Schönau am Königssee, Germany
Schönau am Königssee, Germany

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Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Boernsen B.,Chemisches und Veterinaruntersuchungsamt Freiburg | Naumann G.,Institute For Hygiene Und Umwelt Ihu | Koeppel R.,Kantonales Laboratorium Zurich
Journal fur Verbraucherschutz und Lebensmittelsicherheit | Year: 2014

The inter-laboratory (=ring-trial) validation of 4 food allergen quantification methods using real-time PCR is described. Three single real-time PCR methods for the specific detection and quantification of sesame, almond and Brazil nut were used. Additionally, a multiplex real-time PCR method combining the detection of sesame, almond, Brazil nut and lupine was tested in parallel. Matrix based calibrants (rice cookies) spiked (=incurred) with defined amounts of sesame, almond, lupine and Brazil nut were applied for quantitative evaluation. Cookies based upon wheat and rice flour as well as sauce hollandaise powder each incurred with these allergenic ingredients in the range of 10-123 milligram per kilogram were used as ring-trial samples. The lowest spike level of 10 mg/kg could reproducibly be detected by 6 of 7 PCR systems. In quantitative evaluation of the results, reproducibility standard deviations of approximately 50 % and below were obtained. In addition, the effect of the food matrix on allergen quantification was examined. The range of "recoveries" over all matrices and methods was from 43 to 109 %. © 2014 Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL).


Muller-Hohe E.,Chemisches und Veterinaruntersuchungsamt Freiburg | Ballin U.,Institute For Fische Und Fischereierzeugnisse Cuxhaven | Holland B.,Landeslabor Berlin Brandenburg | Wagler M.,Institute For Hygiene Und Umwelt Hamburg | And 2 more authors.
Archiv fur Lebensmittelhygiene | Year: 2014

The ongoing discussion about the infestation of Pacific wild caught salmon species with larvae of nematodes and the nowadays used practice of assessment raised the question if the amount of infestation of a lot can be influenced by measures performed by the food trader. The results of the investigations performed by the working group "Fish and fishery products" of the Food Chemists Society reveal in coincidence with literature data that the ventral fillet of Pacific wild salmon is heavier infestated with larvae of nematodes than the dorsal fillet. This allows specific measures to influence the amount of investation in a lot of Pacific salmon which was as aspected known to be or was shown by screening tests to be heavily infested by nematodes by removing the most infested parts or the whole ventral part of the fillet. © M. & H. Schaper GmbH & Co.


Demmel A.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit | Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Busch U.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit
Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz | Year: 2016

With the implementation of EU regulation 1169/2011 in December 2014, labelling of allergenic ingredients has been extended to non-prepacked foods. The member states of the European Union were authorised to lay down national rules for the labelling of allergenic ingredients in non-prepacked foods. In Germany, this was accomplished through the introduction of the Vorläufige Lebensmittelinformations-Ergänzungsverordnung (VorlLMIEV). Regulation 1169/2011 also changed the way allergenic ingredients are to be labelled on prepacked foods. This article provides an overview over the current regulations regarding the labelling of food allergens on prepacked and non-prepacked foods in the EU and Germany. © 2016 Springer-Verlag Berlin Heidelberg


PubMed | Chemisches und Veterinaruntersuchungsamt Freiburg and Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit
Type: Journal Article | Journal: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz | Year: 2016

With the implementation of EU regulation 1169/2011 in December 2014, labelling of allergenic ingredients has been extended to non-prepacked foods. The member states of the European Union were authorised to lay down national rules for the labelling of allergenic ingredients in non-prepacked foods. In Germany, this was accomplished through the introduction of the Vorlufige Lebensmittelinformations-Ergnzungsverordnung (VorlLMIEV). Regulation 1169/2011 also changed the way allergenic ingredients are to be labelled on prepacked foods.This article provides an overview over the current regulations regarding the labelling of food allergens on prepacked and non-prepacked foods in the EU and Germany.


Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Schillinger M.,Chemisches und Veterinaruntersuchungsamt Freiburg | Hess N.,Institute For Hygiene Und Umwelt
Journal fur Verbraucherschutz und Lebensmittelsicherheit | Year: 2012

Here, we describe the procedure of a DNA extraction method from maize starch including the method's validation by in-house and interlaboratory tests. The amplifiable amount of maize DNA tested by real-time PCR was used as parameter for evaluating our method. The practical (i. e. relative) limit of detection (LOD) was used as key criterion for assessing the suitability of the extraction method with respect to genetically modified organism analysis. In a round-robin test with ten participating laboratories, satisfactory results were achieved with practical LODs in the range of 0. 1 % with three native maize starch materials. In-house tests showed that this protocol-with an additional purification step-can also be applied for extracting DNA from chemically or enzymatically modified starch. © 2012 Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL).


Knodl C.,Chemisches und Veterinaruntersuchungsamt Freiburg | Yilmaz M.,Chemisches und Veterinaruntersuchungsamt Karlsruhe | Lohneis M.,Chemisches und Veterinaruntersuchungsamt Karlsruhe | Noack D.,Chemisches und Veterinaruntersuchungsamt Karlsruhe | Sabrowski A.,Chemisches und Veterinaruntersuchungsamt Karlsruhe
Fleischwirtschaft | Year: 2010

In order to detect Methicillin resistant Staphylococcus aureus (MRSA) in food of animal origin, we established a combined diagnostic routine method, using both cultural methods and Real-Time PCR at the Chemical and Veterinary Investigation Institute (CVUA) Karlsruhe-Germany. During this study, optimal results were achieved by performing a two-step enrichment procedure followed by streaking on chromogen MRSA-Selective-Agar. A total of 272 food samples were analyzed for the presence of MRSA in order to assess the relevance of different foods as a source of MRSA infections for humans. Contamination rates of 16.6% in raw meat and 15.4% in raw sausage were determined. In detail, MRSA were isolated from 14 out of 42 beef samples (33.3%), from 20 out of 77 poultry samples (26.0%), from 8 out of 76 pork samples (10.5%), from 1 out of 26 (3.8%) minced meat-samples and from 2 out of 13 (15.4%) raw sausages.


Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Grohmann L.,Bundesamt fur Verbraucherschutz und Lebensmittelsicherheit
Journal fur Verbraucherschutz und Lebensmittelsicherheit | Year: 2014

Guidelines for the validation of DNA extraction methods especially in the scope of the analysis of genetically modified sequences in food and feed products by real-time polymerase chain reaction (real-time PCR) are described. Performance testing of extracted DNA is primarily done by determination of the quantity and quality of amplifiable DNA in real-time PCR. Assessment of quality of extracted DNA includes determination of DNA concentration (e.g. fluorimetrically) and evaluation of fragmentation status by gel electrophoresis. A detailed procedure for in-house validation as well as subsequent inter-laboratory validation studies of DNA extraction methods is presented. © 2014 Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL).


Siegel M.,Chemisches und Veterinaruntersuchungsamt Freiburg | Schnur K.,Chemisches und Veterinaruntersuchungsamt Freiburg | Boernsen B.,Chemisches und Veterinaruntersuchungsamt Freiburg | Pietsch K.,Chemisches und Veterinaruntersuchungsamt Freiburg | Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg
European Food Research and Technology | Year: 2012

The first interlaboratory validation of two food allergen quantification methods using real-time PCR is described. Methods for the specific detection and quantification of soybean and white mustard in boiled sausages were used. Matrix-based calibrants spiked with defined amounts of soybean and white mustard were applied for quantitative evaluation. The lowest spike level of 10 mg soybean and white mustard per kilogram could reproducibly be detected. Recovery in spiked sausages was between 82 and 99 % for soy and between 80 and 93 % for mustard. Reproducibility standard deviation was in the range that would be acceptable, for example, for quantitative GMO analytical methods (<35 %). © 2012 Springer-Verlag.


Siegel M.,Chemisches und Veterinaruntersuchungsamt Freiburg | Mutschler A.,Chemisches und Veterinaruntersuchungsamt Freiburg | Boernsen B.,Chemisches und Veterinaruntersuchungsamt Freiburg | Pietsch K.,Chemisches und Veterinaruntersuchungsamt Freiburg | Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg
European Food Research and Technology | Year: 2013

For the quantification of food allergens by real-time polymerase chain reaction (PCR), food matrix standards with defined levels of spiked allergenic food ingredients can be used. The production and homogeneity testing of selected materials as sausages, cookies and sauce hollandaise powder is described. Except for egg and milk, all relevant allergenic ingredients were spiked to each material. Allergens were spiked and quantified as food ingredients, for example, peanut or lupine flour, at levels of 5-400 mg/kg. Material with sufficient homogeneity could be produced even at low levels of 5-10 mg of the allergenic ingredient per kilogram. The effect of the food matrix on allergen quantification was checked. The bias caused by this effect was in an acceptable range for the tested materials. The materials produced within this study were used as samples and for calibration in inter-laboratory validation studies for the quantification of allergenic food ingredients by real-time PCR. The results of this study are a contribution how to produce such reference materials for allergen analysis in the near future. Before threshold or action values of allergens in food are set, the availability of reference materials is essential. © 2013 Springer-Verlag Berlin Heidelberg.


Muller-Hohe E.,Chemisches und Veterinaruntersuchungsamt Freiburg | Ballin U.,Institute For Fische Und Fischereierzeugnisse Cuxhaven | Holland B.,Landeslabor Berlin Brandenburg | Wagler M.,Institute For Hygiene Und Umwelt Hamburg | And 2 more authors.
Archiv fur Lebensmittelhygiene | Year: 2014

The ongoing discussion about the infestation of Pacific wild caught salmon species with larvae of nematodes and the nowadays used practice of assessment raised the question if the amount of infestation of a lot can be influenced by measures performed by the food trader. The results of the investigations performed by the working group "Fish and fishery products" of the Food Chemists Society reveal in coincidence with literature data that the ventral fillet of Pacific wild salmon is heavier infestated with larvae of nematodes than the dorsal fillet. This allows specific measures to influence the amount of investation in a lot of Pacific salmon which was as aspected known to be or was shown by screening tests to be heavily infested by nematodes by removing the most infested parts or the whole ventral part of the fillet. © M. & H. Schaper GmbH & Co.

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