Hengyang, China

Changsha Medical University

www.csmu.edu.cn
Hengyang, China

Changsha Medical UniversityCollege , China's governmental medical university for undergraduates,was founded by the famous educator Binsheng He in 1989. It is situated in Changsha, capital of Hunan Province and was upgraded to a medical university for undergraduates in 2005 by Ministry of Education. CSMU is under the jurisdiction of Hunan People's Government and the professional guidance of the Department of Public Health of the government. CSMU is listed in WHO's World Directory of Medical Schools and is welcoming students from all over the world. Wikipedia.


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Luo H.,Changsha Medical University | Han L.,Changsha Medical University | Tian S.,University of South China
Neuroscience Letters | Year: 2014

There is increasing evidence that nitric oxide may be involved in learning and memory. However, there remain comparatively few studies that have explored the relationship between nitric oxide signaling and fear extinction, an inhibitory learning model. In the present study, we tested the effects of nitric oxide synthase inhibitor l-NAME on three tone fear extinction tasks in rats. In task 1, rats received fear conditioning, extinction training and extinction test in the same context (AAA design). In task 2, rats received fear conditioning in context A, extinction training in context B and extinction test in context A (ABA design). In task 3, rats received fear conditioning in context A, extinction training and extinction test in context B (ABB design). l-NAME (10, 20 and 40. mg/kg) was injected intraperitoneally 30. min prior to extinction training in each task. Percent of time spent freezing was used to measure conditioned fear response. We found that l-NAME administrations had no effect on freezing in task 1 and 2 but produced a dose-dependent increase in task 3. Further results indicated that the increased freezing in task 3 was not attributed to state-dependency effects or nonspecific changes of locomotor activity that followed l-NAME injection. These results showed that l-NAME produced a task-dependent impairment of fear extinction, and implied that nitric oxide signaling was involved in memory process of certain extinction tasks. © 2014 Elsevier Ireland Ltd.


Peng L.,Changsha Medical University | Cao Z.,Hunan University | Liu L.,Changsha Medical University
Journal of Computational and Theoretical Nanoscience | Year: 2013

We presented a network comparison method for finding the conservative interaction regions in the across-species protein interaction networks (PINs). In the first part, firstly, we made use of the correlated matrix to represent the PINs. Then we standardized the matrix and changed it into a unique representation to facilitate to judge whether the subgraphs is isomorphic. Subsequently, we proposed a network comparison algorithm based on the correlated matrix, edge-betweenness and the maximal frequent subgraphs mining. We used the tag graphs library composed of the multiple across-species PINs as input data and mined the maximal frequent subgraphs. In the second part, we clustered and merged the similar but different and duplicate locally regions according to the similarity between them and the principle of single linkage clustering. In the end we analysed the resulting subgraphs and predicted the conservative interaction regions. The results showed the network comparison algorithm based on mining the frequent subgraphs can be successfully applied to discover the conservative interaction regions, that is to say, we can find the functional complexes. Furthermore, we can conclude the protein interactions existing in one species will be able to exist in the other species when the occurrences of the conservative regions meet or exceed the threshold of minimum support. Copyright © 2013 American Scientific Publishers All rights reserved.


Yang K.,Changsha Medical University | Yang K.,Hunan University | Zeng M.,Changsha Medical University
New Journal of Chemistry | Year: 2013

A novel multiresponsive hydrogel based on polyacrylamide functionalized with thymine derivatives has been demonstrated. In this study, thymines are grafted to the polyacrylamide chains by introducing thymines to the acrylamide precursors. Based on the photodimerization/cleavage of grafted thymines, the reversible sol-gel transition can be operated by alternate irradiation with 365 and 240 nm UV light. More importantly, thymine-functionalized polyacrylamide can combine with each other by the formation of a thymine(T)-Hg2+- thymine(T) complex to form a hydrogel in the presence of Hg2+. The formed hydrogel can gradually dissolve with the dissociation of the T-Hg 2+-T complex by a series of external stimuli such as pH, thymine and thiol compounds. Subsequently, Ru(bipy)3 2+ dye-doped silica nanoparticles (FSiNPs) are selected as model guests and loaded into hydrogel to demonstrate multiresponsive controlled release behavior. Moreover, the FSiNPs loaded with Hg2+-cross-linked hydrogel can be employed as a sensor for the detection of cysteine biomolecule. These results indicate that thymine-functionalized polyacrylamide can not only realize a multiresponsive gel-sol transition but also has a promising application in the field of controlled release and biochemical analysis. © 2013 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.


Tang L.,Changsha Medical University | Chen B.,Chinese University of Hong Kong | Ma B.,Walvax Biotechnology | Nie S.,Kunming Medical University
Genetics and Molecular Research | Year: 2014

In this study, we investigated the association between 5 interferon regulatory factor-5 (IRF5) single nucleotide polymorphisms (SNPs) and autoimmune diseases using the Medline citation index. Twenty-eight studies with 74 comparisons, including 16 rheumatoid arthritis (RA), 43 systemic lupus erythematous (SLE), 2 juvenile idiopathic arthritis (JIA), 6 multiple sclerosis (MS), and 5 systemic sclerosis (SSc) studies, were examined in the meta-analysis. The SNP rs2004640 was significantly associated with SLE, MS, and SSc, but not with JIA [odds ratio (OR) = 1.06, 95% confidence interval (CI) = 0.90-1.24, P = 0.48] or RA (OR = 1.03, 95%CI = 0.95-1.11, P = 0.44). A significant association was observed between rs2280714 and SLE, MS, and SSc, but not RA (OR = 1.01, 95%CI = 0.94-1.09, P = 0.80). Rs10954213 was associated with the pathogenesis of SLE, RA, MS, and SSc. rs2070197 and the exon 6 insertion were significantly associated with SLE. Haplotypes containing rs2004640T and rs2280714T were significantly associated with an increased risk of SLE, but not with RA. This meta-analysis certified that IRF5 polymorphisms confer susceptibility to SLE, MS, and SSc. To further confirm the correlations between polymorphisms of IRF5 and autoimmune disease susceptibility, studies involving a larger number of patients worldwide are necessary. © FUNPEC-RP.


Yang J.,Hunan City University | Li D.,Hainan Normal University | Peng L.,Changsha Medical University | Long K.,Hunan City University
Journal of Computational and Theoretical Nanoscience | Year: 2013

Based on the classification of the amino acid, the paper presents a mathematic representation of protein sequences, and then obtains a M matrix on the basis of mathematic expression. Then we computed the mathematic invariable according to the M matrix, namely five-dimensional feature vector. According to the angle between two vectors, we analyzed the similarity of 13 kinds of the original sequence of coronavirus N protein. With the software PHYLIP, we create a phylogenetic tree structure and compare the experimental results with the traditional one. The experimental results show that the mathematical model of this method is simple and have low computational complexity and better results. Such method of mathematic representation and similarity analysis of protein sequences is a new impetus for the comparison of protein sequences. Copyright © 2013 American Scientific Publishers.


Bao M.-H.,Changsha Medical University | Dai W.,Central South University | Li Y.-J.,Central South University | Hu C.-P.,Central South University
Canadian Journal of Physiology and Pharmacology | Year: 2011

It is proposed that myocardial cell apoptosis causes ventricular remodeling and heart failure. The aim of the present study was to determine the effects of rutaecarpine (Rut) on hypoxia-reoxygenation (H-R)-induced apoptosis in myocardial cell line H9c2, as well as the underlying mechanisms. Cultured H9c2 cells were exposed to hypoxia for 24 h, followed by 12 h reoxygenation. Rut (in concentrations of 0.1, 1, and 10 μmol/L) was added 1 h prior to H-R. Cell viability and lactate dehydrogenase were measured to evaluate the cell injuries. Apoptosis was evaluated by Hoechst 33258 staining and flow cytometry. NADPH oxidase activity was measured by assay kit; intracellular reactive oxygen species (ROS) generation was detected by 2′,7′-dichlorofluorescein diacetate; and Nox2, Nox4, and p47 phox mRNA and protein expression were analyzed by real-time PCR and Western blotting, respectively. The results showed that H-R significantly decreased cell viability and increased the lactate dehydrogenase release, as well as the apoptotic rate, concomitantly with enhanced NADPH oxidase activity. H-R also upregulated mRNA and protein expressions of Nox2, Nox4, and p47 phox and increased ROS production. Treatment with Rut markedly reversed these effects introduced by H-R. These results suggest that the protective effects of Rut against H-R-induced myocardial cell injury and apoptosis might, at least partly, be due to the inhibition of the NADPH oxidase - ROS pathway.


Xiao Z.,Changsha Medical University
Communications in Computer and Information Science | Year: 2011

Culture is an inseparable element of the sociolinguistic approach to language and language learning. A language cannot be taught and learned without coming to grips with its cultural content. It is well known that learning a foreign language is not only a process of understanding and using only the linguistic, but also the sociolinguistic level of cross-cultural communication as well. An inseparable element of the sociolinguistic approach to language and language learning is culture. A language cannot be taught without coming to grips with its cultural content. This eclectic paradigm entails designing learning resources that allow flexibility and variability while enabling students to learn through interaction with materials and other learners. © 2011 Springer-Verlag Berlin Heidelberg.


Qian M.,Changsha Medical University
Communications in Computer and Information Science | Year: 2011

Blended Learning modes create an ideal teaching environment and atmosphere by making good use of modern information and technology. As a result, the advantages of multimedia internet learning and regular classroom teaching are integrated well, bringing the teacher's guiding function (guidance, inspiring and monitoring) in teaching into full play and reflecting the student's subject position (initiative, enthusiasm and creativity) in learning. And the teaching concept "organic combination of the teacher's guiding function and the student's subject position" is actualized fully. © 2011 Springer-Verlag Berlin Heidelberg.


Luo H.-Q.,Changsha Medical University | Han L.,Changsha Medical University | Jiang Y.,Changsha Medical University
Asian Pacific Journal of Cancer Prevention | Year: 2014

Background: A meta-analysis was performed to examine the benefit/risk ratio for the addition of anti-HER MoAbs to chemotherapy in patients with advanced gastric and gastroesophageal cancer from six randomized phase II/III trials. Materials and Methods: We searched relative trials from Pubmed, EMBASE, Cochrane library databases, China National Knowledge Infrastructure databases, Google Scholar and the NIH ClinicalTrials. Primary outcomes were overall response rate (ORR), progression-free survival (PFS), overall survival (OS). Secondary outcomes were toxicities. All analyses were performed using STATA 12.0. Results: This meta-analysis included six randomized controlled trials (RCTs) with 2, 297 patients and we demonstrated that the anti-HER MoAbs arm did have a positive effect on ORR in the anti-HER MoAbs arm (OR 1.28, 95% CI 1.00-1.64, p=0.01). There was an increasing benefit regarding OS (HR 0.74, 95% CI 0.60-0.88, p<0.05) and PFS (HR 0.72, 95% CI 0.60-0.84, p<0.05) in the anti-HER2 subgroup, but a reduction of OS (HR 1.11, 95% CI 0.87-1.36, p<0.05) and PFS (HR 1.13, 95% CI 0.98 -1.28, P<0.05) in anti-EGFR subgroup. Some grade 3-4 toxicity had a significantly higher incidence in the anti-HER MoAbs arm. There was no significant publication bias for all endpoints. Conclusions: The addition of trstuzumab MoAb to chemotherapy for gastric and gastroesophageal cancer significantly improved outcome of OS and PFS endpoints, while other MoAbs led to no improvement in results. Some adverse events were increased in anti-HER MoAbs arm compared with the control.


Bao M.-H.,Changsha Medical University | Zhang Y.-W.,Central South University | Lou X.-Y.,Central South University | Cheng Y.,Central South University | Zhou H.-H.,Central South University
PLoS ONE | Year: 2014

Lectin-like low-density lipoprotein receptor 1 (LOX-1) is a receptor for oxidized low density lipoprotein (oxLDL) in endothelial cells. The activation of LOX-1 by oxLDL stimulates the apoptosis and dysfunction of endothelial cells, and contributes to atherogenesis. However, the regulatory factors for LOX-1 are still unclear. MicroRNAs are small, endogenous, non-coding RNAs that regulate gene expressions at a post-transcriptional level. The let-7 family is the second microRNA been discovered, which plays important roles in cardiovascular diseases. Let-7a and let-7b were predicted to target LOX-1 39-UTR and be highly expressed in endothelial cells. The present study demonstrated that LOX-1 was a target of let-7a and let-7b. They inhibited the expression of LOX-1 by targeting the positions of 310-316 in LOX-1 39-UTR. Over-expression of let- 7a and let-7b inhibited the oxLDL-induced endothelial cell apoptosis, NO deficiency, ROS over-production, LOX-1 upregulation and endothelial nitric oxide synthase (eNOS) downregulation. Moreover, we found that oxLDL treatment induced p38MAPK phosphorylation, NF-kB nuclear translocation, IkB degradation and PKB dephosphorylation. Let-7a or let- 7b over-expression attenuated these alterations significantly. The present study may provide a new insight into the protective properties of let-7a and let-7b in preventing the endothelial dysfunction associated with cardiovascular disease, such as atherosclerosis. © 2014 Bao et al.

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