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Rambabu R.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Vidyadhara S.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Subbarao J.,Chebrolu Hanumaiah Institute of Pharmaceutical science
Indian Drugs | Year: 2014

A simple and sensitive spectrophotometric method for the determination of ramipril and telmisartan in pharmaceutical dosage forms has been developed. The absorption maxima were found at 220nm for ramipril and 297nm for telmisartan using 0.1N NaOH as solvent. Beer's law was obeyed for both the drugs in the concentration range of 2-10μg/ml with correlation coefficients 0.999 for both ramipril and telmisartan. The limits of detection for ramipril and telmisartan were found to be 0.142 and 0.405μg/mL respectively and the limits of quantitation were 0.43 and 1.22μg/mL. Accuracy of the method was verified by performing recovery studies using simultaneous equation method and found to be 98.33 to 99.54%w/w for ramipril and 99.36 to 99.82 %w/w for telmisartan. %RSD of repeatability and intermediate precision studies were found to be <2 for both the drugs. Ruggedness of the method was checked by changing analyst and instrument used. In both the cases, the %RSD was found to be less than 2. © Copyright 2013 by Indian Drugs Online. All Rights Reserved.


Venkateswara Rao P.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Yugandhar N.M.,Andhra University
International Journal of Pharmacy and Technology | Year: 2011

The aim of present study was to produce pectinase enzyme using Ficus religiosa leaves as a substrate and the microorganism is Aspergillus niger - NCIM 548 in a solid state fermentation process. In the process the microorganism is cultivated on a solid substrate enriched with a high concentration of nutrients, micronutrients and materials and having large surface area. Process variables such as size of inoculum, pH, temperature, particle size and moisture content were optimized to achieve the maximum production of pectinases. The increased level of pectinase production was recorded at pH 5.0 and temperature 30°C in solid-state conditions. The optimum inoculum size was 1×105 ml-1, five hundred micrometer particle size and 70% moisture content of the substrate were optimum for the maximum production of pectinases in solid-state condition. Higher titres pectinases were observed when medium was supplemented with carbon (4% glucose) and nitrogen (ammonium sulphate, 0.3%) sources. Under optimum conditions, maximum production pectinase was 34.12 U/ml in solid state fermentation.


Vidyadhara S.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Sasidhar R.L.,Chebrolu Hanumaiah Institute of Pharmaceutical science
Tropical Journal of Pharmaceutical Research | Year: 2015

Purpose: To prepare and characterize tablets loaded with self-nanoemulsifying drug delivery system (SNEDDS) containing docetaxel (DTL). Method: SNEDDS of docetaxel were prepared using various oils, surfactants, co-surfactant and solvents to improve the dissolution rate and bioavailability of the poorly water-soluble chemotherapeutic agent. The SNEDDS components were preliminarily screened for the solubility of the drug in various vehicles, miscibility of excipients, rate of emulsification and ternary phase diagrams. The tablets were prepared by direct compression process with a porous carrier, magnesium alumino-metasilicate, and subsequently loaded with SNEDDS by a simple absorption method. The tablets were then characterized for physical parameters, including tablet hardness, weight variation, disintegration, drug content and invitro drug release. Results: Cremophor-EL, polysorbate-80 and dehydrated alcohol mixture in the ratio 85:10:5 yielded docetaxel SNEDDS with droplet size of 12.16 nm and polydispersity (PDI) of 0.039. Tablets with high porosity suitable for loading with SNEDDS and containg the super-disintegrants, crosscarmellose sodium and sodium starch glycolate, in a concentration of 3, 4 and 5 %, achieved complete dissolution of docetaxel from the tablets. In vitro release of docetaxel from SNEDDS and the tablets was similar (p < 0.05). Conclusion: SNEDDS of docetaxel is a promising approach to achieving a solid dosage form of the liquid-loaded drug delivery systems for enhancing the solubility and dissolution rate of the drug, and hence also its bioavailability. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.


Suryadevaravidyadhara,Chebrolu Hanumaiah Institute of Pharmaceutical science | Rao Y.S.,Vignan Institute of Pharmaceutical Sciences | Ramu A.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Lankapalli Sasidhar R.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Ramya A.J.,Chebrolu Hanumaiah Institute of Pharmaceutical science
Oriental Journal of Chemistry | Year: 2013

A new rapid, precise and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method hss been developed snd validated for the estimstion of cinitspride snd pantoprazole simultaneously in combined dosage form. The two components cinitapride and pantoprazole were well resolved on an isocratic 018 column, utilizing a mobile phase composition of acetonitrile: phosphate buffer (50:50, v/v, pH 6.8) at a flow rate of 1.0 mL/min with UV detection at 281 nm. The retention time of cinitapride and pantoprazole were 4.5 mm and 5.4mm respectively. The developed method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOG) and robustness as per ICH guidelines. Linearity for cinitspride and pantoprazole were found in the range of 1.5-10.Spg/ml and 20-l4Opg/ml, respectively. The percentage recoveries for cinitapride and pantoprazole ranged from 97.9-103.44 % and 98.9- 103.1%, respectively The proposed method could be used for routine analysis of cinitapride and pantoprazole in their combined doasge forms.


Sridhar S.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Dinda S.C.,Berhampur University | Prasad Y.R.,Andhra University
E-Journal of Chemistry | Year: 2011

A series of new chalcones (3a-g) were prepared by Claisen-Schmidt condensation of 3-acetyl-2,5-dimethylfuran with various substituted aromatic aldehydes in presence of aqueous solution of potassium hydroxide and ethanol at room temperature. The synthesized chalcones were characterized by means of their IR, 1H NMR spectral data and elemental analyses. When these chalcones were evaluated for antimicrobial and anti-inflammatory activities, some of them were found to possess significant activity, when compared to standard drugs.


Subbarao J.,Chebrolu hanumaiah institute of pharmaceutical science | Venkateswara Rao P.,Chebrolu hanumaiah institute of pharmaceutical science | Vidyadhara S.,Chebrolu hanumaiah institute of pharmaceutical science | Venkateswara Rao B.,Chebrolu hanumaiah institute of pharmaceutical science | Sasidhar R.L.C.,Chebrolu hanumaiah institute of pharmaceutical science
International Journal of Pharmacy and Technology | Year: 2012

The current study is to validate spectrophotometric method with UV detection for identification and determination of losartan potassium in respect of analytical parameters accuracy, precision, linearity. This simple, sensitive, accurate, economical and reproducible UV Spectrophotometric method can be applied for the determination of Losartan Potassium (LP) tablet dosage forms. The method is based on an observation that the aqueous solution of losartan potassium exhibits an absorbance maximum at 205nm and obeys Beer's law in the concentration range 1-5μg/ml. The statistical analysis data indicated a high level of precision for the proposed method. The coefficient of correlation was highly significant (0.995). The analytical results and recovery studies were validated statistically and found to be satisfactory. The proposed method has been applied successfully in the analysis of losartan potassium tablet formulation with good accuracy and precision. This method can be applied for the quality control of losartan potassium in tablets.


Vidyadhara S.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Sasidhar R.L.C.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Rao B.V.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Tejaswi K.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Reshma M.,Chebrolu Hanumaiah Institute of Pharmaceutical science
Oriental Journal of Chemistry | Year: 2014

A simple, fast, precise reverse phase isocratic high performance liquid chromatographic (HPLC) method has been developed for the simultaneous estimation of Olmesartan medoxomil (OM) and hydrochlorothiazide (HCTZ) in marketed formulations. Estimation of drugs in this combination was done with a C18 column [ODS UG 5 column, 250mm × 4.5mm] using mobile phase of composition acetonitrile and phosphate buffer (50:50 v/v, pH 6.8). The flow rate was 1.0 ml/min and the separation was monitored at 260nm. The retention time of HCTZ and OM were 3.6min and 4.3min respectively. The method was found to be linear over a range of 10-70ug/ml for OM and 6-42μg/ml for HCTZ. The method was validated according to the guidelines of International Conference on Harmonisation (ICH) and was successfully employed in the estimation of commercial formulations.


Nadella T.R.,Alkem Research center | Suryadevara V.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Lankapalli S.R.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Mandava V.B.R.,Krishna University | Bandarupalli D.,Vignan Pharmacy College
Journal of Pharmaceutical and Biomedical Analysis | Year: 2016

A simple, rapid, specific and precise liquid chromatography-tandem mass spectrophotometric (LC-MS/MS) method was developed and validated for quantification of busulfan, in human plasma. busulfan d8 was used as internal standard, added to plasma sample prior to extraction using acetonitrile as a precipitating agent. Chromatographic separation was achieved on phenomenex kinetex C18 column (50mm×2.1mm, 2.6μm) with acteonitrile: 10 mM ammonium formate buffer (80:20v/v) as an isocratic mobile phase with a flow rate of 0.5mLmin-1. Quantitation was performed by transition of 264.1→151.1 (m/z) for busulfan and 272.1→159.1 (m/z) for busulfan d8. The lower limit of quantitation was 0.2 ng mL-1 with a 100μL plasma sample. The concentrations of nine working standards showed linearity between 0.2 and 100 ng mL-1 (r2≥0.9986). Chromatographic separation was achieved within 2.0min. The average extraction recoveries of 3quality control concentrations were 92.52% for busulfan and 90.75% for busulfan d8. The coefficient of variation was ≤15% for intra- and inter-batch assays. The developed method was successfully applied for the determination of Busulfan pharmacokinetics after oral administration. © 2015 Elsevier B.V.


PubMed | Chebrolu Hanumaiah Institute of Pharmaceutical science
Type: Journal Article | Journal: Indian journal of pharmaceutical sciences | Year: 2013

In the present investigation an attempt has been made to increase therapeutic efficacy, reduced frequency of administration and improved patient compliance by developing controlled release matrix tablets of verapamil hydrochloride. Verapamil hydrochloride was formulated as oral controlled release matrix tablets by using the polyethylene oxides (Polyox WSR 303). The aim of this study was to investigate the influence of polymer level and type of fillers namely lactose (soluble filler), swellable filler (starch 1500), microcrystalline cellulose and dibasic calcium phosphate (insoluble fillers) on the release rate and mechanism of release for verapamil hydrochloride from matrix tablets prepared by direct compression process. Higher polymeric content in the matrix decreased the release rate of drug. On the other hand, replacement of lactose with anhydrous dibasic calcium phosphate and microcrystalline cellulose has significantly retarded the release rate of verapamil hydrochloride. Biopharmaceutical evaluation of satisfactory formulations were also carried out on New Zealand rabbits and parameters such as maximum plasma concentration, time to reach peak plasma concentration, area under the plasma concentration time curve(0-t) and area under first moment curve(0-t) were determined. In vivo pharmacokinetic study proves that the verapamil hydrochloride from matrix tablets showed prolonged release and were be able to sustain the therapeutic effect up to 24 h.


PubMed | Chebrolu Hanumaiah Institute of Pharmaceutical science
Type: Journal Article | Journal: International journal of pharmaceutical investigation | Year: 2015

The present study deals with the formulation of fast dissolving films of Rizatriptan benzoate that is used for the treatment of Migraine. The concept of fast-dissolving drug delivery emerged from the desire to provide patient with more conventional means of taking their medication.In the present research work, various trials were carried out using film forming agents such as maltodextrin, gum karaya and xanthan gum to prepare an ideal film. Emulsion evaporation method was used for the preparation of films. The prepared films were evaluated for weight uniformity, drug content, film thickness, folding endurance, dispersion test and curling. The in vitro dissolution studies were carried out using simulated salivary fluid (pH 6.8 phosphate buffer).About 97% of the drug was found to be released from the film within 10 min that is a desirable character for fast absorption. The drug excipient interaction studies carried out by differential scanning calorimetry analysis and Fourier transform infrared studies revealed that there were no major interactions between the drugs and excipients used for the preparation of films.Fast dissolving films of Rizatriptan benzoate prepared by emulsion evaporation technique were found to be suitable for eliciting better therapeutic effect in the treatment of migraine.

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