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Xu C.-H.,Tsinghua University | Liu S.-L.,Tsinghua University | Zhao S.-N.,Chengde Medical University | Li S.-Z.,Chengde Medical University | Sun S.-Q.,Tsinghua University
Planta Medica | Year: 2013

Given that harvesting time has a great impact on the quality of herbal medicine, knowing the ontogenesis in the chemical profile aspect is essential to determine the optimal harvesting season. A high-throughput and versatile approach (herbal infrared macro-fingerprinting) harmonizing with the character of herbal medicine and providing the whole chemical profile (entirety), group analogues (part), and single compounds (major components) is developed to rapidly disclose the variation rule of the full chemical profile of herbal medicine over a growing season without extraction pretreatments, and thus to determine the optimal harvesting period in respect to groups of chemical compounds using Scutellaria baicalensis as a demonstration. IR macro-fingerprints of Scutellaria baicalensis harvested in the same period have a high similarity (> 0.91) despite small variations, suggesting that IR macro-fingerprinting can faithfully reflect the spectacle of "disordered ordero" in nature. From Year-1 spring to Year-3 autumn, general contents (%, w/w) of total flavonoids fluctuate up and down with a maximum value in Year-2 spring, and that of saccharides is relatively stable except for the attenuation from Year-2 autumn to Year-3 spring. From Year-1 autumn to Year-2 spring, flavonoid aglycones initially produced in Scutellaria baicalensis are extensively transformed to responding flavonoid glycosides. From Year-2 spring to Year-3 autumn, flavonoid glycosides are converted back to their corresponding aglycones. The best seasons for collecting Scutellaria baicalensis with a high content of flavonoid glycosides and aglycones would be Year-2 spring and Year-3 spring, respectively. © Georg Thieme Verlag KG Stuttgart New York.


Xian-Hui D.,Chengde Medical University | Xiao-Ping H.,266th Hospital of Peoples Liberation Army | Wei-Juan G.,Hebei University
Journal of Spinal Cord Medicine | Year: 2016

Background: The Buyang Huanwu decoction (BYHWD) is a traditional Chinese herbal prescription and has been used in China to treat spinal cord injury (SCI) for hundreds of years. Clinical trials have shown that BYHWD improves the outcome of SCI in clinical trials, but the mechanisms are not known. This study observed the neuroprotective effects of BYHWD on spinal nerve cells after SCI and investigated possible mechanisms. Materials and methods: Forty female Wistar rats were randomized equally to four groups treated by sham injury, SCI, BYHWD, or methylprednisolone (MP). The Basso, Beattie, and Bresnahan (BBB) score was used to evaluate hind-limb locomotor function. Neuron apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling staining and caspase-3, Bax, and Bcl-2 mRNA and protein expression were evaluated by real-Time quantitative polymerase chain reaction and Western blotting, respectively. Results: In the sham group, walking was mildly abnormal after anesthesia but recovered completely in 2 days. The BBB score in the SCI model group was significantly different from that in the sham group. The BBB scores of rats in both the BYHWD and MP groups were significantly higher than scores of rats in the SCI group. BYHWD had an antiapoptosis effect, as shown by significant decreases in expression of caspase-3 and Bax and increase in Bcl-2 expression. Conclusion: BYHWD treatment restored hind-limb motor function of rats with SCI. The neuroprotective effect of BYHWD was associated with modulation of the expression of apoptosis-related proteins. © 2015 The Academy of Spinal Cord Injury Professionals, Inc.


Dong X.-H.,Chengde Medical University | Gao W.-J.,Chengde Medical University | He X.-P.,Chengde Medical University
International Journal of Ophthalmology | Year: 2012

AIM: To evaluate the efficacy of topical administration Natamycin, which is produced by China, in an experimental rabbit model of Fusarium solani keratitis, to provide experimental basis for the application of clinical safety. METHODS: Fusarium solanio was induced in the right eye of 30 New Zealand rabbits. Forty-eight hours after inoculation, the animals were divided into 3 different treatment groups, 10 rabbit eyes of each group: Group 1 (Natamycin) treated with topical Natamycin, group 2 (Natacyn) treated with topical Natacy n, group 3 (control) treated with topical saline solution. The eyes of each group was examined clinically with slit lam pusing ulcer scoring system on day 4, 10, 15, and 21 for status of healing, corneal vascularisation, iritis, hypopy on and macular nebula. The findings were recorded on day 10 and day 21. RESULTS: Ulcer score on day 10, day 15, day 21: The score of Natamy cin group are 1.45 ±0.16, 1.08 ±0.11, 0.70±0.40. The score of Natacyn group are 1.35 ±0.12, 1.10 ±0.12, 0.65 ±0.35. the score of control group are 1.30 ±0.08, 3.63 ±0.28, 3.80 ±0.16. Natamycin group and Natacyn group were different from control group (P<0.01). There is no difference between Natamycin group and Natacyn group. Status of healing on day 10 and day 21: The cure rate of the Natamycin group is 90% on day 10, and 100% on day 21. The cure rate of the Natacyn group is 80% on day 10, and 100% on day 21. Natamy cin group and Natacyn group were different from control group (P<0.01). There is no difference between Natamycin group and Natacyn group. Corneal vascularisation, iritis, hypopyon and macular nebula on day 10 and day 21: in Natamycin group, the number of the eyes which have Corner vascularisation, iritis, hypopyon and macular nebula are 2,0,0,2. In Natacyn group, the number of the eyes which have Corner vascularisation, iritis, hypopy on and macular nebula are 1,0,0,2. In control group, the number of the eyes which have Corner vascularisation, iritis, hypopyon and macular nebula are 9,9,8,9. Natamycin group and Natacyn group were different from control group (P<0.01). There is no difference between Natamycin group and Natacyn group. CONCLUSION: Natamycin was found tobe effective in fungal keratitis, similar to Natacyn, and it can stop the corner vascularisation, iritis, hypopyon and macular nebula to happen. Natamyin manufactured in China is effective against fungal keratitis, with esay availability and low toxicity in its use. Copyright International Journal of Ophthalmology Press.


Sun N.-X.,Chengde Medical University | Gao W.-J.,Chengde Medical University | Gao W.-J.,Hebei Chemical and Pharmaceutical College | Yi Z.-L.,Chengde Medical University
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: During the progress of focal cerebral ischemia-reperfusion in rat with transient suture occlusion, bleeding combating and the line being plugged into the cerebral middle artery smoothly are important for successful modeling and research focus. Objective: To improve Longa suture occlusion method for focal cerebral ischemia reperfusion model in Sprague-Dawley rats in order to establish a simple surgical procedure with low rate of intraoperative bleeding and high rate of successful models. Methods: The two ends of the external carotid artery were clipped with electric knife, in addition to ligation of surgery lines intraoperatively; with the help of pincett, a fishing line was plugged into the carotid artery along the anteriomedialis artery wall (the direction of the internal carotid artery from the pterygopalatine arterial bifurcation). Postoperative behavior scores in rats, infarct volume calculation and histopathological evaluation under optical microscope were done. Results And Conclusion: Transverse sections of the external carotid artery were changed from a "O" shape to a "-" shape after being clipped with electric knife. Subsequently, line nodes were not easy to fall off any more, effectively preventing intraoperative bleeding. With the power of pincett, the rate of plugging the fishing line into artery was significantly increased. Rat's neurological score and infarct volumes were significantly increased, and brain tissue pathological injury worsened. The modified suture occlusion method for focal cerebral ischemia reperfusion models effectively prevented line nodes off, successfully increased the rate of suture occlusion and models in Sprague-Dawley rats were developed successfully.


Yida Z.,University Putra Malaysia | Yida Z.,Chengde Medical University | Imam M.U.,Chengde Medical University | Ismail M.,University Putra Malaysia
BMC Complementary and Alternative Medicine | Year: 2014

Background: Edible birds' nest (EBN) is reported to be antioxidant-rich. However, the fate of its antioxidants after oral consumption is not yet reported. To explore this, we hypothesized that EBN antioxidants are released from their matrix when subjected to in vitro simulated gastrointestinal digestion. Methods: EBN samples were extracted using hot water (100°C) with or without subsequent sequential enzymatic digestion using pepsin (10,000 units), pancreatin (36 mg) and bile extracts (112.5 mg). Additionally, pH changes (8.9 to 2 and back to 8.9) similar to the gut were applied, and a 10 KDa dialysis tubing was used to simulate gut absorption. The antioxidant capacities of the water extracts of EBN before and after digestion were then determined using ABTS and oxygen radical absorbance capacity (ORAC) assays, while the protective effects of the EBN samples against hydrogen peroxide-induced toxicity in HEPG2 cells were determined using MTT assay and acridine orange (AO)/propidium iodide (PI) staining. Results: Antioxidant assays (ABTS and ORAC) showed that the undigested EBN water extract had little antioxidant activity (1 and 1%, respectively at 1000 μg/mL) while at similar concentrations the digested samples had significantly (p < 0.05) enhanced antioxidant activities, for samples inside (38 and 50%, respectively at 1000 μg/mL) and outside (36 and 50%, respectively at 1000 μg/mL) the dialysis tubing, representing absorbed and unabsorbed samples, respectively. Cell viability and toxicity assays also suggested that the EBN extracts were non-toxic to HEPG2 cells (cell viabilities of over 80% at 1000 μg/mL), while AOPI showed that the extracts protected HEPG2 cells from hydrogen peroxide induced-toxicity. Conclusions: Based on the findings, it is likely that EBN bioactives are released from their matrix when digested in the gut and then absorbed through the gut by passive-mediated transport to exert their functional effects. However, there is need to confirm these findings using in vivo systems to determine their clinical significance. © 2014 Yida et al.


Li J.,Zhejiang University | Li J.,Chengde Medical University | Cai Y.,CAS Beijing Institute of Genomics | Ye L.,Zhejiang University | And 5 more authors.
BMC Genomics | Year: 2014

Background: The growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production.Results: Here, aided by next-generation sequencing and microarry assay, we profile 1,229 microRNAs (miRNAs), including 728 novel miRNAs and 110 miRNA/miRNA* duplexes, of the posterior silk gland at the fifth larval instar. Target gene prediction yields 14,222 unique target genes from 1,195 miRNAs. Functional categorization classifies the targets into complex pathways that include both cellular and metabolic processes, especially protein synthesis and processing.Conclusion: The enrichment of target genes in the ribosome-related pathway indicates that miRNAs may directly regulate translation. Our findings pave a way for further functional elucidation of these miRNAs and their targets in silk production. © 2014 Li et al.; licensee BioMed Central Ltd.


Wang X.-J.,Chengde Medical University | Li X.,Chengde Medical University
Acta Anatomica Sinica | Year: 2013

Objective: To study whether annexin A7(ANXA7) low expression can affect proliferation of HepG2 cells. Methods: Western blotting was used to identify siRNA can highly surpress annexin A7 expression. When the effect of the siRNA was assured, the siRNA was transfected into HepG2 cells by lipofectamine transfection method. At the same time, cells in negative control group were transfected negative siRNA while cells in blank control group only added lipofectamine 2000. Twenty-four, forty-eight and seventy-two hours after transfection, cell counting was used to draw growth curve. Forty-eight hours after transfection, MTT assay was used to detect proliferation activity; Immunohistochemistry was applied to observe the expression of cyclinD1 and Ki67; Western blotting was performed to detect the expression of cyclinD1. Results: Cell counting and MTT assay showed that proliferation of the siRNA interference group decreased significantly(P < 0.05). The expression of cyclinD1 and Ki67 decreased compared with the negative control group and the blank control group(P < 0.05). Conclusion: ANXA7 low expression may inhibit proliferation of HepG2 cells.


The present invention relates to the field of pharmaceutical chemistry, and particularly to a novel class of benzazepine ketone derivatives (I), their preparation method and their pharmaceutical uses. The compounds have glycogen phosphorylase inhibiting effect and can be used in the preparation of anti-diabetes and its complications medicaments, anti-cerebral ischemia medicaments, anti-cardiovascular diseases medicaments, blood lipid-lowering medicaments, weight-reducing medicaments, anti-atherosclerosis medicaments, medicaments for treating metabolic syndrome or anti-tumor medicaments. The present invention also relates to a preparation method of the class of compounds and pharmaceutical formulations containing the same.


Trichinella spiralis (T.spiralis) muscle-larva (ML) excretory-secretory proteins (ESPs) contain antitumour-active substances. ESPs have been shown to inhibit tumour growth. To explore the effects of these proteins on small cell lung cancer cells and the possible mechanisms of their antineoplastic action, H446 SCLC cells were co-cultured with different concentrations of T. spiralis ML ESPs for 12, 24 and 48 h. Our results showed that T. spiralis ML ESPs significantly inhibited H446 cell proliferation, which was dose-and time-dependent. The results of flow cytometry testing indicate a clear apoptosis trend in H446 cells co-cultured with ESPs for 24 h. Reverse transcription polymerase chain reaction and Western blotting results showed increased expression of pro-apoptosis genes Bax, Cyt-C, Apaf-1, caspase-9 and caspase-3, compared with the negative control group, and decreased the expression of anti-apoptosis genes Bcl-2 and Livin. Our results suggest that T. spiralis ML ESPs can induce apoptosis in H446 cells through a mitochondrial pathway, which may be a mechanism of antineoplastic action in T. spiralis ML ESPs.


PubMed | Chengde Medical University
Type: | Journal: Molecular and cellular biochemistry | Year: 2017

High expression of connexins was found in a variety of cancers, but their role is still controversial. We investigated whether connexin43 (Cx43) contributed to bladder carcinogenesis through MAPK activation. In this study, we found that Cx43 expression was significantly increased in bladder cancer tissues and cell line. Overexpression of Cx43 in bladder cancer 5637 cells increased cell proliferation, promoted cell cycle progression, and inhibited apoptosis. Western blot showed that JNK and ERK pathways were dramatically activated in Cx43-overexpressed cells. Conversely, knockdown of Cx43 inhibited cell proliferation by increasing apoptosis and causing cell cycle arrest, concomitant with inhibition of JNK and ERK signaling. In addition, JNK and ERK pathways were also activated in bladder cancer tissues. In conclusion, abnormal high expression and cytoplasmic localization of Cx43 contributed to bladder cancer. Inhibition of Cx43 activity could be a potential therapeutic strategy for preventing the progression of bladder cancer.

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