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Lin X.L.,Changning District Central Hospital
Zhonghua fu chan ke za zhi | Year: 2011

To investigate the relationship between vaginal and intestinal candida in patients with vulvovaginal candidiasis by using microbiological and molecular methods. The samples of vaginal discharge and anal swabs were collected from 148 cases with vulvovaginal candidiasis, followed by fungal culture, identification, purification and genome DNA extraction. The genome sequences from respective locations were aligned and typed according to their homology analyzed by internal transcribed spacer (ITS) PCR and random amplified polymorphic DNA (RAPD) PCR. Patients with vulvovaginal infection or those with infections in intestine and vulvovagina were pooled respectively, while the recurrent incidences after local anti-fungal treatments were analyzed. Candida albicans is the dominant pathogen in 148 cases with vulvovaginal candidiasis (91.9%, 136/148); 33.1% (49/148) of patients with vulvovaginal candidiasis were infected in both intestine and vulvovagina. While 92% (22/24) of patients with intestinal and vaginal candida infection showed high homology. The recurrent rate of patients with vulvovaginal candidiasis complicated with concurrent intestinal candida infection (7/14) was significantly higher than that of solo vaginal infected patients [21% (6/29)] after vaginal treatment (P<0.05). The infection of vulvovaginal candidiasis is highly associated with the concurrent infection of intestinal candida. The recurrent rate is high in patients with vulvovaginal candidiasis with concurrent infection of intestinal candida after vaginal treatment. The general management to those patients infected by both vulvovaginal and intestinal candida is necessary in reducing the recurrence of the disease.

Zuo X.,Fudan University | Zuo X.,Tongji University | Shen A.,Tongji University | Chen M.,Changning District Central Hospital
Australian and New Zealand Journal of Obstetrics and Gynaecology | Year: 2012

Aims To analyse the optimal laparoscopic surgical techniques for the treatment of interstitial pregnancy to minimise bleeding during the operative procedure and the safety of the subsequent pregnancy. Methods Advanced bipolar coagulator was used to achieve haemostasis. Results The mean gestational age was 55 ± 5.1 days. All 17 women with an interstitial pregnancy were successfully treated by laparoscopic surgery without any complication. No surgery was converted to laparotomy. The mean pre-operative beta-human chorionic gonadotropin (β-hCG) serum concentration was 14 696 ± 11 705 mIU/mL. This value decreased to 1911 ± 1769 mIU/mL at 3-day post-operation. Among women who underwent laparoscopic surgery, a cornual resection was performed in 16 (94.1%) cases. One (5.8%) woman underwent a laparoscopic evacuation of the conceptus and received a local injection of 10 mg methotrexate. The volume of blood loss was <25 mL in 16 cases. However, one woman experienced a rupture at the beginning of the operation and lost 250 mL of blood. The mean hospital stay was 4.5 days. Four of the nine women who chose to retain their reproductive function had subsequent normal pregnancies, but all received an elective caesarean delivery prior to labour. Conclusions The laparoscopic management of women with unruptured interstitial pregnancy can frequently be performed without haemorrhage or complication using advanced bipolar coagulation. The small sample of successful subsequent pregnancies demonstrates the safety and effectiveness of this technique, but this finding should be confirmed by further investigations. © 2012 The Royal Australian and New Zealand College of Obstetricians and Gynaecologists.

Bian L.,Shanghai University | Lu X.,Changning District Central Hospital | Xu J.,Shanghai University | Chen J.,Shanghai University | And 6 more authors.
Journal of Fluorine Chemistry | Year: 2013

The synthesis of 2-perfluoroalkylated benzoxazolines and benzothiazolines was easily achieved under mild conditions in high yields and purity. The protocol uses readily available methyl 2-perfluoroalkynoates as fluorine-containing building block, and furnished the expected product in good to excellent yields in MeOH under reflux for 10 h. This novel synthetic protocol is efficient and general. A plausible mechanism for this process is proposed. © 2013 Elsevier B.V. All rights reserved.

Zhu T.-q.,Shanghai JiaoTong University | Zhang Q.,Shanghai JiaoTong University | Ding F.-h.,Shanghai JiaoTong University | Qiu J.-p.,Gong li Hospital | And 8 more authors.
Chinese Medical Journal | Year: 2013

Background No randomized trial has been performed to compare the efficacy of an intracoronary bolus of tirofiban versus urokinase during primary percutaneous coronary intervention (PCI). We investigated whether the effects of adjunctive therapy with an intracoronary bolus of urokinase was noninferior to the effects of an intracoronary bolus of tirofiban in patients with ST-elevation myocardial infarction (STEMI) undergoing PCI. Methods A total of 490 patients with acute STEMI undergoing primary PCI were randomized to an intracoronary bolus of tirofiban (10 μg/kg; n=247) or urokinase (250 kU/20 ml; n=243). Serum levels of P-selectin, von Willebrand factor (vWF), CD40 ligand (CD40L), and serum amyloid A (SAA) in the coronary sinus were measured before and after intracoronary drug administration. The primary endpoint was the rate of complete (≥70%) ST-segment resolution (STR) at 90 minutes after intervention, and the noninferiority margin was set to 15%. Results In the intention-to-treat analysis, complete STR was achieved in 54.4% of patients treated with an intracoronary bolus of urokinase and in 60.6% of those treated with an intracoronary bolus of tirofiban (adjusted difference: -7.0%; 95% confidence interval: -15.7% to 1.8%). The corrected TIMI frame count of the infarct-related artery was lower, left ventricular ejection fraction was higher, and the 6-month major adverse cardiac event-free survival tended to be better in the intracoronary tirofiban group. An intracoronary bolus of tirofiban resulted in lower levels of P-selectin, vWF, CD40L, and SAA in the coronary sinus compared with an intracoronary bolus of urokinase after primary PCI (P<0.05). Conclusions An intracoronary bolus of urokinase as an adjunct to primary PCI for acute STEMI is not equally effective to an intracoronary bolus of tirofiban with respect to improvement in myocardial reperfusion assessed by STR. This may be caused by less reduction in coronary circulatory platelet activation and inflammation.

Han J.,Shanghai University | Li L.,Changning District Central Hospital | Shen Y.,Shanghai University | Chen J.,Shanghai University | And 6 more authors.
European Journal of Organic Chemistry | Year: 2013

One-pot, two-step cyclization of o-aminophenyl ketones 1 and methyl perfluoroalk-2-ynoates 2 takes place in the presence of copper(I) as catalyst and diisopropylethylamine as base, leading to 2-perfluoroalkylated quinolines. Compared with previous methods that have been used to generate quinoline skeletons, this technique uses inexpensive copper salt as catalyst, methyl perfluoroalk-2-ynoate as fluorinated building block and proceeds smoothly under mild conditions, which makes the transformation both sustainable and practical. Copper(I)-catalyzed, one-pot, two-step cyclization of o-aminophenyl ketones 1 and methyl perfluoroalk-2-ynoates 2 in the presence of diisopropylethylamine leading to 2-perfluoroalkylated quinolines 3 proceeds smoothly under mild conditions. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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