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Zhao W.,Shandong Cancer Hospital and Institute | Yang L.,Shandong Cancer Hospital and Institute | Wang L.,Qingdao Chengyang Peoples Hospital | Zuo W.,Shandong Cancer Hospital and Institute | And 8 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

Aims: This study is to retrospectively analyze the clinical and pathological data of six cases of primary nasal clear cell carcinoma in our hospital since 1992 and to review literatures on the clinical manifestations, pathological features, immunohistochemistry, diagnosis and treatment of the disease. Methods: The pathological archives that were diagnosed as salivary gland nasal tumors in Shandong Cancer Hospital during 1992-2013 were reviewed. Careful review of sections was performed by two experienced pathologists. The samples were labeled using EnVision method. Immunostaining was performed using 3, 3’-diaminobenzidine reagent followed by counterstaining with hematoxylin. The immunohistochemical results were classified according to positive cells: no positive staining cells (-); positive cells <30% (+); positive cells between 30% and 50% (++); and positive cells >50% (+++). Results: Among the 6 cases of primary nasal clear cell carcinoma in our hospital since 1992, 4 cases were diagnosed as clear cell carcinoma of nasal cavity after exclusion of other nasal cavity tumors with clear cells, and 2 cases were directly diagnosed as clear cell carcinoma of nasal cavity. Hyalinizing clear cell carcinoma of salivary gland (HCCC) tissues were mainly composed of polygonal epithelioid tumor cells arranged into the shapes of beehives, and separated by fibrous tissues containing rich thin-wall capillaries. The cytoplasm of HCCC cells was rich and translucent with some cells having multiple vacuoles. Reticular fiber staining showed that the tumor cells were arranged in shapes of beehives and separated by rich reticular fibers. HCCC tumor reacted differently on S-100 protein, glial fibrillary acidic protein, actin and vimentin. The ultrastructure of HCCC cells showed characteristics of ducts but no myoepithelial differentiation. Conclusions: This study demonstrates that correct diagnosis, timely surgical resection and postoperative radiotherapy are effective in treating nasal clear cell carcinoma. © 2014, E-Century Publishing Corporation. All rights reserved.

Cao Y.,Qingdao Chengyang Peoples Hospital | Zhang X.,Qingdao Chengyang Peoples Hospital
Applied Microbiology and Biotechnology | Year: 2013

Hydroxy fatty acids (HFAs) are very important chemicals for versatile applications in biodegradable polymer materials and cosmetic and pharmaceutical industries. They are difficult to be synthesized via chemical routes due to the inertness of the fatty acyl chain. In contrast, these fatty acids make up a major class of natural products widespread among bacteria, yeasts, and fungi. A number of microorganisms capable of producing HFAs from fatty acids or vegetable oils have been reported. Therefore, HFAs could be produced by biotechnological strategies, especially by microbial conversion processes. Microorganisms could oxidize fatty acids either at the terminal carbon or inside the acyl chain to produce various HFAs, including α-HFAs, β-HFAs, mid-position HFAs, ω-HFAs, di-HFAs, and tri-HFAs. The enzymes and their encoded genes responsible for the hydroxylation of the carbon chain have been identified and characterized during the past few years. The involved microbes and catalytic mechanisms for the production of different types of HFAs are systematically demonstrated in this review. It provides a better view of HFA biosynthesis and lays the foundation for further industrial production. © 2013 Springer-Verlag Berlin Heidelberg.

Qiu F.-X.,Qingdao Chengyang Peoples Hospital | Song L.,Peoples Care | Su J.,Qingdao Chengyang Peoples Hospital | Liu X.-M.,Qingdao University
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Currently the pathogenesis of skin lesions in chronic renal failure patients has not yet been fully elucidated. Stem cell factor is a multifunctional cytokine, which may play a crucial role in the process of complications of chronic kidney disease. Changes related to stem cell factor levels in the peripheral blood of maintenance hemodialysis patients have been rarely reported. OBJECTIVE: To investigate the changes of serum stem cell factor levels in maintenance hemodialysis patients and their relationships with uremic pruritus. METHODS: Serum levels of stem cell factors in 86 maintenance hemodialysis patients were detected by enzyme-linked immunosorbent assay and compared with statistical methods. Visual analogue scale was to assess the severity of pruritus in patients with uremia. Then based on the scores, patients were divided into four groups: group A, 0-2 scores (n=23); group B, 3-5 scores (n=21); group C, 6-8 scores (n=24); and group D, > 8 scores (n=18). The differences in serum stem cell factor levels among the four groups were determined by Fisher's least significant difference test. The correlation between stem cell factor levels and the severity of pruritus, blood hemoglobin and intact parathyroid hormone were analyzed by Pearson correlation analysis. RESULTS AND CONCLUSION: Gender, age, body mass index and blood pressure were all not statistically different among different groups (P > 0. 05). With the progression of uremic pruritus, serum levels of stem cell factor in the peripheral blood increased (P < 0. 05), blood hemoglobin levels gradually decreased (P < 0. 05), and levels of intact parathyroid hormone increased (P < 0. 05). Serum levels of stem cell factors in the peripheral blood was negatively related to blood hemoglobin (r=-0. 60, P < 0. 01), but positively correlated to intact parathyroid hormone (r=0. 70, P < 0. 01). These findings indicate that serum levels of stem cell factors in the peripheral blood may play an important role in the occurrence and development of uremic pruritus.

Li H.,Mianyang Central Hospital | Li H.,Southern Medical University | Wang J.,Mianyang Central Hospital | Ouyang B.,Peoples Hospital of Jiangsu Province | And 8 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Background: This study aims to investigate the attitude and knowledge of Chinese physicians towards obstructive sleep apnea syndrome (OSAS). Methods: Seven hospitals in China including 580 doctors were organized to participate in this study. In this present investigation, a self-reported questionnaire that aims to assess the at­titude and knowledge of physicians towards OSAS was used. Results: A total of 563 questionnaires were returned. Among the 560 respondents who completed the questionnaires, 532 (95%) respondents thought snoring might be a disease and 507 (80.5%) respondents knew what OSAS is. Respondents in the relevant professional group (RP group) were more familiar with OSAS than those in the non-related professional group (NRP group) (P=0.027), and both groups strongly agreed on these two statements: “OSAS needs to be treated aggressively or given attention” and “knowledge of OSAS needs to be enhanced” (P > 0.05). Mean OSAS knowledge score of physicians was 44.87 ± 20.64. The RP group had a higher score than the NRP group, P=0.009. Based on location, Southern hospitals had a higher score than Northern hospitals (P=0.026), while Western hospitals had a higher score than Eastern hospitals (P=0.006). Tertiary level hospitals had a higher score compared with lower-level hospitals (P=0.000). Respondents had higher scores when a sleep center was present in the hospital (P=0.012). Respondents with higher professional titles had higher scores (P=0.000). Treatments from highest to lowest scores were as follows: weight loss, quit smoking and alcohol, continuous positive airway pressure (CPAP), avoid fatigue, surgery and drugs. Conclusions: Doctors recognized that snoring might be a disease and they recommended aggressive treatment or give attention for OSAS. However, their knowledge of OSAS was poor. They were willing to further their knowledge of OSAS. © 2016, E-Century Publishing Corporation. All rights reserved.

Sun A.-M.,Qingdao Chengyang Peoples Hospital | Li D.-T.,Shandong Cancer Hospital | Mu D.-B.,Shandong Cancer Hospital | Sun C.,Qingdao Chengyang Peoples Hospital | Li B.-B.,Shandong Cancer Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2013

OBJECTIVE: To elucidate the expression of p16, Ki-67 in Esophageal Squamous Cell Carcinoma(ESCC) and its relationship with clinicopathologic characteristics and prognosis of patients of ESCC. METHODS: Expressions of p16 and Ki-67 were determined by SP imunohistochemical technique in 63 consecutive patients with ESCC. The prognosis of ESCC was evaluated by Kaplan-Meier survival analysis, and the relationship between expression of p16 and Ki-67 and clinicopathological indices were analyzed by statistics. RESULTS: The loss rate of p16 and the positive rate of Ki-67 were 58.73%(37/63), 65.08%(41/63), respectively. The loss expression of the p16 and the high expression of Ki-67 was positive correlation(P=0.001), and it had a positive correlation with clinical stage(P<0.001) and lymphnode metastasis(P were 0.010 and 0.008) too. It was no relation with tumor size (P were 0.396 and 0.098) and histological grading(P were 0.514 and 0.389). Kaplan-Meier survival analysis curve displayed that the p16 expression rate and Ki-67 increasing rate and survival time were positively correlated (P<0.01). Multivariate Cox regression analysis showed that p16 (P=0.038) and Ki-67 (P=0.044) expression, lymph node metastasis (P=0.021), clinical staging (P=0.031), postoperative treatment (P=0.004) were the independent risk factors of prognosis of patients. CONCLUSIONS: In esophageal squamous cell carcinoma the loss of p16 expression and Ki-67 expression increase with the clinical staging, prognosis and lymph node metastasis of the disease. p16 and Ki-67 detection may be used in esophageal squamous cell carcinoma in staging and as important prognostic molecular markers.

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