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Changchun, China

Zhang Y.-Y.,Nanjing Normal University | Zhang Y.-Y.,Changchun Medical College | Meng C.,Nanjing Normal University | Zhang X.-M.,Jilin University | And 7 more authors.
Journal of Pharmacology and Experimental Therapeutics

It has been reported that ophiopogonin D (OP-D), a steroidal glycoside and an active component extracted from Ophiopogon japonicas, promotes antioxidative protection of the cardiovascular system. However, it is unknown whether OP-D exerts protective effects against doxorubicin (DOX)-induced autophagic cardiomyocyte injury. Here, we demonstrate that DOX induced excessive autophagy through the generation of reactive oxygen species (ROS) in H9c2 cells and in mouse hearts, which was indicated by a significant increase in the number of autophagic vacuoles, LC3-II/LC3-I ratio, and upregulation of the expression of GFP-LC3. Pretreatment with OP-D partially attenuated the above phenomena, similar to the effects of treatment with 3-methyladenine. In addition, OP-D treatment significantly relieved the disruption of the mitochondrial membrane potential by antioxidative effects through downregulating the expression of both phosphorylated c-Jun N-terminal kinase and extracellular signal-regulated kinase. The ability of OP-D to reduce the generation of ROS due to mitochondrial damage and, consequently, to inhibit autophagic activity partially accounts for its protective effects in the hearts against DOX-induced toxicity. Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics Source

Ji Z.,Jilin University | Li Y.,Changchun Medical College | Sun G.,Jilin University
International Journal of Clinical and Experimental Medicine

Resveratrol (Res) is reported to exert anti-inflammatory and anti-oxidative properties. Sepsis-induced myocardial injury is tightly associated with inflammation. However, the mechanisms underlying the protective effect of Res remain unclear. The present study investigated the protective effect of Res on myocardial injury in septic rats and the role of the Janus Kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway. Res treatment was found to significantly inhibit the activation of JAK2 and STAT3 in myocardial tissue. It also attenuated the level of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β in the serum and myocardial tissue. In addition, Res alleviated myocardial apoptosis. In conclusion, the results indicate that Res exhibits substantial therapeutic potential for the treatment of sepsis-induced myocardial injury via JAK2/STAT3 signaling inhibition. © 2016, E-Century Publishing Corporation. All Rights Reserved. Source

Xue H.,Jilin University | Zhang X.-Y.,Jilin University | Liu J.-M.,Jilin University | Song Y.,Jilin University | And 3 more authors.
Brain Research

After spinal cord injury (SCI), a series of complex pathophysiological processes follows the initial injury. Because inflammation plays a key role in this secondary pathology damage, anti-inflammatory drug treatment may reduce secondary damage and protect neurons after SCI. Though nordihydroguaiaretic acid (NDGA) can inhibit inflammatory responses, its potential roles in neuroprotection and anti- inflammation in an SCI model have not been studied. In this study, we investigated the anti-inflammatory effects of NDGA in SCI. First, histopathological alterations were evaluated with hematoxylin/eosin (HE) and Nissl staining, showing an increased number of neurons after NDGA administration. Additionally, the extent of secondary damage was assessed by TUNEL assay and measurement of astrocyte proliferation. The data showed that the numbers of apoptotic cells and the proliferative extent of astrocytes were significantly decreased by the use of NDGA. The anti-inflammatory effect of NDGA was evaluated by measuring myeloperoxidase (MPO) levels as an indicator of neutrophil activity, macrophage/microglia numbers, and expression of inflammatory cytokines including IL-1β and TNF-α. NDGA treatment significantly decreased the MPO level and the number of macrophages/microglia. In addition, NDGA also suppressed the expression of IL-1β and TNF-α after SCI. These data suggest that anti-inflammatory action by NDGA can reduce secondary damage after SCI © 2013 Elsevier B.V. All rights reserved. Source

Xue H.,Jilin University | Zhang X.-Y.,Jilin University | Liu J.-M.,Jilin University | Song Y.,Jilin University | And 3 more authors.
Journal of Biomedical Materials Research - Part A

Bridging strategies are essential for spinal cord repair in order to provide a physical substrate allowing axons to grow across the site of spinal cord lesions. In this study, we have evaluated the therapeutic effects of adding amniotic epithelial cells to a unidirectionally oriented acellular muscle scaffold and have compared this with the effect of a scaffold alone. Chemically extracted acellular muscles, with or without amniotic epithelial cells, were implanted into the lateral hemisected adult rat thoracic spinal cord. Control rats were similarly injured. After 4 weeks, the acellular muscle scaffolds were found to be well integrated with the host tissue. The chemically extracted acellular muscle scaffold seeded with amniotic epithelial cells promoted axonal growth in a distinctly organized and linear fashion, induced sprouting of calcitonin gene-related peptide positive axons, and was not associated with an astrocyte response. Compared with acellular muscle scaffolds alone, the addition of amniotic epithelial cells further promoted the remyelination of nerve fibers, sprouting of 5-hydroxytryptamine nerve fibers, relays of cortical motor-evoked potential and cortical somatosensory-evoked potential, and functional recovery. All these data together suggest that co-implantation of chemically extracted acellular muscle with amniotic epithelial cells may constitute a valuable approach to study and/or develop therapies for spinal cord injury. © 2012 Wiley Periodicals, Inc. Source

Li F.,Northeast Normal University | Yu D.,Changchun Medical College | Lin X.,Northeast Normal University | Liu D.,Northeast Normal University | And 2 more authors.
World Journal of Microbiology and Biotechnology

In this study, fungi isolated from soil were screened for their ability to form clear zones on agar plates with emulsified poly(ε-caprolactone) (PCL). The most active strain, designated as DSYD05, was identified as Penicillium oxalicum on the basis of morphological characteristics and phylogenetic analysis. Mutant DSYD05-1, obtained by ultraviolet-light mutagenesis from strain DSYD05, was more effective in PCL degradation. In liquid cultures of the mutant strain with PCL emulsion, DSYD05-1 showed the highest PCL-degrading activity after 4 days of cultivation. The products of PCL degradation were analysed by mass spectrometry; the results indicated that 6-hydroxyhexanoic acid was produced and assimilated during cultivation. The degradation of PCL film by DSYD05-1 was observed by scanning electron microscopy, and was indicative of a three-stage degradation process. The degradation of amorphous parts of the film preceded that of the crystalline center and then the peripheral crystalline regions. In addition, DSYD05-1 showed a wide range of substrate specificity, with capability to degrade PCL, poly(β-hydroxybutyrate), and poly(butylene succinate), but not poly(lactic acid), indicating that the strain could have potential for application in the treatment or recycling of bio-plastic wastes. © 2012 Springer Science+Business Media B.V. Source

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