Chemisches und Veterinaruntersuchungsamt CVUA

Karlsruhe, Germany

Chemisches und Veterinaruntersuchungsamt CVUA

Karlsruhe, Germany
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Buchholz S.,Swiss Bee Research Center | Merkel K.,University of Bremen | Spiewok S.,Martin Luther University of Halle Wittenberg | Imdorf A.,Swiss Bee Research Center | And 7 more authors.
Apidologie | Year: 2011

To explore alternative strategies to synthetic insecticides for control of Aethina tumida, the small hive beetle (SHB), treatments already established against two other honeybee pests, Varroa destructor and Galleria mellonella, were investigated. In the laboratory, eggs, larvae, and adults of SHB were treated with thymol (10, 20, and 50 mg) or with organic acids: 85% formic acid (0. 125, 0. 25, 0. 5, 0. 75, 1.0, and 2.0 mL), 15% lactic acid (0. 5, 1. 0, 1.5, and 2.0 mL), oxalic acid (dihydrate crystals 35 g/L; 0. 25, 0. 5, 0. 75, 1.0, and 2.0 mL), and 65% acetic acid (0. 5, 1. 0, 1.5, and 2.0 mL). Some of the chosen concentrations of formic and oxalic acid resulted in high mortalities of all SHB life stages. Therefore, they were further evaluated in the field utilising standard methods for control of V. destructor in Europe. After exposure to evaporating formic acid (85%, Nassenheider®) and oxalic acid (2 g dehydrate crystals, Varrox®), mortality in all SHB tested stages did not increase significantly. The same was true for trials with 85% (adults) or 60% (eggs and larvae) formic acid, evaporating from sponge tissues in diagnostic trays. In fact, some SHBs used the diagnostic trays to hide or oviposit. Despite treating extracted honey combs with 65% acetic acid, SHBs still reproduced on the combs' pollen cells. In conclusion, none of the tested methods can be recommended to control SHBs. © INRA, DIB-AGIB and Springer Science+Business Media B.V., 2011.

Hertzsch R.,University of Leipzig | Emmerich I.U.,University of Leipzig | Lachenmeier D.W.,Chemisches und Veterinaruntersuchungsamt CVUA | Sproll C.,Chemisches und Veterinaruntersuchungsamt CVUA | And 4 more authors.
Tierarztliche Praxis Ausgabe G: Grosstiere - Nutztiere | Year: 2015

Opioid alkaloids were identified in the urine of horses during an anti-doping control and in a case of intoxication. In both cases, it was suspected that the horses had ingested poppy-contaminated feed. To verify this suspicion, possible opioid alkaloid sources in Germany were identified through a literature research. Additionally, the contaminated feed was botanically and chemically analysed. The results indicated that both cases were most probably caused by the poppy in the feed. This highlights the previously underestimated risk of an intake of poppy-contaminated feed in horses. Recommendations are formulated for the prevention of positive doping-test results and intoxications by poppy-contaminated feeds in horses. Furthermore, a threshold for morphine in urine samples in competing horses is proposed. © Schattauer 2015.

Lachenmeier D.W.,Chemisches und Veterinaruntersuchungsamt CVUA | Lachenmeier D.W.,TU Dresden | Kanteres F.,Independent researcher | Rehm J.,TU Dresden | And 2 more authors.
Alcoholism: Clinical and Experimental Research | Year: 2014

Background: Given the association between alcohol consumption and negative health consequences, there is a need for individuals to be aware of their consumption of ethanol, which requires knowledge of serving sizes and alcoholic strength. This study is one of the first to systematically investigate the ability to discriminate alcoholic strength by taste. Methods: Nine discrimination tests (total n = 413) according to International Standardization Organization (ISO) 4120 sensory analysis methodology "triangle test" were performed. Results: A perceptible difference was found for vodka in orange juice (0.0 vs. 0.5% vol; 0 vs. 1% vol), pilsner and wheat beer (0.5 vs. 5% vol), and vodka in orange juice (5 vs. 10% vol, 20 vs. 30% vol, and 30 vs. 40% vol). The percentage of the population perceiving a difference between the beverages varied between 36 and 73%. Alcoholic strength (higher vs. lower) was correctly assigned in only 4 of the 7 trials at a significant level, with 30 to 66% of the trial groups assigning the correct strength. For the trials that included beverages above 40% vol (vodka unmixed, 40 vs. 50% vol and vodka in orange juice, 40 vs. 50% vol), testers could neither perceive a difference between the samples nor assign correct alcoholic strength. Conclusions: Discrimination of alcoholic strength by taste was possible to a limited degree in a window of intermediate alcoholic strengths, but not at higher concentrations. This result is especially relevant for drinkers of unlabeled, over-proof unrecorded alcoholic beverages who would potentially ingest more alcohol than if they were to ingest commercial alcohol. Our study provides strong evidence for the strict implementation and enforcement of labeling requirements for all alcoholic beverages to allow informed decision making by consumers. © 2014 by the Research Society on Alcoholism.

Anderson A.,Chemisches und Veterinaruntersuchungsamt CVUA | Pietsch K.,Chemisches und Veterinaruntersuchungsamt CVUA | Zucker R.,Bavarian Health and Food Safety Authority | Mayr A.,Bavarian Health and Food Safety Authority | And 5 more authors.
Food Analytical Methods | Year: 2011

A 5′ nuclease duplex real-time polymerase chain reaction (PCR) assay was developed and validated with various food products for the specific and fast detection of Salmonella spp. in food. The assay used previously published primers in combination with a newly developed probe targeting the invA gene. An internal amplification control, which is coamplified in a duplex PCR, was included in the assay. The analysis of 1,934 natural food samples with real-time PCR and the cultural method in parallel resulted in a relative accuracy of 100% and 99. 84% respectively, depending on the enrichment procedure in which buffered peptone water and selective enrichment in Rappaport-Vassiliadis (RV) broth were employed. The duplex real-time PCR assay has proven to be a specific, sensitive and fast screening method for Salmonella spp. in food. The overall analysis time of the PCR method was approximately 28 h, in contrast to 4 to 5 days with conventional Salmonella diagnostics. The developed assay has been shown to be a reliable diagnostic tool for use in routine analysis. It has been validated thoroughly and has become an official method in Germany for the detection of Salmonella spp. in food. © 2010 Springer Science+Business Media, LLC.

Monakhova Y.B.,Chemisches und Veterinaruntersuchungsamt CVUA | Monakhova Y.B.,Bruker Biospin Gmbh | Monakhova Y.B.,Chernyshevsky Saratov State University | Ruge I.,Chemisches und Veterinaruntersuchungsamt CVUA | And 3 more authors.
International Journal for Vitamin and Nutrition Research | Year: 2013

A methodology utilizing 1 H NMR spectroscopy has been developed to measure the concentration of coenzyme Q10 (CoQ10) in dietary supplements. For sample preparation, a very simple dilution with deuterated chloroform and addition of internal standard is sufficient. CoQ10 produces a distinct peak of the CH groups in the isoprene side chain of the molecule in the d 5.15-5.05 ppm range, where it can be distinguished from other matrix compounds. The method was shown to be of adequate sensitivity with a limit of detection (LOD) of 7.8 mg/L, to control the CoQ10 content in the majority of the products. The precision expressed as relative standard deviation was around 5 %; linearity was observed from 14 to 2000 mg/L (R = 0.99). The developed methodology was applied for the analysis of 21 food supplements (capsules, tablets, and liquid products). On the basis of the labeled amounts, only two products contained substantially lower concentrations of CoQ10 (57 % and 51 %). All other concentrations varied between 83 % and 190 % with respect to labeling. The developed NMR method may be used by quality assurance laboratories for routine control of CoQ10 products. © 2013 Hans Huber Publishers, Hogrefe AG, Bern.

Lachenmeier D.W.,Chemisches und Veterinaruntersuchungsamt CVUA | Uebelacker M.,Chemisches und Veterinaruntersuchungsamt CVUA
Journal of Automated Methods and Management in Chemistry | Year: 2011

Acetaldehyde (ethanal) is a genotoxic carcinogen, which may occur naturally or as an added flavour in foods. We have developed an efficient method to analyze the compound in a wide variety of food matrices. The analysis is conducted using headspace (HS) gas chromatography (GC) with flame ionization detector. Using a robot autosampler, the samples are digested in full automation with simulated gastric fluid (1h at 37()C) under shaking, which frees acetaldehyde loosely bound to matrix compounds. Afterwards, an aliquot of the HS is injected into the GC system. Standard addition was applied for quantification to compensate for matrix effects. The precision of the method was sufficient (<3% coefficient of variation). The limit of detection was 0.01mg/L and the limit of quantification was 0.04mg/L. 140 authentic samples were analyzed. The acetaldehyde content in apples was 0.97 ± 0.80 mg/kg, orange juice contained 3.86 ± 2.88 mg/kg. The highest concentration was determined in a yoghurt (17mg/kg). A first-exposure estimation resulted in a daily acetaldehyde intake of less than 0.1mg/kg bodyweight from food, which is considerably lower than the exposures from alcohol consumption or tobacco smoking. Copyright © 2011 Michael Uebelacker and Dirk W. Lachenmeier.

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