Changhae Ethanol Co.

Jeonju, South Korea

Changhae Ethanol Co.

Jeonju, South Korea
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Patent
Gs Caltex Corporation and Changhae Ethanol Co. | Date: 2013-05-14

Provided is a method for producing bioproducts, including the steps of: culturing a first microorganism to produce bioalcohol; hydrolyzing the first microorganism; separating the bioalcohol; obtaining wastes from the hydrolyzed bioalcohol fermentation; and inoculating a second microorganism into the wastes from the hydrolyzed bioalcohol fermentation.


Patent
Changhae Ethanol Co. and Gs Caltex Corporation | Date: 2010-09-13

Disclosed is a preparation method for bio-fuel materials and bio-chemicals comprising the following steps: preparing a medium comprising fermentation waste generated in an alcohol production process; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated. More specifically, the preparation method for bio-fuel materials and bio-chemicals comprises the following steps: fermenting hexoses from a mixture of pentoses and hexoses to produce an ethanol fermentation broth; separating and purifying the ethanol fermentation broth; preparing a medium comprising the fermentation waste produced in the separation and purification step; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated.


Patent
GS Caltex Corporation and Changhae Ethanol Co. | Date: 2015-03-25

Provided is a method for producing bioproducts, including the steps of: culturing a first microorganism to produce bioalcohol; hydrolyzing the first microorganism; separating the bioalcohol; obtaining wastes from the hydrolyzed bioalcohol fermentation; and inoculating a second microorganism into the wastes from the hydrolyzed bioalcohol fermentation.


Han M.,Changhae Ethanol Co. | Kim Y.,Changhae Ethanol Co. | Kim S.W.,Korea University | Choi G.-W.,Changhae Ethanol Co.
Journal of Chemical Technology and Biotechnology | Year: 2011

Background: Current ethanol production processes using crops such as corn and sugar cane are well established. However, the utilization of cheaper biomasses such as lignocellulose could make bioethanol more competitive with fossil fuels while avoiding the ethical concerns associated with using potential food resources. Results: Oil palm empty fruit bunches (OPEFB), a lignocellulosic biomass, was pretreated using NaOH to produce bioethanol. The pretreatment and enzymatic hydrolysis conditions were evaluated by response surface methodology (RSM). The optimal conditions were found to be 127.64 °C, 22.08 min, and 2.89 mol L-1 for temperature, reaction time, and NaOH concentration, respectively. Regarding enzymatic digestibility, 50 FPU g-1 cellulose of cellulase was selected as the test concentration, resulting in a total glucose conversion rate (TGCR) of 86.37% using the Changhae Ethanol Multi Explosion (CHEMEX) facility. Fermentation of pretreated OPEFB using Saccharomyces cerevisiae resulted in an ethanol concentration of 48.54 g L-1 at 20% (w/v) pretreated biomass loading, along with simultaneous saccharification and fermentation (SSF) processes. Overall, 410.48 g of ethanol were produced from 3 kg of raw OPEFB in a single run, using the CHEMEX-50 L reactor. Conclusion: The results presented here constitute a significant contribution to the production of bioethanol from OPEFB. © 2011 Society of Chemical Industry.


Kang H.-W.,Changhae Ethanol Co. | Kim Y.,Changhae Ethanol Co. | Kim S.-W.,Korea University | Choi G.-W.,Changhae Ethanol Co.
Bioprocess and Biosystems Engineering | Year: 2012

In cellulosic ethanol production, use of simultaneous saccharification and fermentation (SSF) has been suggested as the favorable strategy to reduce process costs. Although SSF has many advantages, a significant discrepancy still exists between the appropriate temperature for saccharification (45-50 °C) and fermentation (30-35 °C). In the present study, the potential of temperature-shift as a tool for SSF optimization for bioethanol production from cellulosic biomass was examined. Cellulosic ethanol production of the temperature-shift SSF (TS-SSF) from 16 w/v% biomass increased from 22.2 g/L to 34.3 g/L following a temperature shift from 45 to 35 °C compared with the constant temperature of 45 °C. The glucose conversion yield and ethanol production yield in the TS-SSF were 89.3% and 90.6%, respectively. At higher biomass loading (18 w/v%), ethanol production increased to 40.2 g/L with temperatureshift time within 24 h. These results demonstrated that the temperature-shift process enhances the saccharification ratio and the ethanol production yield in SSF, and the temperatureshift time for TS-SSF process can be changed according to the fermentation condition within 24 h. © Springer-Verlag 2011.


Moon S.-K.,Changhae Ethanol Co. | Kim S.W.,Korea University | Choi G.-W.,Changhae Ethanol Co.
Journal of Biotechnology | Year: 2012

A continuous process was employed to improve the volumetric productivity of bioethanol production from cassava mash containing sludge and to simplify the process of ethanol production from cassava. After raw cassava powder was liquefied, it was used directly in a continuous process without sludge filtration or saccharification. A fermentor consisting of four linked stirrer tanks was used for simultaneous saccharification and continuous fermentation (SSCF). Although the mash contained sludge, continuous fermentation was successfully achieved. We chose the dilution rate on the basis of the maximum saccharification time; the highest volumetric productivity and ethanol yield were observed at a dilution rate of 0.028h -1. The volumetric productivity, final ethanol concentration, and % of theoretical ethanol yield were 2.41g/Lh, 86.1g/L, and 91%, respectively. This SSCF process using the self-flocculating yeast Saccharomyces cerevisiae CHFY0321 illustrates the possibility of realizing cost-effective bioethanol production by eliminating additional saccharification and filtration processes. In addition, flocculent CHFY0321, which our group developed, showed excellent fermentation results under continuous ethanol production. © 2011 Elsevier B.V.


Moon S.-K.,Changhae Ethanol Co. | Wee Y.-J.,Yeungnam University | Choi G.-W.,Changhae Ethanol Co.
Journal of Bioscience and Bioengineering | Year: 2012

Fermentation-derived lactic acid has several potential industrial uses as an intermediate carbon chemical and a raw material for biodegradable polymer. We therefore undertook the identification of a novel bacterial strain that is capable of producing high concentrations of lactic acid and has potential commercial applications. A novel l(+)-lactic acid producing bacterium, Lactobacillus paracasei subsp. paracasei CHB2121 was isolated from soil obtained near an ethanol production factory and identified by 16S rRNA gene sequence analysis and characterization using an API 50 CHL kit. L. paracasei subsp. paracasei CHB2121 efficiently produced 192. g/L lactic acid from medium containing 200. g/L of glucose, with 3.99. g/(L·h) productivity, and 0.96. g/g yield. In addition, the optical purity of the produced lactic acid was estimated to be 96.6% l(+)-lactic acid. The newly identified L. paracasei subsp. paracasei CHB2121 efficiently produces high concentrations of lactic acid, and may be suitable for use in the industrial production of lactic acid. © 2012 The Society for Biotechnology, Japan.


Moon S.-K.,Changhae Ethanol Co. | Wee Y.-J.,Yeungnam University | Choi G.-W.,Changhae Ethanol Co.
Journal of Industrial Microbiology and Biotechnology | Year: 2014

The by-products of bioethanol production such as thin stillage (TS) and condensed distillers solubles (CDS) were used as a potential nitrogen source for economical production of lactic acid. The effect of those by-products and their concentrations on lactic acid fermentation were investigated using Lactobacillus paracasei CHB2121. Approximately, 6.7 g/L of yeast extract at a carbon source to nitrogen source ratio of 15 was required to produce 90 g/L of lactic acid in the medium containing 100 g/L of glucose. Batch fermentation of TS medium resulted in 90 g/L of lactic acid after 48 h, and the medium containing 10 % CDS resulted in 95 g/L of lactic acid after 44 h. Therefore, TS and CDS could be considered as potential alternative fermentation medium for the economical production of lactic acid. Furthermore, lactic acid fermentation was performed using only cassava and CDS for commercial production of lactic acid. The volumetric productivity of lactic acid [2.94 g/(L·h)] was 37 % higher than the productivity obtained from the medium with glucose and CDS. © 2014, Society for Industrial Microbiology and Biotechnology.


Jeong J.-S.,Changhae Ethanol Co. | Jeon H.,Changhae Ethanol Co. | Ko K.-M.,Changhae Ethanol Co. | Chung B.,Chonbuk National University | Choi G.-W.,Changhae Ethanol Co.
Renewable Energy | Year: 2012

Renewable energy is now increasingly becoming the center of interest as a solution to problems of fossil fuel. Bioethanol, especially, is able to substitute petroleum as fuel; making it a viable and promising renewable energy. Dehydration process is crucial for production of fuel ethanol. PSA (Pressure Swing Adsorption) process is most widely used due to its energy and cost efficiency. In this research, anhydrous ethanol was produced through various processes such as; two-bed, multi-tube bed, two-step process, and three-bed for analysis and comparison of each process. Through this study, two-bed process and multi-tube bed process were both shown to produce 99.5wt% anhydrous ethanol from 87.0wt% ethanol. However, multi-tube bed process has lower energy consumption. Two-step bed process has advantage of being able to produce anhydrous ethanol from input ethanol concentration as low as 83.1wt%. Lastly, three-bed process allowed for longer regeneration time, making the process very stable and with higher yield due to less lost time in cycle switching. © 2011 .


Han M.,Changhae Ethanol Co. | Kang K.E.,Changhae Ethanol Co. | Kim Y.,Changhae Ethanol Co. | Choi G.-W.,Changhae Ethanol Co.
Process Biochemistry | Year: 2013

We developed a new pretreatment process for producing high-efficiency bioethanol from a lignocellulosic biomass. Barley straw was pretreated with sodium hydroxide in a twin-screw extruder for continuous pretreatment. The biomass to ethanol ratio (BTER) for optimal pretreatment conditions was evaluated by response surface methodology. Simultaneous saccharification and fermentation (SSF) was conducted to investigate the BTER with 30 FPU/g cellulose of enzyme and 7% (v/v) yeast (Saccharomyces cerevisiae CHY 1011) using 10% (w/v) pretreated biomass under various pretreatment conditions. The maximum BTER was 73.00% under optimal pretreatment conditions (86.61 °C, 0.58 M, and 84.79 mL/min for temperature, sodium hydroxide concentration, and solution flow rate, respectively) and the experimental BTER was 70.01 ± 0.59%. SSF was performed to investigate the optimal enzyme and biomass dosage. As a result, maximum ethanol concentration and ethanol yield were 46.00 g/L and 77.36% at a loading pretreated biomass of 20% with 30 FPU/g cellulose of the enzyme dosage for barley straw to bioethanol. These results are a significant contribution to the production of bioethanol from barley straw. © 2013 Elsevier Ltd. All rights reserved.

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