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Malhotra S.,Narsee Monjee Institute of Management and Higher Studies | Welling M.N.,Narsee Monjee Institute of Management and Higher Studies | Desai K.,Chauhan Institute of Science
Journal of Biomedical Materials Research - Part B Applied Biomaterials | Year: 2016

The toxicity of selenium (Se) as an antioxidant supplement in the treatment of arthritis is debatable. In this study, Dextrin stabilized Se nanoparticles (SeNP) of size 64 nm ± 0.158 were used to explore its effects as a potent antioxidant with reduced toxicity in both in vitro and in vivo. In vitro toxicity of SeNP was determined using cytotoxicity assay. In vitro interactions of SeNP with DNA and protein was established. Subacute toxicity of SeNP was studied. Wistar rats with complete freunds adjuvant induced arthritis were used. Various concentrations of SeNP per kg body weight were fed orally daily upto to 21 days. Arthritic profile based on paw swelling, histopathological changes in joints, blood indices, and antioxidant enzymes level in organs such as liver, kidney, and spleen were investigated. Dextrin-SeNP when interacted with NIH-3T3 cells showed 15% cytotoxicity at 100 µg/mL whereas, bulk Se showed 95% at the same concentration. SeNP at 250 µg/mL showed protective effect on DNA. Interaction of SeNP with BSA showed increase in quenching of BSA fluorescence. SeNP did not show any subacute toxicity at concentration as high as 5 mg/kg b.w. in Wistar rats. SeNP at a concentration of 250 µg/kg b.w. acted as potent anti-inflammatory agent and significantly reduced (p < 0.05) arthritis induced parameters. The enzymatic antioxidant levels in liver, kidney, and spleen were restored significantly (p < 0.05) at 500 µg/kg b.w. while CRP was regained to normal at concentration of 100 µg/kg b.w. concluding SeNP at 500 µg/kg b.w. can be a potential antiarthritic drug supplement. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 993–1003, 2016. © 2015 Wiley Periodicals, Inc. Source


Patil S.R.,Narsee Monjee Institute of Management and Higher Studies | Patil S.R.,Drug Monitoring Research Institute | Nerurkar K.K.,Drug Monitoring Research Institute | Kalamkar A.M.,Drug Monitoring Research Institute | And 4 more authors.
Journal of Mass Spectrometry | Year: 2012

An analytical method based on liquid-liquid extraction has been developed and validated for analysis of agomelatine in human plasma. Fluoxetine was used as an internal standard for agomelatine. A Betasil C18 (4.0 × 100 mm, 5 μm) column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatographic conditions and mass spectrometric detection in the positive ionization mode using an API-4000 system. The proposed method has been validated with linear range of 0.050-8.000 ng/ml for agomelatine. The intra-run and inter-run precision values are within 12.12% and 9.01%, respectively, for agomelatine at the lower limit of quantification level. The overall recovery for agomelatine and fluoxetine was 67.10% and 72.96%, respectively. This validated method was used successfully for analysis of plasma samples from a pharmacokinetic study. Copyright © 2012 John Wiley & Sons, Ltd. Source


Veer V.S.,Chauhan Institute of Science | Pingale S.G.,Chauhan Institute of Science | Mangaonkar K.V.,Chauhan Institute of Science
Journal of Liquid Chromatography and Related Technologies | Year: 2014

A rapid and sensitive ultra performance liquid chromatography method has been developed and validated for the simultaneous analysis of atovaquone and proguanil in rabbit plasma. The analytes were extracted from rabbit plasma using solid phase extraction. Pyrimethamine and chlorproguanil were used as internal standards for atovaquone and proguanil, respectively. A UPLC C18 column (100 × 2.1 mm, 1.7 m) provided chromatographic separation of analytes followed by detection with UPLC-UV detector. The proposed method has been validated with linear range of 600 to 70000 ng/mL for atovaquone and 30 to 3000 ng/mL for proguanil. The precision values are within 3.59% and 0.44% for atovaquone and proguanil, respectively, at LOQ level. The overall recovery for atovaquone and proguanil were 101.07% and 101.57%, respectively. Total elution time was as low as 2.5 min. This validated method has been used successfully for the analysis of plasma samples from a pharmacokinetic study. © 2014 Taylor and Francis Group, LLC. Source

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