Wang L.-Z.,Chemoradiotherapy Center |
Zhang H.-J.,Chengde Central Hospital |
Song J.,Chemoradiotherapy Center
Asian Pacific Journal of Cancer Prevention | Year: 2015
Objective: To evaluate the efficacy of mannatide combined with sodium cantharidate vitamin B6 in the treatment of malignant pleural effusions. Materials and Methods: Data for 69 patients with malignant pleural effusions who did not receive systemic chemotherapy were collected. Injection into the thorax using mannatide combined with sodium cantharidate vitamin B6 was performed for 37 patients in the experimental group and mannatide combined with cisplatin for 32 patients in the control group. Objective responses, KPS (Karnofsky Scoring) and incidences of side effects between the two groups were compared. Results: 13 patients reached CR (complete response) and 11 PR (partial response) in the experimental group, while 12 patients reached CR and 9 PR in the control group, the difference in overall objective responses between the two groups not being significant (66.7% vs 63.6%, p=0.806). However, improvement of KPS in the experimental group wasgreater than in the control group; total side-effect incidences during the period of treatment were 22.2% (8/36) and 54.5% (18/33), respectively (p=0.006). Conclusions: Regimen of mannatide combined with sodium cantharidate vitamin B6 had better improvement in quality-of-life and symptom relief, with a lower side-effect incidence in treatment of malignant pleural effusions.
Xu F.,Nanjing Medical University |
Yu H.,Chengde Central Hospital |
Liu J.,Nanjing Medical University |
Cheng L.,Nanjing Medical University
Molecular Biology Reports | Year: 2013
The present study was carried out to observe the protective effects of αB-crystallin protein on hydrogen peroxide (H2O 2)-induced injury in rat myocardial cells (H9c2) and to investigate the mechanisms of these protective effects at the cellular level, which could provide the experimental basis for future applications of αB-crystallin in the treatment of cardiovascular disease. Western blotting was used to measure the expression of αB-crystallin in cultured H9c2 cells in vitro. A αB-crystallin recombinant expression vector, pcDNA3.1-Cryab, was constructed to transfect H9c2 cells for the establishment of cells that stably expressed αB-crystallin. A tetrazolium-based colorimetric assay (MTT test) was used to measure changes in the viability of the H9c2 cells at 1, 2, 3 and 4 h after induced by 150 μM H2O2 to establish a model of H2O2 injury to cells. H2O2 was applied to H9c2 cells that were stably transfected with αB-crystallin, and the effect of αB-crystallin overexpression on the viability of myocardial cells subjected to H2O2-induced injury was measured by the MTT assay. The effect of αB-crystallin overexpression on the H 2O2-induced injury of H9c2 cells was also analyzed by flow cytometry. The mitochondrial components and cytoplasmic components of H9c2 cells were separated, and western blotting was used to measure the effect of αB-crystallin overexpression on the release of cytochrome c from the mitochondria. Western blotting was also used to measure the effect of αB-crystallin overexpression on the expression of the anti-apoptosis protein Bcl-2 and components of the phosphatidylinositol 3-OH kinase (PI3K)/AKT pathway. The αB-crystallin recombinant expression vector pcDNA3.1-Cryab successfully transfected H9c2 cells, and H9c2 cells that were stably transfected with αB-crystallin were established after G418 selection. The measurements carried out by western blotting showed that αB-crystallin proteins are expressed in normal H9c2 cells, but the proteins' expression was much higher in pcDNA3.1-Cryab transfected cells (P < 0.01). The MTT assays showed that 4 h of H2O2 treatment induced significant injury in H9c2 cells (P < 0.01), but αB-crystallin overexpression can effectively antagonize the H2O2-induced injury to H9c2 cells (P < 0.05). The results of flow cytometry analysis showed that αB-crystallin overexpression can significantly reduce apoptosis in H 2O2-injured H9c2 cells (P < 0.05). The results of western blotting showed that αB-crystallin overexpression in myocardial cells can reduce the H2O2-induced release of cytochrome c from the mitochondria (P < 0.05), antagonize the H2O 2-induced downregulation of Bcl-2 (P < 0.05) and magnify the decrease in phosphorylated AKT levels induced by H2O2 injury (P < 0.05). The overexpression of αB-crystallin has a protective effect on H2O2-injured H9c2 cells, and αB-crystallin can play a protective role by reducing apoptosis, reducing the release of cytochrome c from the mitochondria and antagonizing the downregulation of Bcl-2 expression. The protective effects of αB-crystallin may be related to the PI3K/AKT pathway. © 2012 Springer Science+Business Media Dordrecht.
Zhang X.-M.,Peking Union Medical College |
Wang Z.,Peking Union Medical College |
Ma S.-H.,Chengde Central Hospital |
Zhou Z.-X.,Peking Union Medical College
Asian Pacific Journal of Cancer Prevention | Year: 2014
Background: Surgery offers the only potential for cure and long-term survival of recurrence of rectal cancer. Few studies about laparoscopic recurrent lesion resection have been reported. This study was designed to evaluate the safety and feasibility of laparoscopic abdomino-perineal resection for anastomotic recurrence of rectal cancer. Materials and Methods: Data for 42 patients with recurrence of rectal cancer were collected retrospectively. Of the 42 patients, 22 underwent laparoscopic surgery (LR group) and 20 received open surgery (OR group). Outcomes between the two groups were compared. Results: Operation time in LR group was shorter compared with the OR group (164.6±27.7min vs 203.0±45.3min); intra-operative blood loss was 119.7±44.4ml and 185.0±94.0ml in LR group and OR group, respectively (p < 0.001); time to first flatus in LR group was shorter than in OR group, and the difference was statistically significant (2.6±0.8 days vs 3.1±0.8 days, p=0.013); hospital stay in the LR and OR groups was 8.6±1.3 days and 9.8±2.2 days; 3-year survival rates in the LR and OR groups were 44.4% and 42.8% (p=0.915) and the 3-year disease-free survival rates were 36.4% and 30.0%, respectively (p=0.737). Conclusions: Laparoscopic abdomino-perineal resection is safe and feasible for anastomotic recurrence of rectal cancer.
Zhang H.-C.,Dalian Medical University |
Dai J.-L.,Chengde Central Hospital |
Zhao Y.,Dalian Medical University
Journal of Dalian Medical University | Year: 2012
[Objective] To explore clinical effects of Paravertebral block (PVB) combined with morphine patient-controlled intravenous analgesia(PCIA) in the treatment of breakthrough cancer pain(BTCP). [Method] For the clinical stage IV cancer patients of poor analgesic effect by routine application of morphine, the combined analgesic method was been used, to observe pain intensity (PI) and the degree of pain relief (PAR), and record the frequency of BTCP. [Result] Before the combined analgesic method, the average interval time(hour) of BTCP and the number of occurrence frequency within 24 hours were 9.29 ± 3.6, 3.21 ± 1.26, after then were 24.69 ± 8.09, 0.86 ± 0.74. There were significant differences (P < 0.05) ;The pain score before the combined analgesic method was 8.89 ± 0.817, after then was 1.79 ± 1.677. There were significant differences (P < 0.05). [Conclusion] The treatment of PVB combined with PCIA could significantly reduce the frequency of BTCP in the advanced cancer patients with severe pain, and improve their quality of life. For the more, it was the feasibility of clinical application.
Duan X.G.,Chengde Central Hospital
Zhonghua nan ke xue = National journal of andrology | Year: 2013
Erectile dysfunction (ED) is a common male disease and frequently encountered in Andrology. PDE5 inhibiter can competitively inhibit the hydrolysis of cyclic guanosine monophosphate (cGMP), increase the cGMP concentration in the smooth muscle cells of the corpus cavernosum penis, and achieve the therapeutic effect on ED. Tadalafil can elevate the level of cGMP in the corpus cavernosum and effectively improve ED of various causes and degrees. Tadalafil therapy for ED, characterized by long efficacy, high safety, easy acceptance by patients and their partners, and obvious promotion of patients'self-confidence and natural experience in sexual activities, can achieve satisfactory physiological and psychological results and effectively improve the quality of patients' sexual life. Therefore tadalafil deserves an extensive clinical application in the treatment of ED.