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Sun X.,Sichuan Agricultural University | Lei T.,Chengdu Rongsheng Pharmaceuticals Co. | Du J.-B.,Sichuan Agricultural University | Yang W.-Y.,Sichuan Agricultural University
International Journal of Agriculture and Biology

Plastid terminal oxidase (PTOX) is a plastoquinol oxidase, which plays several important roles in plants. Previous studies suggested that PTOX is encoded by a single gene with only one copy in higher plants. Here we report the identification of two possible paralogous PTOX genes on different chromosomes of soybean, both of which are highly homologous to known PTOX genes in other species. These two paralogs have quite different introns and nearly the same exons and were predicted to encode membrane protein with chloroplast transit peptide. The deduced PTOX protein encoded by both paralogs were proposed to be functional, since the existence of highly conserved amino acid sites necessary for a typical PTOX, including six iron-binding sites and Exon 8 domain. Moreover, soybean PTOX also exhibit clear sequence similarity to alternative oxidase (AOX). Organ-specific expression analysis showed high transcript levels of soybean PTOX in stems, leaves and flowers, while the levels in pods and roots were relatively low. In addition, a light-inducible character was also suggested for soybean PTOX in the present study. © 2015 Friends Science Publishers. Source

Zhang W.,Peking Union Medical College | Ke L.,Peking Union Medical College | Changqing L.,Peking Union Medical College | Zhang Y.,Chengdu Rongsheng Pharmaceuticals Co. | Li W.,Peking Union Medical College
Journal of Translational Medicine

Background: To ensure the safety of plasma derivatives, screening for human parvovirus B19V genomic DNA in donated plasma using a pooling strategy is performed in some countries. We investigated the prevalence of B19V DNA and anti-B19V antibodies in Chinese plasma pools, plasma derivatives and plasma donations to evaluate the risk posed by B19V.Methods: Using a Q-PCR assay developed in-house, we tested for B19V genomic DNA in 142 plasma pools collected between January 2009 and June 2011 from two Chinese blood products manufacturers. Plasma derivatives collected between 1993-1995 (10 batches of albumin, 155 batches of intravenous immunoglobulin, IVIG) and 2009-2011 (50 batches of albumin, 54 batches of IVIG, 35 batches of factor VIII, 7 batches of fibrinogen, and 17 batches of prothrombin complex concentrate, PCC) were also tested for B19V contamination. In addition, B19V genome prevalence in minipools(including 90 individual donations) of 49680 individual plasma samples collected between August 2011 and March 2012 by a single Chinese manufacturer was investigated. IgM/IgG was also investigated in plasma pools/derivatives and in minipools with B19V-DNA titers above 1x104 and 1x106 geq/mL using B19 ELISA IgM/IgG assay(Virion-Serion, Würzburg, Germany), respectively.Results: B19V-DNA was detected in 54.2% of plasma pools from two Chinese blood product manufacturers; among recently produced blood products, B19V was detected in 21/54 IVIG samples, 19/35 factor VIII samples, 6/7 fibrinogen samples, and 12/17 PCC samples, but not in albumin samples. The levels of B19V-DNA in these samples varied from 102-107 geq/mL. In samples with >104 geq/mL genome DNA, B19V-specific IgG was also found in all corresponding plasma pools and IVIG, whereas none was detected in the majority of other plasma derivatives. Screening of plasma donations indicated that most minipools were contaminated with B19V-DNA (102-108 geq/mL) and one donation had 1.09 × 1010 geq/mL B19V genomic DNA along with a non-classical IgG/IgM profile.Conclusions: Despite the implementation of some inactivation/removal methods designed to prevent viral contamination, B19V DNA was detectable in Chinese plasma pools and plasma derivatives. Thus, the introduction of B19V screening and discard donation with high viramic concentration for Chinese plasma donors would be desirable. © 2012 Zhang et al.; licensee BioMed Central Ltd. Source

Sun X.-G.,PUMC Hospital | Liu X.-Y.,PUMC Hospital | Zhu R.,Chengdu Rongsheng Pharmaceuticals Co. | Fan G.-S.,PUMC Hospital | And 2 more authors.
Acta Academiae Medicinae Sinicae

Objective To explore the effectiveness of intravenous immunoglobulin (IVIG) in treating patients with unexplained recurrent spontaneous abortion ( URSA) and the effect of IVIG on the level of soluble human leucocyte antigen G (sHLA-G). Methods This prospective trial conducted at PUMC Hospital between 2004 and 2008 included 60 women with URSA. The patients were allocated into IVIG group (30 cases) and control group (30 cases). IVIG was intravenously used before conception at a dose of 0. 2 g/kg; once pregnancy was confirmed, IVIG was continued every 4 weeks till the 20 gestational week. Traditional Chinese medicine or/and progesterone were used in control group. The outcome of pregnancy was evaluated by live birth rate and effective rate (defined as the embryo living 4 week longer than previous pregnancy). Serum samples were collected randomly before pregnancy and in the 6-8 gestational week from IVIG group (15 samples) , control group (15 samples) , and healthy women (20 samples). The levels of sHLA-G, interferonγ (IFNγ), interleukin-2 (IL-2) , and interleukin-10 (IL-10) were determined by enzyme-linked immunosorbant assay (ELIZA). Results The pregnancy rate was 93. 3% in IVIG group. The live birth rate and effective rate were 85. 7% (24/28) and 92. 9% (26/28) in IVIG group, which were significantly higher than those in control group [56. 7% (17/30) (P = 0.021) and 63.3% (19/30) (P = 0.011)]. Emesis occurred in one woman (3. 3%) in IVIG group had during IVIG infusion but was relieved by lowering the speed of infusion. The mean sHLA-G level was (61.37±35.57) U/ml in control group and (62.70±37.24) U/ml in IVIG group (P>0.05); both of them were significantly lower than that of healthy women (88.49±25.37) U/ml (P<0.05). After pregnancy was achieved, the levels of sHLA-G and IL-10 were (34.19±14.21) U/ml and (11.71±2.75) pg/ml, respectively in the IVIG group, which were significantly higher than those in control group [(23.71±12.83) U/ml and (8.71±3.01) pg/ml, respectively] (P = 0.008). Conclusions Low-dose IVIG before and after pregnancy is a safe and effective in treating URSA. IVIG improves the development of fetus by up-regulating sHLA-G and IL-10 levels. Source

Wang J.-Y.,University of Sichuan | Wang J.-Y.,Chengdu Rongsheng Pharmaceuticals Co. | Yu H.-R.,University of Sichuan | Xie R.,University of Sichuan | And 4 more authors.
AIChE Journal

A novel type of core-shell capsules with ultrathin alginate/protamine/silica (APSi) hybrid membranes are successfully fabricated through a coextrusion minifluidic approach and a biosilicification method for immobilization of laccase. The ultrathin membranes were beneficial to the mass transfer across the capsule membranes, and the silica layer on the outer surface was efficient to inhibit the swelling of the capsule membranes. The immobilizing yield was considered to be 100% because all the enzyme molecules were encapsulated inside the capsules through the proposed method, and the laccase activity immobilized in APSi capsules was 61.8 mmol·g-1·min-1. The thermal, pH and storage stabilities of the immobilized laccase in APSi capsules were determined in comparison with free laccase. The stability of encapsulated laccase was significantly improved, which was as high as 67% after 20 days. The residual relative activity of encapsulated laccase remained 45% after 10 cycles. © 2012 American Institute of Chemical Engineers (AIChE). Source

Lu T.,Chengdu Rongsheng Pharmaceuticals Co. | Mu L.,Chengdu Rongsheng Pharmaceuticals Co. | Liu B.,Chengdu Rongsheng Pharmaceuticals Co. | Wang Y.,Chengdu Rongsheng Pharmaceuticals Co.
Chinese Journal of Biologicals

Objective To prepare and identify a novel intravenous immunoglobulin (IVIG). Methods Eight batches of 10% IVIG were prepared by ultrafiltration. Glycine was used as a stabilizer instead of sugar, of which the concentration was optimized (12 ∼ 18 g/L). The 10% IVIG was tested for overall quality, IgG subclass distribution, Fc activity and stability. Results The optimal concentration of glycine was 15 g/L. The overall quality of 10% IVIG met the relevant requirements, while the IgG subclass distribution, Fc activity and stability were similar to those of 5% IVIG. The overall quality of 10% IVIG met the relevant requirements after storage at 25°c for 6 months and at 2 ∼ 8°C for 6 years. Conclusion The 10% IVIG showed good quality and stability. Source

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