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Knodl C.,Chemisches und Veterinaruntersuchungsamt Freiburg | Yilmaz M.,Chemisches und Veterinaruntersuchungsamt Karlsruhe | Lohneis M.,Chemisches und Veterinaruntersuchungsamt Karlsruhe | Noack D.,Chemisches und Veterinaruntersuchungsamt Karlsruhe | Sabrowski A.,Chemisches und Veterinaruntersuchungsamt Karlsruhe
Fleischwirtschaft | Year: 2010

In order to detect Methicillin resistant Staphylococcus aureus (MRSA) in food of animal origin, we established a combined diagnostic routine method, using both cultural methods and Real-Time PCR at the Chemical and Veterinary Investigation Institute (CVUA) Karlsruhe-Germany. During this study, optimal results were achieved by performing a two-step enrichment procedure followed by streaking on chromogen MRSA-Selective-Agar. A total of 272 food samples were analyzed for the presence of MRSA in order to assess the relevance of different foods as a source of MRSA infections for humans. Contamination rates of 16.6% in raw meat and 15.4% in raw sausage were determined. In detail, MRSA were isolated from 14 out of 42 beef samples (33.3%), from 20 out of 77 poultry samples (26.0%), from 8 out of 76 pork samples (10.5%), from 1 out of 26 (3.8%) minced meat-samples and from 2 out of 13 (15.4%) raw sausages. Source


Demmel A.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit | Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Busch U.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit
Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz | Year: 2016

With the implementation of EU regulation 1169/2011 in December 2014, labelling of allergenic ingredients has been extended to non-prepacked foods. The member states of the European Union were authorised to lay down national rules for the labelling of allergenic ingredients in non-prepacked foods. In Germany, this was accomplished through the introduction of the Vorläufige Lebensmittelinformations-Ergänzungsverordnung (VorlLMIEV). Regulation 1169/2011 also changed the way allergenic ingredients are to be labelled on prepacked foods. This article provides an overview over the current regulations regarding the labelling of food allergens on prepacked and non-prepacked foods in the EU and Germany. © 2016 Springer-Verlag Berlin Heidelberg Source


Demmel A.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit | Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Busch U.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit
Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz | Year: 2016

With the implementation of EU regulation 1169/2011 in December 2014, labelling of allergenic ingredients has been extended to non-prepacked foods. The member states of the European Union were authorised to lay down national rules for the labelling of allergenic ingredients in non-prepacked foods. In Germany, this was accomplished through the introduction of the Vorläufige Lebensmittelinformations-Ergänzungsverordnung (VorlLMIEV). Regulation 1169/2011 also changed the way allergenic ingredients are to be labelled on prepacked foods. This article provides an overview over the current regulations regarding the labelling of food allergens on prepacked and non-prepacked foods in the EU and Germany. © 2016, Springer-Verlag Berlin Heidelberg. Source


Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Grohmann L.,Bundesamt fur Verbraucherschutz und Lebensmittelsicherheit
Journal fur Verbraucherschutz und Lebensmittelsicherheit | Year: 2014

Guidelines for the validation of DNA extraction methods especially in the scope of the analysis of genetically modified sequences in food and feed products by real-time polymerase chain reaction (real-time PCR) are described. Performance testing of extracted DNA is primarily done by determination of the quantity and quality of amplifiable DNA in real-time PCR. Assessment of quality of extracted DNA includes determination of DNA concentration (e.g. fluorimetrically) and evaluation of fragmentation status by gel electrophoresis. A detailed procedure for in-house validation as well as subsequent inter-laboratory validation studies of DNA extraction methods is presented. © 2014 Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL). Source


Waiblinger H.-U.,Chemisches und Veterinaruntersuchungsamt Freiburg | Boernsen B.,Chemisches und Veterinaruntersuchungsamt Freiburg | Naumann G.,Institute For Hygiene Und Umwelt Ihu | Koeppel R.,Kantonales Laboratorium Zurich
Journal fur Verbraucherschutz und Lebensmittelsicherheit | Year: 2014

The inter-laboratory (=ring-trial) validation of 4 food allergen quantification methods using real-time PCR is described. Three single real-time PCR methods for the specific detection and quantification of sesame, almond and Brazil nut were used. Additionally, a multiplex real-time PCR method combining the detection of sesame, almond, Brazil nut and lupine was tested in parallel. Matrix based calibrants (rice cookies) spiked (=incurred) with defined amounts of sesame, almond, lupine and Brazil nut were applied for quantitative evaluation. Cookies based upon wheat and rice flour as well as sauce hollandaise powder each incurred with these allergenic ingredients in the range of 10-123 milligram per kilogram were used as ring-trial samples. The lowest spike level of 10 mg/kg could reproducibly be detected by 6 of 7 PCR systems. In quantitative evaluation of the results, reproducibility standard deviations of approximately 50 % and below were obtained. In addition, the effect of the food matrix on allergen quantification was examined. The range of "recoveries" over all matrices and methods was from 43 to 109 %. © 2014 Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL). Source

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