appeared symmetrical lesions in central pontine. After intravenous drip of glucocorticoids, 5 patients were improved. Copyright © 2015 by the Editorial Board of Chinese Journal of Contemporary Neurology and Neurosurgery.


Li B.,Cancer Center | Liu W.,Cancer Center | Liu W.,Chenzhou No1 Peoples Hospital | Wang L.,Cancer Center | And 7 more authors.
Annals of Surgical Oncology | Year: 2010

Background. CpG island methylator phenotype (CIMP), characterized by simultaneous methylation of multiple tumor suppressor genes (TSGs), has been reported to be associated with biological malignancy in many cancers. Whether CIMP is potentially predictive of clinical outcome in hepatocellular carcinoma (HCC) remains unknown. Methods. We investigated the methylation status of ten TSGs and CIMP in 115 samples of HCC and 48 samples of corresponding nonneoplastic liver tissues using a methylationspecific polymerase chain reaction. Results. The methylation frequencies of the ten genes examined in HCC were 40.0% for p14ARF, 60.9% for p15INK4b, 70.4% for p16 INK4a, 34.8% for p73, 70.4% for GSTP1, 64.3% for MGMT, 13.0% for hMLH1, 59.1% for RARb, 82.6% for SOCS-1, and 80.9% for OPCML. CIMP+ (with six or more methylated genes) was detected in 68 (59.1%) of 115 HCCs and none of 48 nonneoplastic liver tissues. On stratified univariate analysis, patients with tumor-node-metastasis (TNM) stage I HCC with CIMP+ had significantly shorter overall survival (OS) (P = 0.002) and recurrence-free survival (RFS) (P = 0.042) than those with CIMP-. Furthermore, multivariate analysis revealed CIMP+ as an independent prognostic factor for both OS [hazard ratio (HR), 12.266; P = 0.015] and RFS (HR, 2.275; P = 0.032) in TNM stage I patients. Conclusions. CIMP+ may specifically define a subgroup of patients with unfavorable outcome in TNM stage I HCC. Examination of CIMP status may be useful for stratifying prognosis of patients with early-stage HCC and identifying patients who are at higher risk for recurrence. © Society of Surgical Oncology 2010.


Li X.,Foshan University | Xu Q.,Thomas Jefferson University | Wu Y.,Central South University | Li J.,Hunan provincial Tumor Hospital | And 3 more authors.
Carcinogenesis | Year: 2014

Cancer-associated fibroblasts (CAFs) have been described to play critical roles in initiation, progression and metastasis of various cancers. However, the involvement of CAFs in oral cancer (OC) has not been well addressed. In this study, we demonstrate that CAFs, when cocultured with OC cells (OCCs), produce high levels of chemokine (C-C motif) ligand 2 (CCL2) and, subsequently, enhance endogenous reactive oxygen species production in cells. Oxidative stress stimulates expression of cell cycle progression proteins in OCCs, leading to promotion of OCC proliferation, migration, invasion and, OC tumor growth. On the other hand, oxidative stress triggered the activation of nuclear factor-kappaB (NF-κB) and STAT3 in CAFs, resulting in accelerating CCL2 expression. In this way, CAFs-OCCs coculture creates a favorable cytokine-rich microenvironment, beneficial for both CAFs and OCCs. In addition, upregulation of CCL2 expression has been observed in oral squamous cell carcinoma tumors and patient plasma. We also showed that inhibition of CCL2 reduced OC tumor burden in mice. Therefore, our data suggested that CCL2 represents a potential therapeutic target for treatment of OC. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.


Gao P.,Shanghai Normal University | Wang Z.,Chenzhou No1 Peoples Hospital | Yang L.,Shanghai Normal University | Ma T.,Shanghai Normal University | And 3 more authors.
Electrochimica Acta | Year: 2014

Aminophenylboronic acid moieties were covalently grafted onto mercaptobenzoic acid moieties, and glucose oxidase was then immobilized through boronic acid-diol specific recognition to form a pH-sensitive electrosensor switching between pH 5.8 and pH 8.0 base solution. Using potassium ferricyanide as electroactive probe, the response was intensified in acidic condition while hindered in alkaline condition. The sharp and stable contrast in current was performed alternately upon the change of pH like a "pH switch". In the presence of glucose, the response to glucose was further amplified catalytically by glucose oxidase on "ON" state, while electron transfer was inhibited on "OFF" state. Furthermore, when our sensor was on "ON" state, it showed a good linearity ranging from 0 to 30 μmol L-1 of glucose, with a detection limit of 348 nmol L-1 (S/B = 3) and a dynamic range extending to 50 μmol L-1. Glucose-responsive, pH-switchable and catalytically-amplified, our biosensor provided a new method for the detection of glucose in the form of pH switch in human serum sample, and was promising to more complicated environment. © 2014 Elsevier Ltd. All rights reserved.


Diao C.-Y.,Central South University | Guo H.-B.,Central South University | Ouyang Y.-R.,Central South University | Zhang H.-C.,Central South University | And 4 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014

Objective: The aim of this study was to screen for possible biomarkers of metastatic osteosarcoma (OS) using a DNA microarray. Methods: We downloaded the gene expression profile GSE49003 from Gene Expression Omnibus database, which included 6 gene chips from metastatic and 6 from non-metastatic OS patients. The R package was used to screen and identify differentially expressed genes (DEGs) between metastatic and non-metastatic OS patients. Then we compared the expression of DEGs in the two groups and sub-grouped into up-regulated and down-regulated, followed by functional enrichment analysis using the DAVID system. Subsequently, we constructed an miRNA-DEG regulatory network with the help of WebGestalt software. Results: A total of 323 DEGs, including 134 up-regulated and 189 down-regulated, were screened out. The up-regulated DEGs were enriched in 14 subcategories and most significantly in cytoskeleton organization, while the down-regulated DEGs were prevalent in 13 subcategories, especially wound healing. In addition, we identified two important miRNAs (miR-202 and miR-9) pivotal for OS metastasis, and their relevant genes, CALD1 and STX1A. Conclusions: MiR-202 and miR-9 are potential key factors affecting the metastasis of OS and CALD1 and STX1A may be possible targets beneficial for the treatment of metastatic OS. However, further experimental studies are needed to confirm our results.


PubMed | Chenzhou No1 Peoples Hospital, University of South China and Foshan University
Type: | Journal: Experimental cell research | Year: 2017

Cancer associated fibroblasts (CAFs) are known to be involved in initiation, progression and metastasis of various cancers. However, the molecular mechanism of how CAFs affects the biological function of oral cancer (OC) has not been fully-addressed. In this study, we demonstrated that miR-124 was downregulated in oral CAFs and oral cancer cells (OCCs) when compared with matched normal fibroblasts (NFs). Hypermethylation in the promoter region of miR-124 genes was accounted for its downregulation. Interestingly, CAFs but not NFs exerted promotion effect on OCCs cell proliferation, migration and tumor growth in CAFs/NFs-OCCs co-culture. Furthermore, we identified Chemokine (C-C motif) ligand 2 (CCL2) and Interleukin 8 (IL-8) as two direct targets of miR-124. Over-expression of miR-124 in CAFs-OCCs co-culture abrogated CAFs-promoted OCCs cell growth and migration, and this inhibitory effect can be rescued by addition of CCL2 and IL-8. Finally, we showed that restoration of miR-124 expression by lentiviral infection or formulated miR-124 injection inhibited oral tumor growth in vivo suggesting miR-124 rescue could be a potential rationale for therapeutic applications in oral cancer in the future.


PubMed | Chenzhou No1 Peoples Hospital
Type: Journal Article | Journal: Journal of applied clinical medical physics | Year: 2016

The construction of a conventional prostate needle (seeds) implant template restricts needles tilting or incline insertion when it is necessary to approach a seminal vesicle or to avoid the obstruction of symphysis pubis. To overcome the disadvantages of conventional templates, we developed a special template for guiding needles incline insertion and fixation for prostate needle implant. Phantom needles implantation was performed. Two acrylic boards, each 7.5 cm in width by 7.5 cm in length and 0.5 cm thickness, were drilled with a set of domed holes and cones with embedded template ball inside this combination to provide firm grip and fixation in prostate needle implantation. The specially designed domed-cones combination acrylic board provides a needle of up to 60 rotation flexibility application. Some areas that could not be covered in a conventional parallel needle holes template could now be covered by using this new template. The covering index of prostate radiation dosage is up to 84.5%. The specially designed domed-cones acrylic board combination provides not only a reliable means of needle fixation and rotational function, but also a superior dose distribution in the anterior portion of the prostate and good coverage of a seminal vesicle. This special template is a feasible design for prostate needles or seeds implant brachytherapy.

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