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Wang K.-J.,Changzhou No. 1 Peoples Hospital | Shi D.-Q.,Nanjing University | Sun L.-S.,Nanjing University | Jiang X.,Nanjing University | And 5 more authors.
Chinese Medical Journal | Year: 2012

Background A number of studies have examined the association between estrogen receptor alpha (ESR-α) gene polymorphisms and bone mineral density (BMD), but previous studies of ESR-α gene XbaI (rs9340799) and PvuII (rs2234693) polymorphisms have been hampered by small sample size, regional restrictions and inconclusive results. Thus a meta-analysis is needed to assess their pooled effects. Methods This study reviewed all published articles indexed in Pubmed using the keywords in the title or abstract. All data were extracted independently by two reviewers using a standard form, the studies were meta-analyzed and minor discrepancies were resolved by authors' discussion. Results Twenty seven eligible studies involving 8467 women and 2032 men were identified. The XbaI and PvuII polymorphisms were significantly associated with BMD of the lumbar spine. XX and PP homozygotes had a protective effect in comparison with carriers of the x and p alleles, the effects were more significant in premenopausal women or Western women. At the femoral neck, the results were different. XX served as a protective factor in postmenopausal women, Western women, Western postmenopausal women, and men, while PP was likely to serve as a risk factor in Eastern women, Eastern postmenopausal women, and men. Conclusions The XbaI polymorphism is correlated to BMD at diverse skeletal sites. PP had a protective role for the lumbar spine but might be a risk factor for the femoral neck.

Ding W.-G.,Changzhou No. 1 Peoples Hospital | Liu J.-B.,Changzhou No. 1 Peoples Hospital | Wei Z.-X.,Changzhou No. 1 Peoples Hospital
Connective Tissue Research | Year: 2012

The purpose of this study was to compare the effects of spinal cord injury (SCI) and ovariectomy (OVX) on femoral fracture healing of later phase in young mice. Sixty young female C57 mice were randomized into three groups: SCI, OVX, and age-matched intact control. The femoral fracture was generated at 3 weeks after SCI or OVX. At 1 month after fracture, the femoral fracture area was evaluated through the healing status using radiograph; bone mineral density using dual X-ray absorptometry; callus formation and mineralization and neovascularization in callus using micro-computed tomography; biomechanical analysis using testing machine; and histology analysis by staining with hematoxylin-eosin stain. SCI mice showed lower bone mineral density in the femoral callus as compared with OVX mice. Callus geometric microstructural parameters of the femora in SCI mice were significantly lower than OVX mice. SCI induced significant changes of biomechanical parameters in the femoral fracture healing area. The callus formation and callus neovascularization in SCI mice were significantly lower than in OVX mice. SCI induces more deterioration of fracture healing in the femoral diaphysis than OVX. © 2012 Informa Healthcare USA, Inc.

Ding W.-G.,Changzhou No. 1 Peoples Hospital | Wei Z.-X.,Changzhou No. 1 Peoples Hospital | Liu J.-B.,Changzhou No. 1 Peoples Hospital
Connective Tissue Research | Year: 2011

Objective: To investigate angiogenesis of the tibial metaphysis in ovariectomized mice with microcomputed tomography, as well as to detect the expression of vascular endothelial growth factor (VEGF) in the metaphysis, and to explore the relationship between osteoporosis and local blood supply to bones. Methods: Sixty mice were randomly divided into an ovariectomy group (n 30) and a control group (n 30). Four weeks after ovariectomy, the mice were killed and the distribution of vessels in the tibial metaphysis was determined after silicone rubber perfusion. In addition, the expression of VEGF of the tibial metaphysis was immunohistochemically determined and bone mineral density, microarchitecture, and biomechanics were tested. Results: The bone mineral density, biomechanical parameters, number of microvessels, and expression of VEGF were significantly reduced in the tibial metaphysis of ovariectomized mice, whose bone microarchitecture was also disrupted. Conclusion: In this study, it was found that reduced local blood supply to the tibial metaphysis may be associated with ovariectomy-induced osteoporosis. © 2011 Informa Healthcare USA, Inc.

Zhang A.,Changzhou No. 1 Peoples Hospital | He S.,Changzhou No. 1 Peoples Hospital | Sun X.,Changzhou No. 1 Peoples Hospital | Ding L.,Changzhou No. 1 Peoples Hospital | And 2 more authors.
Cancer Cell International | Year: 2014

Wnt5a is classified as a non-transforming Wnt family member and plays complicated roles in oncogenesis and cancer metastasis. However, Wnt5a signaling in osteosarcoma progression remains poorly defined. In this study, we found that Wnt5a stimulated the migration of human osteosarcoma cells (MG-63), with the maximal effect at 100 ng/ml, via enhancing phosphorylation of phosphatidylinositol-3 kinase (PI3K)/Akt. PI3K and Akt showed visible signs of basal phosphorylation and elevated phosphorylation at 15 min after stimulation with Wnt5a. Pharmaceutical inhibition of PI3K with LY294002 significantly blocked the Wnt5a-induced activation of Akt (p-Ser473) and decreased Wnt5a-induced cell migration. Akt siRNA remarkably inhibited Wnt5a-induced cell migration. Additionally, Wnt5a does not alter the total expression and phosphorylation of β-catenin in MG-63 cells. Taken together, we demonstrated for the first time that Wnt5a promoted osteosarcoma cell migration via the PI3K/Akt signaling pathway. These findings could provide a rationale for designing new therapy targeting osteosarcoma metastasis. © 2014 Zhang et al.; licensee BioMed Central Ltd.

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