Xue H.,Jilin University |
Zhang X.-Y.,Jilin University |
Liu J.-M.,Jilin University |
Song Y.,Jilin University |
And 3 more authors.
Brain Research | Year: 2013
After spinal cord injury (SCI), a series of complex pathophysiological processes follows the initial injury. Because inflammation plays a key role in this secondary pathology damage, anti-inflammatory drug treatment may reduce secondary damage and protect neurons after SCI. Though nordihydroguaiaretic acid (NDGA) can inhibit inflammatory responses, its potential roles in neuroprotection and anti- inflammation in an SCI model have not been studied. In this study, we investigated the anti-inflammatory effects of NDGA in SCI. First, histopathological alterations were evaluated with hematoxylin/eosin (HE) and Nissl staining, showing an increased number of neurons after NDGA administration. Additionally, the extent of secondary damage was assessed by TUNEL assay and measurement of astrocyte proliferation. The data showed that the numbers of apoptotic cells and the proliferative extent of astrocytes were significantly decreased by the use of NDGA. The anti-inflammatory effect of NDGA was evaluated by measuring myeloperoxidase (MPO) levels as an indicator of neutrophil activity, macrophage/microglia numbers, and expression of inflammatory cytokines including IL-1β and TNF-α. NDGA treatment significantly decreased the MPO level and the number of macrophages/microglia. In addition, NDGA also suppressed the expression of IL-1β and TNF-α after SCI. These data suggest that anti-inflammatory action by NDGA can reduce secondary damage after SCI © 2013 Elsevier B.V. All rights reserved.
Xue H.,Jilin University |
Zhang X.-Y.,Jilin University |
Liu J.-M.,Jilin University |
Song Y.,Jilin University |
And 3 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2013
Bridging strategies are essential for spinal cord repair in order to provide a physical substrate allowing axons to grow across the site of spinal cord lesions. In this study, we have evaluated the therapeutic effects of adding amniotic epithelial cells to a unidirectionally oriented acellular muscle scaffold and have compared this with the effect of a scaffold alone. Chemically extracted acellular muscles, with or without amniotic epithelial cells, were implanted into the lateral hemisected adult rat thoracic spinal cord. Control rats were similarly injured. After 4 weeks, the acellular muscle scaffolds were found to be well integrated with the host tissue. The chemically extracted acellular muscle scaffold seeded with amniotic epithelial cells promoted axonal growth in a distinctly organized and linear fashion, induced sprouting of calcitonin gene-related peptide positive axons, and was not associated with an astrocyte response. Compared with acellular muscle scaffolds alone, the addition of amniotic epithelial cells further promoted the remyelination of nerve fibers, sprouting of 5-hydroxytryptamine nerve fibers, relays of cortical motor-evoked potential and cortical somatosensory-evoked potential, and functional recovery. All these data together suggest that co-implantation of chemically extracted acellular muscle with amniotic epithelial cells may constitute a valuable approach to study and/or develop therapies for spinal cord injury. © 2012 Wiley Periodicals, Inc.
Zhang Y.-Y.,Nanjing Normal University |
Zhang Y.-Y.,Changchun Medical College |
Meng C.,Nanjing Normal University |
Zhang X.-M.,Jilin University |
And 7 more authors.
Journal of Pharmacology and Experimental Therapeutics | Year: 2015
It has been reported that ophiopogonin D (OP-D), a steroidal glycoside and an active component extracted from Ophiopogon japonicas, promotes antioxidative protection of the cardiovascular system. However, it is unknown whether OP-D exerts protective effects against doxorubicin (DOX)-induced autophagic cardiomyocyte injury. Here, we demonstrate that DOX induced excessive autophagy through the generation of reactive oxygen species (ROS) in H9c2 cells and in mouse hearts, which was indicated by a significant increase in the number of autophagic vacuoles, LC3-II/LC3-I ratio, and upregulation of the expression of GFP-LC3. Pretreatment with OP-D partially attenuated the above phenomena, similar to the effects of treatment with 3-methyladenine. In addition, OP-D treatment significantly relieved the disruption of the mitochondrial membrane potential by antioxidative effects through downregulating the expression of both phosphorylated c-Jun N-terminal kinase and extracellular signal-regulated kinase. The ability of OP-D to reduce the generation of ROS due to mitochondrial damage and, consequently, to inhibit autophagic activity partially accounts for its protective effects in the hearts against DOX-induced toxicity. Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics
Xu X.,Jilin University |
Xu X.,Liaoning University |
Liang F.,Changchun Medical College |
Shi J.,Jilin University |
And 6 more authors.
Analytica Chimica Acta | Year: 2013
The hollow fiber-based stirring extraction bar liquid-liquid microextraction was applied to the extraction of hormones, including 17-α-ethinylestradiol, 17-α-estradiol, estriol, 17-β-estradiol, estrone, 17-α-hydroxyprogesterone, medroxyprogesterone, progesterone and norethisterone acetate, in milk. The present method has the advantages of both hollow fiber-liquid phase microextraction and stirring bar sorptive extraction. The stirring extraction bar was used as both the stirring bar of microextraction, and extractor of the analytes, which can make extraction, clean-up and concentration be carried out in one step. When the extraction was completed, the stirring extraction bar was easy isolated from the extraction system with the magnet. Several experimental parameters, including the type of extraction solvent, the number of hollow stirring extraction bar, extraction time, stirring speed, ionic strength, and desorption conditions were investigated and optimized. The analytes in the extract were derived and determined by gas chromatography mass spectrometry. Under optimal experimental conditions, good linearity was observed in the range of 0.20-20.00ngmL-1. The limits of detection and quantification were in the range of 0.02-0.06ngmL-1 and 0.07-0.19ngmL-1, respectively. The present method was applied to the analysis of milk samples, and the recoveries of analytes were in the range of 93.6-104.6% with the relative standard deviations ranging from 1.6% to 6.2% (n=5). The results showed that the present method was a rapid and feasible method for the determination of hormones in milk samples. © 2013 Elsevier B.V.
Ji Z.,Jilin University |
Li Y.,Changchun Medical College |
Sun G.,Jilin University
International Journal of Clinical and Experimental Medicine | Year: 2016
Resveratrol (Res) is reported to exert anti-inflammatory and anti-oxidative properties. Sepsis-induced myocardial injury is tightly associated with inflammation. However, the mechanisms underlying the protective effect of Res remain unclear. The present study investigated the protective effect of Res on myocardial injury in septic rats and the role of the Janus Kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway. Res treatment was found to significantly inhibit the activation of JAK2 and STAT3 in myocardial tissue. It also attenuated the level of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β in the serum and myocardial tissue. In addition, Res alleviated myocardial apoptosis. In conclusion, the results indicate that Res exhibits substantial therapeutic potential for the treatment of sepsis-induced myocardial injury via JAK2/STAT3 signaling inhibition. © 2016, E-Century Publishing Corporation. All Rights Reserved.
PubMed | Jilin University, Changchun Medical College and Beihua University
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2016
Diabetes mellitus (DM) is a metabolic syndrome caused by multiple genetic and environmental factors. Traditional Chinese medicine preparations have shown a comprehensive and function-regulating characteristic. Purslane (Portulaca oleracea L.) is an annual succulent herb. Currently, there have been some related reports on the treatment of diabetes with purslane. The current study was designed to separate and purify the polysaccharide, a systematic study of its physical and chemical properties, antioxidant activity, and anti-diabetic mechanism, in order to provide a theoretical basis for the development of drugs of purslane. A crude water soluble polysaccharide extracted from purslane was named CPOP (crude Portulaca oleracea L. polysaccharide). Effects of CPOP on bodyweight, glucose tolerance test (GTT), fasting blood glucose (FBG), fasting serum insulin (FINS), insulin sensitivity index (ISI), interleukin-6 (IL-6), tumor necrosis factor- (TNF-), methane dicarboxylic aldehyde (MDA), and superoxygen dehydrogenises (SOD) were investigated. The results indicate that the oral administration of CPOP could significantly increase the body weight and significantly improve the glucose tolerance in diabetic rats. Meanwhile, CPOP could significantly reduce the FBG level, and elevate the FINS level and ISI value in diabetic rats. In addition, CPOP could significantly reduce TNF- and IL-6 levels in diabetic rats; CPOP could also reduce MDA and SOD activities in the liver tissue of diabetic rats. These results suggest that the anti-diabetic effect of CPOP may be associated with its antioxidant and anti-inflammatory effects.
PubMed | Jilin University and Changchun Medical College
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016
Paecilomyces hepiali (PH), a well-known medicinal fungus, has various pharmacological efficacies. In our study, the antinociceptive effects of PH and underlying mechanisms were evaluated using various mouse models. An acetic acid-induced writhing test, hot plate test, and formalin test were employed to evaluate the antinociceptive activities of PH. The levels of neuronal nitric oxide synthase (nNOS) in the hypothalamus and monoamine neurotransmitters in the serum and hypothalamus of experimental mice were examined. Additionally, hot plate tests using mice pretreated with various antagonists were used to determine the mechanisms of PH-mediated antinociception. The PH-enhanced latency period of mice in the hot plate test was significantly blocked by pretreatment with atropine and glibenclamide. PH shortened the phase I and phase II reaction times of formalin-treated mice. Strongly reduced writhing and stretching induced by acetic acid were observed in PH-treated mice, indicating that PH mainly exerts antinociceptive activity on neurogenic pain. After thermal pain stimulation for 30 s, compared to control mice, 7-day PH-treated mice had lower nNOS and dopamine levels, and increased levels of serotonin in both the serum and hypothalamus. Collectively, our data showed that PH mediated antinociceptive activities via multiple pathways, including monoamines, nNOS/ATP-sensitive K
Li F.,Northeast Normal University |
Yu D.,Changchun Medical College |
Lin X.,Northeast Normal University |
Liu D.,Northeast Normal University |
And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2012
In this study, fungi isolated from soil were screened for their ability to form clear zones on agar plates with emulsified poly(ε-caprolactone) (PCL). The most active strain, designated as DSYD05, was identified as Penicillium oxalicum on the basis of morphological characteristics and phylogenetic analysis. Mutant DSYD05-1, obtained by ultraviolet-light mutagenesis from strain DSYD05, was more effective in PCL degradation. In liquid cultures of the mutant strain with PCL emulsion, DSYD05-1 showed the highest PCL-degrading activity after 4 days of cultivation. The products of PCL degradation were analysed by mass spectrometry; the results indicated that 6-hydroxyhexanoic acid was produced and assimilated during cultivation. The degradation of PCL film by DSYD05-1 was observed by scanning electron microscopy, and was indicative of a three-stage degradation process. The degradation of amorphous parts of the film preceded that of the crystalline center and then the peripheral crystalline regions. In addition, DSYD05-1 showed a wide range of substrate specificity, with capability to degrade PCL, poly(β-hydroxybutyrate), and poly(butylene succinate), but not poly(lactic acid), indicating that the strain could have potential for application in the treatment or recycling of bio-plastic wastes. © 2012 Springer Science+Business Media B.V.
FengQin W.,Jilin Business and Technology College |
Yang L.,Changchun Medical College
2010 International Conference on Computer, Mechatronics, Control and Electronic Engineering, CMCE 2010 | Year: 2010
By adding a wireless sensor network into a health monitoring system, I have found a method by using the combination of ZigBee and wireless LAN architecture to realize a remote medical monitoring system. The system's nodes and base stations use the short range communication standard: 802.15.4 / ZigBee standard. This system can collect various information from patients, achieving the remote medical care at home. It can promptly reflect the patient's status to doctors and the patient's families as well as coping with possible emergency situations. Not only can this system obtains accurate measurements, but also it can relieve the work for patients travelling between hospitals and home. Meanwhile we can set up a wireless monitoring network in the hospital wards which will greatly facilitate the attendance of patients. © 2010 IEEE.
PubMed | Changchun Medical College and Jiangsu University
Type: | Journal: BioMed research international | Year: 2016
Lycium barbarum, extensively utilized as a medicinal plant in China for years, exhibits antitumor, immunoregulative, hepatoprotective, and neuroprotective properties. The present study aims to investigate the hyperglycemic and antidiabetic nephritic effects of polysaccharide which is separated from Lycium barbarum (LBPS) in high-fat diet-streptozotocin- (STZ-) induced rat models. The reduced bodyweight and enhanced blood glucose concentration in serum were observed in diabetic rats, and they were significantly normalized to the healthy level by 100mg/kg of metformin (Met) and LBPS at doses of 100, 250, and 500mg/kg. LBPS inhibited albuminuria and blood urea nitrogen concentration and serum levels of inflammatory factors including IL-2, IL-6, TNF-, IFN-, MCP-1, and ICAM-1 compared with diabetic rats, and it indicates the protection on renal damage. Furthermore, the activities of SOD and GSH-Px in serum were enhanced strikingly by LBPS which suggests its antioxidation effects. LBPS, compared with nontreated diabetic rats, inhibited the expression of phosphor-nuclear factors kappa B (NF-B) and inhibitor kappa B alpha in kidney tissues. Collectively, LBPS possesses antidiabetic and antinephritic effects related to NF-B-mediated antioxidant and antiinflammatory activities.