Sun C.-Y.,Changzhi Medical College |
Che Y.-J.,Changzhi Medical College |
Lu S.-J.,Central South University
Biotechnology Letters | Year: 2014
The healing of contaminated/infected bone defects is a significant clinical challenge. Here, a novel collagen scaffold composite encapsulating silver nanoparticles (AgNP) and bone morphogenetic protein 2 (BMP-2) was prepared to enhance the healing of infected bone defects. Collagen scaffolds conjugated with AgNP possessed strong antibacterial properties that were dependent on the release rate of Ag+. After introducing BMP-2, the BMP-2/AgNP/collagen scaffold composites did not adversely affect the adherence or proliferation of bone marrow-derived mesenchymal stromal cells (BMSCs). Differentiation of BMSCs toward osteoblasts was induced by the upregulation of RUNX2, osteopontin and osteonectin expression. BMP-2/AgNP/collagen scaffold composites, therefore, possess the antibacterial activity of AgNP and the osteoinductivity of BMP-2, making these composites an ideal pharmaceutical for the regeneration of bone in infected wounds. © 2014, Springer Science+Business Media Dordrecht.
Wang J.-F.,Changzhi Medical College
Cancer Research and Clinic | Year: 2013
Objective: To analyze the MRI data of misdiagnosed and missed diagnosed of breast lesions and their histopathological features. Methods: Data from 241 breast lesions within 121 patients were recruited in this study. The data included MRI images, ultrasounds and X-ray images were retrospectively interpreted by two radiologist and each lesion was assessed according to the BI-RADS classification. The pathologic features of miss or error diagnosed lesions on MRI were analyzed. Results: In 241 breast lesions (malignance 120, benign 121), 4 lesions were miss diagnosed on MRI. They were 2 intraductal papillomatosis and 2 fibroadenoma. All was benign. Twenty three lesions were misdiagnosed on MRI. Sixteen were overestimation, including 3 chronic inflammations, 3 sclerosing adenosis, 2 fibroadenoma, 4 fibrocystic changes with or without atypical ductal hyperplasia (ADH), 2 intraductal papilloma, 1 infiltration of pectoralis major muscle and 1 axillary lymphnode metastasis. Meanwhile, there were 7 lesions were underestimation. These lesions included 2 invasive ductal carcinomas, 1 mucinous adenocarcinoma, 2 DCIS and 1 blunt duct adenosis with ADH and focal cancerous, 1 inflammatory breast cancer underwent chemotherapy. The sensitivity and specificity and accuracy of breast MRI were 95.83% (115/120), 72.73% (88/121), 84.23% (203/241), respectively. MRI findings had no difference with respect to mammogram or ultrasound was 75.10% (181/241). Conclusion: MRI misdiagnosis and missed often occurs in smaller breast lesions, morphologic and hemodynamic malignant manifestation atypical, especially intraductal lesions. MRI diagnosis should be combined with physical examination, X-ray mammogram and ultrasound to improve diagnostic accuracy and reduce missed diagnosis.
Zhang X.Y.,Changzhi Medical College
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011
To explore the correlativity between HLA-DQ allele and primary Sjogren's syndrome(pSS) of the Han nationality in Shanxi province and to understand the pathogenesis of pSS at the gene level. Polymerase chain reaction sequence specific primers (PCR-SSP) technique was used to determine the alleles of HLA-DQA1 and HLA-DQB1 of pSS patients and healthy populations, and the difference in their HLA-DQA1 and HLA-DQB1 allelic frequencies were analyzed by using chi-square test and Fisher's exact test. (1) The gene frequency of HLA-DQA1*0501 in pSS patients was significantly higher than that in healthy controls(22.0% vs 12.0%, x(2);=7.087, P<0.05, RR=2.068). (2)The gene frequency of HLA-DQA1*0301/2 in pSS patients was significantly lower than that in controls(13.0% vs 24.5%, x(2);=8.681, P<0.05, RR=0.460). (3) The gene frequency of HLA-DQB1*0201 in pSS patients was significantly higher than that in controls(28.5% vs 18.5%, x(2);=5.563, P<0.05, RR=1.756). In Han nationality of Shanxi province, HLA-DQA1*0501 and HLA-DQB1*0201 alleles probably are susceptible genes of pSS, while HLA-DQA1*0301/2 allele probably is a protective gene of pSS.
Chang N.,Peking University |
Yi J.,Peking University |
Guo G.,Changzhi Medical College |
Liu X.,Peking University |
And 6 more authors.
Molecular and Cellular Biology | Year: 2010
In this study, we show that HuR destabilizes p16INK4 mRNA. Although the knockdown of HuR or AUF1 increased p16 expression, concomitant AUF1 and HuR knockdown had a much weaker effect. The knockdown of Ago2, a component of the RNA-induced silencing complex (RISC), stabilized p16 mRNA. The knockdown of HuR diminished the association of the p16 3′ untranslated region (3′UTR) with AUF1 and vice versa. While the knockdown of HuR or AUF1 reduced the association of Ago2 with the p16 3′UTR, Ago2 knockdown had no influence on HuR or AUF1 binding to the p16 3′UTR. The use of EGFP-p16 chimeric reporter transcripts revealed that p16 mRNA decay depended on a stem-loop structure present in the p16 3′UTR, as HuR and AUF1 destabilized EGFP-derived chimeric transcripts bearing wild-type sequences but not transcripts with mutations in the stem-loop structure. In senescent and HuR-silenced IDH4 human diploid fibroblasts, the EGFPp16 3′UTR transcript was more stable. Our results suggest that HuR destabilizes p16 mRNA by recruiting the RISC, an effect that depends on the secondary structure of the p16 3′UTR and requires AUF1 as a cofactor. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Xu L.,Central South University |
Jiang Y.,Central South University |
Zheng J.,Central South University |
Zheng J.,Changzhi Medical College |
And 4 more authors.
Human Pathology | Year: 2013
Nasopharyngeal carcinoma has a high incidence in southern China. The Wnt/β-catenin signaling pathway plays a major role in cancer development and progression. Our current study aims to determine the clinical significance of the Wnt/β-catenin pathway components such as β-catenin, cyclooxygenase 2, cyclin D1, c-Myc, and E-cadherin in 148 nasopharyngeal carcinomas by immunohistochemistry. We found that nasopharyngeal carcinoma stage T3+T4 had significantly higher expression of β-catenin, cyclooxygenase 2, cyclin D1, and c-Myc and lower expression of E-cadherin than nasopharyngeal carcinoma stage T1+T2 (P <.001, P <.05, respectively).There was significantly higher expression of β-catenin (P =.001) and cyclooxygenase 2 (P =.003) and lower expression of E-cadherin (P =.001) in nasopharyngeal carcinoma with lymph node metastasis than in nasopharyngeal carcinoma without lymph node metastasis. The expression of β-catenin in nasopharyngeal carcinoma was positively correlated with cyclooxygenase 2 (r = 0.458, P <.0001), cyclin D1 (r = 0.700, P <.0001), and c-Myc expression (r = 0.144, P =.006) but negatively correlated with E-cadherin expression (r = -0.601, P <.0001), respectively. The univariate analysis confirmed that overexpression of β-catenin and cyclooxygenase 2 and decreased expression of E-cadherin were significantly correlated with disease-free survival (P <.01, P <.05, respectively). Overexpression of β-catenin and cyclooxygenase 2 and reduced expression of E-cadherin significantly correlated with a poor prognosis (P =.005, P =.044, P =.019, respectively) by Kaplan-Meier survival curves and the log-rank test. Multivariate analysis indicated that high expression of β-catenin and decreased expression of E-cadherin were independent prognostic factors (P =.002, P =.011, respectively) regardless of TNM stage and lymph node status. In conclusion, the aberrant high expression of β-catenin and decreased expression of E-cadherin is associated with poor prognosis in nasopharyngeal carcinoma. © 2013 Elsevier Inc.