Bogdanov D.V.,Chelyabinsk State Medical Academy
Kardiologiya | Year: 2012
We examined 86 patients with various forms and variants of hypertrophic cardiomyopathy (HCM). Examination of 69 patients with nonobstructive form of the disease included echocardiography. The state of left atrium (LA) was studied by measurement of its systolic and diastolic longitudinal and transversal dimensions with calculation of main parameters of contractility. In 60% of patients with HCM including those with nonobstuctive form we found spherical remodeling of LA with lowering of its contractility. Coefficient of remodeling was suggested as ratio of transversal and longitudinal dimensions of its cavity. In control group mean value of this parameter was equal to 0.38±0.G1 relative units, in the group of patients with nonobstructive HCM - 0.61 ±0.02 units (p<0.05).
Osikov M.V.,Chelyabinsk State Medical Academy |
Grigoriev T.A.,Chelyabinsk State Medical Academy
Bulletin of Experimental Biology and Medicine | Year: 2012
Activity of plasma proteolytic systems (fibrin formation, fibrinolysis, and anticoagulant system) and the possibility for correction of changes in these systems with erythropoietin were studied in experiments on outbred albino rats with experimental renal failure. Renal failure was induced by a single subcutaneous injection of mercury chloride (II). The parameters were estimated on day 5 postinjection. Erythropoietin in a single dose of 5000 U/kg was administered on day 4. Renal failure was accompanied by activation of fibrin formation (with factors of the common and intrinsic pathways of blood coagulation), increase in antithrombin activity, and inhibition of the fibrinolytic system. Treatment with erythropoietin led to partial recovery of fibrin formation and fibrinolysis. Under analytical in vitro conditions, 30-min incubation of whole blood from healthy donors with erythropoietin in doses of 1.9-30.0 U/liter was followed by a dose-dependent inhibition of the fibrin formation system and activation of fibrinolysis. © 2012 Springer Science+Business Media, Inc.
Privalov V.A.,Chelyabinsk State Medical Academy |
Lappa A.V.,Chelyabinsk State University |
Bigbov E.N.,Chelyabinsk State Medical Academy
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2011
A photodynamic therapy experiment on 118 inbred white mice with transplanted Ehrlich's tumor (mouse mammary gland adenocarcinoma) is performed to reveal mechanisms of necrosis formation. In 7-10 days the tumor of 1-1.5 cm diameter is formed under skin at the injection point, and PDT procedure is applied. There were used a chlorine type photosensitizer Radachlorine™ and 662 nm wavelength diode laser. The drug is injected by intravenously at the dose of 40 mg/kg; the irradiation is executed in 2-2.5 hours at the surface dose of about 200 J/cm2. Each of the mice had a photochemical reaction in form of destructive changes at the irradiation region with subsequent development of dry coagulation necrosis. After rejection of the necrosis there occurred epithelization of defect tissues in a tumor place. Histological investigations were conducted in different follow-up periods, in 5 and 30 min, 1, 3, 6, and 12 hours, 1, 3, 7 and 28 days after irradiation. They included optical microscopy, immune marker analysis, morphometry with measurements of volume density of epithelium, tumor stroma and necroses, vascular bed. The investigations showed that an important role in damaging mechanisms of photodynamic action belongs to hypoxic injuries of tumor mediated by micro vascular disorders and blood circulatory disturbances. The injuries are formed in a few stages: microcirculation angiospasm causing vessel paresis, irreversible stases in capillaries, diapedetic hemorrhages, thromboses, and thrombovasculitis. It is marked mucoid swelling and fibrinoid necrosis of vascular tissue. Progressive vasculitises result in total vessel obliteration and tumor necrosis. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).
Sushko I.,EADMET GmbH |
Salmina E.,Russian Academy of Sciences |
Potemkin V.A.,Chelyabinsk State Medical Academy |
Poda G.,Ontario Cancer Institute |
And 2 more authors.
Journal of Chemical Information and Modeling | Year: 2012
The article presents a Web-based platform for collecting and storing toxicological structural alerts from literature and for virtual screening of chemical libraries to flag potentially toxic chemicals and compounds that can cause adverse side effects. An alert is uniquely identified by a SMARTS template, a toxicological endpoint, and a publication where the alert was described. Additionally, the system allows storing complementary information such as name, comments, and mechanism of action, as well as other data. Most importantly, the platform can be easily used for fast virtual screening of large chemical datasets, focused libraries, or newly designed compounds against the toxicological alerts, providing a detailed profile of the chemicals grouped by structural alerts and endpoints. Such a facility can be used for decision making regarding whether a compound should be tested experimentally, validated with available QSAR models, or eliminated from consideration altogether. The alert-based screening can also be helpful for an easier interpretation of more complex QSAR models. The system is publicly accessible and tightly integrated with the Online Chemical Modeling Environment (OCHEM, http://ochem.eu). The system is open and expandable: any registered OCHEM user can introduce new alerts, browse, edit alerts introduced by other users, and virtually screen his/her data sets against all or selected alerts. The user sets being passed through the structural alerts can be used at OCHEM for other typical tasks: exporting in a wide variety of formats, development of QSAR models, additional filtering by other criteria, etc. The database already contains almost 600 structural alerts for such endpoints as mutagenicity, carcinogenicity, skin sensitization, compounds that undergo metabolic activation, and compounds that form reactive metabolites and, thus, can cause adverse reactions. The ToxAlerts platform is accessible on the Web at http://ochem.eu/alerts, and it is constantly growing. © 2012 American Chemical Society.
Manoylov A.E.,Chelyabinsk State Medical Academy
Cardiovascular Therapy and Prevention (Russian Federation) | Year: 2012
Aim. To study the physicians' knowledge of the high blood pressure (BP) risk and to assess their attitude towards the prevention of arterial hypertension (AH). Material and methods. In total, 118 internal disease specialists from three Russian regions participated in an anonymous questionnaire survey on the knowledge of high BP risk and the attitude towards the prevention of AH and its complications. The mean age of participants ( 13 men and 105 women) was 46,53± 11,64 years. The respondents' answers were compared to the information in the current clinical AH guidelines. Results. More than one-half of the respondents demonstrated inadequate professional knowledge on prediction and prevention of AH complications. These physicians use the "traditional" disease model and do not consider elevated BP as a risk factor; predict the risk of AH complications using subjective complaints of their patients; do not realise the importance of the target organ damage (TOD) prevention; and limit their preventive strategy only to the prevention of hypertensive crises. Conclusion. The demonstrated attitude towards AH management could be a serious barrier for effective AH prevention. This group of physicians needs extensive education and training, in order to modify their attitude and to increase their commitment to preventive strategies.
Garbuzenko D.V.,Chelyabinsk State Medical Academy
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2013
The data of the literature on the mechanisms of restructuring of vascular bed in response to hemodynamic changes due to portal hypertension. Despite the fact that these changes are compensatory-adaptive reaction to the deteriorating conditions of blood circulation, they contribute to its progression, promoting the development of serious complications, one of which was bleeding from esophageal varices.
Manoylov A.E.,Chelyabinsk State Medical Academy
Kardiologiya | Year: 2012
We studied awareness of physicians of the city health clinics (polyclinics) about the causes of heart failure in hypertensive patients and their attitude to the prevention of complications of hypertension. The study involved 194 general practitioners from five regions of Russia. The questionnaire method in the form of single-blind questionnaire survey was used. It was found that 36.6% (95% confidence interval [Di] from 29.81to 43.80) of respondents were aware of the cause-effect relation between hypertension and heart failure. Only 31.44% (95% DI from 24.98 to 38.48) medical professionals perceived left ventricular hypertrophy as risk factor for heart failure in people with high blood pressure. The percentage of internists-respondents who were aware of the major mechanisms of heart failure in hypertension was 28.37% (95% DI from 21.18 to 4.16]. Outdated or incorrect professional notions can become a barrier to early diagnosis, treatment and prevention of heart failure in patients with hypertension.
Bryukhin G.V.,Chelyabinsk State Medical Academy |
Sizonenko M.L.,Chelyabinsk State Medical Academy
Bulletin of Experimental Biology and Medicine | Year: 2013
We compared the parameters of physiological maturity in the offspring of albino Wistar rats with experimental chronic liver disease of various genesis (toxic, autoimmune, and alcoholic). It was found that chronic liver disease in female rats can be a cause of physiological immaturity of the offspring (increased stillbirth rate, reduced viability, and delayed disappearance of immaturity). © 2013 Springer Science+Business Media New York.
Osikov M.V.,Chelyabinsk State Medical Academy
Bulletin of Experimental Biology and Medicine | Year: 2014
A short-term open prospective study examined 62 patients at the terminal stage of chronic renal failure. The experimental group received erythropoietin in a total dose of about 40,000 U. The expression of glycoproteins IIb-IIIa, IIb, and Ib was enhanced, the content of LPO products was elevated, and SOD and catalase activities were reduced in platelets from patients with chronic renal failure. Administration of erythropoietin partially restored free radical oxidation and expression of glycoproteins IIb-IIIa, IIb, and Ib in platelets. A significant correlation was revealed between the expression of platelet receptors on the one hand, and content of LPO products and SOD and catalase activities, on the other hand. © 2014 Springer Science+Business Media.
Zorina I.G.,Chelyabinsk State Medical Academy
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2012
Environmentalactor is one of the most accurate indicators of determining the health of the population, especially in childhood and adolescence. A significant increase in the amount of data on the status of school health and the environment are the most precise and correct, in view of causation, to evaluate an individual's health at prenosological level in the presence of a specialized database of automated systems. The aim of our study the study of fundamental interactions and patterns of environmental factors in general and neuropsychiatrie disease in schoolchildren of the city of Chelyabinsk. Revealed that among the multi-pollutant air Chelyabinsk first and second rank positions are occupied by selectively harmful to the central nervous system (lead, arsenic, manganese), or have complex adverse effects on the central nervous system, combined with damaging effects on the respiratory and cardio- vascular, urogenital system and blood forming organs, involving the immune mechanism (chromium compounds, lead, arsenic, carbon monoxide and manganese).