Changsha, China
Changsha, China

Time filter

Source Type

Zhang H.-Y.,Hainan Medical College | Zheng S.-J.,Central South University | Zheng S.-J.,Dengfeng Peoples Hospital | Zhao J.-H.,Hainan Medical College | And 8 more authors.
Brain Research | Year: 2011

MicroRNAs (miRNAs) play an important role in the development, differentiation, proliferation, survival, and oncogenesis of cells and organisms including nervous system. However, the role of miRNAs in primary neurons of dorsal root ganglion (DRG) after injury was not clear. In this study, a miRNA microarray analysis was performed, and a total of 21 miRNAs were found to be down-regulated following unilateral sciatic nerve transection. The miR-144, miR-145, and miR-214 were further validated using quantitative reverse transcriptase PCR (qRT-PCR). Moreover, in situ hybridization (ISH) experiments using locked nucleic acid (LNA)-modified DNA oligonucleotide probes verified that miR-144, miR-145, and miR-214 were expressed in primary neurons of DRG and down-regulated following sciatic nerve transection. Predictions of potential miRNA targets involved were identified by performing a bioinformatics analysis. These predictions were tested using miRNA luciferase reporter vectors, with Robo2 and srGAP2 evaluated as the potential targets of miR-145 and miR-214, respectively. The role of miR-145 in cultured primary neurons was also investigated, and the result found that miR-145 miR-145 inhibited neurite growth and down-regulated Robo2 expression. Finding from this study suggested that miRNAs of DRG can mediated the course of regeneration including through Slit-Robo-srGAP signaling pathway after injury. © 2011 Elsevier B.V. All rights reserved.


Gao J.,Changsha Medical College | Deng S.,The No163 Hospital Of Pla | Wu J.,Wuhan No1 Hospital
Medical Journal of Wuhan University | Year: 2012

Objective: Technique to research optic radiation (OR) and the adjacent white matter structures using cerebral white matter fiber dissection technique and diffusion tensor imaging (DTI) for the diagnosis and treatment of ophthalmology diseases and neurosurgery diseases. Methods: Fifteen adult brain specimens were studied by cerebral white matter fiber dissection technique, special for optic radiation and adjacent to white matter structures; DTI technology was applied for imaging optic radiation fibers and adjacent to white matter structure in 10 healthy volunteers. Results: Meyer loop in the optic radiation and adjacent white matter fiber structures were clearly showed by cerebral white matter fiber dissection technique. The mean distances between the Meyer loop of OR and temporal thetemporal horn, temporal pole, ahead of the rear occipital pole were (4.44±0.55), (25.40±2.19), and (105.1±4.10) mm, respectively. The mean distances between the sagittal layer and the lateral wall of the lateral ventricle were (3.35±0.45) and (3.38±0.47) mm, respectively. No significant differences were found between brain white matter fiber dissection technique and DTI technique in these above measurements. Conclusion: There might be a consistence in the normal human optic radiation fibers detected by DTI and cerebral white matter fiber dissection technique, so this study provides a theoretic basis of DTI in the patients before performing operation in the temporal lobe.


Yang J.,China Agricultural University | Liu W.,China Agricultural University | Li B.,China Agricultural University | Yuan H.,China Agricultural University | And 2 more authors.
Journal of Environmental Sciences | Year: 2010

To improve the efficiency of backwashing in upflow biological aerated filter (BAF), a novel backwashing process named air-water siphon backwashing (AWSB) was proposed and applied on laboratory scale. The effects of backwashing on turbidity and suspended solid (SS) in backwashing effluent using this new backwashing process were compared with those of traditional backwashing process, namely air-water backwashing (AWB). In AWB, the turbidity of backwashing effluent maintained 100 NUT when consumed 60 min and 10.5 L water. However, in AWSB, it declined to 44 NUT, consumed 40 min, and7Lwater. The COD removal of BAF after backwashing was also analyzed. The average removal rate of COD in the reactor backwashed by AWB and AWSB was 89.34% and 90.91%, respectively. Compared with AWB, the backwashing interval had been prolonged 35%. The volume of backwashing water in that AWSB was only 66.7% of that in AWB. The results demonstrated that AWSB required less water and took shorter backwashing time relative to AWB to achieve the same turbidity of backwashing effluent, and AWSB was more effective in removing fouling than that of AWB. More significant advantage of AWSB was that the backwashing interval was effectively prolonged. © 2010 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.


Chen S.,Hunan University | Jian Z.,Hubei University of Medicine | Huang L.,Hubei University of Medicine | Xu W.,Shanghai University | And 9 more authors.
International Journal of Nanomedicine | Year: 2015

A mesoporous bioactive glass (MBG) surface modifed with poly(lactic-co-glycolic acid) (PLGA) electrospun fbrous scaffold for bone regeneration was prepared by dip-coating a PLGA electrospun fbrous scaffold into MBG precursor solution. Different surface structures and properties were acquired by different coating times. Surface morphology, chemical composition, microstructure, pore size distribution, and hydrophilicity of the PLGA-MBG scaffold were characterized. Results of scanning electron microscopy indicated that MBG surface coating made the scaffold rougher with the increase of MBG content. Scaffolds after MBG modifca-tion possessed mesoporous architecture on the surface. The measurements of the water contact angles suggested that the incorporation of MBG into the PLGA scaffold improved the surface hydrophilicity. An energy dispersive spectrometer evidenced that calcium-defcient carbonated hydroxyapatite formed on the PLGA-MBG scaffolds after a 7-day immersion in simulated body fuid. In vitro studies showed that the incorporation of MBG favored cell proliferation and osteogenic differentiation of human mesenchymal stem cells on the PLGA scaffolds. Moreover, the MBG surface-modifed PLGA (PLGA-MBG) scaffolds were shown to be capable of providing the improved adsorption/release behaviors of bone morphogenetic protein-2 (BMP-2). It is very signifcant that PLGA-MBG scaffolds could be effective for BMP-2 delivery and bone regeneration. © 2015, Chen et al.


PubMed | Hubei University of Medicine, Changsha Medical College, University of Texas at Austin, Central South University and Shanghai University
Type: | Journal: International journal of nanomedicine | Year: 2015

A mesoporous bioactive glass (MBG) surface modified with poly(lactic-co-glycolic acid) (PLGA) electrospun fibrous scaffold for bone regeneration was prepared by dip-coating a PLGA electrospun fibrous scaffold into MBG precursor solution. Different surface structures and properties were acquired by different coating times. Surface morphology, chemical composition, microstructure, pore size distribution, and hydrophilicity of the PLGA-MBG scaffold were characterized. Results of scanning electron microscopy indicated that MBG surface coating made the scaffold rougher with the increase of MBG content. Scaffolds after MBG modification possessed mesoporous architecture on the surface. The measurements of the water contact angles suggested that the incorporation of MBG into the PLGA scaffold improved the surface hydrophilicity. An energy dispersive spectrometer evidenced that calcium-deficient carbonated hydroxyapatite formed on the PLGA-MBG scaffolds after a 7-day immersion in simulated body fluid. In vitro studies showed that the incorporation of MBG favored cell proliferation and osteogenic differentiation of human mesenchymal stem cells on the PLGA scaffolds. Moreover, the MBG surface-modified PLGA (PLGA-MBG) scaffolds were shown to be capable of providing the improved adsorption/release behaviors of bone morphogenetic protein-2 (BMP-2). It is very significant that PLGA-MBG scaffolds could be effective for BMP-2 delivery and bone regeneration.


Wu Y.,Changsha Medical College | Li J.,Changsha Medical College | Kong Y.,Central South University | Chen D.,Central South University | And 2 more authors.
Journal of Central South University (Medical Sciences) | Year: 2013

Objective: To investigate the effect and mechanism of herpes simplex virus type 1 (HSV-1) based vector mediated interlukin-1 receptor antagonist (IL-1Rα) gene for knee osteoarthritis in rabbits. Methods: HSV-l vectors containing IL-1Rα genes were constructed and injected into the joint space of the osteoarthritis knee in rabbits for 4 weeks. The rabbits were sacrificed, and the knees were lavaged, dissected and the effect of transgene expression was analyzed. Levels of IL-1Rα and IL-1 expression in the recovered lavage fluids were measured with a cytokine ELISA kit. Cartilage from the lesion areas of medial femoral condyle and synovium were observed with hematoxylin and eosin (cartilage and synovium) and toluidine blue (cartilage). The blank control group was injected pHSV-LacZ vector into rabbit knees. Results: Intra-articular delivery of pHSV-IL-1Rα-LacZ resulted in a significant inhibition of IL-1 level and cartilage degradation compared with those in the blank control group (P<0.05). Conclusion: pHSV-LacZ is an ideal vector to mediate intra-articular gene delivery in the rabbit model of osteoarthritis. Continuous intra-articular expression of IL-1Rα can treat knee osteoarthritis by inhibiting IL-1.


Ma N.,Central South University | Ma N.,Changsha Medical College | Wang J.-F.,Changsha Medical College | Xu F.,Changsha Medical College | And 3 more authors.
Chinese Journal of New Drugs | Year: 2011

Resveratrol (3, 5, 4′-trihydroxystilbene) is a naturally occurring plant phytoalexin which is synthesized by several plants in response to adverse conditions, such as environmental stress or pathogenic attack. Growing evidence suggests that resveratrol exerts the preventing effect on human pathological processes, such as inflammation, atherosclerosis, and carcinogenesis, so it is of catholic concern. Therefore, resveratrol derivatives and analogues have caused great attention both in home and abroad. Relative literature in the last few years about its pharmacological activities and analysis methods were summarized and analyzed in this paper. These compounds have multiple biological and pharmacological activities like resveratrol; but some are superior over resveratrol in activity, selectivity and stability, and have become a research focus in recent years.


Mou J.,Changsha Medical College | Zhao S.,Changsha Medical College | Wu D.,Tianxin Center For Disease Control And Prevention In Changsha Email | Luo J.,Central South University | And 2 more authors.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2014

OBJECTIVE: To study the influence of mediating/moderating effects of health skills on the relations between health knowledge and health behaviors in college students.METHODS: Stratified cluster random sampling was used among 2 181 students, selected in several colleges in Changsha, Hunan province. EpiData 3.0 was adopted to establish the database. Correlation and regression analyses were performed by SPSS 17.0.RESULTS: Positive correlations were seen on: 1) Knowledge and skills on health (r = 0.592, P < 0.01), 2) Knowledge and behaviors on health (r = 0.647, P < 0.01), 3) Health skills and health behaviors(r = 0.629, P < 0.01). The mediating effect of health skills on the relations between health knowledge and health behaviors was significant (34.55%). The interaction effect of "health skill × (times) related knowledge" was significant (β = -0.093, t = - 5.212, P = 0.000). New variables that were produced by the interaction also reached significant level (Δ R(2) = 0.006, P = 0.000), resulted in increasing the explanation function to health behaviors by 0.6%.CONCLUSION: Health skills could partially mediate the effects and moderate the relationship between health knowledge and health behaviors among college students.


Liu R.,Changsha Medical College | Li H.-Y.,Changsha Medical College | Yang C.-X.,Central South University
Zhongnan Daxue Xuebao (Ziran Kexue Ban)/Journal of Central South University (Science and Technology) | Year: 2012

A customer selection strategy with comprehensive long-term gains was put forward. The procedures are as follows. The selection criteria is based on the interaction and potential long-term value of the customer. The potential value of the customer is calculated according to his interaction history, credibility and active level. These parameters are selected as evaluation parameters for customer's long-term value calculation, and then the profit calculation method accepting the customer service requests as well as the loss calculation method of rejecting service requests are given. The results show that, compared with traditional methods which only focus on current profit maximization, this strategy is better in the long-term profit by more than 30%, and the longer inspection, the more obvious effects can be obtained, which indicates the superiority of the strategy.


PubMed | Changsha Medical College
Type: Journal Article | Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences | Year: 2013

To investigate the effect and mechanism of herpes simplex virus type 1 (HSV-1) based vector mediated interlukin-1 receptor antagonist (IL-1R) gene for knee osteoarthritis in rabbits.HSV-1 vectors containing IL-1R genes were constructed and injected into the joint space of the osteoarthritis knee in rabbits for 4 weeks. The rabbits were sacrificed, and the knees were lavaged, dissected and the effect of transgene expression was analyzed. Levels of IL-1R and IL-1 expression in the recovered lavage fluids were measured with a cytokine ELISA kit. Cartilage from the lesion areas of medial femoral condyle and synovium were observed with hematoxylin and eosin (cartilage and synovium) and toluidine blue (cartilage). The blank control group was injected pHSV-LacZ vector into rabbit knees.Intra-articular delivery of pHSV-IL-1R-LacZ resulted in a significant inhibition of IL-1 level and cartilage degradation compared with those in the blank control group (P<0.05).pHSV-LacZ is an ideal vector to mediate intra-articular gene delivery in the rabbit model of osteoarthritis. Continuous intra-articular expression of IL-1R can treat knee osteoarthritis by inhibiting IL-1.

Loading Changsha Medical College collaborators
Loading Changsha Medical College collaborators