Zhang S.-F.,Changchun Medicial College
Chinese Journal of Biologicals | Year: 2012
Objective: To investigate the inhibition of proliferation and induction of programmed death of Hep-2 cells cultured in vitro by FTY720. Methods: Hep-2 cells were treated with FTY720 at various concentrations (800, 1 600 and 3 200 ng/ml) for 48 h, then observed for proliferation activity by MTT assay, for morphology by Switzerland-Giemsa staining, and for cell cycle and apoptosis by flow cytometry. Results: FTY720 showed dose-dependent inhibitory effect on proliferation of Hep-2 cells. The inhibiting rate of cell proliferation by FTY720 at a concentration of 3 200 ng/ml was (60.900 ± 0.071)% (P < 0.05). Programmed death was observed in the cells treated with FTY720 which arrested the cells at G 2 phase. The apoptosis rate of cells treated with 1 600 ng/ml FTY720 increased significantly (P < 0.05). Conclusion: The FTY720 at a certain concentration inhibited the proliferation, regulated the cell cycle and induced the programmed death of Hep-2 cells cultured in vitro.
Zhang S.-F.,Changchun Medicial College |
Sun J.-F.,Changchun Medicial College |
Zhang L.,Changchun Medicial College |
Song Y.-M.,Changchun Medicial College
Chinese Journal of Biologicals | Year: 2010
Objective: To investigate the inhibition of growth and induction of apoptosis of human laryngeal cancer Hep-2 cells by curcumin. Methods: Hep-2 cells were cultured in media containing curcumin at various concentrations (3, 6, 12.5 and 25 μmol/L) for 24 and 48 h separately. The effects on cell proliferation was determined by MTT method, on cell viability by trypan blue staining, on distribution of cell cycle and cell apoptosis by flow cytometry, and on cell DNA by DNA agarose gel electrophoresis. Results: Curcumin inhibited the proliferation of Hep-2 cells significantly in a time- and dose-dependent manner, decreased the cell viability in a dose-dependent manner, arrested the cells at G2/M phase and induced obvious apoptosis peaks as well as apoptosis rate in a dose- and time-dependent manner. Typical DNA-Ladder was formed in the cell DNA treated with curcumin. Conclusion: Curcumin significantly inhibited the proliferation and viability, and induced the apoptosis of Hep-2 cells.