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Venkateswara Rao P.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Yugandhar N.M.,Andhra University
International Journal of Pharmacy and Technology | Year: 2011

The aim of present study was to produce pectinase enzyme using Ficus religiosa leaves as a substrate and the microorganism is Aspergillus niger - NCIM 548 in a solid state fermentation process. In the process the microorganism is cultivated on a solid substrate enriched with a high concentration of nutrients, micronutrients and materials and having large surface area. Process variables such as size of inoculum, pH, temperature, particle size and moisture content were optimized to achieve the maximum production of pectinases. The increased level of pectinase production was recorded at pH 5.0 and temperature 30°C in solid-state conditions. The optimum inoculum size was 1×105 ml-1, five hundred micrometer particle size and 70% moisture content of the substrate were optimum for the maximum production of pectinases in solid-state condition. Higher titres pectinases were observed when medium was supplemented with carbon (4% glucose) and nitrogen (ammonium sulphate, 0.3%) sources. Under optimum conditions, maximum production pectinase was 34.12 U/ml in solid state fermentation.

Sridhar S.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Dinda S.C.,Berhampur University | Prasad Y.R.,Andhra University
E-Journal of Chemistry | Year: 2011

A series of new chalcones (3a-g) were prepared by Claisen-Schmidt condensation of 3-acetyl-2,5-dimethylfuran with various substituted aromatic aldehydes in presence of aqueous solution of potassium hydroxide and ethanol at room temperature. The synthesized chalcones were characterized by means of their IR, 1H NMR spectral data and elemental analyses. When these chalcones were evaluated for antimicrobial and anti-inflammatory activities, some of them were found to possess significant activity, when compared to standard drugs.

Vidyadhara S.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Sasidhar R.L.,Chebrolu Hanumaiah Institute of Pharmaceutical science
Tropical Journal of Pharmaceutical Research | Year: 2015

Purpose: To prepare and characterize tablets loaded with self-nanoemulsifying drug delivery system (SNEDDS) containing docetaxel (DTL). Method: SNEDDS of docetaxel were prepared using various oils, surfactants, co-surfactant and solvents to improve the dissolution rate and bioavailability of the poorly water-soluble chemotherapeutic agent. The SNEDDS components were preliminarily screened for the solubility of the drug in various vehicles, miscibility of excipients, rate of emulsification and ternary phase diagrams. The tablets were prepared by direct compression process with a porous carrier, magnesium alumino-metasilicate, and subsequently loaded with SNEDDS by a simple absorption method. The tablets were then characterized for physical parameters, including tablet hardness, weight variation, disintegration, drug content and invitro drug release. Results: Cremophor-EL, polysorbate-80 and dehydrated alcohol mixture in the ratio 85:10:5 yielded docetaxel SNEDDS with droplet size of 12.16 nm and polydispersity (PDI) of 0.039. Tablets with high porosity suitable for loading with SNEDDS and containg the super-disintegrants, crosscarmellose sodium and sodium starch glycolate, in a concentration of 3, 4 and 5 %, achieved complete dissolution of docetaxel from the tablets. In vitro release of docetaxel from SNEDDS and the tablets was similar (p < 0.05). Conclusion: SNEDDS of docetaxel is a promising approach to achieving a solid dosage form of the liquid-loaded drug delivery systems for enhancing the solubility and dissolution rate of the drug, and hence also its bioavailability. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.

Nadella T.R.,Alkem Research Center | Suryadevara V.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Lankapalli S.R.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Mandava V.B.R.,Krishna University | Bandarupalli D.,Vignan Pharmacy College
Journal of Pharmaceutical and Biomedical Analysis | Year: 2016

A simple, rapid, specific and precise liquid chromatography-tandem mass spectrophotometric (LC-MS/MS) method was developed and validated for quantification of busulfan, in human plasma. busulfan d8 was used as internal standard, added to plasma sample prior to extraction using acetonitrile as a precipitating agent. Chromatographic separation was achieved on phenomenex kinetex C18 column (50mm×2.1mm, 2.6μm) with acteonitrile: 10 mM ammonium formate buffer (80:20v/v) as an isocratic mobile phase with a flow rate of 0.5mLmin-1. Quantitation was performed by transition of 264.1→151.1 (m/z) for busulfan and 272.1→159.1 (m/z) for busulfan d8. The lower limit of quantitation was 0.2 ng mL-1 with a 100μL plasma sample. The concentrations of nine working standards showed linearity between 0.2 and 100 ng mL-1 (r2≥0.9986). Chromatographic separation was achieved within 2.0min. The average extraction recoveries of 3quality control concentrations were 92.52% for busulfan and 90.75% for busulfan d8. The coefficient of variation was ≤15% for intra- and inter-batch assays. The developed method was successfully applied for the determination of Busulfan pharmacokinetics after oral administration. © 2015 Elsevier B.V.

Suryadevaravidyadhara,Chebrolu Hanumaiah Institute of Pharmaceutical science | Rao Y.S.,Vignan Institute of Pharmaceutical Sciences | Ramu A.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Lankapalli Sasidhar R.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Ramya A.J.,Chebrolu Hanumaiah Institute of Pharmaceutical science
Oriental Journal of Chemistry | Year: 2013

A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method hss been developed snd validated for the estimstion of cinitspride snd pantoprazole simultaneously in combined dosage form. The two components cinitapride and pantoprazole were well resolved on an isocratic 018 column, utilizing a mobile phase composition of acetonitrile: phosphate buffer (50:50, v/v, pH 6.8) at a flow rate of 1.0 mL/min with UV detection at 281 nm. The retention time of cinitapride and pantoprazole were 4.5 mm and 5.4mm respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOG) and robustness as per ICH guidelines. Linearity for cinitspride and pantoprazole were found in the range of 1.5-10.Spg/ml and 20-l4Opg/ml, respectively. The percentage recoveries for cinitapride and pantoprazole ranged from 97.9-103.44 % and 98.9- 103.1%, respectively The proposed method could be used for routine analysis of cinitapride and pantoprazole in their combined doasge forms.

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