Chemisches und Veterinaruntersuchungsamt Stuttgart

Fellbach, Germany

Chemisches und Veterinaruntersuchungsamt Stuttgart

Fellbach, Germany
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Johler S.,University of Zürich | Tichaczek-Dischinger P.S.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Rau J.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Sihto H.-M.,University of Zürich | And 3 more authors.
Foodborne Pathogens and Disease | Year: 2013

In 2008, 150 people gathered for a wedding celebration in Baden-Württemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy. Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transform-infrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determine the presence/absence of genes involved in virulence and antimicrobial resistance. We were able to match an enterotoxigenic strain from the stool sample of a patient to isolates of the same strain obtained from food and the nasal cavity of a food handler. The strain produced the enterotoxin SEA and the toxic shock syndrome toxin-1, and was also found to exhibit the genes encoding enterotoxins SEG and SEI, as well as the enterotoxin gene cluster egc. This is one of only a few studies that were able to link a staphylococcal food poisoning outbreak to its source. © 2013, Mary Ann Liebert, Inc.

Agency: European Commission | Branch: FP7 | Program: CP-IP | Phase: KBBE-2007-2-4-02 | Award Amount: 7.51M | Year: 2008

RASFF alerts show that monitoring of chemical contaminants in food and feed is very relevant in European food safety. Also consumers placed chemical contaminants on top of the worry-scale of food-related risks. According to the General Food Law, food and feed industries are responsible for the safety of their products. Often expensive instrumental single-analyte methods are being applied by regulatory and industrial laboratories. There is an urgent need for replacement by validated screening tools which are simple, inexpensive and rapid, but also show multiplex capability by detecting as many contaminants in parallel as possible. The CONffIDENCE proposal has been designed to provide long-term solutions to the monitoring of persistent organic pollutants, perfluorinated compounds, pesticides, veterinary pharmaceuticals (coccidiostats, antibiotics), heavy metals and biotoxins (alkaloids, marine toxins, mycotoxins) in high-risk products such as fish and fish feed, cereal-based food/feed and vegetables. A balanced mix of novel multiplex technologies will be utilized, including dipsticks, flow cytometry with functionalised beads, SPR optical and electrochemical biosensors, cytosensors and metabolomics-like comprehensive profiling. After validation, the simplified methods will be applied in impact demonstrators that contribute to exposure assessment and validation of hazard models. Moreover, hazards of emerging contaminants will be assessed through toxicological testing. Dissemination to scientists and to relevant stakeholders, including the food and feed industry, regulatory control bodies, DG-SANCO, EFSA, exporting countries, CRLs, routine laboratories, CEN and consumers will be assured by e-communication, press releases, public workshops, open days, presentations, publications and a science education module. The consortium consists of 17 partners from 10 countries, representing 9 research institutes, 5 universities, 2 large food and feed industries and 1 SME.

Kolberg D.I.S.,Chemisches und Veterinaruntersuchungsamt Stuttgart | MacK D.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Anastassiades M.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Hetmanski M.T.,UK Environment Agency | And 3 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

A new sensitive, fast and robust method for the determination of paraquat and diquat residues in potatoes, cereals and pulses is presented. Different extraction conditions (solvent, time and temperature) have been evaluated using barley grain, potatoes and dry lentils containing incurred residues of diquat and paraquat. The finalised procedure involves extraction with a mixture of methanol/water/ hydrochloric acid at 80 °C and analysis by liquid chromatography- tandem mass spectrometry. Diquat D4 and Paraquat D6 internal standards were added to the test portions prior to extraction. A small-scale inter-laboratory validation of the developed method for diquat and paraquat using potato and barley samples was conducted by three laboratories. The precision and accuracy of the method were determined from recovery experiments (five replicates) at 0.01 and 0.1 mgkg-1. The recoveries obtained (n=180) were in the range of 92-120 % with associated relative standard deviation (RSD) between 1.4-10 % for all compound/ commodity/spiking concentration combinations. © Springer-Verlag 2012.

Lohrey L.,University of Munster | Marschik S.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Cramer B.,University of Munster | Humpf H.-U.,University of Munster
Journal of Agricultural and Food Chemistry | Year: 2013

Tenuazonic acid is a fungal secondary metabolite that is produced by a number of Alternaria species and is therefore a natural contaminant of food and feed samples. This paper describes a new strategy for the efficient and economical large-scale synthesis of the isotopically labeled internal standard 13C2-tenuazonic acid via a three-step procedure. Furthermore, a new reliable and quick method based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) cleanup is presented for the determination of tenuazonic acid in food and feed samples utilizing high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) by application of the stable isotope dilution analysis. This new method has a limit of detection (LOD) of 0.86 μg/kg and a limit of quantitation (LOQ) of 2.89 μg/kg. In total 26 tomato samples and 4 bell pepper samples from the German market were analyzed. Tenuazonic acid was found in each sample with levels from 3 to 2330 μg/kg. © 2012 American Chemical Society.

Weisshaar R.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Perz R.,Chemisches und Veterinaruntersuchungsamt Stuttgart
European Journal of Lipid Science and Technology | Year: 2010

Discrepancies in the analysis of 3-chloropropane-1,2-diol (3-MCPD) esters can be explained by the hypothesis that in some refined oils significant amounts of fatty acid esters of glycidol (glycidyl esters) are present in addition to 3-MCPD esters. Glycidyl esters were separated from triacylglycerols by gel permeation chromatography (GPC) and detected by gas chromatography-mass spectrometry (GC-MS). Six samples of palm oil and palm oil-based fats were analyzed by GPC and GC-MS. In chromatograms of all samples, significant peaks, retention time and mass spectra in conformity with self-synthesized glycidyl palmitate and glycidyl oleate were detectable. Quantification of individual glycidyl esters was not possible because of a lack of pure standards. Concentration of ester-bound glycidol in different samples of fats and oils was estimated using an indirect difference method. Glycidyl esters could be detected only in refined, but not in crude or native, fats and oils. The highest concentrations were detected in palm oil and palm oil-based fats. In a palm oil sample, glycidyl ester concentration varied according to different deodorization parameters, temperature, and time, while 3-MCPD ester concentration was relatively constant, indicating that mitigation of glycidyl esters possibly may be achieved by optimizing refining parameters. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Sting R.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Richter A.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Popp C.,Poultry Health Service | Hafez H.M.,Free University of Berlin
Poultry Science | Year: 2013

In the present study, the prevalence of vancomycin-resistant enterococci (VRE) in turkeys in the southwest of Germany was investigated. For this purpose, 200 cloacal swab samples and 5 environmental dust samples (tested as a pooled sample) of each of the 20 flocks (10 female and 10 male flocks) included in this study were examined. The VRE could be isolated by means of a procedure combining bacterial cultivation in an enrichment broth and on a selective solid media. Enterococci were identified biochemically and subsequently tested on the presence of the vancomycin resistance genes vanA, vanB (B1/B2/B3), and vanC (C1/ C2/C3) using real-time PCR assays. In 54 (27%) turkeys originating from 11 (55%) flocks and in 14 (70%) of the dust samples, exclusively vanA and vanC1 genes could be detected. Of the turkeys examined, 46 were colonized with VRE bearing the resistance gene vanC1 and 8 vanA, originating from 9 and 2 flocks, respectively. None of the birds carried vanB, vanC2, or vanC3 positive VRE. The results obtained from the birds are largely confirmed by the dust samples originating from 4 vanA and 10 vanC1 positive flocks. However, one flock housing animals colonized with vanC1 positive VRE could not be confirmed by the dust samples that revealed vanA bearing VRE. However, in one case vanA and in 3 cases vanC1 carrying VRE could be detected in dust samples of the turkey houses, but not in the turkeys of the associated flock. In 5 flocks the turkeys as well as the dust samples were free of VRE. © 2013 Poultry Science Association Inc.

Vollmer A.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Biedermann M.,Official Food Control Authority of the Canton of Zurich | Grundbock F.,Official Food Control Authority of the Canton of Zurich | Ingenhoff J.-E.,Official Food Control Authority of the Canton of Zurich | And 3 more authors.
European Food Research and Technology | Year: 2011

From the German market, 119 samples of dry food were analyzed for the migration of mineral oil. The products selected were packed in paperboard boxes and intended for storage for extended periods of time at ambient temperature. The 0.6 mg/kg limit for mineral oil saturated hydrocarbons (MOSH) derived from the WHO/JECFA evaluation was frequently exceeded by a factor of 10-100. Typically, 10-20% of the migrating mineral oil consisted of aromatic hydrocarbons (MOAH). Most samples were merely 2-3 months old and far from the end of their shelf life (usually 1-3 years). From the assumption that about 70% of the MOSH and MOAH which are eluted from GC up to the C 24 n-alkane (

Pantchev A.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Sting R.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Bauerfeind R.,Justus Liebig University | Tyczka J.,Chemisches und Veterinaruntersuchungsamt Karlsruhe | Sachse K.,Friedrich Loeffler Institute Jena
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2010

The aim of the present study was to analyse the occurrence of chlamydiae in several mammalian host species. Clinical samples that previously tested positive in a Chlamydiaceae-specific real-time PCR were retested using six species-specific real-time PCR assays to identify the chlamydial species involved. Chlamydophila (Cp.) abortus was the agent most frequently found in cattle, sheep, horses, goats, and pigs. Detection in cattle of Cp. psittaci (11% of samples) and Chlamydia (C.) suis (9%), as well as Cp. psittaci in a goat sample was somewhat unexpected. DNA of two different chlamydiae was identified in 56 (12.7%) of 440 samples tested. Cp. felis was the predominant species found in cats, while in guinea pigs and rabbits only Cp. caviae was detected. Interestingly, the latter two pathogens were also identified in samples from dogs. The data show that mixed chlamydial infections are not rare and suggest an extended host range of individual species. L'objectif de la présente étude est d'analyser l'occurrence de chlamydiae et ce, dans plusieurs espèces mammaliennes hôtes. Des échantillons cliniques, préalablement confirmés positifs par PCR en temps réel spécifique de la famille des Chlamydiaceae, ont été de nouveau testés via six PCR en temps réel espèces-spécifiques. Ainsi, il en résulte que Chlamydophila (Cp.) abortus est fréquemment retrouvé chez les bovins, moutons, chevaux, chèvres et cochons. La détection de Cp. psittaci and Chlamydia (C.) suis chez les bovidés (11% et 9% respectivement) ainsi que de Cp. psittaci dans un échantillon dóovidé se révèle quelque peu inattendu. Sur 440 échantillons testés, 56 (soit 12,7%) contiennent l'ADN de deux espèces différentes de chlamydiae. Cp. felis représente l'espèce dominante chez le chat alors que Cp. caviae est présente uniquement chez les cobaye et lapin. Curieusement, ces deux pathogènes sont également détectés dans des échantillons d'origine canine. Les données tendent à démontrer que les infections simultanées par différentes espèces chlamydiales sont rarement des cas isolés et suggèrent dès lors d'étendre la gamme d'hôtes des espèces individuelles. © 2009 Elsevier Ltd.

Richter A.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Sting R.,Chemisches und Veterinaruntersuchungsamt Stuttgart | Popp C.,Tierseuchenkasse Baden Wurttemberg | Rau J.,Chemisches und Veterinaruntersuchungsamt Stuttgart | And 4 more authors.
Epidemiology and Infection | Year: 2012

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) have been isolated from a number of livestock species and persons involved in animal production. We investigated the prevalence of LA-MRSA in fattening turkeys and people living on farms that house fattening turkeys. Eighteen (90%) of 20 investigated flocks were positive for MRSA, and on 12 of the farms 22 (373%) of 59 persons sampled were positive for MRSA. People with frequent access to the stables were more likely to be positive for MRSA. In most flocks MRSA that could be assigned to clonal complex (CC) 398 were detected. In five flocks MRSA of spa-type t002 that is not related to CC398 were identified. Moreover, other methicillin-resistant Staphylococcus spp. were detected on 11 farms and in eight people working on the farms. © Copyright Cambridge University Press 2012.

Eisenberg T.,Landesbetrieb Hessisches Landeslabor | Kutzer P.,Landeslabor Berlin Brandenburg | Peters M.,Chemisches und Veterinaruntersuchungsamt Westfalen | Sing A.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit | And 2 more authors.
Emerging Infectious Diseases | Year: 2014

Corynebacterium ulcerans may cause diphtheria in humans and caseous lymphadenitis in animals. We isolated nontoxigenic tox-bearing C. ulcerans from 13 game animals in Germany. Our results indicate a role for game animals as reservoirs for zoonotic C. ulcerans.

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