Chenzhou No1 Peoples Hospital
Chenzhou No1 Peoples Hospital
Cao C.,Chenzhou No1 Peoples Hospital |
Gu J.,The First Affiliated Hospital Of Xian Jiaotong University |
Zhang J.,The First Affiliated Hospital Of Xian Jiaotong University
Frontiers of Medicine | Year: 2017
Sensitive and useful biomarkers for the diagnosis and prognosis of infectious diseases have been widely developed. An example of these biomarkers is triggering receptor expressed on myeloid cell-1 (TREM-1), which is a cell surface receptor expressed on monocytes/macrophages and neutrophils. TREM-1 amplifies inflammation by activating the TREM-1/DAP12 pathway. This pathway is triggered by the interaction of TREM-1 with ligands or stimulation by bacterial lipopolysaccharide. Consequently, pro-inflammatory cytokines and chemokines are secreted. Soluble TREM-1 (sTREM-1) is a special form of TREM-1 that can be directly tested in human body fluids and well-known biomarker for infectious diseases. sTREM-1 level can be potentially used for the early diagnosis and prognosis prediction of some infectious diseases, including infectious pleural effusion, lung infections, sepsis, bacterial meningitis, viral infections (e.g., Crimean Congo hemorrhagic fever and dengue fever), fungal infections (e.g., Aspergillus infection), and burn-related infections. sTREM-1 is a more sensitive and specific biomarker than traditional indices, such as C-reactive protein and procalcitonin levels, for these infectious diseases. Therefore, sTREM-1 is a feasible biomarker for the targeted therapy and rapid and early diagnosis of infectious diseases. © 2017 Higher Education Press and Springer-Verlag Berlin Heidelberg
Chen B.,Southern Medical University |
Chen B.,Chenzhou No1 Peoples Hospital |
Bao Y.,Southern Medical University |
Bao Y.,Yue Bei Peoples Hospital Guangdong Province |
And 7 more authors.
Biomedicine and Pharmacotherapy | Year: 2015
Background: Uncontrol cell growth and proliferation is acknowledged to responsible for cancer-related deaths by disorganizing the balance of growth promotion and growth limitation. Aberrant expression of microRNA play essential roles in cancer development, leads to cell proliferation, growth and survival, and promotes the development of various human tumors, including osteosarcoma. Elucidating the molecular mechanism of this abnormality in osteosarcoma carcinogenesis may improve diagnostic and therapeutic strategies for this malignancy. Methods: The expression of miR-664 in osteosarcoma cell lines and osteosarcoma tissues was examined using real-time PCR. The effects of miR-664 on osteosarcoma cell proliferation were evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, colony formation and Anchorage-independent growth ability assay. The effect of miR-664 on FOXO4 was determine by luciferase assays and western blot assay. Results: The expression of miR-664 was markedly upregulated in osteosarcoma cell lines and tissues, and upregulation of miR-664 enhanced, whereas downregulation of miR-664 inhibited the proliferation of osteosarcoma cells in vivo. Furthermore, using bioinformatics and biological approaches, we showed that miR-664 directly targeted and suppressed the expression of tumor suppressors FOXO4. Conclusions: Our findings suggest that miR-664 functions as an oncogene miRNA and has an important role in promoting human osteosarcoma cell proliferation by suppressing FOXO4 expression. These data suggests that miR-664 may represent a novel therapeutic target of microRNA-mediated suppression of cell proliferation in osteosarcoma. © 2015.
Xu S.,Central South University |
Xu S.,Chenzhou No1 Peoples Hospital |
Peng J.,Central South University |
Li Y.,Central South University |
And 4 more authors.
Journal of Ethnopharmacology | Year: 2012
Ethnopharmacological relevance: Wu-Chu-Yu is a well-known herbal drug used for hypertension. Rutaecarpine and evodiamine are main bioactive components of the medicine. Materials and methods: A sensitive and specific HPLC method was developed to analyze rutaecarpine (Rut) and evodiamine (Evo) in rat whole blood. The pharmacokinetics of Rut and Evo after oral administration of Wu-Chu-Yu extracts with different purities to rats was compared to evaluate the effect of purity of Wu-Chu-Yu extracts on the absorption of Rut and Evo. Male Sprague-Dawley rats were given Wu-Chu-Yu extracts with different purities (high, medium and low) approximately the same doses of equivalent to Rut (40 mg/kg) and Evo (31 mg/kg). The contents of Rut and Evo were 45 and 35%, 28 and 21%, 9 and 7% in high, medium and low purity extracts, respectively. At different time points (0.25, 0.5, 0.75, 1, 1.5, 2, 2.5, 3 and 4 h) after administration, the concentrations of Rut and Evo in rat whole blood were determined by HPLC, and main pharmacokinetic parameters were calculated. Results: The results indicated that the absorption of Rut and Evo in Wu-Chu-Yu extracts was improved when compared with the pure Rut and Evo and there were significant differences among different groups. Conclusions: The bioavailability of Rut and Evo was increased along with the increasing of purity (16%-80%) in Wu-Chu-Yu extracts. © 2011 Elsevier Ireland Ltd.
Deng F.-Z.,The Fourth Peoples Hospital of Chenzhou |
Kuang G.-P.,Chenzhou No1 Peoples Hospital
International Eye Science | Year: 2015
AIM: To observe the situations of different surgical methods on dry eyes in patients with pterygium excision combined transplantation. METHODS: Seventy-eight cases ofpterygium patients (81 eyes) were randomly divided into three groups. Group A underwent pterygium excision combined large autologous conjunctival flap transplantation; group B underwent pterygium excision combined with small conjunctival flap; group C underwent pterygium excision combined with small conjunctival flap with autologous limbal stem cell. Repair of postoperative corneal epithelium, 1, 3mo preoperation and postoperation tear film break up time (BUT) and questionnaire of ocular surface disease index (OSDI) were observed among three groups, which caused the situation of dry eyes by pterygium and pterygium excision were evaluated. RESULTS: BUT: Group A was shorter than that in groups B and C at 15d postoperation (P<0.05); at 1mo postoperation, group A was no statistical difference with groups B and C (P>0.05). Postoperative dry eye ratio of group A was higher than that in groups B and C at 1mo postoperation (P<0.05). There was no statistical difference between group B and group C (P>0.05), but group C showed lower postoperative dry eye ratio. Corneal epithelium recover time of group A was longer than that in groups B and C (P<0.05). There was no statistical difference between group B and group C (P>0.05), but group C showed a tendency to be shorter recover time. CONCLUSION: Pterygium excision combined with small conjunctival flap and autologous limbal stem cell shows guickly corneal epithelium recover and low dry eye ratio and deserve to recommended. Copyright 2015 by the IJO Press.
Kuang Z.-H.,Chenzhou No1 Peoples Hospital |
Li H.-P.,Chenzhou No1 Peoples Hospital
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2015
This paper analyzed clinical data of 6 patients with central pontine myelinolysis (CPM). Among them 4 cases were male, 2 cases were female, with age 24 to 55 years old and average age 36 years old. Serious somatic diseases occured in all 6 cases. The main clinical manifestations included disturbance of consciousness, pseudobulbar paralysis and flaccid paralysis. Cranial MRI showed all of the patients appeared symmetrical lesions in central pontine. After intravenous drip of glucocorticoids, 5 patients were improved. Copyright © 2015 by the Editorial Board of Chinese Journal of Contemporary Neurology and Neurosurgery.
Li B.,Cancer Center |
Liu W.,Cancer Center |
Liu W.,Chenzhou No1 Peoples Hospital |
Wang L.,Cancer Center |
And 7 more authors.
Annals of Surgical Oncology | Year: 2010
Background. CpG island methylator phenotype (CIMP), characterized by simultaneous methylation of multiple tumor suppressor genes (TSGs), has been reported to be associated with biological malignancy in many cancers. Whether CIMP is potentially predictive of clinical outcome in hepatocellular carcinoma (HCC) remains unknown. Methods. We investigated the methylation status of ten TSGs and CIMP in 115 samples of HCC and 48 samples of corresponding nonneoplastic liver tissues using a methylationspecific polymerase chain reaction. Results. The methylation frequencies of the ten genes examined in HCC were 40.0% for p14ARF, 60.9% for p15INK4b, 70.4% for p16 INK4a, 34.8% for p73, 70.4% for GSTP1, 64.3% for MGMT, 13.0% for hMLH1, 59.1% for RARb, 82.6% for SOCS-1, and 80.9% for OPCML. CIMP+ (with six or more methylated genes) was detected in 68 (59.1%) of 115 HCCs and none of 48 nonneoplastic liver tissues. On stratified univariate analysis, patients with tumor-node-metastasis (TNM) stage I HCC with CIMP+ had significantly shorter overall survival (OS) (P = 0.002) and recurrence-free survival (RFS) (P = 0.042) than those with CIMP-. Furthermore, multivariate analysis revealed CIMP+ as an independent prognostic factor for both OS [hazard ratio (HR), 12.266; P = 0.015] and RFS (HR, 2.275; P = 0.032) in TNM stage I patients. Conclusions. CIMP+ may specifically define a subgroup of patients with unfavorable outcome in TNM stage I HCC. Examination of CIMP status may be useful for stratifying prognosis of patients with early-stage HCC and identifying patients who are at higher risk for recurrence. © Society of Surgical Oncology 2010.
Li X.,Foshan University |
Xu Q.,Thomas Jefferson University |
Wu Y.,Central South University |
Li J.,Hunan provincial Tumor Hospital |
And 3 more authors.
Carcinogenesis | Year: 2014
Cancer-associated fibroblasts (CAFs) have been described to play critical roles in initiation, progression and metastasis of various cancers. However, the involvement of CAFs in oral cancer (OC) has not been well addressed. In this study, we demonstrate that CAFs, when cocultured with OC cells (OCCs), produce high levels of chemokine (C-C motif) ligand 2 (CCL2) and, subsequently, enhance endogenous reactive oxygen species production in cells. Oxidative stress stimulates expression of cell cycle progression proteins in OCCs, leading to promotion of OCC proliferation, migration, invasion and, OC tumor growth. On the other hand, oxidative stress triggered the activation of nuclear factor-kappaB (NF-κB) and STAT3 in CAFs, resulting in accelerating CCL2 expression. In this way, CAFs-OCCs coculture creates a favorable cytokine-rich microenvironment, beneficial for both CAFs and OCCs. In addition, upregulation of CCL2 expression has been observed in oral squamous cell carcinoma tumors and patient plasma. We also showed that inhibition of CCL2 reduced OC tumor burden in mice. Therefore, our data suggested that CCL2 represents a potential therapeutic target for treatment of OC. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: email@example.com.
Gao P.,Shanghai Normal University |
Wang Z.,Chenzhou No1 Peoples Hospital |
Yang L.,Shanghai Normal University |
Ma T.,Shanghai Normal University |
And 3 more authors.
Electrochimica Acta | Year: 2014
Aminophenylboronic acid moieties were covalently grafted onto mercaptobenzoic acid moieties, and glucose oxidase was then immobilized through boronic acid-diol specific recognition to form a pH-sensitive electrosensor switching between pH 5.8 and pH 8.0 base solution. Using potassium ferricyanide as electroactive probe, the response was intensified in acidic condition while hindered in alkaline condition. The sharp and stable contrast in current was performed alternately upon the change of pH like a "pH switch". In the presence of glucose, the response to glucose was further amplified catalytically by glucose oxidase on "ON" state, while electron transfer was inhibited on "OFF" state. Furthermore, when our sensor was on "ON" state, it showed a good linearity ranging from 0 to 30 μmol L-1 of glucose, with a detection limit of 348 nmol L-1 (S/B = 3) and a dynamic range extending to 50 μmol L-1. Glucose-responsive, pH-switchable and catalytically-amplified, our biosensor provided a new method for the detection of glucose in the form of pH switch in human serum sample, and was promising to more complicated environment. © 2014 Elsevier Ltd. All rights reserved.
Diao C.-Y.,Central South University |
Guo H.-B.,Central South University |
Ouyang Y.-R.,Central South University |
Zhang H.-C.,Central South University |
And 4 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014
Objective: The aim of this study was to screen for possible biomarkers of metastatic osteosarcoma (OS) using a DNA microarray. Methods: We downloaded the gene expression profile GSE49003 from Gene Expression Omnibus database, which included 6 gene chips from metastatic and 6 from non-metastatic OS patients. The R package was used to screen and identify differentially expressed genes (DEGs) between metastatic and non-metastatic OS patients. Then we compared the expression of DEGs in the two groups and sub-grouped into up-regulated and down-regulated, followed by functional enrichment analysis using the DAVID system. Subsequently, we constructed an miRNA-DEG regulatory network with the help of WebGestalt software. Results: A total of 323 DEGs, including 134 up-regulated and 189 down-regulated, were screened out. The up-regulated DEGs were enriched in 14 subcategories and most significantly in cytoskeleton organization, while the down-regulated DEGs were prevalent in 13 subcategories, especially wound healing. In addition, we identified two important miRNAs (miR-202 and miR-9) pivotal for OS metastasis, and their relevant genes, CALD1 and STX1A. Conclusions: MiR-202 and miR-9 are potential key factors affecting the metastasis of OS and CALD1 and STX1A may be possible targets beneficial for the treatment of metastatic OS. However, further experimental studies are needed to confirm our results.
PubMed | Chenzhou No1 Peoples Hospital, University of South China and Foshan University
Type: | Journal: Experimental cell research | Year: 2017
Cancer associated fibroblasts (CAFs) are known to be involved in initiation, progression and metastasis of various cancers. However, the molecular mechanism of how CAFs affects the biological function of oral cancer (OC) has not been fully-addressed. In this study, we demonstrated that miR-124 was downregulated in oral CAFs and oral cancer cells (OCCs) when compared with matched normal fibroblasts (NFs). Hypermethylation in the promoter region of miR-124 genes was accounted for its downregulation. Interestingly, CAFs but not NFs exerted promotion effect on OCCs cell proliferation, migration and tumor growth in CAFs/NFs-OCCs co-culture. Furthermore, we identified Chemokine (C-C motif) ligand 2 (CCL2) and Interleukin 8 (IL-8) as two direct targets of miR-124. Over-expression of miR-124 in CAFs-OCCs co-culture abrogated CAFs-promoted OCCs cell growth and migration, and this inhibitory effect can be rescued by addition of CCL2 and IL-8. Finally, we showed that restoration of miR-124 expression by lentiviral infection or formulated miR-124 injection inhibited oral tumor growth in vivo suggesting miR-124 rescue could be a potential rationale for therapeutic applications in oral cancer in the future.