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Lan J.,University of Sichuan | Wan X.-L.,University of Sichuan | Deng L.,University of Sichuan | Xue J.-X.,University of Sichuan | And 7 more authors.
Radiation Research | Year: 2013

Ablative hypofractionated radiotherapy (HFRT) significantly improves the overall survival of inoperable non-small cell lung cancer (NSCLC) patients compared with conventional radiation therapy. However, the radiobiological mechanisms of ablative HFRT remain largely unknown. The purpose of this study was to investigate the dynamic changes of tumor vessels and perfusion during and after ablative hypofractionated radiotherapy. Lewis lung carcinoma-bearing mice were treated with sham (control) and ablative hypofractionated radiotherapy of 12 Gy in 1 fraction (12 Gy/1F) and 36 Gy in 3 fractions (36 Gy/3F). Tumor microvessel density (MVD), morphology and function were examined at different times after irradiation. The results showed that, compared to the controls the MVD and hypoxia in ablative HFRT groups decreased, which were accompanied by an increase in the number of pericytes and their coverage of vessels. Functional tests revealed that tumor hypoxia and perfusion were improved, especially in the 36 Gy/3F group. Our results revealed that ablative hypofractionated radiotherapy not only repressed MVD and hypoxia, but also increased the vascular perfusion and the number of pericyte-covered vessels, suggesting that ablative HFRT normalized the tumor vasculature. © 2013 by Radiation Research Society. Source

Sun C.-J.,Chengdu Third Peoples Hospital | Li C.,Sichuan Cancer Hospital | Lv H.-B.,Civil Aviation Medical Center | Zhao C.,Chengdu Third Peoples Hospital | And 7 more authors.
Journal of Radiation Research | Year: 2014

The aim of this study was to evaluate the oxygen partial pressure of the rabbit model of the VX2 tumor using a 64-slice perfusion CT and to compare the results with that obtained using the oxygen microelectrode method. Perfusion CT was performed for 45 successfully constructed rabbit models of a VX2 brain tumor. The perfusion values of the brain tumor region of interest, the blood volume (BV), the time to peak (TTP) and the peak enhancement intensity (PEI) were measured. The results were compared with the partial pressure of oxygen (PO2) of that region of interest obtained using the oxygen microelectrode method. The perfusion values of the brain tumor region of interest in 45 successfully constructed rabbit models of a VX2 brain tumor ranged from 1.3-127.0 (average, 21.1 ± 26.7 ml/min/ml); BV ranged from 1.2-53.5 ml/100g (average, 22.2 ± 13.7 ml/100g); PEI ranged from 8.7-124.6 HU (average, 43.5 ± 28.7 HU); and TTP ranged from 8.2-62.3 s (average, 38.8 ± 14.8 s). The PO2 in the corresponding region ranged from 0.14-47 mmHg (average, 16 ± 14.8 mmHg). The perfusion CT positively correlated with the tumor PO2, which can be used for evaluating the tumor hypoxia in clinical practice. © 2013 The Author. Source

Xue L.,University of Sichuan | Zha L.,Chengdu Womens and Childrens Central Hospital | Chen Q.,Chengdu Third Peoples Hospital | Liang Y.-J.,Chengdu Third Peoples Hospital | And 5 more authors.
The Scientific World Journal | Year: 2013

The objective of this study was to compare the clinical results and complications of proximal femoral nail antirotation (PFNA) on treatment of intertrochanteric fractures in 120 elderly Chinese patients using Randomized Controlled Trials (RCTs). Totaly 120 cases enrolled were randomly assigned to a lateral decubitus position group and supine position group. The hospital stay, operating time, intraoperative blood loss, length of incision, X-ray fluoroscopy time, and out-of-bed activity time in the lateral decubitus position group were significantly lower than those in the supine position group. There was not statistical significance on union time and Harris values in the two position groups. Moreover, only complications of superficial wound infection were observed in the lateral decubitus position group, but two complications of deep venous thrombosis and wound deep infection were found in the supine position group. The present findings suggested that PFNA applied in elderly patients with intertrochanteric fracture can get satisfactory effects, and the treatment of intertrochanteric fractures using lateral decubitus position showed a satisfactory clinical outcome and a lower radiological complication rate. © 2013 Li Xue et al. Source

Rui J.,Chengdu Third Peoples Hospital | Long D.,University of Sichuan | Wang W.,Chengdu Third Peoples Hospital | Chou T.,Chengdu Third Peoples Hospital | And 6 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2010

Objective To investigate the effect of valproic acid (VPA) on MICA gene expression in human lung carcinoma cell lines: A549, SP-CA-I, NCIH446. Methods Different concentration of VPA were applied to treat A549, SP-CA-1, NCIH446 cells respectively and the untreated cells as the control. The effect of the VPA on the cells were observed. Reverse Transcriptase PCR and Immune-Fluorescence staining were used to detect the changes of mRNA, protein level of MICA gene in the three cell lines treated with 1. 50 mmol/L and 3. 00 mmol/L VPA for 4 days, under the concentration of VPA from 0. 75 to 12. 0 mmol/L. The viability of lung cancer cells after NK effect in the VPA (+) and VPA (-) treatment were evaluated with LDH method. Results The inhibition effects were observed when the VPA concentration was 6. 0 mmol/L above compared to control group, VPA induced the increasing expression of MICA and in turn enhanced the NK killing effect on the cells in a concentrationdepended pattern. Conclusion VPA induced MICA expression, NK killing effect in the VPA(+) treatment lung cancer cells was significantly stronger than that of VPA(-) condition, which implied a new mechanism of anticancer method and could be a new approach to cancer therapy. Source

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