Qiao F.,Baoding University |
Du J.,Chenguang Biotech
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013
A simple and selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) method coupled with high performance liquid chromatography (HPLC) ultraviolet detection was developed for rapid screening of clenbuterol hydrochloride (CH) in chicken samples. The new molecularly imprinted microspheres (MIM) were synthesized by using butylamine and chloroaniline as dummy template with aqueous suspension polymerization and revealed good affinity to CH in aqueous solution. The application of the obtained MIM as sorbent of matrix solid-phase dispersion (MSPD) improved the selectivity of extraction procedure and avoided the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity of CH was obtained in a range of 0.059-18.30μgmL-1 with the correlation coefficient (R) of 0.9996. The recoveries of CH at three spiked levels were ranged from 92.0 to 99.1% with the relative standard deviation less than 4.0% (n=3). The presented MI-MSPD-HPLC method combined the superiority of MIM and MSPD, and therefore could be potentially applied for the determination of CH in complicated biological samples. © 2013 Elsevier B.V.
China Agricultural University and Chenguang Biotech | Date: 2012-03-14
The invention discloses a method for extracting plant-derived natural products with polarity or medium polarity, it comprises shattering the plant-derived materials containing plant-derived natural products with polarity or medium polarity, followed by adding water, raising the pressure to 5-50 MPa at the temperature of 40-90C and circularly feeding high-pressure carbon dioxide to conduct treatment for 1-60 min. The method according to the present invention can be used not only for the extraction of polarity natural products such as anthocyanins, but also for the extraction of other natural products with polarity or medium polarity.
Wu N.-Y.,Shangqiu Normal University |
Gao W.,Chenguang Biotech |
He X.-L.,South China Normal University |
Chang Z.,Shangqiu Normal University |
Xu M.-T.,Shangqiu Normal University
Biosensors and Bioelectronics | Year: 2013
A direct electrochemical DNA biosensor based on zero current potentiometry was fabricated by immobilization of ssDNA onto gold nanoparticles (AuNPs) coated pencil graphite electrode (PGE). One ssDNA/AuNPs/PGE was connected in series between clips of working and counter electrodes of a potentiostat, and then immersed into the solution together with a reference electrode, establishing a novel DNA biosensor for specific DNA detection. The variation of zero current potential difference (ΔEzcp) before and after hybridization of the self-assembled probe DNA with the target DNA was used as a signal to characterize and quantify the target DNA sequence. The whole DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of ferricyanide as an electrochemical redox indicator. Under the optimized conditions, ΔEzcp was linear with the concentrations of the complementary target DNA in the range from 10nM to 1μM, with a detection limit of 6.9nM. The DNA biosensor showed a good reproducibility and selectivity. Prepared DNA biosensor is facile and sensitive, and it eliminates the need of using exogenous reagents to monitor the oligonucleotides hybridization. © 2012 Elsevier B.V..
Chenguang Biotech | Date: 2011-12-31
A process for extracting lycopene, comprising the following steps: pressing and dehydrating tomato pomace which is the by-products of tomato processing production, then drying it to control the water content in the range from 10% to 20%; crushing the dried tomato pomace, and separating tomato skins and tomato seeds by air blast process, granulating the separated tomato skins and extracting them, then purifying by removing impurity from the extracted lycopene with active carbon. The process uses the by-products of tomato production as raw material, thus increasing the utilization ratio of tomatoes; the way of separating the seeds and skins after dehydrating and drying can save water and reduce the discharge of pollutant; the addition of antioxidant in the process of drying avoids the impact of high temperature on lycopene; extracting after granulating the tomato skins significantly increases the extracting efficiency; treating the extracting solution with active carbon effectively can remove the pesticide residues, impurities, odor etc., and thus increase the quality of lycopene.
Chenguang Biotech | Date: 2013-12-30
The present invention relates to a method for producing silymarin. The method comprises: pressing silybum marianum seeds to obtain silymarin powder, and soaking the silymarin powder by using alkaline water; extracting, by using acetone, the silymarin powder soaked by using alkaline water, and performing filtering and concentration on the extracted liquid to obtain a concentrated solution; and performing extraction on the concentrated solution by using a non-polar solvent, performing separation to obtain a non-polar solvent layer and an acetone layer, and concentrating and drying the acetone layer to obtain silymarin. The method can greatly improve the extraction efficiency and the yield, shorten the extraction time, needs simple processes and low cost and thoroughly remove residual oil, so that the product has a low impurity, high product quality and a high purity; and the method is applicable to industrial production and has a great application prospect and economic value.
Chenguang Biotech | Date: 2011-12-31
Disclosed a process for preparing a xanthophyll crystal, comprising: dissolving the plant extract containing a xanthophyll ester in n-hexane, then filtering the mixture; adding acetone to the filtrate, filtering and collecting a filter cake; mixing the filter cake with soybean oil and ethanol uniformly; saponifying the mixed solution with alkaline aqueous solution; then adding an acidic solution thereto until the mixed solution becomes acidic, concentrating under reduced pressure to obtain a pasty substance; adding n-hexane to the pasty saponified product, standing still and then conducting a solid-liquid separation; washing the resulting solid substance with deionized water; adding a mixed solvent to the washed solid substance, dissolving it with stirring; and then adding n-hexane thereto and standing still to recrystallize. According to the application, organic solvents are used to treat the plant extract and remove non-xanthophyll ester compounds in order to improve the efficiency of the saponification reaction; the saponified solution is concentrated under acidic condition at reduced pressure, then extracted with an organic solvent for saving water; purifying a xanthophyll crystal with a mixed solvent in order to significantly increase the purity of a xanthophyll crystal and proportion of trans-xanthophyll.
Chenguang Biotech | Date: 2012-12-19
Anticorrosive paints; Colorants; Dyestuffs; Food dyes; Ink cartridges for printers; Ink jet cartridges; Natural resins; Paints; Printing ink; Printing toner.
Chenguang Biotech | Date: 2012-12-19
Cosmetics; Cosmetics for animals; Detergents for household use; Incense; Perfume; Polishing preparations; Polishing, scouring and abrasive preparations; Soaps for personal use; Tooth paste; Washing powder.
Chenguang Biotech | Date: 2013-06-07
Chili oil for use as a seasoning or condiment; Flavorings; Flavorings for beverages, other than essential oils; Flavourings for cakes other than essential oils; Food flavorings; Molasses; Propolis for human consumption; Seasonings; Spices; Turmeric.