Sun A.,Jiangsu University |
Yu G.,Changzheng Hospital |
Dou X.,Jiangsu University |
Yan X.,Center for Health Research |
And 2 more authors.
Molecular Cancer | Year: 2015
Background: Gastric cardia adenocarcinoma (GCA) is the most aggressive subtype of gastric carcinoma. New molecular markers and therapeutic targets are needed for diagnosis, prognosis and treatment of GCA. This study is to establish the E3 ubiquitin ligase Nedd4-1 as a prognostic biomarker to predict the survival and guide the treatment of GCA patients. Methods: Expression of Nedd4-1 in 214 GCA tumor samples was detected by immunohistochemistry staining (IHC) using tissue microarray assay (TMA). Association of Nedd4-1 with cumulative survival of the TNM stages I-III patients and clinicopathological characteristics was statistically analyzed. The role of Nedd4-1 in gastric cancer cell migration and invasion were determined by transwell and wound healing assays. Results: Nedd4-1 is overexpressed in 83% of the GCA tumors. The 5-year survival rate in Nedd4-1 negative GCA patients is as high as 96%. Log-rank analysis indicated that overexpression of Nedd4-1 is inversely correlated with cumulative survival (χ 2 = 21.885, p <0.001). Multivariate logistic regression analysis showed that overexpression of Nedd4-1 is associated with an extremely low GCA survival rate with a hazard ratio (HR) = 0.068 (p = 0.008) in TNM stages I-III patients. Statistical analysis of association of Nedd4-1 overexpression with clinicopathological characteristics revealed that overexpression of Nedd4-1 is tightly associated with TNM stage (p < 0.001). Knockdown of Nedd4-1 in gastric cancer cell lines AGS and N87 dramatically inhibited the gastric cancer cell migration and invasion. Conclusions: Our results indicate that Nedd4-1 is an exceptional prognostic biomarker for GCA and suggest that Nedd4-1 may play an essential role in GCA metastasis. © 2014 Sun et al.; licensee BioMed Central Ltd.
Yu M.K.,Changzheng Hospital
Acta neurochirurgica. Supplement | Year: 2012
We prospectively studied the difference between head CT and MRI in the detection of midbrain injury at the acute stage, the characteristics of MRS in the midbrain, and its relationship to the prognosis. The aim of this study is to propose the imaging diagnosis and outcome assessment indicators for midbrain injury.According to the clinical diagnosis standard, 22 patients with midbrain injury were chosen as a midbrain injury group,and 20 cases with craniocerebral injury without brain stem injury as the control group,10 normal adult volunteers as the normal control group. CT was performed on days 1, 3, 5, and 7 respectively,and MRI and MRS within 7 days post-injury. All patients were followed up for 6 months post-injury.The positive diagnosis rate of 63.64% in MRI for midbrain injury was significantly higher than that of 13.63% found in CT. MRI showed that the location of the midbrain injury was closely associated with prognosis. The reduction of NAA/Cr or NAA/Cho ratio was more obvious and the prognosis of the patients poorer. Midbrain injury can be diagnosed more clearly and its severity or prognosis could also be evaluated by MRI and MRS.
Zhao Z.,Changzheng Hospital
Zhonghua nei ke za zhi | Year: 2014
RESULTS: (1)Compared with the normotensive group, the hypertensive group performed worse in the scores of MMSE, CDTs, the memory type cognitive function and the non-memory type cognitive function (MMSE:Z = -2.585, P = 0.010; CDTs:Z = -3.689, P < 0.001; memory type cognitive function:Z = -2.718, P = 0.007; non-memory type cognitive function: Z = -1.994, P = 0.046). (2) The incidences of cognitive impairment in the five groups were 3.6% (8/224), 6.6% (26/393), 16.6% (72/433), 19.7% (55/279) and 33.5% (64/191), respectively. (3) Compared with the group A, the cognitive function was significantly worse in the group E (MMSE:Z = 61.314, P < 0.001; CDTs: Z = 44.642, P < 0.001; memory type cognitive function:Z = 35.703, P < 0.001; non-memory type cognitive function:Z = 54.440, P < 0.001).CONCLUSIONS: Hypertension is a risk factor for the cognitive dysfunction. The incidence of cognitive dysfunction and the severity are positively associated with the duration of hypertension. In those with hypertension over 20 years, the cognitive dysfunction occurs much more obviously.OBJECTIVE: To investigate the relationship between duration of hypertension and cognitive function in adult hypertension patients.METHODS: A total of 224 subjects with normal blood pressure were enrolled in group A, and 1 296 patients with poorly controlled hypertension were further divided by the duration of hypertension into group B ( ≤ 5 years), group C (6-10 years), group D (11-20 years) and group E (> 20 years). Face-to-face surveys were conducted in all the subjects by trained physicians using Mini-Mental-State-Examination (MMSE) and Clock-Drawing-Test (CDT). The incidences of cognitive impairment were compared among the five groups and the relationship between duration of hypertension and cognitive function were analyzed by SPSS 18.0 software.
Liu Z.-T.,Shanghai JiaoTong University |
Fu P.-L.,Changzheng Hospital |
Wu H.-S.,Changzheng Hospital |
Zhu Y.,Changzheng Hospital
Knee | Year: 2012
Objective: To compare the results of primary total knee arthroplasty with patellar reshaping or resurfacing. Methods: One hundred thirty-three patients were randomized into patellar reshaping group and patellar resurfacing group. Patellar reshaping includes resecting the partial lateral facet of the patella and the osteophytes surrounding the patella, trimming the patella to match the trochlea of the femoral component. The minimum follow-up time was 7. years. The outcome was measured by anterior knee pain rate, Knee Society clinical score, and radiographs. Results: Eight patients in the reshaping group (12.5%) and 10 patients in the resurfacing group (14.7%) complained of anterior knee pain (P= 0.712). Meanwhile, there were no significant differences between the two groups in terms of total Knee Society score, Knee Society pain score, Knee Society function score, as well as anterior knee pain rate. Conclusions: With the numbers available, there was no significant difference between the groups treated with patellar reshaping or patellar resurfacing with regard to the KSS, anterior knee pain rate and radiographs. We prefer reshaping the patella to resurfacing the patella because the former preserves sufficient patellar bone stock and can easily be converted to patellar replacement if patients complain of recurrent anterior knee pain. © 2011 Elsevier B.V.
Fang W.,Changzheng Hospital |
Fa Z.,Shanghai Key Laboratory of Molecular Medical Mycology |
Liao W.,Changzheng Hospital |
Liao W.,Shanghai Key Laboratory of Molecular Medical Mycology
Fungal Genetics and Biology | Year: 2015
Cryptococcosis is a significant invasive fungal infection with noteworthy morbidity and mortality, primarily caused by Cryptococcus neoformans and Cryptococcus gattii. In China, C. neoformans var. grubii (especially molecular type VNI) is the most common variety in the environment and responsible for the majority of cryptococcal infections. C. gattii infections are quite rare in China and the primary molecular type is VGI, which is closely related to C. gattii isolates in Australia. Interestingly, the majority of cryptococcosis in China were reported in the HIV-uninfected patients (especially immunocompetent hosts). This unique phenomenon may be attributed to multiple polymorphisms in the genes encoding mannose-binding lectin (MBL) and Fc-gamma receptor 2B (. FCGR2B) in the Han population, the major ethnic group in China. Compared to immunocompromised patients, immunocompetent patients with cryptococcal meningitis often presented with more intense inflammatory responses and more severe neurological complications, but less fungal burdens and disseminated infection. The overall prognosis, which is independently associated with amphotericin B-based initial therapy, is similar between immunocompetent and immunocompromised patients. In addition, intrathecal administration of amphotericin B has been proved to be an effective adjunctive treatment for cryptococcosis in China. © 2014 The Authors.