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Muri, Switzerland

Pittet G.,Suisselab AG | Luginbuhl W.,ChemStat | Berger T.,Forschungsanstalt Agroscope Liebefeld Posieux ALP Haras
Agrarforschung Schweiz | Year: 2012

Every month, only one official milk-testing result is available from between 1000 and 1200 milk producers instead of the two required. Of the missing results, one part originates from milk-collection centers where milk delivery takes place every other day and sampling is performed manually, and is therefore attributable to the time delay between sampling notification and milk delivery. Extending the maximum time allowed between sampling and commencement of the analysis from 30 to 36 hours would provide an easy and cost-effective method to solve this problem. The increase in the number of samples with results above the maximum permitted limit would be slight: In the present study, the percentage increased from 1,397 % at 30 hours to 1,468 - 2,648 % at 36 hours, representing a rise of 0,07 - 1,25 %. This can be accepted as a justifiable drawback as it would affect very few producers and only those whose milk at 30 hours contains bacterial counts close to the official limit. The overall discrimination would probably be even lower as the increased time span would not necessarily affect both monthly samples and therefore, after calculation of the monthly geometric mean as required by the public-law milk quality control, the results may well remain within the limits set.

Schmid A.,Institute for Food science IFS | Petry N.,Institute for Food science IFS | Petry N.,GroundWork | Walther B.,Institute for Food science IFS | And 10 more authors.
British Journal of Nutrition | Year: 2015

Postprandial inflammation is an important factor for human health since chronic low-grade inflammation is associated with chronic diseases. Dairy products have a weak but significant anti-inflammatory effect on postprandial inflammation. The objective of the present study was to compare the effect of a high-fat dairy meal (HFD meal), a high-fat non-dairy meal supplemented with milk (HFM meal) and a high-fat non-dairy control meal (HFC meal) on postprandial inflammatory and metabolic responses in healthy men. A cross-over study was conducted in nineteen male subjects. Blood samples were collected before and 1, 2, 4 and 6 h after consumption of the test meals. Plasma concentrations of insulin, glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol, TAG and C-reactive protein (CRP) were measured at each time point. IL-6, TNF-α and endotoxin concentrations were assessed at baseline and endpoint (6 h). Time-dependent curves of these metabolic parameters were plotted, and the net incremental AUC were found to be significantly higher for TAG and lower for CRP after consumption of the HFM meal compared with the HFD meal; however, the HFM and HFD meals were not different from the HFC meal. Alterations in IL-6, TNF-α and endotoxin concentrations were not significantly different between the test meals. The results suggest that full-fat milk and dairy products (cheese and butter) have no significant impact on the inflammatory response to a high-fat meal. Copyright © The Authors 2015.

Pauly C.,Agroscope Liebefeld Posieux Research Station | Luginbuhl W.,ChemStat | Ampuero S.,Agroscope Liebefeld Posieux Research Station | Bee G.,Agroscope Liebefeld Posieux Research Station
Meat Science | Year: 2012

Alternatives to the common castration (C) practice of piglets are surgical castration under anaesthesia and rearing entire males (EM) or immunocastrates (IC). It is well established that boar taint hinders the breakthrough of these options. Less is known how avoiding surgical castration would affect carcass characteristics and pork quality. The objective of this meta-analysis was to estimate the impact of lack of castration on quality traits besides boar taint. The most marked effect of castration method and gender was found in lean meat and intramuscular fat percentage. Compared to EM, carcass leanness was estimated to be greater (P< 0.05) and intramuscular fat level lower (P< 0.05) than in C, IC and females. Regarding pork quality traits only the difference in shear force between IC and EM was of relevant magnitude. This meta-analysis revealed that the implementation of EM production should not be hindered by pork quality concerns. © 2012 Elsevier Ltd.

Harju K.,University of Helsinki | Rapinoja M.-L.,University of Helsinki | Avondet M.-A.,Spiez Laboratory | Arnold W.,Spiez Laboratory | And 8 more authors.
Toxins | Year: 2015

A saxitoxin (STX) proficiency test (PT) was organized as part of the Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk (EQuATox) project. The aim of this PT was to provide an evaluation of existing methods and the European laboratories’ capabilities for the analysis of STX and some of its analogues in real samples. Homogenized mussel material and algal cell materials containing paralytic shellfish poisoning (PSP) toxins were produced as reference sample matrices. The reference material was characterized using various analytical methods. Acidified algal extract samples at two concentration levels were prepared from a bulk culture of PSP toxins producing dinoflagellate Alexandrium ostenfeldii. The homogeneity and stability of the prepared PT samples were studied and found to be fit-for-purpose. Thereafter, eight STX PT samples were sent to ten participating laboratories from eight countries. The PT offered the participating laboratories the possibility to assess their performance regarding the qualitative and quantitative detection of PSP toxins. Various techniques such as official Association of Official Analytical Chemists (AOAC) methods, immunoassays, and liquid chromatography-mass spectrometry were used for sample analyses © 2015 by the authors; licensee MDPI, Basel, Switzerland.

Worbs S.,Robert Koch Institute | Fiebig U.,Robert Koch Institute | Zeleny R.,European Commission | Schimmel H.,European Commission | And 3 more authors.
Toxins | Year: 2015

In the framework of the EU project EQuATox, a first international proficiency test (PT) on the detection and quantification of botulinum neurotoxins (BoNT) was conducted. Sample materials included BoNT serotypes A, B and E spiked into buffer, milk, meat extract and serum. Different methods were applied by the participants combining different principles of detection, identification and quantification. Based on qualitative assays, 95% of all results reported were correct. Successful strategies for BoNT detection were based on a combination of complementary immunological, MS-based and functional methods or on suitable functional in vivo/in vitro approaches (mouse bioassay, hemidiaphragm assay and Endopep-MS assay). Quantification of BoNT/A, BoNT/B and BoNT/E was performed by 48% of participating laboratories. It turned out that precise quantification of BoNT was difficult, resulting in a substantial scatter of quantitative data. This was especially true for results obtained by the mouse bioassay which is currently considered as “gold standard” for BoNT detection. The results clearly demonstrate the urgent need for certified BoNT reference materials and the development of methods replacing animal testing. In this context, the BoNT PT provided the valuable information that both the Endopep-MS assay and the hemidiaphragm assay delivered quantitative results superior to the mouse bioassay. © 2015 by the authors; licensee MDPI, Basel, Switzerland.

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