Chen X.,Chengdu Second Peoples Hospital |
Chen Q.-M.,Chengdu Second Peoples Hospital
Journal of Clinical Dermatology | Year: 2017
A case of cutaneous bronchogenic cyst on the neck is reported. A forty-four years old woman presented with repeated ulceration and discharge on her right neck for over 8 years. Physical examinations showed that an ulcer about 0.5cm×0.5 cm in size with explicit boundaries and secretion on the right neck skin. Histopathology showed cyst lined by columnar cells in the middle and deep dermis. A diagnosis of bronchogenic cyst was made. After excision, patient was followed up for half a year without recurrence.
PubMed | Shanxi Medical University, Peoples Hospital of Sichuan Province, Central South University and Chengdu Second Peoples Hospital
Type: Journal Article | Journal: Clinical and experimental hypertension (New York, N.Y. : 1993) | Year: 2016
Our aim is to investigate the prevalence, awareness, treatment, and control status of hypertension and explore the associated factors among Sichuan Tibetan population.A cross-sectional investigation was conducted in the Sichuan Tibetan region from to September 2013 to March to 2014. Three thousand two hundred and forty persons were included in the study through a multi-stage stratified clustering sampling. Participants received questionnaires and physical examination. Blood pressure (BP) was recorded three times after 5 min of rest with the mean taken as the final BP. Hypertension was defined according the 2010 Chinese guidelines for the management of hypertension.Prevalence of hypertension was 45.7%. The proportion of different stages (1-3) of hypertension was 31.7%, 9.2%, and 7.6% for males and 26.3%, 9.5%, and 6.0% for females. The rate of awareness, treatment, and control of hypertension were 4.9%, 21.3%, and 3.0% for males and 7.5%, 24.5%, and 3.1% for females. Multiple-factor analysis found that age, overweight or obesity (odds ratio(OR)=1.16), drinking alcohol (OR = 1.29, 95% confidence interval (CI):1.07-1.54), increased waist circumference (OR = 1.81, 95%CI: 1.39-2.36), family history of hypertension (OR = 1.51, 95%CI; 1.28-1.78), higher salt intakes (OR = 1.62, 95%CI: 1.24-2.11), long duration of sleep (OR = 1.05, 95%CI: 1.01-1.09), rural area (OR = 1.99, 95%CI: 1.64-2.41), and drinking coffee at times (OR = 0.71, 95%CI:0.57-0.87) were related to hypertension.Prevalence of hypertension in Sichuan Tibetan was significantly higher than the national level with low rates of awareness, treatment, and control of hypertension. Intervention measurements are needed to change some unhealthy lifestyles, behaviors, and habits in this region.
Qi Q.,Shandong University |
Wang W.,Chengdu Second Peoples Hospital |
Li T.,Shandong University |
Zhang Y.,Shandong University |
Li Y.,Shandong University
Respirology | Year: 2015
Background and objective Bronchiectasis is a chronic respiratory disease with diverse causes that may differ in clinical features and thus treatment options. However, few large-scale studies on the aetiology of bronchiectasis are currently available. This study aims to determine aetiology and clinical features of bronchiectasis in a Chinese Han population. Methods This prospective study enrolled adult patients diagnosed with bronchiectasis as confirmed by high-resolution computed tomography at five general hospitals in Shandong from January 2010 to August 2014. Causes of bronchiectasis were sought by analysis of clinical history and auxiliary examinations (including serum immunoglobulin determination, saccharin test, Aspergillus skin prick test, autoantibody detection and electronic bronchoscopy). Results A total of 476 adult patients with bronchiectasis were included, and all patients were of Chinese Han ethnicity. Idiopathic (66.0%) was the most common cause, followed by post-tuberculosis (16.0%). Other uncommon causes included post-infective (3.8%), immunodeficiency (3.8%), allergic bronchopulmonary aspergillosis (4.0%), rheumatic diseases (4.4%) and primary ciliary dyskinesia (0.9%). Patients with post-tuberculosis bronchiectasis had a higher frequency of upper lobe involvement (P < 0.05). Cylindrical bronchiectasis was the most common type of all causes, with varicose bronchiectasis occurring more frequently in post-tuberculosis bronchiectasis and allergic bronchopulmonary aspergillosis (P < 0.05). However, patients with different causes did not differ in lung function and sputum isolation rate of Pseudomonas aeruginosa (P > 0.05). Conclusions In a Chinese Han population in Shandong, idiopathic bronchiectasis is the most common form of bronchiectasis followed by post-tuberculosis bronchiectasis. Patients with different causes differ in distribution and pattern of bronchiectasis on computed tomography. © 2015 Asian Pacific Society of Respirology.
Yang M.,Chengdu Second Peoples Hospital |
Du Y.,Chengdu Second Peoples Hospital |
Xu Z.,Chengdu Second Peoples Hospital |
Jiang Y.,Chongqing Medical University
Inflammation | Year: 2016
Upregulation of WISP1 has been demonstrated in lung remodeling. Moreover, it has been recently found that some signaling components of WNT pathway can activate GSK3β signaling to mediate remodeling of airway smooth muscle (ASM) in asthma. Therefore, we hypothesized that WISP1, a signaling molecule downstream of the WNT signaling pathway, is involved in PI3K/GSK3β signaling to mediate ASM remodeling in asthma. Our results showed that WISP1 depletion partly suppressed OVA-induced ASM hypertrophy in vivo. In vitro, WISP1 could induce hBSMC hypertrophy and proliferation, accompanied by upregulation of levels of PI3K, p-Akt, p-GSK3β, and its own expression. TGF-β treatment could increase expression of PI3K, p-Akt, p-GSK3β, and WISP1. SH-5 treatment could partly suppress TGF-β-induced hypertrophy and proliferation of hBSMC, and depress expression of p-GSK3β and WISP1. In conclusion, WISP1 may be a potential inducer of ASM proliferation and hypertrophy in asthma. The pro-remodeling effect of WISP1 is likely due to be involved in PI3K-GSK3β-dependent noncanonical TGF-β signaling. © 2015, Springer Science+Business Media New York.
Chen T.,University of Sichuan |
Chen T.,Chengdu second peoples hospital |
Guo Z.-P.,University of Sichuan |
Wang W.-J.,Chengdu second peoples hospital |
And 3 more authors.
Experimental Dermatology | Year: 2014
High-mobility group box-1 (HMGB1) has been implicated as a pro-inflammatory cytokine in the pathogenesis of various inflammatory and autoimmune diseases. However, information about HMGB1 in Henoch-Schönlein purpura (HSP) is still unclear. Herein, we investigated the role of HMGB1 in patients with HSP and the pro-inflammatory effects of HMGB1 on human dermal microvascular endothelial cell line (HMEC-1). Serum HMGB1 levels in patients with HSP together with patients with allergic vasculitis (AV) and urticarial vasculitis (UV) were detected by enzyme-linked immunosorbent assay (ELISA). HMEC-1 cells were treated with HMGB1 at concentrations ranging from 4 ng/ml to 100 ng/ml. Serum HMGB1 levels were significantly increased in patients with HSP, AV and UV, when compared with those in control group. Moreover, abundant cytoplasmic expression of HMGB1 was observed in endothelial cells in lesional skin of HSP patients. Using membrane cytokine antibody array, we indicate that HMGB1 markedly induced TNF-α and IL-6 release in cultured supernatant. Furthermore, by real-time quantitative PCR and ELISA, the effects of HMGB1 on these cytokines production in HMEC-1 cells were established. Finally, Western blot data revealed that HMGB1 can induce phosphorylation of inhibitor of κB-α (IκBα) and the nuclear translocation of nuclear factor-κB (NF-κB) p65 in HMEC-1 cells. In conclusion, this study provides first observations on the association of HMGB1 with HSP. We suggest that HMGB1 may be an important mediator of endothelial inflammation through the induction of TNF-α and IL-6 production and may play a crucial role in the pathogenesis of HSP. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Yang Y.,Chongqing Medical University |
Shi H.,Chengdu Second Peoples Hospital |
Li X.,Chongqing Medical University |
Yi Y.,Chongqing Medical University
Oncology Reports | Year: 2011
Maspin is a member of the serpin (serine protease inhibitor) family of protease inhibitors known to have tumor suppressor activity in diverse human cancers. However, maspin gene function and the molecular aspects in gastric carcinoma remain largely unclear. To investigate the effects of maspin on invasion of gastric carcinoma SGC7901 cell line and the underlying molecular mechanism involved in this process, we cloned short hairpin oligoes (shRNA) targeting maspin into plasmid pGenesil-1.1 eukaryotic expression vector and then transfected the recombinant plasmid pGenesil-maspin into gastric carcinoma SGC7901 cells using Lipofectamine 2000. After the maspin expression was successfully knocked down, the number of cells invading through Matrigel was obviously increased (P<0.05) in the Transwell chamber assay. By detection of reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively, we found that mRNA and protein of uPA, VEGF-C were increased significantly, and the protein level of MMP7 was also increased (P<0.05). These results suggested that maspin gene could inhibit invasion of gastric cancinoma SGC7901 cells and this inhibition maybe result from the interaction between maspin and uPA, MMP7, or VEGF-C.
Xuan L.,Chengdu Second Peoples Hospital |
Baohua Y.,Chengdu Second Peoples Hospital |
Lan B.,Chengdu Second Peoples Hospital
Indian Journal of Dermatology | Year: 2014
A 26-year-old Chinese male consulted with the team regarding his alopecia areata and vitiligo for which previous treatment was ineffective. The patient, a homosexual man, denied having a history of drug abuse and of blood transfusion. No member of his family had vitiligo or alopecia. Laboratory studies revealed that the serum for anti-human immunodeficiency virus (HIV) antibody was positive. The patient's CD4 lymphocyte count and CD4/CD8 ratio were both strikingly low (20 cells/mL and 0.04), but no other complaints or opportunistic infections were reported. One month after antiretroviral therapy, the patient's alopecia areata dramatically improved, but no evident improvement in his vitiligo was found. This case is a very rare case of alopecia areata and vitiligo associated with HIV infection that might be attributed to the generation and maintenance of self-reactive CD8+ T-cells due to chronic immune activation with progressive immune exhaustion in HIV infection.
PubMed | University of Electronic Science and Technology of China and Chengdu Second Peoples Hospital
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016
Dysfunction of glomerular endothelial cells (GECs) induces a variety of symptoms, including proteinuria, inflammation, vascular diseases, fibrosis and thrombosis. Thrombomodulin (TM) acts as a vasoprotective molecule on the surface of the vascular endothelial cells to maintain the homeostasis of the endothelial microenvironment by suppressing cellular proliferation, adhesion and inflammatory responses. Liver Xreceptor (LXR), a nuclear receptor (NR) and a bile acidactivated transcription factor, regulates metabolism and cholesterol transport, vascular tension and inflammation. Previous studies indicated that TM expression is upregulated by various NRs; however, it is unclear whether pharmacological modulation of LXR may affect TM expression and GEC function. The current study revealed that LXR activation by its agonist, T0901317, upregulates the expression and activity of TM. This effect was mediated specifically through LXR, and not through LXR. Additionally, T0901317 treatment inhibited nuclear factorB (NFB) signaling and the secretion of high glucoseinduced proinflammatory mediators, including tumor necrosis factor and interleukin1 in GECs. Coimmunoprecipitation experiments determined that treatment with T0901317 enhances the interaction between LXR and the transcriptional coactivator, p300, in GEC extracts. The present findings suggest that NFB may be a negative regulator of TM expression, and its removal may contribute to TM gene expression, particularly when in competition with the T0901317enhanced formation of the LXR/p300 complex. Therefore, LXR may be a novel molecular target for manipulating TM in GECs, which may advance the treatment of endothelial cellassociated diseases.
PubMed | Chongqing Medical University and Chengdu Second Peoples Hospital
Type: Journal Article | Journal: Inflammation | Year: 2016
Upregulation of WISP1 has been demonstrated in lung remodeling. Moreover, it has been recently found that some signaling components of WNT pathway can activate GSK3 signaling to mediate remodeling of airway smooth muscle (ASM) in asthma. Therefore, we hypothesized that WISP1, a signaling molecule downstream of the WNT signaling pathway, is involved in PI3K/GSK3 signaling to mediate ASM remodeling in asthma. Our results showed that WISP1 depletion partly suppressed OVA-induced ASM hypertrophy in vivo. In vitro, WISP1 could induce hBSMC hypertrophy and proliferation, accompanied by upregulation of levels of PI3K, p-Akt, p-GSK3, and its own expression. TGF- treatment could increase expression of PI3K, p-Akt, p-GSK3, and WISP1. SH-5 treatment could partly suppress TGF--induced hypertrophy and proliferation of hBSMC, and depress expression of p-GSK3 and WISP1. In conclusion, WISP1 may be a potential inducer of ASM proliferation and hypertrophy in asthma. The pro-remodeling effect of WISP1 is likely due to be involved in PI3K-GSK3-dependent noncanonical TGF- signaling.
PubMed | Guangzhou University, Hospital of Chengdu Office of Peoples Government of Tibetan Autonomous Region and Chengdu Second Peoples Hospital
Type: Journal Article | Journal: World journal of surgery | Year: 2016
Transanal tubes (TTs) have been used to prevent and reduce anastomotic leakage after rectal cancer surgery. The aim of this review was to investigate the efficacy and safety of the TT.A systematic literature search was performed to identify randomized controlled trials and controlled clinical trials assessing the clinical efficacy and safety of TTs in rectal cancer surgery.Seven trials with 1609 participants were included. The TT group had a lower anastomotic leakage rate than the non-transanal tube group [RR 0.38; 95% confidence interval (CI) 0.25-0.58; P<0.0001], as well as a lower reoperation rate (RR 0.31; 95% CI 0.19-0.53; P<0.0001) and a shorter hospital stay (mean=-2.59days; 95% CI -3.69 to -1.49; P<0.0001). There were no significant differences in mortality between the two groups.TT use in rectal cancer surgery is likely to be an effective and safe method of preventing and reducing anastomotic leakage and is associated with a decreased risk of reoperation and faster recovery.